• Microbiology and Biotechnology Letters
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• v.16 no.6
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• pp.494-498
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• 1988

The optimum conditions for glucoamylase production, and ethanol productivity of the transformant TSD-14 were investigated as compared with the parental strains. The properties of TSD-14 were comparatively similar to the donor S. diastaticus IFO 1046 as regards the conditions of glucoamylase production and ethanol productivity. The soluble starch was the most effective carbon source for the glucoamylase production. While inorganic nitrogen sources did not prompt cell growth and enzyme production, the organic nitrogen sources generally enhanced both cell growth and glucoamylase production. The metal salts such as FeSO$_4$, MgSO$_4$, MnCl$_2$, and NiSO$_4$were favorable to the enzyme production. And the optium temperature and initial pH for glucoamylase production were 3$0^{\circ}C$ and 5. The transformant TSD-14 produced 8.3%(v/v) ethanol from 15% sucrose medium, 4.8%(v/v) ethanol from 15% soluble starch medium, and 7.5%(v/v) ethanol from 15% liquefied potato starch medium. The corresponding fermentation efficiency were 84% , 45% and 70%, respectively.

• Journal of Astronomy and Space Sciences
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• v.20 no.1
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• pp.29-42
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• 2003

The KSR-3 magnetometers consist of the fluxgate magnetometer (MAG/AIM) for acquiring the rocket flight attitude information, and the search-coil magnetometer (MAG/SIM) for the observation of the Earth's magnetic fluctuations. The position (latitude, longitude, and height) and flight condition (the transformation angle) of the rocket is measured after the data based on these two magnetometers are compared with IGRF The gap in the vector of magnetic field between the position of the launching point and an impact point is taken into account in data reduction. Angular variation of pitch, yaw, and roll can be researched when the data is applied to the coordinate system of the rocket.

• Journal of Plant Biotechnology
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• v.32 no.3
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• pp.209-215
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• 2005

Lactoferrin is an iron-binding glycoprotein with many biological roles, including the protection against microbial and virus infection, stimulation of the immune system. We developed the transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing the human lactoferrin (hLf) protein following Agrobacterium tumefaciens-mediated transformation. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to hLf cDNA under the control of an oxidative stress-inducible SWPA2 promoter was engineered. Transgenic Siberian ginseng cultured cells to produce a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analysis. ELISA and western blot analysis showed that full length-hLf protein was synthesized in the transgenic cells. The production of hLf increased proportionally to cell growth and reached a maximal (up to 3% of total soluble proteins) at the stationary phase. These results suggest that the transgenic Siberian ginseng cultured cells in this study will be biotechnologically useful for the commercial production of medicinal plant cell cultures to produce hLf protein.

• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.375-380
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• 2007

This study was conducted to develop herbicide-resistance domestic maize plants by introducing the CP4 5-enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) gene using Agrobacterium tumefaciens-mediated immature embryo transformation. Immature embryos of five genotypes (HW1, KL103, HW3, HW4, HW7) were co-cultivated with strains Agrobacterium tumefaciens (strain C58C1) containing the binary vector (pCAMBIA2300) carrying Ubiquitin promoter-CP4 EPSPS gene and Cauliflower mosaic virus 35S (CaMV35S) promoter-nptll gene conferring resistance to paromomycin as a selective agent. The presence and expression of CP4 EPSPS transgene were confirmed by PCR, RT-PCR and Northern blot analysis, respectively. Also, the resistance to glyphosate in the transgenic maize ($T_1$) was analyzed by shikimate accumulation assay. The frequency (%) of paromomycin-resistance callus was 0.37, 0.03, 2.20, 2.37, and 0.81% in pure lines HW1, KL103, HW3, HW4 and HW7, respectively. EPSP transgene sequences were amplified in putative transgenic plants that regenerated from paromomycin-resistance calli of two inbred lines (HW3, HW4). Of them, RT-PCR and Northern blot analyses revealed that the transgene was only expressed in two transgenic events (M266, M104) of HW4 inbred line, and a mild glyphosate resistance of transgenic event (M266) was confirmed by the lower shikimate accumulation in leaf segments. These results demonstrate that transgenic maize with herbicide-resistance traits in Korean genotype can be genetically obtained.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.7
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• pp.655-663
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• 2017

With the development of ICT technology, the indoor utilization of robots is increasing. Research on transportation, cleaning, guidance robots, etc., that can be used now or increase the scope of future use will be advanced. To facilitate the use of mobile robots in indoor spaces, the problem of self-location recognition is an important research area to be addressed. If an unexpected collision occurs during the motion of a mobile robot, the position of the mobile robot deviates from the initially planned navigation path. In this case, the mobile robot needs a robust controller that enables the mobile robot to accurately navigate toward the goal. This research tries to address the issues related to self-location of the mobile robot. A robust position recognition system was implemented; the system estimates the position of the mobile robot using a combination of encoder information of the mobile robot and the absolute space coordinate transformation information obtained from external video sources such as a large number of CCTVs installed in the room. Furthermore, vector field histogram method of the pass traveling algorithm of the mobile robot system was applied, and the results of the research were confirmed after conducting experiments.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.4
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• pp.351-357
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• 1987

A laboratory experiment was conducted to find out the effect of organic fertilizer as Bio-com$^{(R)}$ on the changes of pH and Eh values, gas evolution, ammonification and nitrification, and microbial population with farmer's compost and refused mushroom compost in submerged paddy condition. The results obtained are summarized as follows: 1. Application of compost and refused mushroom compost was increased the pH values than that of NPK alone. Organic fertilizer of Bio-com$^{(R)}$ showed the same results of the farmer's compost or refused mushroom compost. 2. Population of soil microbes as bacteria, actinomycetes and fungi was increased by application of compost, refused mushroom compost and Bio-com$^{(R)}$. Moreover, the results were pronounced more with the addition of NPK. 3. The application of Bio-com$^{(R)}$ was effected to the increase of the amount of $NO_3-N$ and the rate of nitrification than NPK, farmer's compost or refused mushroom compost. 4. The amounts of evolved gases as $CH_4$, $CO_2$, and $N_2O$ were not much differed with application of kinds of compost and NPK, but little increasing tendency was observed in application of NPK than that of NPK+kinds of compost.

