• 한국자원식물학회지
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• 제19권3호
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• pp.381-384
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• 2006

This study was conducted to selected Korean black raspberry (Rubus coreanus Miq.) for high productivity. The eight major agronomic traits were investigated from 198 clones of the clone bank established in Korea Forest Research Institute, Suwon, Korea. The selection levels based on number of fruit per fructify lateral (NFFL) over 20, and fruit weight (FW) over 1.3g, and yield of individual per fructify lateral (YIFL) over 25g, were applied on 198 clones, resulted in 17 clones selected. The selected superior trees, 17 clones, appeared regional differences for amount of fruiting among 4 different test sites. When number of fruit per fruit petiole (NRFP), fruit weight (FW), yield of individual (YI) and sugar content were satisfied over 20, 1.4g, 6kg and 9.5 brix, respectively, as a select condition, 5 clones were reselected as the superior trees among 17 clones. for 3 years.

• 한국자원식물학회지
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• 제19권3호
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• pp.440-446
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• 2006

To investigate flowering related genes in winter-type oilseed rape (Brassica napus L. cv. Tammi), differentially expressed genes were isolated from leaves of the plant after low temperature treatment which is requirements for floral induction. As a result of suppression subtractive hybridization (SSH), 288 clones were randomly selected from SSH library. Using reverse Northern blot analysis, 150 of 288 clones were identified to be differentially expressed. Out of these 150 clones, 45 clones showed very high identities with the known genes. Four clones showed very high identities over 90% with metallothionein-like gene that is related to flowering-induced genes. Of these 4 clones, the cDNA clone, rfs-13, revealed high identity with meotallothionein-like protein in Arabidopsis thaliana (98%) and Brassica compestris (89%). Furthermore, gene expressed in immature flower stages was confirmed by Northern blot analysis.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.316-327
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• 2007

In this study, a Korean native cattle strain (Hanwoo) evidencing high performance in terms of both meat quality and quantity was employed in the generation of 150,000 BAC clones with an average insert size of 140 kb, and corresponding to about a 6X coverage of bovine chromosomal DNA. The BAC clones were pooled in a mini-scale via three rounds of a pooling protocol, and the efficiency of this pooling protocol was evaluated by testing the accuracy of accessibility to the positive clones, via a PCR-based screening method. Two sets of primers designed from each of two known genes were tested, and each yielded 2 or 3 positive clones for each gene, thereby indicating that the BAC library pooling system was appropriate with regard to the accession of the target BAC clones. Analyses of $3.3{\times}10^6$ base pairs obtained from the 7,090 BAC end sequence (BES) showed that 34.88% of the DNA sequence harbored the repetition sequence. Analysis of the 7,090 BES to the $1^{st}$ and $2^{nd}$ generation radiation hybrid map of the cattle genome, using the COMPASS program designed for the construction of a cattle-human comparative mapping, resulted in the localization of a total of 1,374 clones proximal to 339 $1^{st}$ generation markers, and 1,721 clones proximal to 664 $2^{nd}$ generation markers. Collectively, the BAC library and pooling system of the BAC clones from the Korean cattle, coupled with the chromosome-localized BAC clones, will provide us with novel tools for the excavation of desired clones for genome mapping and sequencing, and will also furnish us with additional information regarding breed differences in cattle.

• 임산에너지
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• 제1권2호
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• pp.1-19
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• 1981

