• The Journal of Engineering Geology
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• v.26 no.4
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• pp.439-445
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• 2016

The new airports apply the systematic runway foundation construction to build the high quality runways in order to take the leading position to win future aerial demands and stay ahead of competitors. This study is intended to supplement the weakness of existing standard compaction test to minimize residual settlement of lower weak foundation during operation of passenger berthage. The fill material was sampled from 4 construction sites using the fill material with diameter of 100mm or less, and the standard compacting test (KS F 2312), large circular mold compacting test, and water-replacement field density test (ASTM D 5030) were conducted. The regression analysis of correlation of the field density test and the standard indoor compaction test showed the unreliable value at P-value of 0.05, and the regression analysis of the field density test and the large indoor compaction test showed the high correlation with R value of 0.8878. It is judged that the construction of overall uniform quality can be assured as the site condition is truly reflected only if the compacting test method is selected in consideration of maximum size of fill material when evaluating the maximum dry density used in design and construction.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.09a
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• pp.133-144
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• 2009

Incheon Bridge, 18.4 km long sea-crossing bridge, will be opened to the traffic in October 2009 and this will be the new landmark of the gearing up north-east Asia as well as the largest & longest bridge of Korea. Incheon Bridge is the integrated set of several special featured bridges including a magnificent cable-stayed girder bridge which has a main span of 800 m width to cross the navigation channel in and out of the Port of Incheon. Incheon Bridge is making an epoch of long-span bridge designs thanks to the fully application of the AASHTO LRFD (load & resistance factor design) to both the superstructures and the substructures. A state-of-the-art of the geotechnologies which were applied to the Incheon Bridge construction project is introduced. The most Large-diameter drilled shafts were penetrated into the bedrock to support the colossal superstructures. The bearing capacity and deformational characteristics of the foundations were verified through the world's largest static pile load test. 8 full-scale pilot piles were tested in both offshore site and onshore area prior to the commencement of constructions. Compressible load beyond 30,000 tonf pressed a single 3 m diameter foundation pile by means of bi-directional loading method including the Osterberg cell techniques. Detailed site investigation to characterize the subsurface properties had been carried out. Geotextile tubes, tied sheet pile walls, and trestles were utilized to overcome the very large tidal difference between ebb and flow at the foreshore site. 44 circular-cell type dolphins surround the piers near the navigation channel to protect the bridge against the collision with aberrant vessels. Each dolphin structure consists of the flat sheet piled wall and infilled aggregates to absorb the collision impact. Geo-centrifugal tests were performed to evaluate the behavior of the dolphin in the seabed and to verify the numerical model for the design. Rip-rap embankments on the seabed are expected to prevent the scouring of the foundation. Prefabricated vertical drains, sand compaction piles, deep cement mixings, horizontal natural-fiber drains, and other subsidiary methods were used to improve the soft ground for the site of abutments, toll plazas, and access roads. Light-weight backfill using EPS blocks helps to reduce the earth pressure behind the abutment on the soft ground. Some kinds of reinforced earth like as MSE using geosynthetics were utilized for the ring wall of the abutment. Soil steel bridges made of corrugated steel plates and engineered backfills were constructed for the open-cut tunnel and the culvert. Diverse experiences of advanced designs and constructions from the Incheon Bridge project have been propagated by relevant engineers and it is strongly expected that significant achievements in geotechnical engineering through this project will contribute to the national development of the longspan bridge technologies remarkably.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.11
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• pp.501-509
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• 2019

The construction of foundation piles for buildings and bridges is changing from pile driving to an injected precast pile method. The goal is to minimize environmental damage, noise pollution, and complaints from neighboring residents. However, it is necessary to develop economic piles that are optimized for precasting by a centrifugal method in terms of both the material and structural system. A reinforced joint method is proposed for reinforced concrete piles (RC piles) manufactured by centrifugal casting. A previous study concluded that the structural performance of the current joint system for RC piles could be improved by using a reinforced joint composed of extended circular band plates and studs. In this study, the structural performance of such a joint was validated experimentally by bending and shear strength measurements. The proposed joint reinforcement method showed adequate structural performance in terms of bending and shear strength. The overall load-deflection behavior is close to that of a structure without joints, so it is expected that the behavior and performance of the design can be reliably reflected in site structures.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.14 no.5
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• pp.88-95
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• 2015

The thickness of adsorbed molecular layers is the most critical factor in studying thin-film lubrication, and it is the most essential parameter that distinguishes thin-film from thick-film lubrication analysis. The thin film between the shaft and bearing surface within a very narrow gap was considered. The general Reynolds equation has been derived for calculating thin-film lubrication parameters affecting the performance of the circular journal bearing. Investigation of the load-carrying capacity and pressure distribution for the journal bearing considering the adsorbed layer thickness has been carried out. A Reynolds equation appropriate for the journal bearing is used in this paper for the analysis, and it is discussed using the finite difference method of the central difference scheme. The parameters, such as eccentricity and attitude angle, are used for discussing the load-carrying capacity of the journal bearing. The results reported in this paper should be applied to analysis of the journal bearing with different lubrication factors. The steady-state analysis of the journal bearing is conducted using the Reynolds model under thin-film lubrication conditions. For a journal bearing, several parameters, such as a pressure, load capacity, and pressure components of the bearing can be obtained, and these results can be stored in a sequential data file for later analysis. Finally, their distribution can be displayed and analyzed easily by using the MATLAB GUI technique. The load-carrying capability of the journal bearing is observed for the specified operating conditions. This work could be helpful for the understanding and research of the mechanism of thin-film lubrication.

