• The Korean Journal of Physiology and Pharmacology
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• 제18권5호
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• pp.403-409
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• 2014

The Bis protein is known to be involved in a variety of cellular processes including apoptosis, migration, autophagy as well as protein quality control. Bis expression is induced in response to a number of types of stress, such as heat shock or a proteasome inhibitor via the activation of heat shock factor (HSF)1. We report herein that Bis expression is increased at the transcriptional level in HK-2 kidney tubular cells and A172 glioma cells by exposure to oxidative stress such as $H_2O_2$ treatment and oxygen-glucose deprivation, respectively. The pretreatment of HK-2 cells with N-acetyl cysteine, suppressed Bis induction. Furthermore, HSF1 silencing attenuated Bis expression that was induced by $H_2O_2$, accompanied by increase in reactive oxygen species (ROS) accumulation. Using a series of deletion constructs of the bis gene promoter, two putative heat shock elements located in the proximal region of the bis gene promoter were found to be essential for the constitutive expression is as well as the inducible expression of Bis. Taken together, our results indicate that oxidative stress induces Bis expression at the transcriptional levels via activation of HSF1, which might confer an expansion of antioxidant capacity against pro-oxidant milieu. However, the possible role of the other cis-element in the induction of Bis remains to be determined.

• Molecules and Cells
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• 제26권5호
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• pp.496-502
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• 2008

Cryparin, encoded as a single copy gene (Crp) of the chestnut blight fungus Cryphonectria parasitica, is the most abundant protein produced by this fungus. However, its accumulation is decreased remarkably in C. parastica strains containing the double-stranded (ds) RNA virus Cryphonectria hypovirus 1. To characterize the transcriptional regulatory element(s) for strong expression and viral regulation, promoter analysis was conducted. Serial deletion of the Crp promoter region resulted in a step-wise decrease in promoter activity, indicating a localized distribution of genetic elements in the cryparin promoter. Promoter analysis indicated two positive and a repressive cis-acting elements. Among them, the promoter region between nt -1,282 and -907 appeared to be necessary for hypoviral-mediated down-regulation. An electrophoretic mobility shift assay (EMSA) on the corresponding promoter region (-1,282/-907) indicated two regions at (-1,257/-1,158) and (-1,107/-1,008) with the characteristic AGGAGGA-N42-GAGAGGA and its inverted repeat TCCTCTC-N54-TCCTCCT, respectively, appeared to be specific binding sites for cellular factors.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.77.2-78
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• 2003

An EREBP/AP2-type transcription factor (CaPFl) was isolated by DDRT-PCR following inoculation of soybean pustule pathogen Xanthomonas axonopodis pv. glycines Bra which induces HR on pepper leaves. Genomic Southern blot analysis revealed that the CaPFl gene is present as a single copy within the hot pepper genome. The deduced amino acid sequence of CaPFl has two potential nuclear localization signals, a possible acidic activation domain, and an EREBP/AP2 motif that could bind to a conserved cis- element present in promoter region of many stress-induced genes. The mRNA level of CaPFl was induced by both biotic and abiotic stresses. We observed higher-level transcripts in resistance-induced pepper tissues than diseased tissues. Expression of CaPFl is also induced upon various abiotic stresses including ethephon, MeJA, cold stress, drought stress and salt stress treatments. To study the role of CPFI in plant, transgenic Arabidopsis and tobacco plants which express higher level of pepper CaPFl were generated. Global gene expression analysis of transgenic Arabidopsis by cDNA microarray indicated that expression of CaPFl in transgenic plants affect the expression of quite a few GCC box and DRE/CRT box-containing genes. Furthermore, the transgenic Arabidopsis and tobacco plant, expressing CaPFl showed tolerance against freezing temperature and enhanced resistance to Pseudomonas syrnigae pv. tabaci. Taken together, these results indicated that CaPFl is a novel EREBP/AP2 transcription factor in hot pepper plant and it may has a significant role(s) in regulation of biotic and abiotic stresses in plant.

• BMB Reports
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• 제43권10호
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• pp.677-682
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• 2010

Kv4.2, a pore-forming $\alpha$-subunit of voltage-gated A-type potassium channels, is expressed abundantly in the soma and dendrites of hippocampal neurons, and is responsible for somatodendritic $I_A$ current. Recent studies have suggested that changes in the surface levels of Kv4.2 potassium channels might be relevant to synaptic plasticity. Although the function and expression of Kv4.2 protein have been extensively studied, the dendritic localization of Kv4.2 mRNA is not well described. In this study, Kv4.2 mRNAs were shown to be localized in the dendrites near postsynaptic regions. The dendritic transport of Kv4.2 mRNAs were mediated by microtubule-based movement. The 500 nucleotides of specific regions within the 3'-untranslated region of Kv4.2 mRNA were found to be necessary and sufficient for its dendritic localization. Collectively, these results suggest that the dendritic localization of Kv4.2 mRNAs might regulate the dendritic surface level of Kv4.2 channels and synaptic plasticity.

• 한국콘텐츠학회논문지
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• 제13권10호
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• pp.54-61
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• 2013

본 논문에서는 하드웨어 기반의 영상 신호 윤곽선 검출을 위한 하드웨어기반의 알고리즘으로 CMOS 이미지 센서의 인접픽셀과 아날로그 상관기로 구성되는 윤곽선 검출기를 제안한다. 제안하는 이미지 윤곽 검출기는 각 열(column)마다 비교기를 공유하고, 비교기는 기준전압과 비교를 통해 대상 픽셀의 윤곽선 여부를 판별한다. 이미지 센서와 직접적으로 연결된 윤곽선 검출 회로는 기존의 연구와 비교하여 면적은 4배, 그리고 전력소모는 20 % 감소하는 결과를 보였다. 또한 외부에서 기준전압을 제어할 수 있어, 윤곽선 검출의 민감도를 조절하기에 유용한 장점을 가진다. 0.18 ${\mu}m$ CMOS 공정에서 제작된 칩은 34%의 fill factor를 가지며, 픽셀 당 0.9 ${\mu}W$의 전력소모를 가진다.