In order to investigate biomass yield of one-year-old hyblrid poplars, Pripulusalba x P. glandulosa $F_1$ and Populus nigra var. italica x P. maximowiczii $F_1$clones, as energy and fiber resources, dry matter yield, leaf area, leaf area index, dry matter production ability, specific gravity and fiber length and width were measured. Dry matter yield was 1.89 ton/ha for Poplus alba x P. glandulosa $F_1$ and 3.63 ton/ha for Populus nigra via.italica x P. merximowiczii $F_1$ clones in the planting density of 20,000 trees/ha and in the planting density of 40,000 trees/ha was 3.87 ton/ha for Populus alba x P. glandulosa $F_1$and 5.64 ton/ha for Populus nigra var. italica x P. maximowiczii $F_1$ clones. Leaf area index was 1.24mtim2 in the planting density of 20,000 trees/ha and 2.45 m31m3 in the density of 40,000 trees/ha for Populus alba x P. glandulosa $F_1$ clones and it was 1.96 m21m2 in the planting density of 20,000 trees/ha and 3.36 m21m2 in the density of 40,000 trees/ha for the hybrid $F_1$ Populus nigra var. italica x P. maximowiczii clones. The average specific gravity of the hybrid poplars was 0..36 when bark and pith were included and 0.31 when bark and pith were removed in the plot of 20,000 trees/ha and in the 40,000 trees/ha plot showed 0.35 and 0.31 respectively, for Populus alba x P. glandulosa $F_1$clones. It was 0.36 when bark and pith were included and 0.32 when bark and pith were removed in the 20,000 trees/ha plot and in the 40,000 trees/ha plot was 0.34 and 0.31 respectively for Populus nigra var. italica x P. maximowiczii $F_1$clones. The average fiber length was 0.57 mm in the 20,000 trees/ha plot and 0.58 mm in the 40,000 trees/ha plot for Poplus alba x P. glandulosa $F_1$clones and was 0.60 in both plots of 20,000 trees/ha and 40,000 trees/ha for Populus nigra var. italica x P. maximowiczii $F_1$ clones. There is a big clonal variation among those clones studied, showing high selection potential in both species.

• ALGAE
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• 제19권2호
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• pp.145-148
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• 2004

As a result of the 2-year monthly monitoring of the phytoplankton community at 3 stations in Mankyeong Estuary, Korea, we learned that cyan bacterial species of the genus Anabaena occurred at most sampling points with huge salinity differences (0.1-32.5 psu). We isolated several clones of Anabaena spp. from the monitoring stations, and screen out two euryhaline and nitrogen-fixing Anabaena clones, CB-MAL21 and CB-MAL22. The two clones were grown under various environmental gradients such as temperature (20, 30, 35 and 40$^{\circ}C$), salinity (0, 2, 5, 15 and 30psu), and $PO_4^{3-}$-P concentration (0, 1.6, 8.0, 40 and 200 ${\mu}M$M). Growth of CB-MAL21 and CB-MAL22 was measured by daily monitoring of chlorophyll fluorescence from each experimental culture for more than three serial transfers. Both the two experimental clones did not grow at 0psu. Maximal growth rates of the two clones were markedly reduced at lower $PO_4^{3-}$-P concentrations showing negligible growth at 0 and 1.6 ${\mu}M$M. However, growth of CB-MAL21 was not affected by low $NO_3^--$ concentration in culture media, showing the nitrogen-fixing ability. Maximum biomass yields of the two clones decreased dramatically at 35 and 40$^{\circ}C$. Optimal growth conditions for the two experimental clones were determined to be 20-30$^{\circ}C$, 40 ${\mu}M$M $PO_4^{3-}$-P, and wide salinity range from 5.0 to over 30psu. Best growth of CB-MAL21 was shown at (20$^{\circ}C$-15psu), which is less saline and cooler condition than those (i.e., 30$^{\circ}C$-30psu) for the best growth of CB-MAL22. The euryhaline and nitrogen-fixing CB-MAL21 strain thus can be a candidate laboratory culture for the future cyan bacterial marine biotechnology in temperate coastal waters.

• Kyungpook Mathematical Journal
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• 제45권1호
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• pp.33-43
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• 2005

Clones are sets of operations which are closed under composition and contain all projections. Identities of clones of term operations of a given algebra correspond to hyperidentities of this algebra, i.e., to identities which are satisfied after any replacements of fundamental operations by derived operations ([7]). If any identity of an algebra is satisfied as a hyperidentity, the algebra is called solid ([3]). Solid algebras correspond to free clones. These connections will be extended to so-called generalized clones, to strong hyperidentities and to strongly solid varieties. On the basis of a generalized superposition operation for terms we generalize the concept of a unitary Menger algebra of finite rank ([6]) to unitary Menger algebras with infinitely many nullary operations and prove that strong hyperidentities correspond to identities in free unitary Menger algebras with infinitely many nullary operations.

