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http://dx.doi.org/10.4490/algae.2015.30.4.291

Chloroplasts morphology investigation with diverse microscopy approaches and inter-specific variation in Laurencia species (Rhodophyta)  

Paradas, Wladimir Costa (Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (IPJBRJ))
Andrade, Leonardo Rodrigues (Laboratorio de Biomineralizacao, Instituto de Ciencias Biomedicas, CCS, Universidade Federal do Rio de Janeiro)
Salgado, Leonardo Tavares (Instituto de Pesquisas Jardim Botanico do Rio de Janeiro (IPJBRJ))
Collado-Vides, Ligia (Department of Biological Sciences and Southeast Environmental Research Center, Florida International University)
Pereira, Renato Crespo (Departamento de Biologia Marinha, Universidade Federal Fluminense)
Amado-Filho, Gilberto Menezes (Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (IPJBRJ))
Publication Information
ALGAE / v.30, no.4, 2015 , pp. 291-301 More about this Journal
Abstract
The present study described with different microscopy approaches chloroplasts lobes in Laurencia sensu latu (Rhodophyta) species and found inter-specific differences among them. Chloroplasts were investigated using confocal laser scanning microscopy (LSM), transmission electron microscopy (TEM) and high resolution scanning electron microscopy (HRSEM). Using and TEM and HRSEM images we distinguished chloroplasts with lobes than chloroplasts without lobes in Yuzurua poiteaui var. gemmifera (Harvey) M. J. Wynne and Laurencia dendroidea J. Agardh cortical cells. The LSM images showed chloroplasts lobes (CLs) with different morphologies, varying from thicker and longer undulated projections in Y. poiteaui var. and L. dendroidea to very small and thin tubules as in Laurencia translucida Fujii & Cordeiro-Marino. The diameter and length of CLs from Y. poiteaui var. and L. dendroidea were significantly higher than L. translucida CLs (p < 0.01). Based on LSM observations, we suggest that lobes morphology has a taxonomic validity only to characterize L. translucida species.
Keywords
chloroplasts lobes; confocal laser scanning microscopy; Laurencia dendroidea; Laurencia translucida; morphometry; transmission electron microscopy; Yuzurua poiteaui var. gemmifera;
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