• IMMUNE NETWORK
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• v.6 no.2
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• pp.67-75
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• 2006

Background: Cytotoxic function of killer cells is inhibited by specific recognition of class I MHC molecules on target cells by inhibitory killer Ig-like receptors (KIR) expressed on NK cells and some cytotoxic T cells. The inhibitory effect of KIR is accomplished by recruitment of SH2-containing protein tyrosine phosphatase (SHP) to the phosphotyrosine residues in the cytoplasmic tail. Methods: By in vitro coprecipitation experiments and transfection analysis, we investigated the association of KIR with an adaptor protein Shc in Jurkat T cells. Results: The cytoplasmic tail of KIR appeared to associate with an adaptor protein Shc in Jurkat T celilysates. Similar in vitro experiments showed that phosphorylated KIR cytoplasmic tail bound SHP-1 and Shc in Jurkat T cell lysates. The association of KIR with Shc was further confirmed by transfection analysis in 293T cells. Interestingly, however, Shc appeared to be replaced by SHP-2 upon engagement of KIR in 293T cells. Conclusion: Our data indicate that KIR associate with an adaptor protein Shc in Jurkat T cells, and suggest that KIR might have an additional role which is mediated by this adaptor protein.

• Journal of the Korean Institute of Educational Facilities
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• v.18 no.5
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• pp.43-50
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• 2011

There are emerging issues to update the educational environment for schools in terms of information and communication technology in order to provide customized programs to students as well as all participants relating to learning and teaching. The past year has been turbulent as the education facilities has changed and new procurement processes such as BTL have emerged. In this study, the feasibility analysis of ICT for the public educational environment is to analyse the current primary schools by means of collecting parent's opinion. In the web-site questionnaires, it was designed with 70 items such as teaching method, class organization, aptitude drill and educational community. As results, the statistical analysis is to propose the list of priority and orientation covering social agenda in the issue of ICT for education, the benefits schools can achieve by smart environment is to have the advanced learning services and solutions that represents parental engagement with identical local aims of interactive interface between their students and qualified teachers at a school. Both the national curriculum as well as the after-school program initiatives from the ministry of education, science and technology may reduce negative effects of private education so that the program has to be carefully developed for balanced education society revitalizing mutual communication within regional learning participants such as students, teachers and educational experts.

• The Korean Journal of Emergency Medical Services
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• v.23 no.2
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• pp.61-73
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• 2019

Purpose: This study aimed to introduce active learning methods, including flipped, case-based, and team-based learning in an electrocardiography (ECG) course and to investigate outcomes and satisfaction with these methods. Methods: To identify the learning effect of active learning, pre-and post-academic self-efficacy was compared between the experimental and control groups. In the experimental group, pre-and post-knowledge and clinical performance regarding ECG were also assessed. In addition, class satisfaction was investigated after application of active learning methods in the experimental group. Data were collected from 84 paramedic students and analyzed using SPSS 22.0 (IBM, Armonk, NY, USA). Results: The experimental group showed significant improvement in post-academic self-efficacy and knowledge. The experimental group also showed high clinical performance (9.83 out of 10 in ECG checking ability and 9.63 out of 10 in ECG reading ability). The mean satisfaction score was 4.23 out of 5 (responses based on a Likert scale) in the experimental group. Conclusion: Active learning in an ECG course was found to be highly effective and satisfactory. Furthermore, paramedic students can enhance their accountability and judgement with team-based learning through free engagement in discussion.

