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http://dx.doi.org/10.5536/KJPS.2011.38.4.323

The Development of Chicken Recombinant Single-chain Fv (ScFv) Antibody Reactive with Sporozoite Antigen of Eimeria spp. which Causes Avian Coccidiosis  

Park, Dong-Woon (Department of Microbiology, College of Natural Sciences, Changwon National University)
Kim, Eon-Dong (Department of Microbiology, College of Natural Sciences, Changwon National University)
Kim, Sung-Heon (Department of Microbiology, College of Natural Sciences, Changwon National University)
Han, Jae-Yong (WCU Biomodulation, Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University)
Kim, Jin-Kyoo (Department of Microbiology, College of Natural Sciences, Changwon National University)
Publication Information
Korean Journal of Poultry Science / v.38, no.4, 2011 , pp. 323-330 More about this Journal
Abstract
The chicken monoclonal antibody (mAb), 13C8, reacts with sporozoite antigens of Eimeria spp. which causes avian coccidiosis. Since this mAb was produced at low amount due to genetic instability of chicken hybridoma, a recombinant 13C8 single-chain Fv (ScFv) antibody was constructed by amplification of the variable domain of heavy (VH) and light chain (VL) genes of antibody derived from chicken hybridoma. The constructed 13C8 ScFv was successfully expressed in E. coli and purified as a soluble form. In ELISA analysis, this recombinant 13C8 ScFv antibody showed antigen binding activity as the original mAb. In addition, nucleotide sequence comparison of 13C8 gene to the germline chicken VL and VH genes suggested that the gene conversion with $V{\lambda}$ and VH pseudogenes might contribute to the diversification of VL and VH genes in chickens.
Keywords
chicken monoclonal antibody; avian coccidiosis; single-chain Fv (ScFv); hybridoma; gene conversion;
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1 Sasai K, Lillehoj HS, Matsuda H, Wergin WP 1996 Characterization of a chicken monoclonal antibody that recognizes the apical complex of Eimeria acervulina sporozoites and partially inhibits sporozoite invasion of $CD8^+$ T lymphocytes in vitro. J Parasitol 82:82-87.
2 Verma R, Boleti E, George AJ 1998 Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. J Immunol Methods 216:165-181.   DOI   ScienceOn
3 Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR 1994 Making antibodies by phage display technology. Annu Rev Immunol 12:433-455.   DOI   ScienceOn
4 Bird RE, Hardman KD, Jacobson JW, Johnson S, Kaufman BM, Lee SM, Lee T, Pope SH, Riordan GS, Whitlow M 1988 Single-chain antigen-binding proteins. Science 242:423-426.   DOI
5 Chapman HD 1993 Resistance to anticoccidial drugs in fowl. Parasitol Today 9:159-162.   DOI   ScienceOn
6 Hayden MS, Gilliland LK, Ledbetter JA 1997 Antibody engineering. Curr Opin Immunol 9:201-212.   DOI   ScienceOn
7 Huston JS, Mudgett-Hunter M, Tai MS, McCartney J, Warren F, Haber E, Oppermann H 1991 Protein engineering of singlechain Fv analogs and fusion proteins. Methods Enzymol 203:46-88.   DOI
8 Kabat EA, Wu TT, Perry HM, Gottesman KS, Foeller C 1991 Sequences of proteins of immunological interest. U.S. Dept Health and Human Services, NIH publication No. 91-3242. 5th ed.
9 Kim JK, Tsen MF, Ghetie V, Ward ES 1994 Identifying amino acid residues that influence plasma clearance of murine IgG1 fragments by site-directed mutagenesis. Eur J Immunol 24:542-548.   DOI   ScienceOn
10 Yamanaka HI, Inoue T, Ikeda-Tanaka O 1996 Chicken monoclonal antibody isolated by a phage display system. J Immunol 157:1156-1162.
11 Nishinaka S, Akiba H, Nakamura M, Suzuki K, Suzuki T, Tsubokura K, Horiuchi H, Furusawa S, Matsuda H 1996 Two chicken B cell lines resistant to ouabain for the production of chicken monoclonal antibodies. J Vet Med Sci 58:1053-1056.   DOI
12 Lawn AM, Rose ME 1982 Mucosal transport of Eimeria tenella in the cecum of the chicken. J Parasitol 68:1117-1123.   DOI   ScienceOn
13 Lillehoj HS, Lillehoj EP. 2000 Avian coccidiosis. A review of acquired intestinal immunity and vaccination strategies. Avian Dis 44:408-425.   DOI   ScienceOn
14 McCormack WT, Thompson CB 1990 Chicken IgL variable region gene conversions display pseudogene donor preference and 5' to 3' polarity. Genes Dev 4:548-558.   DOI   ScienceOn
15 Reynaud CA, Anquez V, Grimal H, Weill JC 1987 A hyperconversion mechanism generates the chicken light chain preimmune repertoire. Cell 48:379-388.   DOI   ScienceOn
16 Reynaud CA, Dahan A, Anquez V, Weill JC 1989 Somatic hyperconversion diversifies the single Vh gene of the chicken with a high incidence in the D region. Cell 59:171-183.   DOI   ScienceOn