• Journal of the Korean Geotechnical Society
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• v.28 no.4
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• pp.43-53
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• 2012

There are many factors that can cause problems in the application of the lightweight air-trapped soil in the field. Temperature is one of them. However, the effect of temperature on the characteristics of lightweight air-trapped soil has not been studied. Thus, this experimental study is conducted. This study considers three different unit weights of lightweight air-trapped soils, 6, 8, and 10 kN/$m^3$ which are mostly applied to the field. The lightweight air-trapped soil specimens are cured in the condition of different temperatures based on the characteristic of temperature of Korea. The unconfined compression tests are conducted on the prepared specimens. The settlement behavior of lightweight air-trapped soil on temperature is observed. A photograph is taken using SEM to determine the transformation of air-foams inside the lightweight air-trapped soil on curing temperature. As the results, the effect of curing temperature on the characteristics of lightweight air-trapped soil including compressive strength, settlement, and formation of air-foams is confirmed. Finally, based on this study, the allowable curing temperature of $8^{\circ}C{\sim}25^{\circ}C$ which can guarantee the quality of lightweight air-trapped soil is proposed.

• Journal of Korean Clinical Health Science
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• v.8 no.1
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• pp.1362-1368
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• 2020

Purpose. In this study, The change of the accommodation function due to the use of a smartphone was objectively evaluated using an Auto Refractokeratometer and was to investigate the effect of accommodation response and microfluctuations of accommodation on stable fatigue. Methods. Twenty subjects (20 males, 20 females) who had no abnormalities in ophthalmic disease and strabismus, diplopia, suppression, convergence and ocular movement, and corrected visual acuity and unaided visual acuity of 0.8 or more were performed. The average age of the participants is (21.78 ± 1.78) years old. The refractive power of the eye was measured using an Auto Refractokeratometer (Speedy-i K-model, Righton Mfg Co. Tokyo Japan), and the equivalent spherical power was automatically calculated from the measured refractive power. The accommodation response amount was calculated for the accommodation stimulation amount in 8 steps in 0.5D increments from + 0.50D to -3.00D in the calculated equivalent spherical power. The microfluctuations of accommodation in the high frequency region was calculated according to the change in the amount of the accommodation stimulus. At this time, the spectral power of the microfluctuations of accommodation was analyzed by Fast Fourier Transformation (FFT). Results. After using the smartphone, it was found that the accommodation response to accommodation stimulation decreased and the microfluctuations of accommodation increased. Conclusions. It can be said that the use of a smartphone affects the accommodation response and the microfluctuations of accommodation, thereby causing a accommodative stable fatigue. Therefore, it is thought that the use of a smartphone for a long time may bring about a change in the accommodation function.

• Journal of IKEEE
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• v.21 no.4
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• pp.412-415
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• 2017

In this paper, we propose a multi license plate recognition system using high resolution $360^{\circ}$ omnidirectional IP camera. The proposed system consists of a planar division part of $360^{\circ}$ circular image and a multi license plate recognition part. The planar division part of the $360^{\circ}$ circular image are divided into a planar image with enhanced image quality through processes such as circular image acquisition, circular image segmentation, conversion to plane image, pixel correction using color interpolation, color correction and edge correction in a high resolution $360^{\circ}$ omnidirectional IP Camera. Multi license plate recognition part is through the multi-plate extraction candidate region, a multi-plate candidate area normalized and restore, multiple license plate number, character recognition using a neural network in the process of recognizing a multi-planar imaging plates. In order to evaluate the multi license plate recognition system using the proposed high resolution $360^{\circ}$ omnidirectional IP camera, we experimented with a specialist in the operation of intelligent parking control system, and 97.8% of high plate recognition rate was confirmed.

• Korean Journal of Plant Resources
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• v.27 no.1
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• pp.51-59
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• 2014

In this study, we developed a high frequency watermelon regeneration system using three breeding lines ('B02', 'B05' and 'D04') of watermelon (Citrullus lanatus (thunb.) Matsum. & Nakai) which are differed in their fruits in shape, color of pericarp and flesh. The highest frequency of explants with callus was observed by using explants that consist of cotyledon proximal part end in all breeding lines, and the highest rate of callus induction was obtained on MS medium containing 1.0 mg/L BAP + 0.5 mg/L IAA for 'B02' (94%), 3.0 mg/L BAP for 'B05' (95%), 3.0 mg/L BAP + 0.1 mg/L IAA for 'D04' (90%). The highest shoot regeneration rates from derived callus were obtained on MS medium containing 1.0 mg/L BAP + 0.5 mg/L IAA for 'B05' (94%), and then a 'B02' (81%) with a same culture conditions, and the lowest regeneration was obtained on MS medium containing 1.0 mg/L BAP for 'D04' (56%). Regenerated plants showed the best rates of root formation on MS containing 0.1 mg/L IBA for 'B02' (67%), 0.1 mg/L NAA for 'B05' (87%), 0.5 mg/L IAA for 'D04' (88%). The regenerated plants showed a 100% survival rate in soil condition. The tissue culture and regeneration conditions obtained from this study will be useful for regenerating plants in breeding applications, and will be a useful tool for further genetic transformation studies on watermelons.