• Toxicological Research
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• 제22권4호
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• pp.323-328
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• 2006

Global gene expression profile was analyzed by microarray analysis of rat liver RNA after acute acetaminophen (APAP) administration. A single dose of 1g/kg body weight of APAP was given orally, and the liver samples were obtained after 24, 48 h, and 2 weeks. Histopathologic and biochemical studies enabled the classification of the APAP effect into injury (24 and 48 h) and regeneration (2 weeks) stages. The expression levels of 4900 clones on a custom rat gene microarray were analyzed and 484 clones were differentially expressed with more than a 1.625-fold difference(which equals 0.7 in log2 scale) at one or more time points. Two hundred ninety seven clones were classified as injury-specific clones, while 149 clones as regeneration-specific ones. Characteristic gene expression profiles could be associated with APAP-induced gene expression changes in lipid metabolism, stress response, and protein metabolism. We established a global gene expression profile utilizing microarray analysis in rat liver upon acute APAP administration with a full chronological profile that not only covers injury stage but also later point of regeneration stage.

• Animal cells and systems
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• 제1권4호
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• pp.629-635
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• 1997

Previously we reported the migration and rearrangement of a chloroplast gene cluster into mitochondria. The exact genomic locations of the clusters, modes of the gene rearrangement and mechanisms of the interorganellar migration of the clusters have yet to be understood. The detailed analysis needs to include a larger region of DNA surrounding each cluster. To study DNA rearrangement and migration in more detail a cosmid library was constructed using the total rice genomic DNA including nuclear, chloroplast and mitochondrial DNA. From this cosmid library, a sub-library was obtained by selecting the clones hybridized to various regions of chloroplast DNA. According to the hybridization pattern 136 clones from the sub-library were classified into 29 groups. Detailed analysis of these clones revealed that in addition to authentic chloroplast DNA, the clones contain its homologs resulted from rearrangement and mutation. We analyzed two clones in detail, which contain different rp12 homologs resulted from rearrangement and/or migration, respectively.

• Journal of Plant Biology
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• 제36권1호
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• pp.105-112
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• 1993

Selection of glyphosate-resistant clones from MNNG-treated mesophyll protoplasts of haploid tobacco and their differentiation were studied. The protoplasts were treated with 0.1 to 100 $\mu\textrm{g}$/mL N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for 30 min when they expanded to oval shapes. After the treatment, the protoplasts in 4-16 cell stages were transferred to the selective medium containing 1 mM glyphosate for the selection of the glyphosate-resistant colonies. The efficiency of the cell division of the protoplasts in the selective medium decreased as the MNNG concentrations in creased. Optimal MNNG concentration for induction of the glyphosate-resistant clones was 10$\mu\textrm{g}$/mL and mutation frequency was 2.66$\times$10-6. The stability of the glypohsate-resistance of the clones was examined by prolonged subculture in the medium with 1 mM glyphosate, and the resistant clones were survived more than 10 months. Among them one clone has been proliferating and greening and the others were proliferating without greening or greening with slower proliferating.

• 한국작물학회지
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• 제30권4호
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• pp.455-459
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• 1985

우수 이위체 clone을 선발하고 이를 FDR clone으로 육성하고자 본 실험 이 수행되었다. 1) 도입된 2X종자를 포장 및 온실에서 재배하여17 clone이 선발되었으며 이들의 특성이 조사되 고, 이들 Clone을 인공교배, 개방수분, 집단수분하여 그후대에서 18clone 이 선발되었다. 2) 선발된 clone들은 2n웅성과 2n자성배우자 형성에 대하여 조사되었다. 그중 D6-21만이 27∼30％의 2n웅성 배우자를 FDR에 의해 형성하였고. 다른 clone들은 2n웅성 배우자 형성율이 7％ 미만이었다. 3) 자성 2n배우자를 생성하는 clone은 발견되지 않았다.