• Journal of the Korean Applied Science and Technology
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• v.37 no.2
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• pp.153-161
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• 2020

Arthrospira platensis is the oldest marine microalgae on the planet, is said to contain most of the nutrients needed by the human body. It's components are reported to contain a large amount of various substances such as phycocyanin, chlorophyll and β-carotene, and are known to have an aging and whitening effect. In this study, UVB-induced reactive oxygen species reduction efficacy and antioxidant activity of spirulina purified water extract were investigated. effect was confirmed by measuring DPPH radical scavenging activity, FRAP reducing power and ABTS⁺ radical scavenging activity of 0.05, 0.10, 0.50 1.0 mg/mL of spirulina purified water extract. The coagulation rate, hatching rate and heart rate toxicity were measured by treating spirulina purified water extract with 0.05, 0.10, 0.50 mg/mL concentration using Zebrafish, an alternative experimental animal model. UVB-induced ROS measurement was treated with spirulina extract at 0.05, 0.10, 0.50 mg/mL concentration, and then stained with DCFH-DA to confirm the inhibitory effect of ROS. As a result of measuring antioxidant effect, DPPH, FRAP and ABTS⁺ showed concentration-dependent antioxidant effects in comparison with ascorbic acid. and measuring the coagulation rate, hatching rate, and heart rate using Zebrafish, an alternative experimental animal, it was confirmed that there was no toxicity in 0.05 and 0.10 mg/mL except 0.5 mg/mL compared to the control group. The ROS scavenging activity of UVB-induced zebrafish showed higher ROS reduction than the positive control. The results of this study suggest that spirulina and purified water extracts are valuable for UV and skin protection cosmetics.

• Horticultural Science & Technology
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• v.35 no.2
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• pp.188-198
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• 2017

Asparagus (Asparagus officinalis L.) spears were treated with white (color temperature 4,500 k), blue (peak 450 nm), and red (peak 660 nm) light-emitting diodes (LEDs) at a photosynthetic photon flux density (PPFD) of $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for 12 h, and UV-B (280 nm) at 0.5 kJ or 1.0 kJ to determine the effect on agronomic characteristics, antioxidant phytochemicals, and antioxidant activity. The fresh weight, length, and width of spears were not affected by light quality treatments. The free sugars and chlorophyll contents were increased by 9 and 41%, respectively in the UV-0.5 kJ treatments. Among the antioxidant phytochemicals (vitamin C, total phenol, rutin, and total flavonoid), vitamin C was most greatly affected by the light treatments. Vitamin C content was significantly increased in asparagus spears subjected to the white (114%), red (137%), and UV-0.5 kJ(127%) treatments compared to the control. By contrast, rutin, total phenol, and total flavonoid content were increased only in samples subjected to the red and UV-0.5 kJ treatment. Furthermore, antioxidant activity, as measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, increased in white, red, and UV-0.5 kJ treatments by about 43, 41, and 43%, respectively, compared to the control. These results suggest that postharvest treatment of asparagus spears with red light at $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for 12 h or with UV-B (280 nm) at 0.5 kJ could enhance the functional quality of the asparagus spears by increasing the content of phytochemicals like vitamin C, rutin, total phenolics, and total flavonoids.

• Journal of Environmental Impact Assessment
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• v.21 no.3
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• pp.483-501
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• 2012

The purpose of this study was to apply a multi-metric model of Lentic Ecosystem Health Assessments(LEHA) for environmental impact assessments of Cheongpyung Reservoir during 2005 - 2006 and assessed the ecological model values. The ecosystem model of LEHA was composed of eleven metrics such as biological parameters($B_p$), physical parameters($P_p$), and chemical parameters($C_p$), and determined the rank of ecological health by the criteria. The variables of $B_p$ were metrics of % sensitive species($M_2$, NMS) and insectivore species($M_5$, % $I_n$), which decrease as the water quality degradates, and these metric values were low as 1.5% and 32.4%, respectively. In contrast, the proportions of tolerant species and omnivore species as the other $B_p$ parameters were 43% and 62%, respectively, which indicate a degradation and disturbance of the ecosystem. Riparian vegetation coverage($M_9$, % $V_c$) as a variable of $P_p$, were higher in the 2nd than 1st survey, and decreased toward the dam site from the headwaters. This was due to a habitat simplification(modifications) by frequent bottom dredging of sand and rocks. The variables of $C_p$ were two metrics of specific conductivity($M_{10}$, $C_I$) as an indicator of ionic contents(cations and anions) and the Trophic State Index(TSI) based on chlorophyll-a($M_{11}$, $TSI_{CHL}$) as an indicator of trophic state. These metric values of $C_p$ had high temporal variations, but low spatial variations on the main axis of the reservoir along with the ecological health of a good condition. The environmental impact assessments using the LEHA multi-metric model indicated that the model values of LEHA averaged 30.7 in 1st survey(fair - poor condition) vs. 28 in 2nd survey(poor condition), indicating a temporal variation of the ecological health. The model values of LEHA showed a minimum(28) in the lacustrine zone(S5) and ranged from 29 to 30 in the other locations sampled, indicating a low longitudinal variation. Overall, environmental impact assessments, based on LEHA model, suggest that chemical water quality conditions were in good, but biological conditions were disturbed due to habitat modifications by frequent dredgings in the system.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.5
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• pp.709-715
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• 2015

