• Title/Summary/Keyword: circular unit

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Function and Oligomerization Study of the Leucine Zipper-like Domain in P13 from Leucania separata Multiple Nuclear Polyhedrosis Virus

  • Du, Enqi;Yao, Lunguang;Xu, Hua;Lu, Songya;Qi, Yipeng
    • BMB Reports
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    • v.40 no.2
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    • pp.232-238
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    • 2007
  • The p13 gene is uniquely present in Group II nucleopolyhedroviruses (NPVs) and some granuloviruses, but not in Group I NPVs. p13 gene was first described by our laboratory in Leucania separatamultiple nuclear polyhedrosis virus (Ls-p13) in 1995. However, the functions of Ls-P13 and of its homologues are unknown. When Ls-p13 was inserted into Autographa californica nucleopolyhedrovirus, a Group I NPV, polyhedra yield was inhibited. However, this inhibition was prevented when the leucine zipper-like domain of Ls-p13 was mutated. To determine the cause of this marked difference between Ls-P13 and leucine zipper mutated Ls-P13 (Ls-P13mL), oligomerization and secondary structure analyses were performed. High performance liquid chromatography and yeast two-hybrid assays indicated that neither Ls-P13 nor Ls-P13mL could form oligomers. Informatics and circular dichroism spectropolarimetry results further indicated marked secondary structural differences between Ls-P13 and Ls-P13mL. The LZLD of Ls-P13 has two extended heptad repeat units which form a hydrophobic surface, but it is short of a third hydrophobic heptad repeat unit for oligomerization. However, the mutated LZLD of Ls-P13mL lacks the above hydrophobic surface, and its secondary structure is markedly different. This difference in its secondary structure may explain why Ls-P13mL is unable to inhibit polyhedra yield.

Speculation of Optical Cavity for Improving Optical Gas Sensor's Characteristics (광학적 가스센서 특성 향상을 위한 광 공동 구조의 고찰)

  • Yi, Seung-Hwan;Park, Jong-Seon
    • Journal of the Korean Institute of Gas
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    • v.12 no.4
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    • pp.63-68
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    • 2008
  • This paper describes about the simulation and the experimental results of optical cavity with curved mirror surface and vertical mirror surface to improve the light intensity and efficiency of the optical sensors. When we use the vertical mirror surface, the distribution of light reached to the filter surface of detector shows an elliptical shape. Whereas, the curved mirror surface focuses the light into circular shape. Therefore, due to focusing effects in case of using curved mirror surface, the light intensity per unit area has been improved. Consequently, the output voltage of gas sensor has been expected to increase. Based upon the simulation, the experiment of gas sensor has been conducted with $CO_2$ gas from 0ppm to 2,500 ppm at 250 ppm step and $25^{\circ}C$, 45%R.H. ambient. The output voltage of gas sensor that has a curved mirror surface increases approximately 200 mV than that of vertical mirror surface.

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Performance Evaluation on the Pipelines for an Automated Vacuum Waste Collection System (생활폐기물 자동집하시설 이송관망 성능평가)

  • Jang, Choon-Man;Lee, Sang-Moon
    • The KSFM Journal of Fluid Machinery
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    • v.18 no.5
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    • pp.26-32
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    • 2015
  • This paper describes performance evaluation of design parameters, air velocity inside a pipeline and pressure along a pipeline, using experimental measurements in an automated vacuum waste collection system. Automatic robot having six cameras is introduced to analyze the internal pipeline conditions whether waste accumulates at the bottom of the pipeline or not. Throughout the experimental measurements of the pipeline having the various shapes, it is found that pressure and internal air velocity linearly increase along the pipeline from a waste inlet to a waste collection station while air density decreases due to the air compression effect with high pressure. Although air velocity inside the pipeline at a waste inlet keeps design velocity range between 20 m/s and 30 m/s, it is noted that air velocity near the waste collection station exceeds maximum design velocity of 30 m/s. Pressure increase per unit length is changed from 17.6 Pa/m to 18.9 Pa/m, which depends on the air velocity inside the pipeline. From the investigation inside the pipeline with CCTV loaded on an automated robot, waste accumulated at the bottom of the pipeline is mainly found at the downstream of a circular curved pipe, an inclined pipe and a bended pipe.

