• Restorative Dentistry and Endodontics
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• 제25권2호
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• pp.219-224
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• 2000

The purpose of this study is for evaluating the effect of MS Coat desensitizing agent in clinical situation. In this study total 60 teeth of 30 patients who is feeling hypersensitivity after periodontal surgery were treated with MS Coat desensitizing agent, and 20 teeth with saline solution for placebo effects and evaluated. All teeth were cleaned with rubber cup and pumice, after then the antibacterial agent was applied for 20 seconds and simply isolated using cotton roll Desensitizing agent was applied with a specific instrument in the manufacturers package by abrading motion for 10 seconds and re-done 8 times. Tactile stimulus with sharp explorer, air stimulus with syringe of dental unit, $7^{\circ}C$ cold water stimulus using micropipette applicator was done to evaluate hypersensitivity before apply test agent, immediate after application, 1 week after, and 3 month after application. The degree of hypersensitivity was recorded using 100mm Visual Analogue Scale and compared between group and evaluation times. From this clinical study sensitivity to the stimulus was significantly induced in both groups and MS Coat made a significant reduction in VAS score than placebo group did(p<.01). The results of this study could demonstrate that MS Coat desensitizing agent can use effectively to heat dentinal hypersensitivity.

• Journal of Animal Science and Technology
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• 제54권4호
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• pp.255-265
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• 2012

본 연구는 PCR-RFLP 기법으로 칡소의 모색 발현에 관련된 MC1R 유전자형을 분석하고, 칡소 유전자형에 따른 모색 발현 양상을 가계와 교배조합을 통하여 연구함으로써 칡소에서 호반모의 발현 비율을 증가시키는 번식 체계를 확립하고자 수행하였다. 전라북도축산위생연구소에서 사육중인 칡소 중에서 부계 또는 모계를 알고 있는 가계가 형성된 칡소로부터 혈액 또는 정자에서 genomic DNA를 추출한 후 MC1R 유전자를 증폭하였다. 증폭된 PCR product를 Msp I 제한효소로 절단하고 전기영동한 후 칡소 개체별 유전자형을 확인하였고, 이 외에도 칡소의 모색 양상과 비경의 흑색침착 정도에 따른 모색 발현 비율을 조사하여 다음과 같은 결론을 얻었다. 칡소의 모색 발현 양상은 전신호반모가 61.67%로 가장 높았고 흑모는 5.00%로 가장 낮았으며 황모는 16.67%, 부분(약반신)과 부분(일부) 호반모는 각각 8.33%로 같은 분포 양상을 보였다. 칡소의 비경 침착 양상은 3단계와 4단계가 비슷한 비율로 높았고, 다음이 2단계, 5단계, 1단계의 순이었다. 칡소의 비경에 따른 모색 발현 양상은 비경 침착이 3단계와 4단계인 칡소에서는 전신 호반모의 비율이 다른 모색 보다 높았고, 비경 침착이 1단계인 칡소는 그 중에서 황모가 80.00% (4/5)로 나타났으며, 비경 침착이 5단계인 칡소에서는 다른 단계에서 발현되지 않았던 흑모의 비율이 37.50%를 나타냈다. 칡소에는 $E^+E^+$, $E^+e$, ee 유전자형과 한우에는 ee, $E^+e$ 유전자형이 존재하였다. 칡소 MC1R 유전자형은 $E^+e$이 65.00%로 가장 높았고 $E^+E^+$은 33.33%이었고 ee도 1두로 1.67%이었다. 유전자형에 따른 모색 발현 양상을 보면, $E^+E^+$과 $E^+e$에서는 전신 호반모의 비율이 가장 높게 나타났고 ee는 황모를 나타냈다. 칡소의 가계 분석을 통한 모색 발현 양상을 조사한 결과, 부(Sire)의 유전자형이 $E^+e$ 이고 모(Dam)의 유전자형이 $E^+E^+$ 이면서 부모의 모색이 모두 전신호반모일 경우 자손의 모색은 100.00% 전신호반모였고, 부(Sire)의 유전자형이 $E^+E^+$ 이고 모(Dam)의 유전자형이 $E^+e$이면서 부모의 모색이 모두 전신호반모일 경우도 자손에서 모색의 44.44%가 호반모의 비율을 보여 두 가지의 교배조합의 경우가 자손의 전신호반모 비율을 높이는 최적의 교배 조합으로 보여진다. 이 연구의 결과를 이용하여 칡소로 판정받을 수 있는 가장 중요한 요소인 호반모의 발현비율을 증가시키는 번식 체계를 칡소 사육농가에 제시할 수 있을 것으로 사료된다.

