• Food Engineering Progress
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• v.22 no.4
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• pp.304-308
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• 2018

The interest in and development of healthy foods and nutraceuticals have increased because of the trend for a health-oriented society. Cinnamon is used as a food ingredient as well as a herbal medicine because of its functional properties. In this study, flavoring compounds and antioxidative activities of cinnamon extracts were investigated with different extraction solvents and extraction methods. The contents of flavoring compounds such as coumarin, cinnamic acid, cinnamaldehyde, and cinnamyl alcohol were investigated. The contents of courmarin, cinnamic acid, and cinnamylaldehyde in 70% ethanol extract were higher than those in hot water and subcritical water extracts. The contents of courmarin, cinnamic acid, and cinnamaldehyde in subcritical water extract were higher than those in hot water extract, whereas the content of cinnamyl alcohol was lower. DPPH scavenging activity increased with increasing concentration of the extracts, and the 70% ethanol extract showed the highest antioxidant activity. The ascorbic acid content of the 70% ethanol extract was largest in the antioxidative activity measurement by FRAP analysis. The ascorbic acid contents of the hot water and subcritical water extracts were similar.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.6
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• pp.348-354
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• 2020

Oryeong-san (ORS) is a traditional Korean herbal medicine widely used for renal associated diseases, composed of five medicine herbs; Atractylodes japonica Koidzumi, Cinnamomum cassia Presl, Polyporus umbellatus Fries, Poria cocos Wolf and Alisma orientale Juzepzuk. We studied to improve the convenience of intake and portability by developing modernized dosage forms, and examined the effect on anti-inflammation of ORS. In order to develop the tablet formulation of ORS (ORS-F), the tablets were evaluated on the basis of physical characteristics include diameter, thickness, weight variation, hardness, friability and disintegration. To analyze the marker components of ORS-F, eight index markers from five herbal medicines were chosen. And the method using high performance liquid chromatography (HPLC) with diode-array detector method was established for the simultaneous analysis. The biological activities were examined the effect of ORS-F on pro-inflammation mediated by LPS-stimulation. The production of nitric oxide (NO) and cytokines were determined by reacting cultured medium with griess reagent and enzyme-linked immunosorbent assay (ELISA). The expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) were investigated by Western blot and RT-PCR. The anti-oxidant activities of OJS-F increased markedly, in a dose-dependent manner. and, The total phenolic compound and flavonoids contents of OJS-F were 10.20±0.09 ㎍/㎎ and 12.86±0.86 ㎍/㎎. OJS-F which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Therefore, the developed formulation for tablet of ORS-F provide efficiency and usability, and indicated effect of anti-inflammation.

• The Korea Journal of Herbology
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• v.34 no.6
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• pp.79-89
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• 2019

Objective : This study investigated the anti-diabetic effects of DM1, a herbal mixture with Atractylodis Rhizoma, Anemarrhenae Rhizoma, and Cinnamomi Cortex in high fat diet (HFD)-induced diabetic mice and the mechanism in C2C12 mouse skeletal muscle cells. Methods : The C57B/6 mice were fed high fat for 12 weeks, and then administrated DM1 extract (500 mg/kg, p.o.) for 4 weeks. The changes of body weight, calorie and water intakes, fasting blood glucose levels and the serum levels of glucose, insulin, triglyceride, HDL-cholesterol, AST and ALT were measured in mice. The histological changes of liver and pancreas tissues were also observed by H&E stain. C2C12 myoblasts were differentiated into myotubes and then treated with DM1 extract (0.5, 1, and 2 mg/㎖) for 24 hr. The expression of myosin heavy chain (MHC), PGC1α, Sirt1 and NRF1, and the AMPK phosphorylation were determined in the myotubes by western blot, respectively. Results : The DM1 extract administration significantly decreased the calorie and water intakes, glucose, triglyceride, AST and ALT levels and increased insulin and HDL-cholesterol in HFD-induced diabetic mice. DM1 extract inhibited lipid accumulation in liver tissue and improved glucose tolerance. In C2C12 myotubes, DM1 treatment increased the expression of MHC, PGC1α, Sirt-1, NRF-1 and the AMPK phosphorylation. Conclusion : In our results indicate that DM1 can improve diabetic symptoms by decreasing the obesity, glucose tolerance and fatty liver in HFD-induced diabetic mice, and responsible mechanism is might be related with energy enhancement.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.87-101
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• 2022

