• 제목/요약/키워드: chung tea

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Role of Calcium and Protein Kinase C in Platelet Activating Factor-induced Activation of Peritoneal Macrophages (Platelet Activating Factor에 의한 대식세포의 활성화에 있어서 칼슘과 Protein Kinase C의 역할)

  • Lee, Chung-Soo;Kim, Young-Jun;Shin, Yong-Kyoo;Lee, Kwang-Soo
    • The Korean Journal of Pharmacology
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    • v.29 no.1
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    • pp.107-120
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    • 1993
  • Particulate or soluble stimuli appear to stimulate phagocytic cell's response by the change of $Ca^{2+}$ mobilization and by the activation of protein kinase C. In contrast, it is reported that activation of protein kinase C could attenuate agonist-stimulated elevation of $Ca^{2+}i$ in neutrophils. PAF elicited an increase of $Ca^{2+}i$ in peritoneal macrophages in a dose dependent fashion and $Ca^{2+}$ extrusion was accompanied. PAF-induced elevation of $Ca^{2+}i$ was not affected by TMB-8, verapamil and TTX. TEA stimulated PAF-induced mobilization of $Ca^{2+}i$ and delayed lowering of $Ca^{2+}i$. Five mM EGTA almost completely inhibited PAF-induced mobilization of $Ca^{2+}i$. After the addition of PAF, membrane permeability was markedly increased up to 5 min and then slowly increased. PAF-induced LDH release was slightly decreased by EGTA plus TMB-8. PAF-stimulated superoxide generation was inhibited by EGTA, TMB-8 and verapamil but not affected by TTX and TEA. PAF-induced elevation of $Ca^{2+}i$, increased membrane permeability and superoxide generation were inhibited by IQSP, chlorpromazine and propranolol. PAF-induced LDH release was significantly inhibited by chlorpromazine and minimally decreased by propranolol. After the pretreatment with PMA, the stimulatory effect of PAF on the elevation of $Ca^{2+}i$ and LDH release in macrophages was significantly decreased. These results suggest that PAF may exert the stimulatory action on peritoneal macrophages of mouse by the elevation of $Ca^{2+}i$ and by the activation of protein kinase C. Preactivation of protein kinase C appears to attenuate the stimulatory action of PAF on macrophage response.

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Quality stability of anchovy-based powder for instant soup packed in tea bag (탈산소제에 의한 Tea bag 포장한 멸치 복합 분말의 저장 안정성)

  • Lee, Ho-Yeon;Chung, Bu-Gil;Son, Kwang-Tae;Joo, Dong-Sik;Kim, Jin-Soo;Lee, Eung-Ho
    • Applied Biological Chemistry
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    • v.36 no.5
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    • pp.321-325
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    • 1993
  • The anchovy-based powder for instant soup packed in twenty tea bag were repacked with (Product B) or without oxygen absorber (Product A) in laminated film bag $(PVDC/OPP,\;thickness:\;100.5\;{\mu}m,\;size:22{\times}18cm)$, and then stored at ambient temperature $(25{\pm}3^{\circ}C)$. Moisture, crude protein and crude lipid contents in anchovy-based powder for instant soup were 12.1%, 54.7% and 2.9%, respectively. Moisture content showed little changes during storage in both product (A) and (B). pH and saturated fatty acid such as 20 : 5 and 22 : 6 decreased, while volatile basic nitrogen content, carbonyl value, thiobarbituric acid value, monoenoic fatty acid such as 16 : 0, brown pigment formation and Hunter values increased during storage of product (A). But, these values showed a little change during storage of product (B). It is concluded that anchovy-based powder for instant soup can be handily and safely used during storage of 150 days.

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Effect of Natural Plant Components on the Nitrite-scavenging (천연식물성분이 아질산염 소거에 미치는 영향)

  • 이수정;정미자;신정혜;성낙주
    • Journal of Food Hygiene and Safety
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    • v.15 no.2
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    • pp.88-94
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    • 2000
  • The purpose of this presents is to investigate, using natural food extracts(Green tea; Camellia sinesis, Du'chung; Eucommia ulmoides Oliver, Eu sung cho; Houttuynia cordate Thunb, Sam back cho; Saurus Chinensis, Baek hwa sa seal oho; Oldenladia diffusa Roxb., Laver; Porphyra tenera, Sea mustard; Undaria pinnatifda and Sea staghorn; Condium fragile) and juices(Sweet pepper; Capsicum annuum var. angulosum, Kale; Brassia oleracea var. acephala, Cucumber; Cucumis sativus, Onion; Allium cepa, Tomato; Lycopericon esculentum, Maesil; Prunus mume, Plum; Prunus salicina and Grape; Vitis spp.), the effect of natural plant components on the nitrite-scavenging under the different levels of pH. From the above mentioned extracts and juices, the content of vitamin C was detected, containing 65.1∼77.1 mg/100g, at the highest level in the Green tea, and followed by vegetables and fruits in order. The nitrite scavenging effect of teas, medicinal plants, and seaweed extracts, in the reaction system under the condition of pH 1.2, were 57.0∼100%, 50.0∼100%, and 18.0∼99.0%, respectively. Especially, the nitrite was scavenged to the level of 100%1 when 10 ml of kale and 5 ml of maesil Juice

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Reaction Optimization for Enzymatic Synthesis of Astragalin (효소를 이용한 아스트라갈린 합성 반응의 최적화)

