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http://dx.doi.org/10.5483/BMBRep.2016.49.2.232

Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle  

Lee, Hyun (Division of Life Sciences, Korea University)
Kim, Seung-Hyeob (Division of Life Sciences, Korea University)
Lee, Jae-Seon (Department of Biomedical Sciences, College of Medicine, Inha University)
Yang, Yun-Hee (Department of Chemistry, Seoul National University)
Nam, Jwa-Min (Department of Chemistry, Seoul National University)
Kim, Bong-Woo (Division of Life Sciences, Korea University)
Ko, Young-Gyu (Division of Life Sciences, Korea University)
Publication Information
BMB Reports / v.49, no.2, 2016 , pp. 116-121 More about this Journal
Abstract
Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.
Keywords
Cytochrome c; Extracellular NADH-dependent respiration; Oxidative phosphorylation complexes; Sarcolemma; Skeletal muscle;
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