Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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STUDY ON THE CRITICAL MAINTENANCE PERIOD OF MILLIPORE FILTER MEMBRANE IN RATS

백서에서 Millipore filter membrane의 임계유지기간에 대한 연구

  • Kim, Mi-Suk (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Yeo, Hwan-Ho (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Kim, Su-Gwan (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Lim, Sung-Chul (Department of Pathology, Medical Research Institute, College of Medicine, Chosun University)
  • 김미숙 (조선대학교 치과대학 구강악안면외과학교실 조선대학교 치과대학 구강생물학연구소) ;
  • 여환호 (조선대학교 치과대학 구강악안면외과학교실 조선대학교 치과대학 구강생물학연구소) ;
  • 김수관 (조선대학교 치과대학 구강악안면외과학교실 조선대학교 치과대학 구강생물학연구소) ;
  • 임성철 (조선대학교 의과대학 병리학교실)
  • Published : 2002.08.31
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Abstract

The purpose of this study is to evaluate the critical maintenance period of absorbable membrane for guided bone regeneration. Fortynine Sprague-Dawley rats weighing about 300g were divided into seven groups. An 8 mm circular full-thickness defect in calvarial bone was made and then cellular acetate porous filter (Millipore $filter^{(R)}$.) was placed on the calvarial bone defect. The filter was removed at 2, 3, 4, 5, 6, 8 and 11 weeks after placement. Rats were sacrificed at 12 weeks the placement of cellular acetate porous filter. The specimens were stained with Hematoxylin-Eosin and observed under light microscope. The amount of regenerated bone was measured from both margin of calvarial bone defect (unit : mm). The results were as follows. Bone regeneration of each experimental group was increased gradually and the bond defect was almost completely filled with new bone in 5-, 6-, 8-, and 11-week experimental group. Histologic findings showed mild inflammatory response and granulation tissue formation without apparent adverse effects on the healing process. In 11-week experimental group, the bone defect was completely filled with new bone containing abundant osteoid which was oriented to the dural side and contribute to bony thickening. We suggest that non-absorbable membrane and bioabsorbable membrane presumably should remain intact for longer than 5 weeks to be effective.

Keywords

References

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