• 대한방사선기술학회지:방사선기술과학
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• 제42권5호
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• pp.345-350
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• 2019

Liver biopsy is the gold standard for diagnosing liver fibrosis, but it is invasive and has a risk for complications. For this reason, recently, study has been actively conducted on non-invasive liver fibrosis evaluation method. But, there is no established standard for the type of diffuse liver disease. Therefore, this study was suggest the usefulness and cut-off values of Fibroscan, FIB-4, APRI and AAR of patients with hepatitis C in Korea. According to the diagnosis, 240 people in hepatitis C are classified into fatty liver, chronic hepatitis, and liver cirrhosis. The statistical analysis was performed by ANOVA to verify difference between groups. The ROC curve was analyzed to determine the usefulness and practical cut-off value. As a result, for all diseases, the AUC value for Fibroscan was 0.8 over and the APRI was 0.7 over. Cut-off value of serum based liver fibrosis markers was increased in order of fatty liver, chronic hepatitis and liver cirrhosis. If Fibroscan and serological liver fibrosis markers are applied to predict liver fibrosis, it is expected that excessive liver biopsy can be reduced.

• Journal of Korean Biological Nursing Science
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• 제8권2호
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• pp.41-59
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• 2006

Chronic liver diseases and hepatic cancer have been reported as 10% of cause of death in Koreans. Regardless of various causes, chronic liver disease accompanies commonly hepatic fibrosis. But still the mechanism of hepatic fibrosis remains poorly understood. Using the dimethylnitrosamine(DMN)-induced hepatic fibrosis rat model, We performed to evaluate the possible therapeutic effect of RIP(extracts of Phellodendron amurense and Patrinia scabiosaefolia) and to investigate the changes in referential connective tissue proteins($TGF-{\beta}_1$, ${\alpha}$-smooth muscle actin, and vimentin) as a marker of fibrogenesis. For these purposes, liver tissues were stained with H & E, and Azan staining for estimation of developing fibrosis. In the DMN-treated rat liver tissue, fibrosis were developed forming incomplete septal fibrosis. Whereas, in the RIP-treated rat liver tissues, the fibrosis were decreased recovering to normal morphology. The expressions of $TGF-{\beta}_1$, ${\alpha}$-smooth muscle actin($\alpha-SMA$), and vimetin were increased in the DMN-treated rat liver tissues, but decreased in the various areas of RIP-treated rat liver tissues. According to these results, RIP could be a possible therapeutic agent to reduce hepatic fibrosis, and the $TGF-{\beta}_1$, ${\alpha}$-SMA, and vimentin could be possible indicative markers of hepatic fibrosis development and recovery.

• 한국방사선학회논문지
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• 제13권4호
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• pp.539-546
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• 2019

간조직 생검은 침습적이며, 합병증의 위험을 동반함에도 불구하고 간섬유화의 정도를 예측하는 표준 진단법으로 적용된다. 이러한 단점을 보완하기 위해 본 연구에서는 만성 C형간염 환자 200명을 대상으로 Fibroscan(R)을 이용하여 간섬유화 등급을 나누고, ROC 곡선을 측정하여 혈청학적검사로 계산되는 FIB-4, APRI, AAR의 유용성과 실질적인 Cut-off value를 알아보고자 하였다. 그 결과 간섬유화 평가를 위해 AAR을 적용하는 것보다는 APRI, FIB-4를 이용하는 것이 적절할 것으로 생각되며, 경한 섬유화 등급을 예측하기 위해서는 APRI, 간경변군인 F4등급에는 FIB-4를 사용하는 것이 유용하다고 판단된다. 혈청학적 간섬유화 표지자의 간편하고 반복 측정이 가능하다는 장점을 이용해 간 섬유화의 경과 관찰 기간을 줄일 수 있으므로 나아가 간경변과 간암의 유병률을 감소시킬 수 있을 것으로 사료된다.