• Journal of the Korean Magnetic Resonance Society
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• v.19 no.3
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• pp.106-111
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• 2015

Helicobacter pylori (H. pylori) survives in acidic and fluctuating pH conditions of the stomach. The pH effect on H. pylori proteins is important for the advanced understanding of its evolution and viability, although this bacterium has the molecular machinery that neutralizes the acidic condition. HP1492 is known as a conserved NifU-like protein from H. pylori. NifU is a nitrogen fixation protein that mediates the transfer of iron-sulfur (Fe-S) cluster to iron-sulfur proteins like ferredoxin. Commonly, the monomeric reduced state of NifU can be converted to the dimeric oxidized state by intermolecular disulfide bond formation. Because it remains unclear that HP1492 actually behaves as known NifU protein, we first found that this protein can adopt both oxidized and reduced forms using size exclusion chromatography. Circular dichroism experiment showed that HP1492 is relatively well-structured at pH 6.5, compared to other pH conditions. On the basis of the backbone resonance assignment of HP1492, we further characterized the residues that are sensitive to pH using NMR spectroscopy. These residues showing large chemical shift changes could be mapped onto the secondary structure of the protein. Our results could provide the foundation for structural and biophysical studies on a wide spectrum of NifU proteins.

• Magazine of the Korean Society of Agricultural Engineers
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• v.30 no.1
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• pp.38-49
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• 1988

This paper presented as follows results of laboratory model tests with various shaped footings on soil bed reinforced with the strips on the base of behaviour of soil structure according to the loads and triaxial test results reinforced with geotextiles. Their parameters studied were the effects on the bearing capacity of a footing of the first layer of reinforcement, horizontal and vertical spacing of layers, number of layers, tensile strength of reinforcement and iclination load to the vertical 1.Depending on the strip arrangement, ultimate bearing capacity values could be more improved than urreinforced soil and the failure of soil was that the soil structure was transfered from the macrospace to microspase and its arrangement, from edge to edge to face to face. 2.The reinforcement was produced the reinforcing effects due to controlling the value of factor of one and permeable reinforcement was never a barrier of drainage condition. 3.Strength ratio was decreased as a linear shape according to increment of saturation degree of soil used even though at the lower strength ratio, the value of M-factor was rot influenced on the strength ratio but impermeable reinforcement decreased the strength of bearing capacity. 4.Ultimate bearing capacity under the plane-strain condition was appeared a little larger than triaxial or the other theoretical formulars and the circular footing more effective. 5.The maximum reinforcing effects were obtained at U I B=o.5, B / B=3 and N=3, when over that limit only acting as a anchor, and same strength of fabric appeared larger reinforcing effects compared to the thinner one. 6.As the LDR increased, more and more BCR occurred and there was appeared a block action below Z / B=O.5, but over the value, decrement of BCR was shown linear relation, and no effects above one. 7.The coefficient of the inclination was shown of minimum at the three layers of fabrics, but the value of H / B related to the ultimate load was decreased as increment of inclination degree, even though over the value of 4.5 there wasn't expected to the reinforcing effects As a consequence of the effects on load inclination, the degree of inclination of 15 per cent was decreased the bearing capacity of 70 per cent but irnproved the effects of 45 per cent through the insertion of geotextile.

• Structural Engineering and Mechanics
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• v.66 no.2
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• pp.273-283
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• 2018

Currently, the dynamic amplification effect of suction is described using the wind vibration coefficient (WVC) of external loads. In other words, it is proposed that the fluctuating characteristics of suction are equivalent to external loads. This is, however, not generally valid. Meanwhile, the effects of the ventilation rate of louver on suction and its WV are considered. To systematically analyze the effects of the ventilation rate of louver on the multi-dimensional WVC of ultra-large cooling towers under suctions, the 210 m ultra-large cooling tower under construction was studied. First, simultaneous rigid pressure measurement wind tunnel tests were executed to obtain the time history of fluctuating wind loads on the external surface and the internal surface of the cooling tower at different ventilation rates (0%, 15%, 30%, and 100%). Based on that, the average values and distributions of fluctuating wind pressures on external and internal surfaces were obtained and compared with each other; a tower/pillar/circular foundation integrated simulation model was developed using the finite element method and complete transient time domain dynamics of external loads and four different suctions of this cooling tower were calculated. Moreover, 1D, 2D, and 3D distributions of WVCs under external loads and suctions at different ventilation rates were obtained and compared with each other. The WVCs of the cooling tower corresponding to four typical response targets (i.e., radial displacement, meridional force, Von Mises stress, and circumferential bending moment) were discussed. Value determination and 2D evaluation of the WVCs of external loads and suctions of this large cooling tower at different ventilation rates were proposed. This study provides references to precise prediction and value determination of WVC of ultra-large cooling towers.

• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.8-15
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• 2021

More and more available fungal genome sequence data reveal a large amount of secondary metabolite (SM) biosynthetic 'dark matter' to be discovered. Heterogeneous expression is one of the most effective approaches to exploit these novel natural products, but it is limited by having to clone entire biosynthetic gene clusters (BGCs) without errors. So far, few effective technologies have been developed to manipulate the specific large DNA fragments in filamentous fungi. Here, we developed a fungal BGC-capturing system based on CRISPR/Cas9 cleavage in vitro. In our system, Cas9 protein was purified and CRISPR guide sequences in combination with in vivo yeast assembly were rationally designed. Using targeted cleavages of plasmid DNAs with linear (8.5 kb) or circular (8.5 kb and 28 kb) states, we were able to cleave the plasmids precisely, demonstrating the high efficiency of this system. Furthermore, we successfully captured the entire Nrc gene cluster from the genomic DNA of Neosartorya fischeri. Our results provide an easy and efficient approach to manipulate fungal genomic DNA based on the in vitro application of Cas9 endonuclease. Our methodology will lay a foundation for capturing entire groups of BGCs in filamentous fungi and accelerate fungal SMs mining.

• Journal of Microbiology and Biotechnology
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• v.30 no.12
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• pp.1905-1911
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• 2020

Homoserine dehydrogenase (HSD) catalyzes the reversible conversion of ʟ-aspartate-4-semialdehyde to ʟ-homoserine in the aspartate pathway for the biosynthesis of lysine, methionine, threonine, and isoleucine. HSD has attracted great attention for medical and industrial purposes due to its recognized application in the development of pesticides and is being utilized in the large scale production of ʟ-lysine. In this study, HSD from Bacillus subtilis (BsHSD) was overexpressed in Escherichia coli and purified to homogeneity for biochemical characterization. We examined the enzymatic activity of BsHSD for ʟ-homoserine oxidation and found that BsHSD exclusively prefers NADP+ to NAD+ and that its activity was maximal at pH 9.0 and in the presence of 0.4 M NaCl. By kinetic analysis, Km values for ʟ-homoserine and NADP+ were found to be 35.08 ± 2.91 mM and 0.39 ± 0.05 mM, respectively, and the Vmax values were 2.72 ± 0.06 μmol/min-1 mg-1 and 2.79 ± 0.11 μmol/min-1 mg-1, respectively. The apparent molecular mass determined with size-exclusion chromatography indicated that BsHSD forms a tetramer, in contrast to the previously reported dimeric HSDs from other organisms. This novel oligomeric assembly can be attributed to the additional C-terminal ACT domain of BsHSD. Thermal denaturation monitoring by circular dichroism spectroscopy was used to determine its melting temperature, which was 54.8℃. The molecular and biochemical features of BsHSD revealed in this study may lay the foundation for future studies on amino acid metabolism and its application for industrial and medical purposes.

• Smart Structures and Systems
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• v.29 no.2
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• pp.267-278
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• 2022

Pre-stressed concrete circular spun piles are widely used in various infrastructure projects around the world and offer an economical deep foundation system with consistent and superior quality compared to cast in-situ and other concrete piles. Conventional methods for measuring the lateral response of piles have been limited to conventional instrumentation, such as electrical based gauges and pressure transducers. The problem with existing technology is that the sensors are not able to assist in recording the lateral stiffness changes of the pile which varies along the length depending on the distribution of the flexural moments and appearance of tensile cracks. This paper describes a full-scale bending test of a 1-m diameter spun pile of 30 m long and instrumented using advanced fibre optic distributed sensor, known as Brillouin Optical Time Domain Analysis (BOTDA). Optical fibre sensors were embedded inside the concrete during the manufacturing stage and attached on the concrete surface in order to measure the pile's full-length flexural behaviour under the prescribed serviceability and ultimate limit state. The relationship between moments-deflections and bending moments-curvatures are examined with respect to the lateral forces. Tensile cracks were measured and compared with the peak strains observed from BOTDA data which corroborated very well. By analysing the moment-curvature response of the pile, the structure can be represented by two bending stiffness parameters, namely the pre-yield (EI) and post-yield (EI_cr), where the cracks reduce the stiffness property by 89%. The pile deflection profile can be attained from optical fibre data through closed-form solutions, which generally matched with the displacements recorded by Linear Voltage Displacement Transducers (LVDTs).