This study analyzed changes in the vegetation structure of 16 permanent plots (experimental and control) installed in Wando Arboretum in 1996 to monitor the long-term change process of evergreen broad-leaved forests in warm temperate. Especially, this study discusses the effects of trial treatment (selection cutting and plant introduction), introduced as a restoration technique in 1996, on vegetation development. In communities dominated by Quercus acuta in the canopy (permanent plots 1 through 3), this species's mean important percentage (MIP) decreased, and the evergreen broad-leaved species was introduced from outside increased, likely to change the vegetation structure in the future. The expansion of the evergreen broad-leaved species group was also confirmed in Q. acuta-deciduous broad-leaved forests (permanent plots 5 and 7) and Pinus densifloraforests (permanent plots 9 and 10). In the experimental plots where thinning was carried out, the zoochory (the dispersal of seeds by birds), Cinnamomum yabunikkei, Neolitsea sericea, Machilus thunbergii, etc., and the expansion of the influence of evergreen broad-leaved species were remarkable, so it is considered to have effectively promoted the vegetation development in warm temperate forests. Although evergreen broad-leaved species were planted in the experimental plot to change vegetation structure, it seems the effect on the change was weak due to the small amount of planting. Compared to other vegetation types, the change in the vegetative structure of the pine forest to an evergreen broad-leaved forest was clear due to the decline of P. densiflora and P. thunbergii.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.72-86
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• 2022

This study was conducted to identify structural characteristics of the evergreen broad-leaved forests distributed in Hak-dong, Geojae island. For a survey, 52 sites were set up in areas with changes in the vegetation community or location environment where Cinnamomum yabunikkei, Neolitsea sericea, and Machilus thunbergii dominated or appeared in the canopy, sub-canopy, or shrub layer. The community classification with TWINSPAN identified the following communities: N. sericea-C. yabunikkei, C. yabunikkei-Camellia japonica, Ca. japonica, Quercus variabilis-Ca. japonica, Pinus thunbergii-Ca. japonica, Castanopsis sieboldii, P. thunbergii, and Platycarya strobilacea-Mallotus japonicus. Considering the result of the study that succession series of warm-temperate forest reflecting the latent natural vegetation is the transition of conifers and deciduous broad-leaved forest to evergreen broad-leaved forest, the communities predominated by the communities predominated by the communities predominated by P. thunbergii, Q. variabilis, and Pl. strobilacea are likely to transform into the evergreen forest predominated by N. sericea and C. yabunikkei. The sites where C. yabunikkei, N. sericea, and Castanopsis sieboldii are dominant in the canopy and sub-canopy layers are likely to maintain the status quo if there is no artificial disturbance. The relationship between the impact of the environmental factors and the vegetation distribution showed silt among the physical properties of the soil directly or indirectly affected it, which was judged to be due to the fact that it was located on a steep slope. The soil acidity (pH) was 5-5.84, electrical conductivity 0.047-0.139 dS/m, and organic matter content was 3.32-12.06%. Although there were differences by the colony, they were generally low.

• Korean Journal of Environment and Ecology
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• v.27 no.2
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• pp.241-252
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• 2013