  • Lee, Seul Bi;Chung, Dae-won
    • Applied Chemistry for Engineering
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    • v.23 no.4
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    • pp.394-398
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    • 2012
  • Astragalin (AS), kaempferol monoglycoside, is classified as a polyphenol, and a minute quantity of AS is known to be present in several plants. Recently, it was reported that AS can be prepared by the partial hydrolysis of camelliaside A (CamA) and camelliaside B (CamB) in the tea seed extract (TSE) in the presence of a commercial enzyme complex such as Mash. In this paper, the effects of reaction temperature, amount of enzyme, and the substrate concentration on the reactivity were investigated. As the reaction temperature or the amount of enzyme increased, the reaction rate to produce AS increased, however, the hydrolysis of AS into KR was also enhanced. As a conclusion, the reaction, when 2 mL of Mash to 1 g of TSE was applied with a substrate concentration of 15% at $50^{\circ}C$, was found to be optimum, based on the reaction rate and the selectivity to AS.

Effects of Coagulants on the Manufacturing of Soybean Curd Containing Natural Materials (응고제에 따른 천연물 첨가두부의 제조 특성)

  • Choi, You-One;Chung, Hun-Sik;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.7 no.3
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    • pp.249-255
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    • 2000
  • Soybean curd has been known as one of the most favorite traditional Korean foods as well as being high in protein. Each concentration of added natural materials soybean curds was chosen as 4% of carrot, 10% of cucumber, 1.0% of spinach and 0.05% of green tea powder. The yield of soybean curd containing natural materials was similar to that of non-containing curd. According to add GDL as coagulant, the yield of soybean curd containing natural materials was the highest. The turbidity of added natural material soybean curds was the highest coagulated with $CaCl_2$, but soybean curd containing green tea had the highest turbidity in the coagulated with GDL. In the chromaticity and texture properties of the additive natural materials in yhe soybean curd, the variety of additives had no effect. In the composition of natural materials, the carotenoid and chlorophyll content of soybean curds were high with $MgCl_2$ and $CaCl_2$ but polyphenol was high use of $CaSo_4$ and GDL.

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Inhibitory Effect of Flavan-3-ols Isolated from Oolong Tea on Xanthine Oxidase (우롱차로부터 분리된 Flavan-3-ol 화합물의 Xanthine Oxidase에 대한 저해 영향)

  • An, Bong-Jeun;Bae, Man-Jong;Choi, Chung
    • Korean Journal of Food Science and Technology
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    • v.28 no.6
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    • pp.1084-1088
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    • 1996
  • As a series of study for an anti-gout agent, two flavan-3-ols have been isolated from Oolong tea. They showed positive reaction in $anisaldehyde-H_2SO_4$ solution and $FeCl_3$, which were able to be confirmed in TLC with dimeric flavan-3-ols. The components were identified as procyanidin B-2 and procyanidin B-2-O-3, 3'-di-gallate by spectroscopic analysis. Their inhibitory effect on xanthine oxidase was also investigated and procyanidin B-2-O-3, 3'-digallate showed 60.6% inhibition at $50\;{\mu}mole$. The compound competitively inhibited the xantine oxidase and dimeric flavan-3-ols contanining gallate had higher inhibition activity.

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In vitro antioxidant activity of black tea (Camellia sinensis L.) residue extract (홍차박 추출물의 in vitro 항산화 활성)

  • Kim, Dong Chung
    • Journal of Applied Biological Chemistry
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    • v.62 no.3
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    • pp.281-286
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    • 2019
  • A black tea (Camellia sinensis L.) residue extract (BTRE) was prepared by 30% ethanol extraction to evaluate its in vitro antioxidant activity. The yield and polyphenol content of BTRE were $22.4{\pm}1.18%$ and $23.2{\pm}1.02{\mu}g$ gallic acid equivalents/mg-extract, respectively. Antioxidant activity of BTRE proportionally increased as BTRE concentration increased. $IC_{50}$ values of BTRE for cation radical, free radical and nitrite scavenging were 141.8, 108.1, and $397.2{\mu}g/mL$, respectively. Also $IC_{50}$ value of BTRE for ferric reducing anti-oxidant power was $97.8{\mu}g/mL$. BTRE effectively inhibited linoleic acid peroxidation. These results imply that BTRE possessed potent antioxidant activity, thus being utilized as a physiologically active material.

Design of A Noise Controller for A Linear system using the CDM (CDM 방법을 사용한 선형시스템의 신뢰성 있는 소음제어기 설계)

  • Kim, Jung-Whan;Chung, Tea-Jin;Lee, Sang-Cheol;Jeong, Yang-Woong;Chung, Chan-Soo
    • Proceedings of the KIEE Conference
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    • 1998.07b
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    • pp.455-457
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    • 1998
  • This paper designs a noise controller for the small cavity using Coefficient Diagram Method(CDM). In the small cavity system, there exist nonlinear characteristics such as uncertain-time delay and parameter variation. In the controller design of nonlinear system with uncertainty need to the higher order controller or complexity computation. The coefficient diagram is convenient implementation of the control system design method, that is utilized as a vehicle to collectively express the important features of the system and an improved version Kessler's standard form and the Lipatov stability condition of a constitutes the theoretical basis. Simultaneously, it is provided a desired specification, such as the robustness, the stability, faster response, and lower order controller. A simulation of the system with the proposed controller shows sufficient noise cancelation in small cavity.

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