• 스마트미디어저널
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• 제2권2호
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• pp.33-38
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• 2013

본 논문에서는 급성 알코올성 간 손상과 만성 에탄올성 간섬유증이 유도된 마우스로부터 획득한 라만 스펙트럼에서 배경 잡음을 제거하기 위한 기준선 추정 방법을 조사하였다. 기준선을 추정하기 위해 일차 미분, 선형계획법, rolling ball을 이용한 방법을 적용하였다. 각 방법의 적절한 압력 파라미터를 MAP(maximum a posteriori probability)의 훈련율에 의해 결정하였다. 실험 절과에 따르면 rolling ball 알고리즘을 이용한 기준선 추정 방법이 급성 알코올성 간 손상과 만성 에탄올성 간섬유증의 MAP 분류에서 평균 89.4%로 가장 좋은 결과를 나타냈다. 이 결과로부터 라만 스펙트럼의 기준선 추정에 적절한 방법과 파라미터를 결정하는 것이 분류 성능에 미치는 영향을 확인하였다.

• Journal of Ginseng Research
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• 제44권3호
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• pp.386-398
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• 2020

Tissue fibrosis is an eventual pathologic change of numerous chronic illnesses, which is characterized by resident fibroblasts differentiation into myofibroblasts during inflammation, coupled with excessive extracellular matrix deposition in tissues, ultimately leading to failure of normal organ function. Now, there are many mechanistic insights into the pathogenesis of tissue fibrosis, which facilitate the discovery of effective antifibrotic drugs. Moreover, many chronic diseases remain a significant clinical unmet need. For the past five years, many research works have undoubtedly addressed the functional dependency of ginsenosides in different types of fibrosis and the successful remission in various animal models treated with ginsenosides. Caveolin-1, interleukin, thrombospondin-1 (TSP-1), liver X receptors (LXRs), Nrf2, microRNA-27b, PPARδ-STAT3, liver kinase B1 (LKB1)-AMPK, and TGF-β1/Smads are potential therapy targeting using ginsenosides. Ginsenosides can play a targeting role and suppress chronic inflammatory response, collagen deposition, and epitheliale-mesenchymal transition (EMT), as well as myofibroblast activation to attenuate fibrosis. In this report, our aim was to focus on the therapeutic prospects of ginsenosides in fibrosis-related human diseases making use of results acquired from various animal models. These findings should provide important therapeutic clues and strategies for the exploration of new drugs for fibrosis treatment.

• 대한방사선기술학회지:방사선기술과학
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• 제42권3호
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• pp.187-194
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• 2019

The purpose of this study was to evaluate the pathologic results of hepatic parenchyma parameters such as liver parenchyma, liver surface, liver margin and liver, portal vein, spleen size, And to evaluate the usefulness of fibrosis progression and hepatic ultrasonography. The sensitivity, specificity, positive predictive value, and prognostic value according to the stage of fibrosis and grade of inflammation were divided into two groups according to the morphologic variable "A" through ultrasound and "B" We evaluated the predictive value and predicted the variables to evaluate fibrosis in clinical diagnosis and treatment of patients with chronic liver disease. The sensitivity and specificity of hepatic fibrosis in hepatic morphologic variables and other size variables were highest in liver surface and edge. The morphologic parameters used in the evaluation of fibrosis were clinically relevant in distinguishing the fibrosis stage from the results of liver biopsy.

• BMB Reports
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• 제53권6호
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• pp.317-322
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• 2020

Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases. NAFLD can further progress to irreversible liver failure such as non-alcoholic steatohepatitis (NASH) fibrosis and cirrhosis. However, specific regulator of NASH-fibrosis has yet to be established. Here, we found that glutathione peroxidase 7 (GPx7) was markedly expressed in NASH fibrosis. Although GPx7 is an antioxidant enzyme protecting other organs, whether GPx7 plays a role in NASH fibrosis has yet to be studied. We found that knockdown of GPx7 in transforming growth factor-β (TGF-β) and free fatty acids (FFA)-treated LX-2 cells elevated the expression of pro-fibrotic and pro-inflammatory genes and collagen synthesis. Consistently, GPx7 overexpression in LX-2 cells led to the suppression of ROS production and reduced the expression of pro-fibrotic and pro-inflammatory genes. Further, NASH fibrosis induced by choline-deficient amino acid defined, high fat diet (CDAHFD) feeding was significantly accelerated by knockdown of GPx7, as evidenced by up-regulated liver fibrosis and inflammation compared with CDAHFD control mice. Collectively, these results suggest that GPx7 might be a novel therapeutic target to prevent the progression and development of NAFLD.

