• 한국학교보건학회지
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• 제18권2호
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• pp.93-104
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• 2005

Purpose： This study compared lifestyles of middle school students with and without atopic dermatitis in order to obtain the baseline information for health education. Methods： This study involved 256 students from 20 middle schools in Busan who suffered from atopic dermatitis and 231 students from 2 middle schools in Busan who did not have atopic dermatitis. Results： Students with atopic dermatitis had a lower family income (탑=16.99, p<.001) and were more likely to come from a nuclear family (탑=10.28, p<.001) than control students. 98% of the students with atopic dermatitis had several skin symptoms. 78.5% of students with atopic dermatitis reported experiencing difficulties in their daily because of their condition. Students with atopic dermatitis took shorter baths and used soap less frequently than the students in the control group. The students with atopic dermatitis cleaned their houses and bedclothes more often than normal students in an attempt to remove cockroaches and house-dust mites. Students with atopic dermatitis also ate fast and instant foods more often than normal students. Conclusions： Some lifestyle differences exist between students with and without atopic dermatitis. Therefore, education and guidance may be important to promote good health and improve lifestyle quality.

• 한국게임학회 논문지
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• 제20권4호
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• pp.3-10
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• 2020

본 연구의 목적은 기존 방역시스템의 한계를 극복하기 위한 게임 기반 방역 프레임워크 설계이다. 최근 서울에서 발생한 바퀴벌레 범람 사태와 같이 바퀴벌레의 뚜렷한 출현 패턴을 파악하기 어려워지면서 기존 방역체계의 한계가 빈번하게 발생하고 있다. 특히 도시개발에서 필수적인 시설로 여겨지는 공공하수도 시설은 도시 난개발로 인해 체계적 방역사업을 진행하기 어렵다는 문제에 직면해 있다. 이러한 도시의 문제를 해결하기 위해 본 연구에서는 공공방역 설계에 게임을 접목함으로써 시민참여를 통한 집단지성을 구축하고, 바퀴벌레 방역의 대안을 도출할 수 있는 게임 기반 방역 프레임워크를 제안한다.

• Parasites, Hosts and Diseases
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• 제43권3호
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• pp.101-110
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• 2005

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bp open reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited $42-57\%$ homology with the serine protease from dust mites, and consisted of a conserved catalytic domain (GOSGGPLV). bgtryp-1 was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Western blotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia coli revealed that the molecular mass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cock-roach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the German cockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2.

• 한국항행학회논문지
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• 제24권5호
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• pp.444-449
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• 2020

본 연구에서는 구조가 간단하고 소형화 제작이 용이한 소형보행 로봇용 구동원을 제안한다. 두 개의 직교방향 모멘트를 핀조인트 구조의 다리에서 회전벡터로 변환하여 이동표면에 타원궤적이 발생하도록 설계하였고, 다른 구동원 대비 효율성과 에너지 밀도가 높은 압전 세라믹을 활용하여 압전벤더 액츄에어터를 제작하였다. 또한 사용된 압전 벤더 액츄에이터는 유한요소해석을 통한 자체 무게대비 최대의 출력을 낼 수 있도록 재질 및 형상을 최적화하였다. 두 개의 다리로 구성된 단위 액츄에이터는 모듈화 되어 여러 개의 액츄에이터로 결합이 가능하고 매운 빠른 속도로 이동 및 거친 지형에서도 운용이 가능하다. 실험결과로 T자형 로봇 액츄에이터의 다양한 속도 (2 mm/sec.에서 266 mm/sec까지)와 운송능력(최대 10 g에서 50 mm/s까지)을 확인 하였다.

