• BMB Reports
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• 제49권2호
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• pp.116-121
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• 2016

Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.

• 디지털융복합연구
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• 제9권5호
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• pp.193-202
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• 2011

본 연구는 인천소재 국가 산업단지인 남동공단에 위치한 기업들의 클러스터 형성과 관련된 구성 요소와, 특성, 이들 특성요인이 클러스터에 분포한 기업성과에 미치는 영향을 분석하여 향후 혁신 클러스터 구축방향에 대한 대안제시를 목적으로 한다. 연구결과 성공한 클러스터 기업들의 특징은 클러스터 집약정도, 네트워킹의 정도, 집단학습, 혁신시너지, 국지화 등이 유의한 차이를 나타내는 것으로 나타났다. 또한 대기업과 중소기업간의 협력관계가 중요한 성패요인이며 이를 위해 공동연구개발이나 프로그램운영 등에 지원이 필요하다.

• 반도체디스플레이기술학회지
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• 제19권4호
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• pp.1-5
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• 2020

By adapting black body leadflame and thin film type Green A filter, we successfully demonstrated night vision imaging system (NVIS) Green A compatible LED. Fabricated NVIS compatible LEDs show small form factor compared to that of commercialized NVIS compatible LED. Especially, NVIS radiance and chromaticity of MIL-STD-3009 specification can be satisfied simultaneously and easily by controlling the color temperature of the white LED as well as the concentration of the Green A dye and the thickness of the Green A filter. The optimal dye concentration of the NVIS Green A filter is expected to be about 1 wt%. The results of this study are expected to contribute to miniaturization, weight reduction and localization of avionic display and lighting devices.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.45-45
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• 2003

We isolated a novel ankyrin-repeat containing protein, rSIAP (rSlo Interacting Ankyrin-repeat Protein), as an interacting protein to the cytosolic domain of the alpha-subunit of rat large-conductance Ca$\^$2+/-activated K$\^$+/ channel (rSlo) by yeast two-hybrid screening. Affinity pull-down assay showed the direct and specific interaction between rSIAP and rSlo domain. The channel-binding proteins can be classified into several categories according to their functional effects on the channel proteins, i.e. signaling adaptors, scaffolding net, molecular tuners, molecular chaperones, etc. To obtain initial clues on its functional roles, we investigated the cellular localization of rSIAP using immunofluorescent staining. The results showed the possible co-localization of rSlo and rSIAP protein near the plasma membrane, when co-expressed in CHO cells. We then investigated the functional effects of rSIAP on the rSlo channel using electrophysiological means. The co-expression of rSIAP accelerated the activation of rSlo channel. These effects were initiated at the micromolar [Ca$\^$2+/]$\_$i/ and gradually increased as [Ca$\^$2+/]$\_$i/ raised. Interestingly, rSIAP decreased the inactivation kinetics of rSlo channel at micromolar [Ca$\^$2+/]$\_$i/, while the rate was accelerated at sub-micromolar [Ca$\^$2+/]$\_$i/. These results suggest that rSIAP may modulate the activity of native BK$\_$Ca/ channel by altering its gating kinetics depending on [Ca$\^$2+/]$\_$i/. To localize critical regions involved in protein-protein interaction between rSlo and rSIAP, a series of sub-domain constructs were generated. We are currently investigating sub-domain interaction using both of yeast two-hybrid method and in vitro binding assay.

• ETRI Journal
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• 제29권2호
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• pp.189-200
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• 2007

This paper addresses the localization and navigation problem in the movement of service robots by using invisible two dimensional barcodes on the floor. Compared with other methods using natural or artificial landmarks, the proposed localization method has great advantages in cost and appearance since the location of the robot is perfectly known using the barcode information after mapping is finished. We also propose a navigation algorithm which uses a topological structure. For the topological information, we define nodes and edges which are suitable for indoor navigation, especially for large area having multiple rooms, many walls, and many static obstacles. The proposed algorithm also has the advantage that errors which occur in each node are mutually independent and can be compensated exactly after some navigation using barcodes. Simulation and experimental results were performed to verify the algorithm in the barcode environment, showing excellent performance results. After mapping, it is also possible to solve the kidnapped robot problem and to generate paths using topological information.

• Journal of Periodontal and Implant Science
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• 제24권3호
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• pp.607-617
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• 1994

To determine the effect of tenascin on forming periodontal pocket and pseudopocket, the ginival tissues were surgically obtained from the patients with adult periodontitis(10) and non-inflammatory phenytoin-associated gingival hyperplasia(5). The excised tissue specimens were fixed in neutral formalin for $6{\sim}24$ hours, embedded with paraffin, sectioned at 4-6m in thickness, mounted on glass slides coated with 3-aminopropyltriethoxysilane(Sigma Chemical Co., St. Louis, MO, U.SA.) and immunohistochemically processed by Avidin-Biotin peroxidase complex method for the localization of tenascin, using monoclonal mouse anti-human tenascin antiboday(Chemicon-International Inc., Temecula, CA, U.S.A., 1: 5,000) as the primary antibody. Regardless of periodontal pocket and pseudopocket, tenascin was localized along the connective tissue subjacent to basement membrane of gingival epithelium, and strong positive reactivity was obviously noted in the papillary projections of gingival connective tissue. The results suggest that tenascin may affect the development of papillary projections and the proliferation of epithelial cells.