STUDY ON THE CRITICAL MAINTENANCE PERIOD OF MILLIPORE FILTER MEMBRANE IN RATS (백서에서 Millipore filter membrane의 임계유지기간에 대한 연구)

  • Kim, Mi-Suk;Yeo, Hwan-Ho;Kim, Su-Gwan;Lim, Sung-Chul
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.28 no.4
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    • pp.274-279
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    • 2002
  • The purpose of this study is to evaluate the critical maintenance period of absorbable membrane for guided bone regeneration. Fortynine Sprague-Dawley rats weighing about 300g were divided into seven groups. An 8 mm circular full-thickness defect in calvarial bone was made and then cellular acetate porous filter (Millipore $filter^{(R)}$.) was placed on the calvarial bone defect. The filter was removed at 2, 3, 4, 5, 6, 8 and 11 weeks after placement. Rats were sacrificed at 12 weeks the placement of cellular acetate porous filter. The specimens were stained with Hematoxylin-Eosin and observed under light microscope. The amount of regenerated bone was measured from both margin of calvarial bone defect (unit : mm). The results were as follows. Bone regeneration of each experimental group was increased gradually and the bond defect was almost completely filled with new bone in 5-, 6-, 8-, and 11-week experimental group. Histologic findings showed mild inflammatory response and granulation tissue formation without apparent adverse effects on the healing process. In 11-week experimental group, the bone defect was completely filled with new bone containing abundant osteoid which was oriented to the dural side and contribute to bony thickening. We suggest that non-absorbable membrane and bioabsorbable membrane presumably should remain intact for longer than 5 weeks to be effective.

A PHOTOELASTIC STRESS ANALYSIS OF FIXED PARTIAL DENTURES WITH BICON IMPLANTS ON MANDIBULAR POSTERIOR AREA (하악구치부에서 Bicon 임플란트에 의해 지지되는 고정성 국소의치의 광탄성 응력분석)

  • Kang, Jong-Un;Kim, Nan-Young;Kim, Yu-Lee;Cho, Hye-Won
    • The Journal of Korean Academy of Prosthodontics
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    • v.42 no.4
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    • pp.412-424
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    • 2004
  • Statement of problem: Several prosthetic options are available for the restoration of multiple adjacent implants. A passively fitting prosthesis has been considered a prerequisite for the success and maintenance of osseointegration. Passivity is a particular concern with multiple implants because of documented inaccuracies in the casting and soldering process. One way to avoid this problem is to restore the implants individually, however, the restorations of individual adjacent impants requires careful adjustment of interproximal contacts. Purpose: The purpose of this study was to compare the stress distribution pattern and amount surrounding Bicon implants with individual crowns and splinted restorations. Material and method: A photoelastic model of a human partially edentulous left mandible with 3 Bicon implants($4{\times}11mm$) was fabricated. For non-splinted restorations, individual crowns were fabricated on 3 abutments ($4{\times}0.65mm,\;0^{\circ}$, 2.0 mm post, Bicon Inc., Boston, USA) After the units were cemented, 4 levels of interproximal contact tightness were evaluated: open, ideal ($8{\mu}m$ shim stock drags without tearing), medium($40{\mu}m)$), and heavy($80{\mu}m$). Splinted 3-unit fixed partial dentures were fabricated and cemented to the model. Changes in stress distribution under simulated non-loaded and loaded conditions(7.5, 15, 30 lb) were analyzed with a circular polaricope. Results: 1. Stresses were distributed around the entire body of fin in Bicon implants. 2. Splinted restorations were useful for distribution of stress around implants especially with higher loads. 3. By increasing the contact tightness between the individually restored three implants, the stress increased in the coronal portion of implants. Conclusions: Ideal adjustment of the contact tightness was important to reduce the stresses around individually restored Bicon implants.

Hatching Rate of Pacific Cod (Gadus macrocephalus) in a Large Volume Hatching Rate of Pacific Cod (Gadus macrocephalus) in a Large Volume of the Hatching Jar (대용량 부화기를 이용한 대구 Gadus macrocephalus 수정란부화율)

  • Gwak, Woo-Seok;Han, Dong-Hoon;Lee, So-Gwang
    • Korean Journal of Ichthyology
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    • v.22 no.3
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    • pp.195-200
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    • 2010
  • A large volume of hatching jar was tested whether it is an effective to hatch fertilized eggs of Pacific cod. The volume of hatching jar did not show any significant differences in survival of the fertilized eggs; 48.3% for the jar of 15 L and 50.4% for that of 42 L. Survival rate of the fertilized eggs in a large volume of hatching jar ranged from 40.0 to 71.2%, which was higher than those of conventional small circular tank. A higher survival could be achieved even though stocking density of fertilized eggs was as high as 5,000 mL/jar. Consequently, the tested hatching jar allowed for incubation of a greater number of eggs with higher survival rate in a much smaller space. In addition, it reduces costs and manpower, and requires a relatively small amount of water per individual unit (6,700 mL/min), and provides a way to incubate multiple rearing groups in a quarantine environment.

Thermal Resistant Characteristics of Flowable Backfill Materials Using Surplus Soil for Underground Power Utilities (굴착잔토를 재활용한 지중 전력케이블 유동성 뒤채움재의 열저항 특성)

  • Oh, Gidae;Kim, Daehong
    • Journal of the Korean GEO-environmental Society
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    • v.11 no.10
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    • pp.15-24
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    • 2010
  • In the case of underground power utilities pipe such as circular pipe, the most difficult problem is low compaction efficiency of the bottom of pipe inducing the failure of utilities. To overcome this problem, various studies have been performed and one of these is CLSM(Controlled Low Strength Materials) accelerated flow ability. But underground power utilities pipe backfill materials is also needed to have good thermal property that can dissipate the heat as rapidly as it is generated. So, in this study, we performed thermal resistancy test for various materials such as sand, weathered soil, clay and mixed soil to analyze the thermal characteristics of CLSM(Controlled Low Strength Materials) with accelerated flow ability for various conditions(water content, unit weight, void ratio, curing time) and to evaluate the applicability for backfill material of underground power utilities pipe. The test results of 16 specimens for thermal resistancy test showed good thermal property that maintained below $85^{\circ}C\;cm/W$.

