• 한국가금학회지
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• 제38권4호
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• pp.323-330
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• 2011

닭의 단일 클론 항체인 13C8 항체는 조류의 콕시듐증을 유발하는 것으로 알려진 Eimeria acervulina의 포자충(sporozoites) 항원에 결합하는 닭 항체이다. 그러나 닭 하이브리도마 유전자의 불안정성 때문에 분비되는 항체의 생산량이 낮은 단점이 있다. 이러한 단점을 보완하기 위해 hybridoma로 부터 항체의 중사슬 가변 부위(VH)유전자와 경사슬 가변 부위(VL) 유전자를 증폭하여 linker peptide로 연결해준 재조합 ScFv 항체 유전자를 구축하고, E. coli를 형질 전환시켜 재조합 단백질로 발현 정제하였다. ELISA 분석 결과 재조합 13C8 ScFv 항체는 세 종류의 Eimeria spp.에 대해 모두 항원 결합력을 나타내었으며, 염기서열 분석을 수행하여 germline sequence와 비교한 결과 닭 항체유전자의 다양성(diversity)은 pseudogene들의 gene conversion 기작에 의해 이루어짐을 제시해 주었다.

• 한국임상수의학회지
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• 제29권2호
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• pp.154-159
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• 2012

디클라쥬릴의 음수형 제제인 콕시멸-에스의 항콕시듐 효과를 Eimeria spp. 감염 육계를 이용하여 수행하였다. 본 실험은 실험실 및 야외적용시험으로 나누어 진행하였으며, 실험실 실험에서는 Eimeria spp.를 인공 감염시킨 육계를 대상으로 콕시멸-에스 0.5 ml/L을 음수로 투여한 군에서 대조군과 비교하여 콕시듐 치료, 콕시듐 충란수의 감소, 그리고 장상해도 및 분변 설사지수 등에 있어서 유의성 있는 효과를 나타내었다($p$ < 0.001). 또한, 야외적용실험에서는, 콕시듐증에 걸린 육계를 대상으로 콕시멸-에스를 0.25와 0.5 ml/L로 각각 음수로 투여한 결과, 대조군과 비교하여 모두 유의한 콕시듐 치료효과를 나타내었다($p$ < 0.001). 이상의 결과로부터, 콕시멸-에스 0.25와 0.5 ml/L를 음수로 콕시듐증에 걸린 육계에 투여할 경우, 콕시듐증 치료에 매우 효과적일 것으로 판단된다.

• 대한수의사회지
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• 제15권7호
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• pp.385-387
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• 1979

When 14 rabbits .were treated intraperitoneally 5ml of 20ml/100ml ethanol solution per kg body weight and physiological saline of 5ml to 19 rabbits in the same rate of death produced by coccidium infection was significantly decreased in the ethanol treate

• 한국가축위생학회:학술대회논문집
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• 한국가축위생학회 2001년도 제24차 학술대회
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• pp.185.2-186
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• 2001

• 한국수의병리학회지
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• 제6권2호
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• pp.73-77
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• 2002

고양이를 제외한 포유동물 및 조류는 oocyst 또는 cyst의 섭취로 감염되고 무성생식을 한다. 급성기에는 tachyzoite ($2~3{\times}5~7$\;\mu\textrm{m}$)가 여러 장기에서, 숙주 변역이 활성화된 이후인 만성기에는 100~1,000 개의 bradyzoite를 내장하며 주변에 염증반응이 없는 cyst (직경 $20~50;\mu\textrm{m}$)가 근육 및 뇌에서 관찰된다. Tachyzoite는 태반을 통해 태아 감염을 일으킨다. 고양이는 oocyst 또는 cyst의 섭취로 감염되고, 기타 동물에서 보이는 감염양식 외에 소장에서 coccidiosis양의 증식이 이루어짐으로 oocyst (Isospora 형, $10{\times}12;\mu\textrm{m}$)를 배출한다.

• 대한수의학회지
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• 제10권2호
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• pp.73-80
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• 1970

This report summarized the results of the post-mortem diagnosis oil the 1,785 fowl live and dead during the calender year of 1966 to 1968. The disease incidences in order were; leucosis complex(21.9%) newcastle disease (10.4%), coccidiosis (6.6%) and mismanagement(3.9%). There were also a number of other diseases common to tb,e poultry flocks such as avian mycoplasmosis, pullorum disease and, nutritional disorder etc.

• Asian-Australasian Journal of Animal Sciences
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• 제29권10호
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• pp.1515-1521
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• 2016

Anethole and garlic have an immune modulatory effects on avian coccidiosis, and these effects are correlated with gene expression changes in intestinal epithelial lymphocytes (IELs). In this study, we integrated gene expression datasets from two independent experiments and investigated gene expression profile changes by anethole and garlic respectively, and identified gene expression signatures, which are common targets of these herbs as they might be used for the evaluation of the effect of plant herbs on immunity toward avian coccidiosis. We identified 4,382 and 371 genes, which were differentially expressed in IELs of chickens supplemented with garlic and anethole respectively. The gene ontology (GO) term of differentially expressed genes (DEGs) from garlic treatment resulted in the biological processes (BPs) related to proteolysis, e.g., "modification-dependent protein catabolic process", "proteolysis involved in cellular protein catabolic process", "cellular protein catabolic process", "protein catabolic process", and "ubiquitin-dependent protein catabolic process". In GO analysis, one BP term, "Proteolysis", was obtained. Among DEGs, 300 genes were differentially regulated in response to both garlic and anethole, and 234 and 59 genes were either up- or down-regulated in supplementation with both herbs. Pathway analysis resulted in enrichment of the pathways related to digestion such as "Starch and sucrose metabolism" and "Insulin signaling pathway". Taken together, the results obtained in the present study could contribute to the effective development of evaluation system of plant herbs based on molecular signatures related with their immunological functions in chicken IELs.

