• 한국식품영양과학회지
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• 제40권3호
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• pp.385-392
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• 2011

Glyceraldehyde를 이용하여 제조된 AGEs 화합물(glycer-AGEs)을 단구세포인 THP-1, 혈관내피세포인 HUVEC 및 이 두 세포가 동시에 배양된 system에서 100 ${\mu}g/mL$로 처리 후 24시간까지 처리시간을 달리하여 처리하였다. 배양시간 동안 각 세포와 배양액을 회수하여 TNF-$\alpha$와 IL-1$\beta$의 발현을 mRNA 수준에서 조사하였다. 그 결과, THP-1의 경우 배양 2시간에서 TNF-$\alpha$나 IL-1$\beta$의 mRNA 발현이 대조구에 비해 증가되었으나 혈관내피세포인 HUVEC의 경우에는 24시간 배양하는 동안 유의적인 차이가 없었다. 그러나 두세포를 동시에 배양한 system에서 혈관내피세포인 HUVEC의 경우에는 배양 4시간에서 대조구보다 TNF-$\alpha$의 발현은 4.4배, IL-1$\beta$의 경우 5.5배 정도 증가되는 것을 확인할 수 있었다. TNF-$\alpha$와 IL-1$\beta$의 배지 내에서의 농도를 측정해 본 결과, THP-1만 배양한 경우 대조구의 배지 내 TNF-$\alpha$ 함량이 배양 6시간에 98.2 pg/mL로 대조구 53.8 pg/mL보다 증가하였으며 HUVEC의 경우 배양 8시간에 93.3 pg/mL로 증가하였다. 그러나 co-culture의 경우 배양 4시간부터 증가하여 배양 8시간에 199.2 pg/mL로 증가하였다. RAGE는 TNF-$\alpha$와 IL-1$\beta$의 발현 pattern과 다르게 단독 및 co-culture에서 배양 16시간에 대조구에 비해 각각 1.6배, 24시간에 1.9배 증가하였다. 따라서 본 연구 결과 최종당화산물에 의해 혈관내피세포의 기능상실의 연구에 있어 co-culture조건이 유용하며, 특히 mRNA 수준에서는 4시간에, protein 수준에서는 8시간에 효능을 측정하면 유효성 있는 연구 성과를 얻을 있을 것으로 예측할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.161-170
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• 2017

Meningitis caused by Streptococcus suis serotype 2 (S. suis 2) is a great threat to the pig industry and human health. Virulence factors associated with the pathogenesis of meningitis have yet to be clearly defined, even though many potential S. suis 2 virulence factors have been identified. This greatly hinders the progress of S. suis 2 meningitis pathogenesis research. In this study, a co-culture blood-brain barrier (BBB) model was established using primary porcine brain microvascular endothelial cells and astrocytes, and the whole genome library of S. suis 2 was constructed using phage display technology. Finally, a total of 14 potential virulence factors contributing to S. suis 2 adherence to and invasion of the BBB were selected by analyzing the interactions between the phage library and the co-culture model. Twelve of these factors have not been previously reported in meningitis-related research. The data provide valuable insight into the pathogenesis of S. suis 2 meningitis and potential targets for the development of drug therapies.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.295-298
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• 2011

Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.

• International Journal of Advanced Culture Technology
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• 제8권1호
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• pp.44-55
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• 2020

The degree of mutual understanding between nursing students and instructors regarding simulation-based education remains unknown. The purpose of this study was to identify the subjectivity of nursing students and instructors about simulation-based learning, and was intended to expand the mutual understand by employing the co-orientation model. Q-methodology was used to identify the perspectives of 46 nursing students and 38 instructors. Perception types found among students in relation to simulation-based learning were developmental training seekers, instructor-dependent seekers, and learning achievement seekers. The instructors estimated the student perception types as passive and dependent, positive commitment, demanding role as facilitators, and psychological burden. Perception types found among instructors included nursing capacity enhancement seekers, self-reflection seekers, and reality seekers. The students classified the instructors' perception types as nursing competency seekers, learning reinforcement seekers, and debriefing-oriented seekers. As a result of the analysis of these relations in the co-orientation model, instructors identified psychological burden and passive and dependent cognitive frameworks among students; however, these were not reported in the students' perspectives. Likewise, the reality seekers type found among the perception types of instructors was not identified by the students. These findings can help develop and implement simulation-based curricula aimed at maximizing the learning effect of nursing students.

• International Journal of Advanced Culture Technology
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• 제7권4호
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• pp.321-326
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• 2019

As AI technology is recently introduced into various fields, it is being applied to the fashion field. This paper proposes a system for recommending cody clothes suitable for a user's selected clothes. The proposed system consists of user app, cody recommendation module, and server interworking of each module and managing database data. Cody recommendation system classifies clothing images into 80 categories composed of feature combinations, selects multiple representative reference images for each category, and selects 3 full body cordy images for each representative reference image. Cody images of the representative reference image were determined by analyzing the user's preference using Google survey app. The proposed algorithm classifies categories the clothing image selected by the user into a category, recognizes the most similar image among the classification category reference images, and transmits the linked cody images to the user's app. The proposed system uses the ResNet-50 model to categorize the input image and measures similarity using ORB and HOG features to select a reference image in the category. We test the proposed algorithm in the Android app, and the result shows that the recommended system runs well.