• 한국안전학회지
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• 제20권2호
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• pp.1-5
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• 2005

An experimental study was conducted to develop an understanding of failure of ceramic coating when subjected to a thermal cycling. Number of cycles to failure were decreased as the coating thickness and the oxide of bond coat were increased. Using the finite element method, an analysis of stress distribution in ceramic coatings was performed. Radial compressive stress was increased in the top/bond coat interface with increasing coating thickness and oxide of bond coat.

• International Journal of Costume and Fashion
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• 제12권2호
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• pp.53-65
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• 2012

The scientific, professional and reliable image of doctor's coats is changing over the years, and many countries around the world are changing doctor's coat designs or removing them altogether. This study researches the history and significance of doctor's coats, and based on the research, designs and develops new doctor's coats for the Seoul National University Hospital Department of Dermatology where traditional white polyester and cotton coats are still being worn. The positive results of the study indicate that doctor's coat designs can be developed in different directions that reflect certain identities and moods while maintaining professionalism.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2004년도 춘계 학술발표대회 개요집
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• pp.223-225
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• 2004

Based on the principle of complementary energy an analytical method is developed for determining thermal stress distribution in an thermal barrier coating. This method gives the stress distributions which satisfy the stress-free boundary conditions at the edge. Numerical examples are given in order to verify the method and to investigate the thickness effects of the ZrO$_2$-8wt%Y$_2$O$_3$ top coat on the integrity of thermal barrier coating consisted of IN738LC substrate and MCrAlY bond coat.

• 한국식물병리학회지
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• 제14권4호
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• pp.314-318
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• 1998

Using the reverse transcription polymerase chain reaction (RT-PCR), a rapid and sensitive assay method for the detection and identification of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) was adapted. Two units of primers from each virus were selected and used for the determination of two different viruses. PCR fragments of BaYMV (ca. 0.9kb) and BaMMV (ca. 0.8kb) were obtained from the designed method for the assay of BaYMV and BaMMV coat protein. PT-PCR fragments were cloned using vector pT7 Blue and the sequences of the selected clones were analyzed. coat protein of BaYMV and that of BaMMV consisted of 297 amino acids (891 nucleotides) and 251 amino acids (753 nucleotides), respectively. The snalysis of coat protein genes from these two viruses showed that 45.6% of nucleotides sequence ad 34.9% of amino acid in BaYMV were homologous to those in BaMMV.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1395-1397
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• 2002

The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

• Journal of Plant Biotechnology
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• 제7권3호
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• pp.143-148
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• 2005

The coat protein mediated resistance to potato virus Y is assessed here in transgenic potato plants (Solanum tuberosum L., cv Claustar). Therefore, the corresponding cDNA from tunisian isolate of the virus was cloned into Agrobacterium tumefaciens binary vector. The transgenic lines were subsequently analysed for the presence and expression of the transgene. The CP cDNA copy number was determined for kanamycin resistant plants. Three selected transgenic lines and their S1 progeny resulting from tuber germination showed a high protection level against the virus. These data appear to support the hypothesis that the virus resistance is mediated by the translated viral coat protein expressed in transgenic potato lines.

• 한국연초학회지
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• 제18권2호
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• pp.101-106
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• 1996

Tobacco mosaic virus (TMV) tomato strain was isolated from tomato "Seo-Kwang" in Korea. The virion was purified by density gradient centrifugation, and total viral RNA was isolated from the purified particles. Coat protein (CP) cDNA of the virus was synthesized by RT-PCR, and the purified cDNA fragment was subcloned to pBluescript II SK-. The analysis of nucleotide sequence showed that this cDNA was 693 nucleotides long from the insert of clone p1571 and p1572 which contain complete codons of the viral coat protein gene (474 nucleotides) and 3' untranslated region. The nucleotides of coat protein encoding cDNA of the strain were 6 nucleotides less than that of TMV common strain isolated from tobacco plant in Korea. The CP gene showed 70% maximum homology with that of the common strain in the nucleotide level and 86% maximum homology in amino acid level.cid level.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2014년도 추계 학술논문 발표대회
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• pp.61-62
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• 2014

To evaluate anti-corrosion performance of high-frequency arc metal spray film in accordance with metal type and epoxy sealing coat application status, electrochemical impedance spectroscopy(EIS) method was conducted in this study. As a result, in case of applying Al-Mg alloy metal spray film, it showed the best polarization resistance. In case of applying epoxy sealing coat, it showed increased polarization resistance of arc metal spray film. Through this experiment, it is judged that Al-Mg alloy arc metal spray film with epoxy sealing coat can increase the biggest anti-corrosion performance of high-frequency arc metal spray film on steel.