• 생명과학회지
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• 제28권5호
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• pp.587-596
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• 2018

전 세계적으로 담배흡연자의 심혈관 위험 감소와 금연을 위한 대안으로 전자담배 이용이 급속하게 확산되고 있지만 니코틴을 포함한 잠재적 유해화합물을 함유한 전자담배의 심혈관 효과는 불분명하다. 본 연구는 전자담배 사용에 따른 심장자율신경조절 변화를 관찰하고 급성적인 교감신경흥분효과 및 부교감신경활동 저하 반응을 궐련담배 흡연과 직접 비교하려는 목적으로 수행되었다. 41명의 젊고 건강한 흡연남성들을 대상으로 자료 분석을 수행하였으며, 무작위 교차 설계에 의해 1주의 wash-out period를 적용하여 전자담배와 궐련담배 세션이 각각 실시되었다. 전자담배는 흡연 시를 포함한 흡연 후 5분까지, 궐련담배의 경우 흡연 시 및 흡연 후 최대 30분까지 교감신경활성화 및 부교감신경 감소가 관찰되었다. 두 제품 간 비교에서, 흡연 시를 포함한 흡연 후 30분 동안의 HRV 분석에서 전자담배는 궐련담배에 비해 비교적 교감신경흥분효과 및 부교감신경저하에서 적은 변화수준 및 단시간 변화를 제공하는 것으로 나타났다. 이상의 결과들은 비록 전자담배가 궐련담배에 비해 심장자율신경조절에서 상대적으로 완화된 효과를 제공한다고 할지라도 전자담배 내 니코틴 및 잠재적 위험요인들을 고려해 볼 때 급성적인 교감신경흥분작용 및 부교감신경의 저하를 야기함으로 과도한 사용에 주의가 요구된다.

• Tuberculosis and Respiratory Diseases
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• 제78권1호
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• pp.8-17
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• 2015

Background: AMP-activated protein kinase (AMPK) not only functions as an intracellular energy sensor and regulator, but is also a general sensor of oxidative stress. Furthermore, there is recent evidence that it participates in limiting acute inflammatory reactions, apoptosis and cellular senescence. Thus, it may oppose the development of chronic obstructive pulmonary disease. Methods: To investigate the role of AMPK in cigarette smoke-induced lung inflammation and emphysema we first compared cigarette smoking and polyinosinic-polycytidylic acid [poly(I:C)]-induced lung inflammation and emphysema in $AMPK{\alpha}1$-deficient ($AMPK{\alpha}1$-HT) mice and wild-type mice of the same genetic background. We then investigated the role of AMPK in the induction of interleukin-8 (IL-8) by cigarette smoke extract (CSE) in A549 cells. Results: Cigarette smoking and poly(I:C)-induced lung inflammation and emphysema were elevated in $AMPK{\alpha}1$-HT compared to wild-type mice. CSE increased AMPK activation in a CSE concentration- and time-dependent manner. 5-Aminoimidazole-4-carboxamide-1-${\beta}$-4-ribofuranoside (AICAR), an AMPK activator, decreased CSE-induced IL-8 production while Compound C, an AMPK inhibitor, increased it, as did pretreatment with an $AMPK{\alpha}1$-specific small interfering RNA. Conclusion: $AMPK{\alpha}1$-deficient mice have increased susceptibility to lung inflammation and emphysema when exposed to cigarette smoke, and AMPK appears to reduce lung inflammation and emphysema by lowering IL-8 production.

• 한국연초학회지
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• 제10권1호
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• pp.49-57
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• 1988

The phenolic compounds were isolated from the cigarette smoke condensate using two immiscible organic solvents by a partition coefficient. Of them, 20 phenolic compounds were identified by GC and GC/MS. The amount of the phenolic compounds was phenol, p-crestol, hydroxybensoic acid in order. And there was no difference in composition of these compound dependent on leaf tobacco . About 70% amount of these compounds were absorbed to cellulose acetate filter used. The contents and composition of these compounds were various in the commercial cigarette.

• 한국연초학회지
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• 제23권1호
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• pp.77-81
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• 2001

This study was conducted to determine menthol migration from the tobacco to the filter and the change in percentage of menthol in pack for different mentholated cigarettes. Methanol was used to extract menthol from tobacco, filter and foil. A known weight of internal standard was added to each extract and then the extracts were analyzed by gas chromatography. After 4 days, 95% of the menthol applied to the foil was delivered into the tobacco and filter, but in case of menthol applied to the tobacco, the percentage of menthol that migrated from the tobacco to the filter was below 10%. After 100 days, 30-45% of the menthol in the tobacco had migrated into the filter. The amount of menthol in the pack decreased after 20 days of storage, however, the remaining menthol in cigarette treated with 0.30% propylene glycol was higher in amount than that with 0.12% propylene glycol In analysis on menthol transfer to mainstream smoke, the most efficient sample was the cigarette with menthol applied to the foil rather than to the tobacco.

• 접착 및 계면
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• 제11권4호
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• pp.137-143
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• 2010

Glass beads (GBs) were modified via plasma polymerization coatings in order to enhance the adsorption of polycyclic aromatic hydrocarbons (PAHs) in cigarette smoke and activated carbons (ACs) were also utilized for comparative purposes. First, GBs and ACs were subjected to surface modification via plasma polymerization coating of acrylic acid, acrylonitrile, 1,3-diaminopropane, thiophene or dimethylphosphite with a RF plasma (13.56 MHz) generator. Next, their adsorption behavior was evaluated with a home-made 4-port smoking machine by collecting the total particulate matters (TPMs) on a Cambridge filter pad, followed by the separation of PAHs via solid phase extraction and analysis with GC/MS. Finally, the plasma polymerization coatings were analyzed by FT-IR/ATR to elucidate the adsorption mechanism, while the topology of the modified GBs and ACs were studied by FE-SEM.