• International Journal of Advanced Culture Technology
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• 제9권1호
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• pp.129-135
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• 2021

In this study, we built a mock-up of an indoor garden for private use and vertical gardens were installed on the walls of this indoor garden model. The purpose of this study is to examine the types of plants for best fit for growth and nurture in vertical garden and to identify the effects of indoor air quality improvement by these plants. As the result of the experiment, 22 species out of 32 species previously used for indoor garden was selected to be suitable for vertical gardens of a personal indoor garden. 10 species were found to be inappropriate for a personal indoor garden in terms of ornamental value, growth status and maintenance. The effect of plants on reducing CO2 has been proven by many studies. Also, through photosynthesis, plants combine CO2 with water and produce sugars and O2 (oxygen). Everyone accepts this fact. In nature, the production of oxygen is so important that without plants we would soon use it up and die. From the NASA Fact Sheet we know that air contains 20.95% O2 and 0.04% CO2. If you had enough plants in a room to use up all of the all of CO2 and convert it to oxygen, the oxygen levels would increase from 20.95% to 21%. This increase is difficult to detect and would have no effect on humans.

• 복식문화연구
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• 제32권2호
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• pp.148-163
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• 2024

With the digitalization of production and consumption environments, consumers are no longer merely targets of marketing, but key players in creating value jointly with companies by participating in various decision-making processes. Much virtual content in particular, such as fashion shows, exhibitions, games, social activities, and shopping, which fashion brands implement in virtual worlds, cannot be completed without consumers' active engagement and interaction. Thus, this study considers consumers' participation in virtual content provided by fashion brands as value co-creation in virtual worlds. This study aims to examine how consumer (i.e., consumer smartness) and fashion firm (i.e., perceived intellectual capital) factors influence value co-creation behavior intention in virtual worlds. Data were collected from 410 consumers in their 20s nationwide through an online survey, and a higher-order structural equation modeling analysis was conducted to test the research model. The results showed that both consumer smartness and perceived intellectual capital positively influenced customer participation behavior and citizenship behavior intentions. Specifically, perceived intellectual capital had a greater impact on value co-creation behavior in the virtual world than consumer smartness. The findings provide empirical evidence that the fashion firms' intangible assets and consumers' competence in the digital shopping environment encourage their intentions to co-create value in virtual worlds.

• KSBB Journal
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• 제15권6호
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• pp.644-648
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• 2000

Biological fixation of carbon dioxide using microalgae is known as an effective CO$_2$reduction technology. However, many environmental factors influence microalgal productivity. Optimal cultivation factors were determined for the green alga, Euglena gracilis Z, which offers high protein and vitamin E content for animal fodder. In batch culture in a photovioreactor, it was found that theinitial pH, temperature, CO$_2$concentration in air, and light intensity during the optimal cultivating conditions were 3.5, 27$^{\circ}C$, 5-10% and 520 ${\mu}$mol/㎡/s, respectively. When tap water and freshwater were used as cultivating media unsterilized tap water was found to be effective. A kinetic model was considered to determine the relationship between the specific growth rate and the light intensity. The half-velocity coefficient (K(sub)I) in the Monod model under photoautotrophic conditions was 978.9 ${\mu}$mol/㎡/s.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.698-699
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• 2003

DA-3711 is a novel, anti wrinkle agent containing growth factors derived from human skin culture. In this study, effect of topical application of DA-3711 on chronic ultraviolet B (UVB)-induced skin damage was evaluated in hairless mice model. Exposure to UVB for 8 weeks induced apparent wrinkles on the back skin of the mice. The dorsal surfaces were exposed to UVB for a further 8 weeks, during which the surfaces where treated daily by topically application of a lotion containing either 35％ or 70％ of DA-3711. For comparison, lotion base, lotion base containing Cylasphere Retinol(equation omitted) (2500 I.U., Coletica) and NouriCe $l^{(R)}$ (Biozhem) were applied topically once a day for 8 weeks.(omitted))

• 복식문화연구
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• 제24권4호
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• pp.498-510
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• 2016

As social media are emerging as essential communication channels for corporates in all areas, luxury jewelry brands have implemented enhanced strategies for brand story videos by creating brand channels on YouTube. In this study, a comparative analysis of the videos made available by Cartier and Tiffany&Co. on their YouTube Brand Channels was conducted, with the aim of identifying their strategic characteristics. The research method encompassed, both literature review and empirical investigations. A quantitative analysis was conducted by means of the 'HEART' model, a type of luxury jewelry brand story, and the following common strategic elements were identified. First, in their brand story videos both brands focus primarily on 'theme'. Second, 'relationship' and 'artisanship' are emphasized in addition to 'theme'. Third, the videos incorporate high level aesthetics to stimulate the fantasies and dreams of their audiences. The strategic differences between the brands are as follows. : First, 'artisanship' is given the greatest significance along with 'theme' in Cartier videos, while 'relationship' has prime significance in Tiffany&Co. videos. Second, a difference between a European approach and an American approach towards luxury brand building is evident, with Cartier hosting exhibitions while Tiffany&Co. provides gala videos instead.