• The Plant Pathology Journal
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• 제24권3호
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• pp.289-295
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• 2008

RNA-RNA interactions and the dynamic RNA conformations are important regulators in virus replication in several RNA virus systems and may also involved in the regulation of many important virus life cycle phases, including translation, replication, assembly, and switches in these important stages. The 5' non-translated region of Potato virus X(PVX) contains multiple cis-acting elements that facilitate various viral processes. It has previously been proposed that RNA-RNA interactions between various RNA elements present in PVX RNA genome are required for PVX RNA accumulation(Hu et al., 2007; Kim and Hemenway, 1999). This model was based on the potential base-pairing between conserved sequence elements at the upstream of subgenomic RNAs(sgRNAs) and at the 5' and 3' end of RNA genome. We now provide more evidence that RNA-RNA base-pairing between elements present at the 5' end and upstream of each sgRNA is required for efficient replication of genomic and subgenomic plus-strand RNA accumulation. Site-directed mutations introduced at the 5' end of plus-strand RNA replication defective mutant(${\Delta}12$) increasing base-pairing possibility with conserved sequence elements located upstream of each sgRNAs restored genomic and subgenomic plus-strand RNA accumulation and caused symptom development in inoculated Nicotiana benthamiana plants. Serial passage of a deletion mutant(${\Delta}8$) caused more severe symptoms and restored wild type sequences and thus retained possible RNA-RNA base-pairing. Altogether, these results indicate that the RNA element located at the 5' end of PVX genome involved in RNA-RNA interactions and play a key role in high-level accumulation of plus-strand RNA in vivo.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권2호
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• pp.133-137
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• 2003

Cells respond to an accumulation of unfolded proteins in the endoplasmic reticulum (ER) by increasing transcription of genes encoding molecular chaperones and folding enzymes. The information is transmitted from the ER lumen to the nucleus by intracellular signaling pathway, called the unfolded protein response (UPR). In Saccharomyces cerevisiae, such induction is mediated by the cis-acting unfolded response element (UPRE) which has been thought to be recognized by Hac1p transcription factor. We cloned the ATFC gene showing similarity with Hac1p, and then examined to determine whether ATFC gene product specifically binds to UPRE by electrophoretic mobility shift assays. ATFC gene product displayed appreciable binding ${to ^{32}}P-labelled$ UPRE. Therefore, we concluded that ATFC represents a major component of the putative transcription factor responsible for the UPR leading to the induction of ER-localized stress proteins.

• 대한가정학회지
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• 제42권8호
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• pp.95-112
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• 2004

Most of the Korean Diaspora who lived in Yunhaeju moved to Central Asia in 1937 following the deportation policy of Russia. The Korean Diaspora has maintained the traditional way of living for 140 years without a deep relationship with Korea. This study examined the dwellings of the Korean Diaspora of Kazakhstan in Central Asia through visiting their houses and conducting interviews. The results of the research were as follows. 1) The houses of the Korean Diaspora in Kazakhstan in early times consisted of Jungjigan which has Gudle and one bedroom. Gudle is the most traditional element of the Korean Diaspora's house and it is generally used as a place to gather family members. 2) The Korean Diaspora's houses in Kazakhstan were basically built according to Russian style but with a slightly different way of living inside. 3) The changing process of planning and building code of apartments in Kazakhstan is similar to that in Russia and other CIS nations. 4) Korean's food style is one mixed with Korean, Russian and Central Asian foods and Kimchi and Jang(bean paste) are the essential elements for most Koreans. 5) Koreans are very active to develop a relationship with Kazaks, Uighurs, and Russians and this enables the exchange of their culture eventually. This study is the first step to supply basic information for study of the Korean Diaspora in Central Asia and deeper research is necessary with a wide range in Russia.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.734-740
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• 2005

The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.

• 대한가정학회지
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• 제42권9호
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• pp.1-18
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• 2004

Most of the Korean diaspora who lived in Yunhaeju moved to Central Asia in 1937 following the policy of deportation of Russia. Uzbekistan is the representative place for Korean diaspora to have settled down in Central Asia at that time and mort Korean diaspora in Central Asia are living in Uzbekistan now. Korean diaspora have maintained a traditional way of living for 140 years without a deep relationship with Korea. This study examined the dwellings of the Korean diaspora of Uzbekistan in Central Asia by visiting their houses and conducting interviews. Results of the research were as following: 1) The houses of the Korean diaspora in Uzbekistan in early times consisted of 2-3 bedrooms with Gudle. Gudle is the most traditional element of the Korean diaspora's house and it has been used as a place for gathering family members. 2) Korean diaspora's houses in Uzbekistan were built according to Russian and Uzbek style but most of the Korean diaspora's have a mixed style with traditional Korean elements of living.3) The changing process of planning and building codes of apartments in Uzbekistan is similar to that in other CIS nations and all of Russia. 4) Korean's food style is a mixture of Korean, Russian and Central Asian foods. Kimchi and Jang (bean paste) are essential for most Koreans and most Koreans are making these at home. 5) Most Koreans have maintained a good relationship with the Uzbek people and Koreans are very active in gelling along well with all. This enables them to exchange their culture eventually in various parts. This study could be the first step to supply basic information for the study of the Korean diaspora in Uzbekistan. Deeper research over a wider range is needed in Russia.