As consumers interest in microgreens is increasing worldwide, the production of leafy microgreens uisng different LED lights was investigated in this study. The experiment was carried out to evaluate the effects of different LED lights on the composition and vitamin C contents of buckwheat microgreens. Physicochemical properties of buckwheat microgreens grown under different lights (red, blue, and white) and control exposed to a dark room were investigated. Moisture contents of buckwheat microgreens were 95.65% under white light (WL), 95.75% under blue light (BL), 90.77% under red light (RL), and 89.71% under dark room (DR). Crude ash contents of buckwheat microgreens grown under WL, DR, RL, and BL were 0.39%, 0.39%, 0.31%, and 0.37%, respectively. Crude protein contents of buckwheat microgreens grown under DR, RL, WL, and BL were 7.12%, 7.81%, 1.60%, and 2.40%, respectively. Crude fat contents of buckwheat microgreens grown under DR, BL, RL, and WL were 1.12%, 0.54%, 0.35%, and 0.22%, respectively. $^{\circ}Brix$ was the highest in microgreens grown under BL and RL and the lowest in buds grown under DR. Vitamin C content was the highest in buds grown under WL and the lowest in buds grown under BL. Total chlorophyll content was the highest in microgreens grown under RL and the lowest in buds grown under WL. For mineral content measurement of buckwheat microgreens, Ca, K, Mg, and P contents were high whereas B, Cu, and Zn contents were not detected. The mineral contents of buckwheat microgreens according to each color of light showed significant differences. These results demonstrated that treatment of different colored LED lights during cultivation was able to increase vitamin C content up to affecting the nutritional value of buckwheat microgreens.

• Korean Journal of Environmental Biology
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• v.20 no.1
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• pp.73-77
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• 2002

The effect of salicylic acid (SA) on C $d^{2+}$ - induced physiological toxicity in Commelina communis was investigated. 3- weeks old Commelina communis was transferred to and grown in Hoagland solution in the presence or absence of 100 $\mu$M C $d^{2+}$ and SA for 3 weeks. In the treatment of C $d^{2+}$ + SA, the length of stem was increased to 0.7 cm for 3 weeks (C $d^{2+}$, 2.1cm; control, 7.2 cm). C $d^{2+}$ + SA reduced total chlorophyll content up to 86%, and changed chlorophyll a/b ratio below 1.6. C $d^{2+}$ + SA also reduced about 40-78% of water potential, but C $d^{2+}$ increased 16-39% from 1 week to 3 weeks. C $d^{2+}$ + SA also inhibited 27% of Fv/Fm, but in case of C $d^{2+}$, Fv/Fm was not changed. The treatment of C $d^{2+}$ + SA showed about 37-58% inhibition of photosynthetic activity when measured at various light intensity (500-1000 $\mu$mol $m^{-2}$ $s^{-1}$ ). In the case of C $d^{2+}$ treatment, photosynthetic activity was inhibited to 12-15%. Similar effect was found in terms of stomatal conductance. Therefore, it could be concluded that the treatment of C $d^{2+}$ + SA into plant decrease or block various physiological activities and lend to die by double effects of both chemicals.cts of both chemicals..

• Journal of Korean Society of Forest Science
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• v.105 no.4
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• pp.510-516
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• 2016

This study was conducted to investigate the physiological responses of newly-germinated seedlings of Fraxinus rhynchophylla Hance, Zelkova serrata (Thunb.) Makino, Betula costata Trautv. and Quercus variabilis Blume to open-field experimental warming. The seedlings in the warmed plots were warmed with $2.7^{\circ}C$ higher air temperature than those in the control plots using infrared heaters since April, 2015. Physiological responses (stomatal conductance, transpiration rate, chlorophyll content and net photosynthetic rate) to experimental warming varied with the species and the time of the measurement. Stomatal conductance ($mmol{\cdot}m^{-2}{\cdot}s^{-1}$) tended to decrease for F. rhynchophylla (Control: $158.97{\pm}42.76$; Warmed: $42.07{\pm}8.24$), Z. serrata (Control: $170.53{\pm}27.22$; Warmed: $101.17{\pm}42.27$) and B. costata (Control: $249.93{\pm}47.39$; Warmed: $150.73{\pm}26.52$). Transpiration rate ($mmol{\cdot}m^{-2}{\cdot}s^{-1}$) also decreased for F. rhynchophylla (Control: $4.08{\pm}0.62$; Warmed: $1.74{\pm}0.39$), Z. serrata (Control: $4.32{\pm}0.44$; Warmed: $3.24{\pm}1.14$) and B. costata (Control: $6.21{\pm}0.38$; Warmed: $4.66{\pm}0.28$). However, warming exhibited only minimal effects on stomatal conductance and transpiration rate of Q. variabilis seedlings. Chlorophyll content increased by the warming treatment while the effect of warming was not significant on net photosynthetic rate, since the warming treatment had a weak influence for changing net photosynthetic rate.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.1
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• pp.135-143
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• 2018