• 한국방사선학회논문지
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• 제17권7호
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• pp.1057-1065
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• 2023

비침습적 간섬유화 진단은 만성 간질환 환자들에게 매우 중요한 사항이다. 많은 환자들에게 간조직검사를 시행할 수가 없기 때문에 의미 있는 간섬유화 정도를 조기에 예측함으로써 만성 간질환의 치료와 간세포암 및 간경변증으로 인한 합병증을 감소시킬 수 있다. 본 연구는 비침습적인 간섬유화 검사 방법 중 간초음파 소견과 함께 전단파 탄성 초음파를 이용하여 간섬유화의 정량적 측정과 간탄성도 수치와 연관성을 갖는 혈청학적 인자도 비교 분석하였다(p<0.05). 그 결과 정상 집단의 횡파탄성측정값은 4.55 ± 0.69 kPa였으며, 에코 패턴의 비정상 대조군은 8.27 ± 1.83 kPa로 B형 간염 보균 집단에서 전단파 탄성 초음파 측정값이 높게 나타났으며, 혈청학적 인자들 중 AST, ALT, GGT, PT가 SWE 범주에 따른 섬유화의 정도에 따라 통계학적으로 유의한 양의 연관성을 보였으나(p<0.05) ALP와 TB는 통계적으로 유의미한 차이를 나타내지 않았다(p=0.163, p=0.567). 반면에 Albumin과 PLT는 유의한 음의 연관성을 보였다(p<0.05). 임상적으로 간경변 증후가 없는 만성 B형 간염 환자들의 간섬유화 추적 관찰 및 반복 검사 시 간초음파와 전단파 탄성 초음파 검사를 통한 탄성도 값을 추가로 활용한다면 검사자 간 객관적 진단에 있어 도움이될 것이며, 일차적인 진료에 유용할 것으로 사료된다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.155.2-156
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• 2003

The adducts of lipid peroxidation and related aldehydic end are mediators of chronic poisoning and affect the development of chronic liver damage leading to fibrosis and cirrhosis. Substances delayed or suppresed lipid peroxidation could have an antioxidant and protective effect in liver disease. In this study, it was attempted to find out above mentioned effect of Solanum lycopersicum investigated in CCl4 induce liver fibrosis model. (omitted)

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9423-9426
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• 2014

Objective: Hapatitis B visus (CHB)-induced fibrosis is a precancerous condition of liver. To explore the influence of Chongcao Preparation (Chongcao Yigan Capsule) on the function of intestinal flora and chemoprevention for patients with CHB-induced liver fibrosis. Methods: A total of 136 patients with CHB-induced liver fibrosis were randomly divided into control group treated with lamivudine (LAM) and research group added with Chongcao Yigan Capsule for totally 48 weeks. The changes of intestinal flora, secretory immunoglobin A (SIgA), serum albumin (ALB), prealbumin (PALB), IgA and IgG at different time points in both groups were observed. Results: Before treatment, there was no significant difference between two groups in each index (P>0.05). After treatment, the intestinal flora were evidently optimized in research group than treatment before (P<0.05 or P<0.01), and were apparently better than those in control group (P<0.05 or P<0.01); SIgA was obviously increased and ALB, PALB, IgA and IgG were markedly improved in research group than treatment before (P<0.05 or P<0.01), and were significantly better than those in control group (P<0.05 or P<0.01). Conclusions: Chongcao Yigan Capsule could regulate the intestinal flora, increase SIgA, serum ALB and PALB concentrations and significantly improve serum IgA and IgG as well as strengthen the immunological function and autologous repair capacity of patients with CHB-induced liver fibrosis.