• Animal Systematics, Evolution and Diversity
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• 제11권1호
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• pp.87-100
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• 1995

1994년 7월 전라남도 진도에서 위생절지동물상을 조사하였다. 모기는 8종이 채집되었는데 그중 일본뇌염 매개종인 작은빨간집모기(Culex tritaeniorhynchus)가 우점종이었다. 신종으로 보이는 Cluex sp. 유충이 채집되었는데 명명은 성충이 채집되지 않아 보류하였다. 깔따구는 모두 11속 23종이 채집되었는데 그 중 Cladopelma viridula(녹색사촌무깔따구, 신칭), Dicrotendipes septem-maculatus(여섯점갈래깔따구, 신칭) 및 Harnischia urtilamellata(혹무깔따구, 신칭)는 한국 미기록종이어서 자세히 재기재하였다. 깔따구류중 우점종은 안개무늬날개깔따구(Chironomus kiiensis)이었고(67.3%), 노란털깔따구(Ch. flaviplumus)가 그 다음이었다(15.6%), 등에모기는 모두 5종이 채집되었고 그 중 Culicoides punctatus가 88.7%로 우점종이었다. Foreipomia sp.도 채집되었는데 이 속은 한국에서 처음으로 기록되는 것이다. 들쥐 채집결과는 등줄쥐(Apodemus agrarius)가 높은 밀도를 보였고 등줄쥐에 기생하는 외부기생 절지동물로 Leptotrombidium orientale, Ixodes nipponensis, Laelaps jettmani, Eulaelaps stabularis 등이 동정되었다.

• 한국산학기술학회논문지
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• 제21권4호
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• pp.356-361
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• 2020

소형의 다리형 주행 로봇은 몸체의 크기 대비 바퀴주행보다 높은 장애물을 극복할 수 있다는 장점이 있다. 이러한 이점을 활용하여 다양한 형태의 다리 주행 소형 로봇이 개발되었다. 본 논문에서는 기존의 단면 주행을 넘어 양면 주행이 가능한 12족 생체모사 크롤링 로봇을 제안한다. 주행 로봇은 험지 주행 시에 전복되거나 자세가 흐트러지는 경우가 자주 발생한다. 12족 양면 주행로봇은 전복이 되어도 지체 없이 곧바로 주행이 계속 가능하다. 양면 주행을 위한 핵심 메커니즘은 구동부를 공유하는 것이다. 몸체부의 중간에 위치한 힘 전달 구조가 위와 아래에 위치한 모든 다리로 움직임을 전달한다. 이와 같이, 유사한 기능을 수행하는 구조물을 통합하여 구조의 간소화와 경량화를 달성하였다. 또한, 무게를 줄이기 위하여 종이 기반의 복합재를 사용하여 32g의 경량 설계를 수행하였다. 로봇의 성능을 평가하기 위해, 윗면과 아랫면 모두에 대해 주행 테스트를 실시하였다. 그 결과 윗면으로 주행 시 0.52m/s, 아랫면으로 주행 시 0.42m/s의 속력을 보여주어, 양면 모두 성공적인 주행이 가능함을 확인하였다.

• Parasites, Hosts and Diseases
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• 제47권1호
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• pp.31-36
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• 2009

Cockroaches have been recognized as a major cause of asthma. Bla g 4 is one of the most important German cockroach allergens. The aim of this study is to investigate IgE reactivity to the recombinant Bla g 4 (rBla g 4) in the sera of allergic patients and identify linear IgE binding epitope. For protein expression, full-length Bla g 4 (EF202172) was divided into 5 overlapping peptide fragments (E1: aa 1-100, E2: aa 34-77, E3: aa 74-117, E4: aa 114-156, and E5: aa 153-182). The full-length and 5 peptide fragments of Bla g 4 was generated by PCR and over-expressed in E. coli BL21 (DE3). The IgE binding reactivities of the full-length and peptide fragments were measured by ELISA using 32 serum samples of cockroach allergy. The sera of 8 patients (25%) reacted with rBla g 4. Four sera (100%) showed IgE-binding reactivity to full-length and peptide fragment 4, and 2 sera (50%) reacted with peptide fragment 2. One (20%) serum reacted with peptide fragment 3. The results of ELISA using overlapping recombinant fragments indicated that the epitope region was located at amino acid sequences 34-73 and 78-113, and major IgE epitope of Bla g 4 was located at amino acid sequences 118-152 of C-terminal. B-cell epitope analysis of German cockroach allergen Bla g 4 could contribute to the strategic development of more specific and potentially efficacious immunotherapy.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1343-1349
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• 2021

