• Korean Journal of Poultry Science
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• v.38 no.4
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• pp.323-330
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• 2011

The chicken monoclonal antibody (mAb), 13C8, reacts with sporozoite antigens of Eimeria spp. which causes avian coccidiosis. Since this mAb was produced at low amount due to genetic instability of chicken hybridoma, a recombinant 13C8 single-chain Fv (ScFv) antibody was constructed by amplification of the variable domain of heavy (VH) and light chain (VL) genes of antibody derived from chicken hybridoma. The constructed 13C8 ScFv was successfully expressed in E. coli and purified as a soluble form. In ELISA analysis, this recombinant 13C8 ScFv antibody showed antigen binding activity as the original mAb. In addition, nucleotide sequence comparison of 13C8 gene to the germline chicken VL and VH genes suggested that the gene conversion with $V{\lambda}$ and VH pseudogenes might contribute to the diversification of VL and VH genes in chickens.

• Journal of Veterinary Clinics
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• v.29 no.2
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• pp.154-159
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• 2012

The efficacy of water soluble formulation of diclazuril (Coccimuel-S 0.5%) was tested against $Eimeria$ spp. infection broiler chickens. The experiment was performed both experimentally infection and in the field test. Coccimuel-S composed with diclazuril induced a marked inhibitory effect on the different stages of $Eimeria$ life cycle in experimentally infected broiler chickens treated with the drug. The tested dosage levels of Coccimuel-S (0.5 ml/L, equivalent to diclazuril 2.5 ppm) in drinking water showed the significant effect compared with the control group in controlling coccidial infection and reducing the total oocyst numbers, lesion and fecal scores ($p$ < 0.001). In addition, testing of Coccimuel-S (0.25 and 0.5 ml/L) in naturally infected poultry farms (1,200 broiler chickens), showed the significant anticoccidial effect compared to control ($p$ < 0.001). In conclusion, addition of Coccimuel-S at the dose of 0.25 and 0.5 ml/L in the drinking water, induced efficacious effect for the treatment of coccidiosis in naturally coccidia infected broiler chickens.

• Journal of the korean veterinary medical association
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• v.15 no.7
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• pp.385-387
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• 1979

When 14 rabbits .were treated intraperitoneally 5ml of 20ml/100ml ethanol solution per kg body weight and physiological saline of 5ml to 19 rabbits in the same rate of death produced by coccidium infection was significantly decreased in the ethanol treate

• Proceedings of the Korean Society of Veterinary Service Conference
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• 2001.06a
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• pp.185.2-186
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• 2001

• Korean Journal of Veterinary Pathology
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• v.6 no.2
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• pp.73-77
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• 2002

고양이를 제외한 포유동물 및 조류는 oocyst 또는 cyst의 섭취로 감염되고 무성생식을 한다. 급성기에는 tachyzoite ($2~3{\times}5~7$\;\mu\textrm{m}$)가 여러 장기에서, 숙주 변역이 활성화된 이후인 만성기에는 100~1,000 개의 bradyzoite를 내장하며 주변에 염증반응이 없는 cyst (직경 $20~50;\mu\textrm{m}$)가 근육 및 뇌에서 관찰된다. Tachyzoite는 태반을 통해 태아 감염을 일으킨다. 고양이는 oocyst 또는 cyst의 섭취로 감염되고, 기타 동물에서 보이는 감염양식 외에 소장에서 coccidiosis양의 증식이 이루어짐으로 oocyst (Isospora 형, $10{\times}12;\mu\textrm{m}$)를 배출한다.

• Korean Journal of Veterinary Research
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• v.10 no.2
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• pp.73-80
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• 1970

This report summarized the results of the post-mortem diagnosis oil the 1,785 fowl live and dead during the calender year of 1966 to 1968. The disease incidences in order were; leucosis complex(21.9%) newcastle disease (10.4%), coccidiosis (6.6%) and mismanagement(3.9%). There were also a number of other diseases common to tb,e poultry flocks such as avian mycoplasmosis, pullorum disease and, nutritional disorder etc.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1515-1521
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• 2016

Anethole and garlic have an immune modulatory effects on avian coccidiosis, and these effects are correlated with gene expression changes in intestinal epithelial lymphocytes (IELs). In this study, we integrated gene expression datasets from two independent experiments and investigated gene expression profile changes by anethole and garlic respectively, and identified gene expression signatures, which are common targets of these herbs as they might be used for the evaluation of the effect of plant herbs on immunity toward avian coccidiosis. We identified 4,382 and 371 genes, which were differentially expressed in IELs of chickens supplemented with garlic and anethole respectively. The gene ontology (GO) term of differentially expressed genes (DEGs) from garlic treatment resulted in the biological processes (BPs) related to proteolysis, e.g., "modification-dependent protein catabolic process", "proteolysis involved in cellular protein catabolic process", "cellular protein catabolic process", "protein catabolic process", and "ubiquitin-dependent protein catabolic process". In GO analysis, one BP term, "Proteolysis", was obtained. Among DEGs, 300 genes were differentially regulated in response to both garlic and anethole, and 234 and 59 genes were either up- or down-regulated in supplementation with both herbs. Pathway analysis resulted in enrichment of the pathways related to digestion such as "Starch and sucrose metabolism" and "Insulin signaling pathway". Taken together, the results obtained in the present study could contribute to the effective development of evaluation system of plant herbs based on molecular signatures related with their immunological functions in chicken IELs.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.165-168
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• 1999

