• Title/Summary/Keyword: coat

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MC1R Genotypes, Coat Color, and Muzzle Phenotype Variation in Korean Native Brindle Cattle (MC1R 유전자의 유전자형과 칡소의 모색 발현 및 비경색 분포에 관한 연구)

  • Park, Jae-Hee;Lee, Hae-Lee;Kim, Yong-Su;Kim, Jong-Gug
    • Journal of Animal Science and Technology
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    • v.54 no.4
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    • pp.255-265
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    • 2012
  • The objectives of this study were to investigate MC1R genotype, coat color, and muzzle phenotype variationsin the Korean native brindle cattle (KNBC) maintaining family lines and to establish the mating system for increased brindle coat color appearance. KNBC with genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes, verified by PCR-RFLP, and brindle coat color appearance was determined. Fragments of the MC1R gene amplified by PCR were digested with MspI and RFLP was determined. KNBC had $E^+E^+$, $E^+e$, and ee genotypes. The $E^+e$ genotype was most common with 65%, compared to $E^+E^+$ (33.33%), or ee (1.67%). When the sire had $E^+e$ genotype and the dam had $E^+E^+$ genotype, and both of them had the whole body-brindle coat color, all of their offspring (4/4) had whole body-brindle coat color. When the sire had $E^+E^+$ genotype and the dam had $E^+e$ genotype, and both had whole body-brindle coat color, 44.44% (4/9) of the offspring had whole body-brindle coat color. The mating between the sires and dams with these two genotypes with whole body-brindle coat color may have the highest whole body-brindle coat color appearance in their offspring. Muzzle grades 3 or 4 were more common than other muzzle grades. This is the first report indicating the segregation of MC1R genotypes and the inheritance of coat color through family lines in KNBC. The mating system proposed from this study may increase the possibility of brindle coat color appearance in KNBC.

Failure of Ceramic Coatings Subjected to Thermal Cyclings (열피로에 의한 세라믹 코팅재의 파손)

  • Han Ji-Won
    • Journal of the Korean Society of Safety
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    • v.20 no.2 s.70
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    • pp.1-5
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    • 2005
  • An experimental study was conducted to develop an understanding of failure of ceramic coating when subjected to a thermal cycling. Number of cycles to failure were decreased as the coating thickness and the oxide of bond coat were increased. Using the finite element method, an analysis of stress distribution in ceramic coatings was performed. Radial compressive stress was increased in the top/bond coat interface with increasing coating thickness and oxide of bond coat.

Design and Development of a New Type of Doctor's Coat

  • Park, Judy Joohee
    • International Journal of Costume and Fashion
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    • v.12 no.2
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    • pp.53-65
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    • 2012
  • The scientific, professional and reliable image of doctor's coats is changing over the years, and many countries around the world are changing doctor's coat designs or removing them altogether. This study researches the history and significance of doctor's coats, and based on the research, designs and develops new doctor's coats for the Seoul National University Hospital Department of Dermatology where traditional white polyester and cotton coats are still being worn. The positive results of the study indicate that doctor's coat designs can be developed in different directions that reflect certain identities and moods while maintaining professionalism.

A Study on the Effects of the Thickness of Top Coat on the Thermal Stresses of a Sprayed Thermal Barrier Coating (용사 열차폐 코팅층의 두께가 열응력에 미치는 영향)

  • 김형남;양승한
    • Proceedings of the KWS Conference
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    • 2004.05a
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    • pp.223-225
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    • 2004
  • Based on the principle of complementary energy an analytical method is developed for determining thermal stress distribution in an thermal barrier coating. This method gives the stress distributions which satisfy the stress-free boundary conditions at the edge. Numerical examples are given in order to verify the method and to investigate the thickness effects of the ZrO$_2$-8wt%Y$_2$O$_3$ top coat on the integrity of thermal barrier coating consisted of IN738LC substrate and MCrAlY bond coat.

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Analysis and Detection of Coast Protein Gene of Barley Yellow Mosaic Virus and Barley Mield Mosaic Virus by RT-PCR (RP-PCR을 이용한 보리누른모자이크바이러스 (BaYMV)와 보리마일드모자이크바이러스(BaMMV)의 외피단백질 유전자 검정 및 해석)

  • 이귀재
    • Korean Journal Plant Pathology
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    • v.14 no.4
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    • pp.314-318
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    • 1998
  • Using the reverse transcription polymerase chain reaction (RT-PCR), a rapid and sensitive assay method for the detection and identification of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) was adapted. Two units of primers from each virus were selected and used for the determination of two different viruses. PCR fragments of BaYMV (ca. 0.9kb) and BaMMV (ca. 0.8kb) were obtained from the designed method for the assay of BaYMV and BaMMV coat protein. PT-PCR fragments were cloned using vector pT7 Blue and the sequences of the selected clones were analyzed. coat protein of BaYMV and that of BaMMV consisted of 297 amino acids (891 nucleotides) and 251 amino acids (753 nucleotides), respectively. The snalysis of coat protein genes from these two viruses showed that 45.6% of nucleotides sequence ad 34.9% of amino acid in BaYMV were homologous to those in BaMMV.