This study investigated plant community structure of Dongbaekdongsan(Mt.) in Jeju-do to identify character as evergreen broad-leaved forest. 20 plots(size is $20m{\times}20m$) were set up and plant communities were divided into 5 communities of Quercus glauca-Castanopsis cuspidata var. sievoldii-Ilex integra(Camellia japonica), Quercus glauca-Castanopsis cuspidata var. sievoldii-Camellia japonica, Quercus glauca-Camellia japonica, Quercus glauca-Camellia japonica-Pinus thunbergii and Pinus thunbergii-Quercus glauca by using TWINSPAN, DCA ordination, mean importance value and environmental condition. Area of Evergreen broad-leaved forest with stonny ground is covered with Quercus glauca and Castanopsis cuspidata var. sievoldii in canopy layer and Camellia japonica in the subordinate layer. Area of remaining soil's depth is covered with Pinus thunbergii. It has been alleged that Pinus thunbergii planted by human when Dongbaekdongsan(Mt.) is not selected local monuments. All of communities is predicted success to community of Quercus glauca-Camellia japonica. Shannon's diversity ranged from 1.0268 to 1.0717 in area of Evergreen broad-leaved forest and 1.2102 in area of Pinus thunbergii. 10 species of broad-leaved tree's constancy ratio is over the 80%. In future, Castanopsis cuspidata var. sievoldii and Cinnamomum japonicum can cover canopy layer, but it has low importance value. Specially high aged Camellia japonica is appeared everywhere. It has been alleged that it was touched by divinity since past. Dongbaekdongsan(Mt.) has different plant structure with another evergreen broad-leaved forest. It is hard that knowing its successional sere because it was damaged when it isn't degignate local monument. Accordingly continuous monitoring was required, successional direction and community's character is identified after forest become steady.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.545-549
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• 2001

To develop a natural antibacterial agent for fish bacterial diseases, antibacterial activity, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and bactericidal effect of cinnamon bark extract were examined against fish pathogenic bacteria. The antimicrobial effect of the extract to the fish diet was also estimated, Cinnamon bark extract showed the broad spectrum of antibacterial activity against fish pathogenic bacteria, especially, it had strong activity against Streptococcus iniae, Edwardsiella tarda and Listonella anguillarum. Its MIC was $75.8\sim189.6{\mu}g/mL$ against Cram positive bacteria, and $75.8\sim113.8{\mu}g/mL$ against Gram negative bacteria in liquid medium, It was found to show stronger bactericidal action against Gram negative bacteria than Cram positive bacteria. According to increasing concentrations of the extract, it resulted in a proportional reduction of viable cell counts of both S. iniae and L. anguillarum. The former was not detected by addition of $189.6{\mu}g/mL$ after 12 hours incubation and the latter by addition of $151.6{\mu}g/mL$ after 24 hours incubation, respectively. It was reasonable that fish diet was soaked in cinnamon bark extract for ten minutes. The relationship formula between the weight of fish diet and the extract absorbed to fish diet was Y=7.2726X+4.5083 ($R^2=0.9998$). The fish diet soaked in the extract inhibited the growth of all strains used in this study. Its antibacterial activity was stable at the range from $10^{\circ}C\;to\;35^{\circ}C$ during the storage period of 28 days. When the diet soaked in the extract was incubated in liquid medium at $35^{\circ}C$, it inhibited the growth of microorganisms inhabited in the diet.

• Journal of Sasang Constitutional Medicine
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• v.11 no.1
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• pp.201-220
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• 1999

We got these results after studying about modification of Gejitang(桂枝湯) that showed on sympton and prescription about Soumin(少陰人). 1. There are totally eleven prescriptions related to Gejitang appeared on Dongyi-Soose-Bowon(東醫壽世保元). Among them, one is prescription of Danguisayoktang(當歸四逆湯) in prescription of Discussion of Cold Induced Disorder(傷寒論) and another is Gejibujatang(桂枝附子湯) in Won-Myung dynasty period(元明時代). There are nine prescriptions in LeeJae-Ma(李濟馬)'s pre-scription and we can see there are many prescriptions used prescriptions related to Gejitang for Mang Yang(亡陽). 2. Pre-scriptions related to Gejitang has seven prescriptions in Extra-disease(表病證), and two prescriptions in Intra-disease(裏病證) which are analyzed as modified prescription from Gejitang. 3. Although 藿香正氣散, 香蘇散, 芎歸香蘇散, 八物君子湯 etc. are not modified prescriptions of Gejitang, We can see that its are brought up to be on the same with alternative remedy of Gejitang in each step of symptoms. 4. Symptoms are exactly classified in Soumin, the prescription which is used in different symptoms of diseases is similar in raw materials. It's because the remedy of Acending and Descending(升降) is used under the same purpose that maintain Yangnan Qi(陽暖之氣). 5. We could realize that Panax gienseng(人蔘), Cinnamomum Cassia(肉桂) and Aconitum Carrnichali(附子) are commonly important to reinforce Yangnan Qi because the more disease got worse, the more patient lost Yangnan Qi on both Extra-disease and Intra-disease in the Soumin's disease.

