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http://dx.doi.org/10.5010/JPB.2005.32.3.209

Transgenic Siberian Ginseng Cultured Cells That Produce High Levels of Human Lactoferrin  

Jo Seung-Hyun (Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KIRBB), Department Food Science and Technology, Chungnam National University)
Kwon Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KIRBB))
Kim Jae-Whune (Microplants Co., LTD)
Lee Ki-Teak (Department Food Science and Technology, Chungnam National University)
Kwak Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KIRBB))
Lee Haeng-Soon (Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KIRBB))
Publication Information
Journal of Plant Biotechnology / v.32, no.3, 2005 , pp. 209-215 More about this Journal
Abstract
Lactoferrin is an iron-binding glycoprotein with many biological roles, including the protection against microbial and virus infection, stimulation of the immune system. We developed the transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing the human lactoferrin (hLf) protein following Agrobacterium tumefaciens-mediated transformation. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to hLf cDNA under the control of an oxidative stress-inducible SWPA2 promoter was engineered. Transgenic Siberian ginseng cultured cells to produce a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analysis. ELISA and western blot analysis showed that full length-hLf protein was synthesized in the transgenic cells. The production of hLf increased proportionally to cell growth and reached a maximal (up to 3% of total soluble proteins) at the stationary phase. These results suggest that the transgenic Siberian ginseng cultured cells in this study will be biotechnologically useful for the commercial production of medicinal plant cell cultures to produce hLf protein.
Keywords
Acanthopanax senticosus; high expression promoter; human lactoferrin; recombinant protein; transgenic cell cultures;
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