• 한국항행학회논문지
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• 제14권2호
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• pp.206-212
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• 2010

본 논문에서는 유비쿼터스 환경에서 로봇의 응용서비스를 제공하는데 필요한 네트워크로서 로보틱 지그비 네트워크의 개념을 소개하고 이를 이용한 응용 시나리오를 제시한다. 제시한 응용시나리오의 기본이 되는 네트워크 연결 및 데이터 전송에 관한 실험을 수행하였다. 이 실험 결과를 통해 앞으로 Robotic Zigbee Network를 이용한 위치인식 오차율을 최소화하는 로봇의 위치추정 및 추적 알고리즘 개발의 기반을 마련한다.

• 한국지능시스템학회논문지
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• 제26권2호
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• pp.160-167
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• 2016

본 논문은 천장 영상 정보를 이용한 이동 로봇 위치추정 방법을 제안한다. 지도상에 천장 영상의 랜드 마크의 위치는 미리 알고 있지만, 지도상의 랜드 마크와 감지된 랜드 마크 사이의 대응관계 정보는 주어지지 않는다. 단지, 로봇의 이동 시작 단계에서 랜드 마크들에 대한 상대적인 로봇의 위치가 주어진다. 로봇의 위치 및 천장 영상에서 감지된 특징점의 ID를 찾기 위해 파티클 필터 방법을 이용한다. 제안한 방법을 천장에 동일한 형태의 원형 랜드 마크를 가진 실내 환경에서 실험하여 성능을 검증하였다. 본 논문에서 제안한 위치추정 방법은 레이저 영역 센서에 의해 측정된 벽까지의 거리 또는 RF 나 초음파에 의해 측정된 비이컨까지의 거리 값에 큰 불확실성이 존재하는 물류 창고와 같은 환경에서 사용하기에 적합하다.

• Animal cells and systems
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• 제11권1호
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• pp.39-50
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• 2007

Tau plays a role in numerous neuronal processes, such as vesicle transport, microtubule-plasma membrane interaction and intracellular localization of proteins. SUMO (Small Ubiquitin-like Modifier) modification (SUMOylation) appears to regulate diverse cellular processes including nuclear transport, signal transduction, apoptosis, autophagy, cell cycle control, ubiquitin-dependent degradation, as well as gene transcription. We noticed that putative SUMOylation site is localized at $^{340}K$ of $Tau(^{339}VKSE^{342})$ with the consensus sequence information (${\Phi}KxE$ ; where ${\Phi}$ represents L, I, V or F and x is any amino acid). In this report, we demonstrated that $^{340}K$ of Tau is the SUMOylation site and that a point mutant of Tau S214E (an analog of the phospho $^{214}S$ Tau) promotes its SUMOylation at $^{340}K$ and its nuclear or nuclear vicinity localization, by co-immunoprecipitation and confocal microscopy analysis. Further, we demonstrate that the Tau S214E (neither Tau S214A nor Tau K340R) mutant increases its protein stability. However, the SUMOylation at $^{340}K$ of Tau did not influence cell survival, as determined by FACS analysis. Therefore, our results suggested that the phosphorylation of Tau on $^{214}S$ residue promotes its SUMOylation on $^{340}K$ residue and nuclear vicinity localization, and increases its stability, without influencing cell survival.

• 지질공학
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• 제25권1호
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• pp.103-113
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• 2015

본 연구에서는 신호 대 잡음비가 낮은 미소파괴음 신호에 대한 진원 위치표정 결과의 정확성 향상을 위하여 가변창문함수를 이용한 P파 도달시간 결정 알고리즘을 제안하였다. 제안된 알고리즘은 기존 P파 도달시간 결정알고리즘과 함께 신호 대 잡음비(SNR)가 각기 다른 가상의 미소파괴음 신호에 적용하여 정확성을 검토하였다. 실험결과 기존 P파 도달시간 결정알고리즘은 SNR이 낮아질수록 결과의 정확성이 낮아지며 특히 SNR이 2.14에서는 모든 알고리즘에서 오류가 발생하였다. 반면에, 제안된 알고리즘은 신호의 분산을 고려한 가변창문함수를 신호에 적용하기 때문에 신호의 주파수특성과 신호의 증폭을 동시에 고려하였다. 따라서 SNR 2.14 이하의 신호에서도 제시된 알고리즘은 정확한 결과를 제시하였다.