• Journal of Engineering Education Research
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• v.24 no.3
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• pp.21-31
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• 2021

Flipped learning, which involves listening to lectures at home and performing dynamic group-based problem-solving activities in the classroom, is recently evaluated as a learner-centered teaching method, and interest and applications in engineering education are increasing. Therefore, this study aims to provide practical guidelines for successful application through empirical research analysis on the use of flipped learning in domestic engineering education. Through the selection criteria and keyword search, a systematic review of 36 articles was conducted. As a result of the analysis, flipped learning research in engineering education has increased sharply since 2016, focusing on academic journals and reporting its application cases and effects. Most of the research supported that flipped learning was effective not only for learners' learning activities(e.g., academic achievement, satisfaction, engagement, learning-flow, interaction), but also for individualized learning and securing sufficient practice time. It was often used in major classes with 15 to less than 50 students, especially in computer-related major courses. Most of them consisted of watching lecture videos, active learning activities, and lectures by instructors, and showed differences in management strategies for each class type. Based on the analysis results, suggestions for effective flipped learning management in future engineering education were presented.

• Educational Technology International
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• v.23 no.1
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• pp.67-96
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• 2022

Although blogs have been used in online learning environments with optimistic expectations, the distributed nature of blogs can pose some challenges. Currently, we do not have a robust collection of tested blogging strategies to help students interact more effectively with each other when blogs are used as a primary form of engagement in an online class. Thus, the purpose of the study was to test an early iteration of an instructional blogging strategy, "Featured Student Profiles," which is designed to help students become acquainted with each other better and encourage them to visit and comment on each other's blogs. Sixteen pre-service teachers who were enrolled in an online course in which student blogs are the primary medium of peer interactions, participated in the study. Using a design case approach, seven students participated in interviews and all student blog interactions were analyzed. Thematic analysis was applied to analyze the interview data and identify salient themes of students' blogging experiences overall under the study strategy. The findings indicated that students took the most direct and efficient path they experienced to complete the blog task. Their peer interaction patterns varied, but several shifted from random to targeted relationships as the semester progressed. Although all students perceived the strategy as a positive approach to peer awareness, there was no clear evidence of its effect on student interactions.

• Journal of Practical Engineering Education
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• v.16 no.2
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• pp.143-150
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• 2024

With the development of AI, multimedia tools in education offer personalized learning environments, which foster individual competencies. This study aims to examine the effectiveness of smart education as perceived by learners through a case study of university writing classes utilizing multimedia learning tools, and to explore potential applications. To achieve this, a writing course incorporating various multimedia tools to promote interaction was designed and implemented over the course of one semester, targeting 42 university students. Through the semester, student reactions and survey results were analyzed to investigate the effects and satisfaction levels regarding the use of multimedia learning tools in writing instruction as perceived by students. The analysis revealed that multimedia-assisted writing classes effectively fostered learners' autonomy by focusing on individual needs, while also promoting interaction and encouraging spontaneous participation. Students reported recognizing the presence of diverse perspectives by comparing and communicating about each other's writing, leading to an expansion of their own thinking. In using ChatGPT, it was found that students attempted to refine their questions until they obtained the desired answers. They reported that this process deepened their understanding of the essence of the questions. These benefits led to results of high levels of students' active class engagement and satisfaction. This study contributes foundational and empirical data regarding the effectiveness and potential applications of learner-centered smart education as part of fourth industrial revolution integration research.

• Journal of Engineering Education Research
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• v.27 no.1
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• pp.41-49
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• 2024

The purpose of this study is to explore ways to improve major satisfaction that can be applied by universities through the analysis of factors influencing major satisfaction of engineering college students. To this end, Korea-National Survey of Student Engagement(K-NSSE) data involving 814 students from T University were used, and logistic regression analysis and t-test were applied. The main results obtained through this are as follows. First, engineering college students' major satisfaction factors include major-career relevance, college immersion, and positive academic sentiment. Second, depending on the grade, it was confirmed that the factor of major-career relevance in the lower grades, and the factors of meaningful learning experience and college immersion in addition to major-career relevance in the upper grades had a significant influence. Third, the higher the meaningful learning experience, positive academic sentiment, and college immersion, including the major-career relevance, the higher the major satisfaction was found in the middle-class group with a score of BO or higher. This study is meaningful in that it revealed differences in influence by individual characteristics as well as major satisfaction influencing factors that can be practiced in universities such as learning experiences.