• 한국연초학회지
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• 제10권1호
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• pp.75-82
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• 1988

Cigarettes were made using a triple filter with several porous materials in its cavity. The removal effect of the adsorbents on carbon monoxide and hydrogen cyanide in cigarette smoke was investigated with the variation of their surface area and pore volume distributions. Several attempts were made to activated coconut shell based char under the fixed steam purging rate. 1. The specific surface area increased in number of micropore. It was found for transitional pore to have a little effect on the total surface area. 2. A Small amount of the particulate matter adsorbed on the adsorbents with transitional pores, Zeolite showed a little effect on the carbon monoxide adsorption though its small pore volume, but there was no significant difference in the adsorption capacity zeolite and the others. 3. In the adsorption for hydrogen cyanide as a vapor phase in cigarette smoke, the adsorption effect of the adsorbents increased remarkably with increasing their surface area and number of micropore. It was considered that the adsorbents with small pore volume like molecular seive 4A, in which the capillary diffusion of adsorbates could not be able, would not be effective for the adsorption of hydrogen cyanide.

• 한국연초학회지
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• 제28권2호
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• pp.144-151
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• 2006

A measurement uncertainty for analysis of volatile organic compound (benzene) in cigarette mainstream smoke was carried out. In this study one point re-calibration method was used to estimate uncertainty for benzene. The measurement uncertainty was calculated based on the uncertainty sources of each analysis step, quality appraisal sources, drift and repeatability. As a result, the concentration and expanded uncertainty of benzene in cigarette mainstream smoke were measured as $38.08{\pm}4.36{\mu}g/cig$. Relative uncertainty of drift and repeatability obtained were 5% and 3%, respectively.

• Journal of Preventive Medicine and Public Health
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• 제47권3호
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• pp.129-135
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• 2014

Tobacco use is the most important preventable risk factor for premature death. The World Health Organization (WHO) Framework Convention on Tobacco Control (FCTC), the first international public health treaty, came into force in 2005. This paper reviews the present status of tobacco control policies in Korea according to the WHO FCTC recommendations. In Korea, cigarette use is high among adult males (48.2% in 2010), and cigarette prices are the lowest among the Organization for Economic Cooperation and Development countries with no tax increases since 2004. Smoke-free policies have shown incremental progress since 1995, but smoking is still permitted in many indoor public places. More than 30% of non-smoking adults and adolescents are exposed to second-hand smoke. Public education on the harmful effects of tobacco is currently insufficient and the current policies have not been adequately evaluated. There is no comprehensive ban on tobacco advertising, promotion, or sponsorship in Korea. Cigarette packages have text health warnings on only 30% of the main packaging area, and misleading terms such as "mild" and "light" are permitted. There are nationwide smoking cessation clinics and a Quitline service, but cessation services are not covered by public insurance schemes and there are no national treatment guidelines. The sale of tobacco to minors is prohibited by law, but is poorly enforced. The socioeconomic inequality of smoking prevalence has widened, although the government considers inequality reduction to be a national goal. The tobacco control policies in Korea have faltered recently and priority should be given to the development of comprehensive tobacco control policies.

• 한국연초학회지
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• 제32권1호
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• pp.35-40
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• 2010

This study was carried out to enhance the analytical methods of phenolic compounds in mainstream cigarette smoke using high efficiency column and RRLC(Rapid Resolution Liquid Chromatography) system, and to compare these methods. RRLC system offers significantly faster results with higher data quality of phenolic compounds than conventional HPLC, but it is disadvantage that it is expensive. On the other hand, the method using monolithic column offers faster results by the use of conventional HPLC system without new equipment introduction. In this study, we used the linear type smoking machine and Health Canada method for pre-treatment process of phenolic compounds. The analysis time of phenolic compounds using RRLC and monolithic column was individually 8 and 15 minutes, whereas in the conventional HPLC it was 45 minutes. These new methods were accompanied with the minimal solvent consumption and had lower analysis costs. Also, we proved that there were no difference between new methods and conventional method in accuracy by statistic.

• 약학회지
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• 제55권3호
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• pp.251-259
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• 2011

Cigarette smoke condensate (CSC) is known to be carcinogenic compound. CSC contains many organic compounds such as polycyclic aromatic hydrocarbons (PAHs), and heterocyclic amine compounds (HCAs). Reactive oxygen species (ROS) are also generated and induce oxidative DNA damage during the metabolism of CSC. The rat microsome mediated and DNA repair enzyme treated comet assays together with conventional comet assay were performed to evaluate the mechanisms of CSC genotoxicity. The organic extract of CSC induced oxidative and microsome mediated DNA damage. Vitamin C as a model antioxidant reduced DNA damage in endonuclease III treated comet assay. One of flavonoid, galangin as a CYP1A1 inhibitor, reduced DNA damage in the presence of S-9 mixture. The ethanol extracts of the mixed vegetables (BV) or the mixed fruits (BF) showed potent inhibitory effects against CSC induced DNA damage with oxidative DNA lesions and in the prescence of S-9 mixture. These results indicate that BV and BF could prevent CSC-induced cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 in mammalian cells.