Cockroaches live in places where various pathogens exist and thus are more likely to use antimicrobial compounds to defend against pathogen intrusions. We previously performed an in silico analysis of the Periplaneta americana transcriptome and detected periplanetasin-5 using an in silico antimicrobial peptide prediction method. In this study, we investigated whether periplanetasin-5 has anticancer activity against the human leukemia cell line K562. Cell growth and survival of K562 cells treated with periplanetasin-5 were decreased in a dose-dependent manner. By using flow cytometric analysis, acridine orange/ethidium bromide (AO/EB) staining and DNA fragmentation, we found that periplanetasin-5 induced apoptotic and necrotic cell death in leukemia cells. In addition, these events were associated with increased levels of the pro-apoptotic proteins Fas and cytochrome c and reduced levels of the anti-apoptotic protein Bcl-2. Periplanetasin-5 induces the cleavage of pro-caspase-9, pro-caspase-8, pro-caspase-3, and poly (ADP-ribose) polymerase (PARP). The above data suggest that periplanetasin-5 induces apoptosis via both the intrinsic and extrinsic pathways. Moreover, caspase-related apoptosis was further confirmed by using the caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK), which reversed the periplanetasin-5-induced reduction in cell viability. In conclusion, periplanetasin-5 caused apoptosis in leukemia cells, suggesting its potential utility as an anticancer therapeutic agent.

• Parasites, Hosts and Diseases
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• 제60권4호
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• pp.289-293
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• 2022

Blastocystis is a genus of unicellular heterokont parasites belonging to a group of organisms known as Stramenopiles, which includes algae, diatoms, and water molds. Blastocystis includes several species that habitat in the gastrointestinal tracts of organisms as diverse as humans, farm animals, birds, rodents, reptiles, amphibians, fish, and cockroaches. It is important to public health and distributed globally, but its prevalence in dogs in Korea has not been reported to date. Here, we collected 787 canine fecal samples and assessed Blastocystis infection by age, sex, region, season, and diarrhea symptoms. We determined Blastocystis subtypes using phylogenetic analyses based on 18S rRNA gene sequences. We identified, 10 Blastocystis positive samples (1.3%). A higher proportion of infected dogs was asymptomatic; however, infection rates did not significantly differ according to region, age, sex, and season. Phylogenetic analysis showed that the Blastocystis sp. identified belonged to 4 subtypes (STs), ST1, ST5, ST10, and ST14, thus revealed the genetic diversity of Blastocystis sp. in dogs Korean. This is first report on the presence of Blastocystis sp. in dogs Korean. This study revealed a lower infection rate than expected and differed from previous studies in STs. Further studies are warranted to observe the national infection status of Blastocystis in dogs and the genetic characteristics of this genus.

• Parasites, Hosts and Diseases
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• 제61권2호
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• pp.202-209
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• 2023

Lophomonas blattarum is an anaerobic protozoan living in the intestine of cockroaches and house dust mites, with ultramicroscopic characteristics such as the presence of a parabasal body, axial filament, and absence of mitochondria. More than 200 cases of Lophomonas infection of the respiratory tract have been reported worldwide. However, the current diagnosis of such infection depends only on light microscopic morphological findings from respiratory secretions. In this study, we attempted to provide more robust evidence of protozoal infection in an immunocompromised patient with atypical pneumonia, positive for Lophomonas-like protozoal cell forms. A direct search of bronchoalveolar lavage fluid via polymerase chain reaction (PCR), transmission electron microscopy (TEM), and metagenomic next-generation sequencing did not prove the presence of protozoal infection. PCR results were not validated with sufficient rigor, while de novo assembly and taxonomic classification results did not confirm the presence of an unidentified pathogen. The TEM results implied that such protozoal forms in light microscopy are actually non-detached ciliated epithelial cells. After ruling out infectious causes, the patient's final diagnosis was drug-induced pneumonitis. These findings underscore the lack of validation in the previously utilized diagnostic methods, and more evidence in the presence of L. blattarum is required to further prove its pathogenicity.