• ALGAE
• /
• v.30 no.4
• /
• pp.291-301
• /
• 2015

The present study described with different microscopy approaches chloroplasts lobes in Laurencia sensu latu (Rhodophyta) species and found inter-specific differences among them. Chloroplasts were investigated using confocal laser scanning microscopy (LSM), transmission electron microscopy (TEM) and high resolution scanning electron microscopy (HRSEM). Using and TEM and HRSEM images we distinguished chloroplasts with lobes than chloroplasts without lobes in Yuzurua poiteaui var. gemmifera (Harvey) M. J. Wynne and Laurencia dendroidea J. Agardh cortical cells. The LSM images showed chloroplasts lobes (CLs) with different morphologies, varying from thicker and longer undulated projections in Y. poiteaui var. and L. dendroidea to very small and thin tubules as in Laurencia translucida Fujii & Cordeiro-Marino. The diameter and length of CLs from Y. poiteaui var. and L. dendroidea were significantly higher than L. translucida CLs (p < 0.01). Based on LSM observations, we suggest that lobes morphology has a taxonomic validity only to characterize L. translucida species.

• Korean Journal of Ecology and Environment
• /
• v.34 no.3 s.95
• /
• pp.185-191
• /
• 2001

The morphological variation and density of the Euglena viridis cells and environmental factors of urban waterways of Daejoncheon , Jeonjucheon, Kwangjucheon, Kumhogang, Mihocheon,and Musimcheon, Korea were studied from 25December, 1995 to 5 January, 1997 in order toelucidate possible relationships among the bio-logical and abiological factors. All E. viridis cells were same in having single star-cluster of chlo-roplast lobes and included two morphotypes based on other detailed morphology. The morphotype I cells agreed well with the typical form off. viridrs and commonly occurred in most of waters and bloomed with $5386\;cells\;{\cdot}\;mL^{-1}$ in Kwangjucheon. The density of the morphotype Ipositively correlated with ammonium (r = 0.80)and nitrite (r = 0.68), while negatively with nit-rate concentration. The morphotype II cells were characterized by having randomly scattered cytoplasmic granules beneath pellicle and unevenmargined lobes of chloroplasts. The density of the morphotype II positively correlated with nitrate (r = 0.98), while negatively correlated with ammonium and nitrite. However, the density of each morphotype was not significantly related with inorganic phosphate, temperature and pH of surface water. These results indicate that E. viridis includes two morphotypes in urban waterways in Korea, that coexist in the same period and station as a response of allocation of nitrogenous nutrients.

• Korean Journal of Environmental Biology
• /
• v.37 no.2
• /
• pp.119-128
• /
• 2019

The morphological characteristics of the bloom-forming dinoflagellate Akashiwo sanguinea isolated from Jangmok Harbour, Geoje in Korea was examined using light and scanning electron microscope (SEM), and its large subunit (LSU) rDNA was sequenced. Additionally, investigation was done on the effects of temperature and salinity on the growth of A. sanguinea. The cells were dorso-ventrally compressed up to 54.7-70.3 ㎛ long and 31.5-48.5 ㎛ wide. The epicone was conical while the hypocone was separated into two lobes. The nucleus was positioned at the center of the cell. The yellow-brown chloroplasts radiated close to the cell center. SEM observation indicated that A. sanguinea has an e-shaped apical groove. Molecular phylogeny based on LSU rDNA gene sequences revealed that the A. sanguinea strains isolated from Jangmok Harbor were classified in the clade of ribotype A. The maximum growth rate (0.50 day^-1) was observed at 20℃ and 20 psu, while the maximum cell density (1,372 cells mL^-1) was observed at 25℃ and 30 psu. This indicates that the blooms of A. sanguinea ribotype A in Korean coastal area are affected by water temperature, rather than the salinity.