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Detection of 881A→881G Mutation in Tyrosinase Gene and Associations with the Black Ear Coat Color in Rabbits

  • Jiang, Y.L.;Fan, X.Z.;Lu, Z.X.;Tang, H.;Xu, J.-Q.;Du, L.-X.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.10
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    • pp.1395-1397
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    • 2002
  • The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

PVY Resistant Transgenic Potato Plants (cv Claustar) Expressing the Viral Coat Protein

  • Gargouri-Bouzid Radhia;Jaoua Leila;Mansour Riadh Ben;Hathat Yemna;Ayadi Malika;Ellouz Radhouane
    • Journal of Plant Biotechnology
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    • v.7 no.3
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    • pp.143-148
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    • 2005
  • The coat protein mediated resistance to potato virus Y is assessed here in transgenic potato plants (Solanum tuberosum L., cv Claustar). Therefore, the corresponding cDNA from tunisian isolate of the virus was cloned into Agrobacterium tumefaciens binary vector. The transgenic lines were subsequently analysed for the presence and expression of the transgene. The CP cDNA copy number was determined for kanamycin resistant plants. Three selected transgenic lines and their S1 progeny resulting from tuber germination showed a high protection level against the virus. These data appear to support the hypothesis that the virus resistance is mediated by the translated viral coat protein expressed in transgenic potato lines.

Complementary DNA Cloning and Nucleotide Sequence Analysis of Coat Protein Gene from TMV Tomato Strain (토마토에서 분리된 담배 모자이크 바이러스 외피단백질 유전자의 cDNA 클로닝 및 염기서열 분석)

  • 이청호;이영기;강신웅;박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.2
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    • pp.101-106
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    • 1996
  • Tobacco mosaic virus (TMV) tomato strain was isolated from tomato "Seo-Kwang" in Korea. The virion was purified by density gradient centrifugation, and total viral RNA was isolated from the purified particles. Coat protein (CP) cDNA of the virus was synthesized by RT-PCR, and the purified cDNA fragment was subcloned to pBluescript II SK-. The analysis of nucleotide sequence showed that this cDNA was 693 nucleotides long from the insert of clone p1571 and p1572 which contain complete codons of the viral coat protein gene (474 nucleotides) and 3' untranslated region. The nucleotides of coat protein encoding cDNA of the strain were 6 nucleotides less than that of TMV common strain isolated from tobacco plant in Korea. The CP gene showed 70% maximum homology with that of the common strain in the nucleotide level and 86% maximum homology in amino acid level.cid level.

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An Experimental Study on the Evaluation of Anti-corrosion Performance of High-frequency Arc Metal Spray film on Steel using EIS Method (전기화학적 임피던스 분광법(EIS)을 이용한 고주파 아크 금속용사 피막의 강재 방식성능 평가에 관한 실험적 연구)

  • Choe, Hong-Bok;Lee, Han-Seung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2014.11a
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    • pp.61-62
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    • 2014
  • To evaluate anti-corrosion performance of high-frequency arc metal spray film in accordance with metal type and epoxy sealing coat application status, electrochemical impedance spectroscopy(EIS) method was conducted in this study. As a result, in case of applying Al-Mg alloy metal spray film, it showed the best polarization resistance. In case of applying epoxy sealing coat, it showed increased polarization resistance of arc metal spray film. Through this experiment, it is judged that Al-Mg alloy arc metal spray film with epoxy sealing coat can increase the biggest anti-corrosion performance of high-frequency arc metal spray film on steel.

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A Study on the Dress in Nogultai(老乞大) (老乞大의 복식연구)

  • 김진구
    • The Research Journal of the Costume Culture
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    • v.4 no.1
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    • pp.1-14
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    • 1996
  • The purpose of the study is to analyze the dresses appeared in Nogultai. In the analysis of this study names and kinds of fabrics, colors, motifs, places of production and names of dresses were examined. In the study various kinds of fabric colors and motifs of fabrics were appeared in Nogulta. Also a variety of silks such s brocade, damask, plain silks, ra, silk gauze, hemp cloth, cotton cloth, wool and fur were found. Colors of fabrics were of blue, green, indigo blue, red, light blue, brown, yellow, black and white were described. Mostly cleouds, flowers and mixtures of floral designs were used in silk brocades, Walrus was motif the only animal revealed in silk brocade. Nanching, Hanchaw, Suchaw were famous production centers of silk damasks, silk gauge and silk ra. Also Shantung and Suchaw produced good quality plain silk. Various kinds of coats, waded coat, wadded short coat, inner jackets, vest trousers, winter cap were included. Also accessories such as belt, cap, boots, socks, money belt, pouch were described. In addition, cosmetics, cosmetic kits and names of semi precious stones were mentioned. Seasonal garments differed according to kinds of fabrics and materials used. Wadded coat, wadded short coat, vest, winter cap and wool socks appeared as winter wear.

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