• Korean Association of Arts Management
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• no.52
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• pp.5-37
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• 2019

Despite a copious volume of work on the relationship between social class and cultural consumption, scholars have paid scant attention to the increasingly apparent observation that a vast majority of the population exhibits indifference toward fine arts regardless of one's socio-economic status. Much of the prior literature on cultural consumption has treated the public's indifference to fine arts not as a distinct analytical category that deserves an explanation of its own, but simply as the opposite of "likes" or the act of consumption, let alone being disentangled from the concept of "dislikes" in taste-formation and consumption behavior. In this paper, we suggest that the seemingly increasing trend toward indifference to fine arts, especially among those who are part of the well-educated and economically well-off, merits close scholarly attention on its own term. As an initial step toward this endeavor, we explore the factors behind indifference toward fine arts among Korean middle-class, using the ground theory method. Our interview findings reveal that much of indifference toward fine arts is attributable to the lack of tastes in fine arts and artistic competence. Our results suggest that research drawing on Bourdieu's theory and Peterson's omnivore hypothesis needs to be further revised through an in-depth investigation of the institutional and societal contexts where art education takes place in Korea. We discuss the implications of our findings for policy-making in the cultural and artistic sphere.

• IMMUNE NETWORK
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• v.8 no.4
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• pp.130-136
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• 2008

Background: Human cytomegalovirus UL18, a MHC class I homologue, has been considered a natural killer (NK) cell decoy. It ligates LIR-1/ILT2 (CD85j), an NK inhibitory receptor, to prevent lysis of infected target cells. However, precise role of UL18 to NK cell cytotoxicity is yet elusive. Difficulty in clarifying the function of UL18 lies in complication in detecting UL18 mainly due to low level expression of UL18 on the surface and gradual loss of its expression. Methods: To overcome this hurdle, cDNA of cytoplasmic tail-less UL18 was constructed and expressed in swine endothelial cell (SEC). The expression level and its stability in the cell surface were monitored with FACS analysis. Results: Surface expression of UL18 is up-regulated by removing cytoplasmic tail portion from UL18F (a full sequence of UL18). SECs transfected with a cDNA of UL18CY (a cytoplasmic tail-less UL18) stably expressed UL18 molecule on the surface without gradual loss of its expression during 6 week continuous cultures. In the NK cytotoxicity assay, UL18 functions either inhibiting or activating NK cell cytotoxicity according to the source of NK cells. We found that there is individual susceptibility in determining whether the engagement of NK cell and UL18 results in overall inhibiting or activating NK cell cytotoxicity. Conclusion: In this study, we found that cytoplasmic tail is closely related to the regulatory function for controlling surface expression of UL18. Furthermore, by constructing stable cell line in which UL18 expression is up-regulated and stable, we provided a useful tool to clarify exact functions of UL18 on various immune cells having ILT2 receptor.

• Journal of the Korea Convergence Society
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• v.6 no.4
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• pp.15-23
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• 2015

The purpose of this study is to investigate how the impact on the basic psychological needs and emotional and motor sports commitment to participate in 12 weeks of Physically Disabled wheelchair tennis class. Results: First, the basic psychological needs is a difference between groups was found in the autonomy and the relationship of the sub-factors autonomy, competence, was the difference in time measured between the castle. Second, the movement was born immersed in the measuring point to see a difference in cognitive involvement and engagement of sub-behavior factors, the mutual effects were measured at the time of the two groups and sub-factors. Third, the movement was born in sentiment appears to have overlooked the measurement point in the sub-group differences in factors such as positive emotions and negative emotions, collective and cross effects were measured at the time of the two sub-factors. Subsequent studies in qualitative research, as well as be able to induce a more active ongoing participation of the disabled being difficult to draw parallel with the subjective experience of people with disabilities, it is thought to be possible to develop a variety of programs.