To determine the effects of genetic and environmental influences on cell mediated immune (CMI) responses in broiler rabbits, contact sensitivity to 2,4-Dinitrochlorobenzene (DNCB) was assessed in three temperate broiler breeds of rabbits, namely Soviet Chinchilla, New Zealand White and Grey Giant. The feasibility of using the contact sensitivity to DNCB as a marker trait in selection for disease resistance was examined. There were highly significant differences between breeds (p<0.01) in initial skin thickness and contact sensitivities to DNCB at 24, 48 and 72 hours. Initial skin thickness was greatest in the Soviet Chinchilla breed (mean 2.2484 mm), and was significantly greater (p<0.01) in males (2.4963 mm) than in females (1.7846 mm) (p<0.01). Highest contact sensitivity to DNCB was in the New Zealand White breed with mean increase in skin thickness of 1.1884, 0.9072 and 0.5879 mm at 24, 48 and 72 hours post challenge respectively. Delayed type hypersensitivity (DTH) reaction to DNCB at 24 hours post challenge had a highly significant association (p<0.01) with the incidence of body mange in rabbits. The results indicated a lowered contact sensitivity to DNCB at 24 hours post challenge was associated significantly (p<0.01) with an increase in incidence and severity of body mange, suggesting its potential value as a marker. The correlation s among contact sensitivities at 24, 48 and 72 hours were positive and highly significant (p<0.01); correlations between initial skin thickness and contact sensitivities were negative and highly significant (p<0.01). Another notable significant correlation was between body weight and delayed type hypersensitivity at 24 hours indicating that an enhanced CMI might be associated with better growth rate and general wellbeing.

• Journal of Veterinary Clinics
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• v.12 no.1
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• pp.911-916
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• 1995

Ionophorous antibiotics have been used popularly for the protection of avian coccidiosis, though Halo-fuginone which is derived from an extract of the Dichroa febrifuga, was developed as an antimalarial and anticoccidial agent. The antibiotics are regarded as the causes of residues in the avian products, therefore the author has tried to find out more safe herbal materials for the control of avian coccidiosis. Thus, the extracts of 5 kinds of herbs, roots of Pulsatilla koreana Nakai and Sophora flavescens Aiton, nuts of Quisqualis indica Linne, whole herbs of Artemisia annua Linne and Polygonum aviculare Linne, were investigated on the efficacy against E tenella. Survival rates, bloody diarrhea, lesion scores, body-weight gains and feed conversion rate were investigated at the 1st and the 2nd week after infection. The bloody diarrhea in the groups treated with P koreana, A annua, Q indica and P aviculare were milder than those in the groups treated with S flavescens and infected control. The lesion score in the groups treat.4 with herb extracts(2.00$\pm$0.82~2.65$\pm$0.89) were lower than those in the control group (2.80$\pm$0.7l). But, there was not significant in all infected groups. The body weight gai in the groups treated with P koreana, S flavescens, A annua and Q indica(291.74$\pm$15.76~303.43$\pm$51.90) were higher than that in the control group (283.71$\pm$16.53) but there was not significant. In a conclusion, analyzing the data of the survival rates, bloody diarrheal symptoms, lesion scores, body weight gains and oocyst excretions, the extract of S flavescens, P koreana, A annua and Q indica were effective. The further research on the above herbal materials will have been carried out by the author and the other researchers by means of the chemical analysis of the components.

• Korean Journal of Veterinary Service
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• v.27 no.4
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• pp.371-378
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• 2004

The poultry farmers of rural area in Bangladesh usually prefer raising cockerel to broiler due to availability, low price of chicks, requirement of less space and feed, and high price of meat and the farmers believe that the cockerels are less susceptible to diseases in comparison to broilers. This study was carried out to observe the pathology of diseased cockerel in 3 farms of rural area in Bangladesh. A total of 974 birds were examined and the diagnoses of different disease/conditions were based on the history, clinical signs, characteristic gross, tissue alterations, clinical pathology and isolation and identification of the pathogenic organisms. The diseases in this study included infectious bursal disease, yolk sac infection, vitamin E deficiency, coccidiosis, and other diseases. The proportionate mortality rate were $7.29\%,\;0.62\%,\;0.72\%,\;0.21\%\;and\;0.10\%$, respectively, which indicated that most of the fatal causes of death were due to infectious bursal disease. Age group of 2-8 week old were the most susceptible to this disease and E coli was suggested as a cause of yolk sac infection. The data also suggested that the coccidiosis in rural areas of Bangladesh has decreased due to awareness of the farmers and routine use of coccidiostates.