Design of Flight Learning System Using Sketch-based Interface (스케치 인터페이스를 이용한 항공기동 학습 시스템 개발)

  • Kim, Sang-Jin;Park, Tae-Jin;Choy, Yoon-Chul
    • Journal of Korea Multimedia Society
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    • v.13 no.5
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    • pp.771-779
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    • 2010
  • Sketch-based interface is used more and more in developing animation contents. Particularly, there has been a system where the user's sketch inputs are interpreted and presented as live motions. In this study¸ it is to design an animated flight learning system using sketch-based interface. Most of the flights include movements in three-dimensional space and have unique and complex flight patterns. In other words, the actual flight movements not only include acceleration and deceleration, rising and falling, straight or circular flying, but also may include combinations of two or more movements as they simultaneously occur such as accelerating while falling, or slowing down while rising, and so forth. And, currently existing flight learning animation system cannot present such complex flight patterns to the pilots of aircrafts or to those personnel for air-traffic controllers. Hence, it is to be shown in this study that unit-path sketch animation method can support quicker ways to create animations to present those complex flight movements, and requires lesser inputs compared to the existing frame-based animation method. Also, the flight learning system suggested uses the flight-route realization tasks to reflect complex flight patterns, and therefore creates animations close to real as possible.

Fundamental Experiment for the Development of Water Pipeline Locator (상수도관로 위치탐사 장비개발을 위한 기초실험)

  • Park, Sang-Bong;Kim, Jin-Won;Oh, Kyeong-Seok;Kim, Min-Cheol;Koo, Ja-yong
    • Journal of Korean Society of Water and Wastewater
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    • v.30 no.3
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    • pp.253-261
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    • 2016
  • A variety of methods for detecting the location of an underground water pipeline are being used across the world; the current main methods used in South Korea, however, have the problems of low precision and efficiency and the limitations in actual application. On this, this study developed locator capable of detecting the location of a water pipe by the use of an IMU sensor, and technology for using the extended karman filter to correct error in location detection and to plot the location on the coordinate system. This study carried out a tract test and a road test as basic experiments to measure the performance of the developed technology and equipment. As a result of the straight line, circular and ellipse track tests, the 1750 IMU sensor showed the average error of 0.08-0.11%; and thus it was found that the developed locator can detect a location precisely. As a result of the 859.6-m road test, it was found that the error was 0.31 m in case the moving rate of the sensor was 0.3-0.6 m/s; and thus it was judged that the equipment developed by this study can be applied to long-distance water pipes of over 1 km sufficiently. It is planned to evaluate its field applicability in the future through an actual pipe network pilot test, and it is expected that locator capable of detecting the location of a water pipe more precisely will be developed through research for the enhancement of accuracy in the algorithm of location detection.

Characteristics of Particle Separation in Suspension using an Ultrasonic Standing Wave

  • Shin, Beom-Soo;Danao, Mary-Grace C.
    • Journal of Biosystems Engineering
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    • v.37 no.2
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    • pp.113-121
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    • 2012
  • Purpose: Particle separation in solution is one of important process in a unit operation as well as in an extract preparation for biosensors. Contrary to centrifuge-type of mesh-type filter, using an ultrasonic standing wave make the filtering process continuous and free from maintenance. It is needed to investigate the characteristics of particle movement in the ultrasonic standing wave field. Methods: Through the computer simulation the effects of major design and driving parameters on the alignment characteristics of particles were investigated, and a cylindrical chamber with up-stream flow type was devised using two circular-shape PZTs on both sides of the chamber, one for transmitting ultrasonic wave and the other for just reflecting it. Then, the system performance was experimentally investigated as well. Results: The speed of a particle to reach pressure-node plane increased as the acoustic pressure and size of particle increased. The maximum allowable up-stream flow rate could be calculated as well. As expected, exact numbers of pressure-node planes were well formed at specific locations according to the wavelength of ultrasonic wave. As the driving frequency of PZT got close to its resonance frequency, the bands of particles were observed clearer, which meant the particles were trapped into narrower space. Higher excitation voltages to the PZT produced a greater acoustic force with which to trap particles in the pressure-node planes, so that the particles gathered could move upwards without disturbing their alignments even at a higher inlet flow rate. Conclusions: This research showed the feasibility of particle separation in solution in the continuous way by an ultrasonic standing wave. Further study is needed to develop a device to collect or harvest those separated particles.