• Journal of Food Hygiene and Safety
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• v.13 no.2
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• pp.77-82
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• 1998

As a fundermental research for quality stailization of herb extract, the effects of water activity on microbial growth in herb extract were investigated. Herbs-Panax ginseng, Cinnamomum cassia, Lycium chinense, Zyzyphus jujuba, Lindera obtusilobum-were mixed and extracted with water at $80^{\circ}C$ and concentrated at $75^{\circ}C$. Water activity of the herb extract was adjusted to 0.86, 0.80 and 0.69, using water activity analyzer. The extracts were incubated for 180 days at $40^{\circ}C$ and then examined microbial cell counts and some physicochemical properties. In the extract of $a_{w}$ 0.86, 18 CFU/g of initial viable cell was increased to 80 CFU/g with 90 days of incubation and to 190 CFU/g 180 days of incubation. In the extract of $a_{w}$ 0.80, 24 CFU/g of initial viable cell was also increased to 83 CFU/g during the 90 days of incubation and to 170 CFU/ g for the 180 days of incubation. However, in the extract of $a_{w}$ 0.69, viable cell after 180 days of incubation was remained at almost the same level as initial viable cell. pH of herb extract was reduced in proportion to the decrease in water activity. The TLC (thin layer chromatography) patterns of ginseng saponins of herb extract did not show any significant changes after 180 days of incubation. Growth of pathogenic microorganisms was inhibited more with lower water activity of the herb extracts. In the herb extract inoculated with Candida albicans and Aspergillus niger, initial viable cells of 150 and 140 CFU/g were decreased to 30 and 20 CFU/g, repectively, after 30 days of incubation at $28^{\circ}C$. In the case of herb extract inoculated with Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, growth of the bacteria was totally inhibited even after 30 days of incubation at $37^{\circ}C$.

• Korean Journal of Plant Resources
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• v.26 no.5
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• pp.589-596
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• 2013

Vaccinium bracteatum Thunb. is an evergreen plant that belongs to the Genus Vaccinium distributed in Korea, and its fruit has been used for food by the common people and thus has a high possibility of commercialization. Therefore, the habitat environment and individual characteristics of V. bracteatum in Jeju Island were investigated to obtain the basic data for commercialization and preservation. The results showed that V. bracteatum is mostly distributed on the southern slope of Mt. Halla at an altitude of 100 to 500 m. V. bracteatum that occurred in the quadrat were total 30 individuals, which had an average height of 6.0 m, an average DBH of 7.8 cm, and an average number of trunk of 4.3. As for the community structure of the habitat, the average percentage of vegetation cover for the tree layer was 36%, while that for the subtree layer was 78%, which emphasized the importance of the subtree layer. The total number of species that occurred in the quadrat was 76. For the tree layer, total 10 species occurred, which include 2 species of evergreen coniferous trees, 3 species of evergreen broad-leaved trees, and 5 species of deciduous broad-leaved trees. For the subtree layer, total 24 species occurred, which include 1 species of evergreen coniferous tree, 11 species of evergreen broad-leaved trees, and 10 species of deciduous broad-leaved trees. Among these, Eurya japonica Thunb. was the species with a high relative contribution, which threatened V. bracteatum For the shrub layer, 27 species occurred including Cinnamomum japonicum Siebold, and for the herbaceous layer, 64 species occurred including Ardisia crenata Sims. The similarity between the habitats of V. bracteatum was very low, and it did not prefer a characteristic community. The V. bracteatum community around Shinrei stream was distributed on the surrounding area adjacent to the slope of the stream rather than the slope. There were a lot of basins, but the branches, which did not receive sunlight, had withered. It is thought that V. bracteatum would decline if the forest formation continues.