• Asian-Australasian Journal of Animal Sciences
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• 제12권2호
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• pp.165-168
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• 1999

To determine the effects of genetic and environmental influences on cell mediated immune (CMI) responses in broiler rabbits, contact sensitivity to 2,4-Dinitrochlorobenzene (DNCB) was assessed in three temperate broiler breeds of rabbits, namely Soviet Chinchilla, New Zealand White and Grey Giant. The feasibility of using the contact sensitivity to DNCB as a marker trait in selection for disease resistance was examined. There were highly significant differences between breeds (p<0.01) in initial skin thickness and contact sensitivities to DNCB at 24, 48 and 72 hours. Initial skin thickness was greatest in the Soviet Chinchilla breed (mean 2.2484 mm), and was significantly greater (p<0.01) in males (2.4963 mm) than in females (1.7846 mm) (p<0.01). Highest contact sensitivity to DNCB was in the New Zealand White breed with mean increase in skin thickness of 1.1884, 0.9072 and 0.5879 mm at 24, 48 and 72 hours post challenge respectively. Delayed type hypersensitivity (DTH) reaction to DNCB at 24 hours post challenge had a highly significant association (p<0.01) with the incidence of body mange in rabbits. The results indicated a lowered contact sensitivity to DNCB at 24 hours post challenge was associated significantly (p<0.01) with an increase in incidence and severity of body mange, suggesting its potential value as a marker. The correlation s among contact sensitivities at 24, 48 and 72 hours were positive and highly significant (p<0.01); correlations between initial skin thickness and contact sensitivities were negative and highly significant (p<0.01). Another notable significant correlation was between body weight and delayed type hypersensitivity at 24 hours indicating that an enhanced CMI might be associated with better growth rate and general wellbeing.

• 한국임상수의학회지
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• 제12권1호
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• pp.911-916
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• 1995

Ionophorous antibiotics have been used popularly for the protection of avian coccidiosis, though Halo-fuginone which is derived from an extract of the Dichroa febrifuga, was developed as an antimalarial and anticoccidial agent. The antibiotics are regarded as the causes of residues in the avian products, therefore the author has tried to find out more safe herbal materials for the control of avian coccidiosis. Thus, the extracts of 5 kinds of herbs, roots of Pulsatilla koreana Nakai and Sophora flavescens Aiton, nuts of Quisqualis indica Linne, whole herbs of Artemisia annua Linne and Polygonum aviculare Linne, were investigated on the efficacy against E tenella. Survival rates, bloody diarrhea, lesion scores, body-weight gains and feed conversion rate were investigated at the 1st and the 2nd week after infection. The bloody diarrhea in the groups treated with P koreana, A annua, Q indica and P aviculare were milder than those in the groups treated with S flavescens and infected control. The lesion score in the groups treat.4 with herb extracts(2.00$\pm$0.82~2.65$\pm$0.89) were lower than those in the control group (2.80$\pm$0.7l). But, there was not significant in all infected groups. The body weight gai in the groups treated with P koreana, S flavescens, A annua and Q indica(291.74$\pm$15.76~303.43$\pm$51.90) were higher than that in the control group (283.71$\pm$16.53) but there was not significant. In a conclusion, analyzing the data of the survival rates, bloody diarrheal symptoms, lesion scores, body weight gains and oocyst excretions, the extract of S flavescens, P koreana, A annua and Q indica were effective. The further research on the above herbal materials will have been carried out by the author and the other researchers by means of the chemical analysis of the components.

• 한국동물위생학회지
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• 제27권4호
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• pp.371-378
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• 2004

The poultry farmers of rural area in Bangladesh usually prefer raising cockerel to broiler due to availability, low price of chicks, requirement of less space and feed, and high price of meat and the farmers believe that the cockerels are less susceptible to diseases in comparison to broilers. This study was carried out to observe the pathology of diseased cockerel in 3 farms of rural area in Bangladesh. A total of 974 birds were examined and the diagnoses of different disease/conditions were based on the history, clinical signs, characteristic gross, tissue alterations, clinical pathology and isolation and identification of the pathogenic organisms. The diseases in this study included infectious bursal disease, yolk sac infection, vitamin E deficiency, coccidiosis, and other diseases. The proportionate mortality rate were $7.29\%,\;0.62\%,\;0.72\%,\;0.21\%\;and\;0.10\%$, respectively, which indicated that most of the fatal causes of death were due to infectious bursal disease. Age group of 2-8 week old were the most susceptible to this disease and E coli was suggested as a cause of yolk sac infection. The data also suggested that the coccidiosis in rural areas of Bangladesh has decreased due to awareness of the farmers and routine use of coccidiostates.