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http://dx.doi.org/10.11626/KJEB.2019.37.2.119

Bloom-forming dinoflagellate Akashiwo sanguinea(Dinophyceae) in Jangmok Harbour of Geoje Island, Korea: Morphology, phylogeny and effects of temperature and salinity on growth  

Han, Kyong Ha (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
Li, Zhun (Research Center for Fishery Resource Management Based on the ICT, Chonnam National University)
Youn, Joo Yeon (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
Kang, Byeong Jun (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
Kim, Hyun Jung (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
Seo, Min Ho (Marine Ecology Research Center)
Soh, Ho Young (Research Center for Fishery Resource Management Based on the ICT, Chonnam National University)
Shin, Hyeon Ho (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
Publication Information
Korean Journal of Environmental Biology / v.37, no.2, 2019 , pp. 119-128 More about this Journal
Abstract
The morphological characteristics of the bloom-forming dinoflagellate Akashiwo sanguinea isolated from Jangmok Harbour, Geoje in Korea was examined using light and scanning electron microscope (SEM), and its large subunit (LSU) rDNA was sequenced. Additionally, investigation was done on the effects of temperature and salinity on the growth of A. sanguinea. The cells were dorso-ventrally compressed up to 54.7-70.3 ㎛ long and 31.5-48.5 ㎛ wide. The epicone was conical while the hypocone was separated into two lobes. The nucleus was positioned at the center of the cell. The yellow-brown chloroplasts radiated close to the cell center. SEM observation indicated that A. sanguinea has an e-shaped apical groove. Molecular phylogeny based on LSU rDNA gene sequences revealed that the A. sanguinea strains isolated from Jangmok Harbor were classified in the clade of ribotype A. The maximum growth rate (0.50 day-1) was observed at 20℃ and 20 psu, while the maximum cell density (1,372 cells mL-1) was observed at 25℃ and 30 psu. This indicates that the blooms of A. sanguinea ribotype A in Korean coastal area are affected by water temperature, rather than the salinity.
Keywords
apical groove; cell density; growth rate; LSU rDNA; ribotype; SEM;
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1 Baek SH and HM Joo. 2012. Relationships between cell bio-volume and growth rate of dominant red tide organisms in the coastal water. Korean J. Environ. Biol. 30:1-8.
2 Botes L, AJ Smit and PA Cook. 2003. The potential threat of algal blooms to the abalone (Haliotis midae) mariculture industry situated around the South African coast. Harmful Algae 2:247-259.   DOI
3 Chen T, Y Liu, S Song, C Li, YZ Tang and Z Yu. 2015. The effects of major environmental factors and nutrient limitation on growth and encystment of planktonic dinoflagellate Akashiwo sanguinea. Harmful Algae 46:62-70.   DOI
4 Cho SY, JS Ki and MS Han. 2008. Morphological characteristics and molecular phylogeny of five unarmored dinoflagellates in Korean Coastal Waters. Algae 23:15-29.   DOI
5 Darriba D, GL Taboada, R Doallo and D Posada. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat. Methods 9:772.
6 Doucette GJ and PJ Harrison. 1990. Some effects of iron and nitrogen stress on the red tide dinoflagellate Gymnodinium sanguineum. Mar. Ecol. Prog. Ser. 62:293-306.   DOI
7 Guillard RRL. 1973. Division rates. p. 472. In Handbook of Phycological Methods: Culture Methods and Growth Measurements, Stein JR (ed.). Cambridge University Press. Cambridge.
8 Hao YJ, DL Tang, L Yu and QG Xing. 2011. Nutrient and chlorophyll-A anomaly in red-tide periods of 2003-2008 in Sishili Bay, China. Chin. J. Oceanol. Limnol. 29:664-673.   DOI
9 Hirasaka K. 1922. On a case of discoloured sea water. Annot. Zool. Japan 10:161-164.
10 Jessup DA, MA Miller, JP Ryan, HM Nevins, HA Kerkering, A Mekebri, DB Cran, TA Johnson and RM Kudela. 2009. Mass stranding of marine birds caused by a surfactant-producing red tide. PLoS One 4:e5540.   DOI
11 Juhl AR, V Velazquez and MI Latz. 2000. Effect of growth conditions on flow-induced inhibition of population growth of a red-tide dinoflagellate. Limnol. Oceanogr. 45:905-915.   DOI
12 Kogame K, T Horiguchi and M Masuda. 1999. Phylogeny of the order Scytosiphonales (Phaeophyceae) based on DNA sequences of rbcL, partial rbcS, and partial LSU rDNA. Phycologia 38:496-502.   DOI
13 Luo Z, W Yang, CP Leaw, V Pospelova, G Bilien, GR Liow, PT Lim and H Gu. 2017. Cryptic diversity within the harmful dinoflagellate Akashiwo sanguinea in coastal Chinese waters is related to differentiated ecological niches. Harmful Algae 66:88-96.   DOI
14 Kumar S, G Stecher and K Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33:1870-1874.   DOI
15 Kwon HK and SJ Oh. 2014. Growth response of the Dinoflagellate Akashiwo sanguinea in relation to temperature, salinity and irradiance, and its advantage in species succession. Korean Soc. Mar. Environ. Saf. 20:1-10.   DOI
16 Lee CK, OH Lee and SG Lee. 2005. Impacts of temperature, salinity and irradiance on the growth of ten harmful algal bloom-forming microalgae isolated in Korean Coastal waters. The Sea 10:79-91.
17 Matsubara T, S Nagasoe, Y Yamasaki, T Shikata, Y Shimasaki, Y Oshima and T Honjo. 2007. Effects of temperature, salinity, and irradiance on the growth of the dinoflagellate Akashiwo sanguinea. J. Exp. Mar. Biol. Ecol. 342:226-230.   DOI
18 Matsubara T, S Nagasoe, Y Yamasaki, T Shikata, Y Shimasaki, Y Oshima and T Honjo. 2008. Inhibitory effects of centric diatoms on the growth of the dinoflagellate Akashiwo sanguinea. Nippon Suisan Gakkaishi 74:598-606.   DOI
19 Stamatakis A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-1313.   DOI
20 Steidinger KA. 1998. Bloom dynamics and physiology of Gymnodinium breve with emphasis on the Gulf of Mexico. pp. 135-153. In Physiological Ecology of Harmful Algal Blooms. Springer, New York.
21 Wong GTF, GC Gong, KK Liu and SC Pai. 1998. 'Excess nitrate' in the East China Sea. Estuar. Coast. Shelf Sci. 46:411-418.   DOI
22 Ronquist F and JP Huelsenbeck. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572-1574.   DOI
23 Wu YL, CX Zhou, YS Zhang, XM Pu and WH Li. 2001. Evolution and causes of formation of Gymnodinium sanguinea bloom in Yantai Sishili Bay. Oceanol. Limnol. Sin. 32:159-167 (with English abstract).   DOI
24 Yamaguchi A and T Horiguchi. 2005. Molecular phylogenetic study of the heterotrophic dinoflagellate genus Protoperidinium (Dinophyceae) inferred from small subunit rRNA gene sequences. Phycol. Res. 53:30-42.   DOI
25 Yu L and YJ Hao. 2009. Process analysis for harmful bloom of Akashiwo sanguinea in Sishili Bay of Yantai. Adv. Mar. Sci. 27:516-522 (with English abstract).   DOI