The effect of elevated temperature on temperate paddy rice will be significant for dependable food supply in East Asia. Using temperature gradient field chamber (TGFC), which was designed to make the horizontal air temperature gradient by $0^{\circ}C$ to $3^{\circ}C$ higher than outside, we examined the measurement to understand the effects of extremely high temperature on paddy rice. In particular, the data of the year 2016, the worst heat wave in over 22 years, was analyzed in this study. The rice height in the relatively warmed condition was rapidly increased during early growth stage. However, the average grain weight and number of spikelet per panicle in the warmed chamber condition were gradually declined with increasing air temperature averaged for 40 days after first heading in each chamber. In particular, the grain yield was more dramatically decreased by the raising temperature because the percent ripened grain was quickly dropped as getting over the threshold temperature for pollination. Therefore, the surplus photosynthetic product by such lower grain filling rate may disturbed the decreases of the NDVI (Normalized Difference Vegetation Index) and SPAD chlorophyll values after first (normal) heading. In addition, the late-emerging head grain were appeared. However, this yield was too small to recover the normal yields decreased by extremely high temperature condition. Our result represented that the warmed condition in 2016 would be the critical limit for the stable yield of temperate paddy rice.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.23 no.3
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• pp.143-150
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• 2017

Effects of packaging methods for maintaining the shelf-life and postharvest quality of Korean chive (Allium tuberosum Rotter) usually called buchu in Korea was studied during fall season. Buchu, which has high moisture content and active metabolism after harvest, is characterized by the greater changes in quality during storage and distribution. It is usually packaged in paper box during distribution in domestic channel in Korea where the marketability ends within few days of harvest. In order to maintain the commercial quality of buchu, we tried to use inner film with or without absorbent paper in commercial paper box packaging. The 'Greenbelt' cultivar of buchu was harvested in october and packaged in corrugated paper box (A), in corrugated paper box with micro-perforated high density polyethylene (HDPE) film as inner material (B), a layer of absorbent paper both at the bottom and top of buchu in corrugated paper box (C) and a combination of HDPE film as inner material along with a layer of absorbent paper both at the bottom and top of buchu in corrugated paper box (D), and stored at $5^{\circ}C$ for up to 21 days. Several quality parameters such as fresh weight loss, respiration rate, color, chlorophyll content and sensory qualities were monitored during storage. When buchu was packaged only in paper box, the appearance and quality deteriorated rapidly through decay development thereby sample became unmarketable within 12 days. On the other hand, buchu packaged with inner HDPE film maintained its quality, good visual appearance and exhibited a longer period of marketable life compared to the samples stored in other treatments. However, the use of absorbent paper did not have any significant effect in maintaining quality of buchu. The uses of inner HDPE film was effective in reducing weight loss that resulted better visual appearance of buchu. Results suggest that the use of inner HDPE film in bulk type corrugated paper box packaging and storing at $5^{\circ}C$ could be the optimum condition for commercial storage of buchu. Further investigation on other quality parameters and packaging methods would be useful in maintaining the quality aspects of buchu at postharvest stages.

• Korean Journal of Environmental Agriculture
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• v.12 no.2
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• pp.153-161
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• 1993

This study was conducted to compare the resistance and sensitivity of trees with 6 native species exposing to 4 different levels of $SO_2$gas(0.4, 0.7, 1.5 and 3.0 ppm) respectively. The results are summarized as follows : 1. Visible injuries appeared as spots in the region of intervein on the leaves for all the species and the color of the spots changed from light green and/or brown to light brown, dark brown, and/or redish brown. 2. The sensitivity of the species to $SO_2$ was high in the descending order of Zizyphus jujuba, Cataegus pinnatifida, Viburnum sargentii, Weigela subsessilis, Euonymus japonica, and Acer ginnala. 3. The resistance of the species to $SO_2$ was high in the descending order of Acer ginnala, Eunymus japonica Viburnum sargentii, Weigela subsessilis, Zizyphus jujuba, and Crataegus pinnatifida. 4. When the trees were exposed to $SO_2$gas, the contents of chlorophyll a, b, and a+b were consostently lower than those of control, and water soluble sulfur contents in the leaves were higher than those of control. 5. There was no significant correlation between stomatal resistance and the sensitivity(or resistance) of the trees exposed to $SO_2$ gas. 6. In this study, it was concluded that Acer ginnala was more suitable species than the others for landscape in air polluted area because it showed high resistance, low sensitivity, and low stromal resistance to $SO_2$gas exposure.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.1
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• pp.1-8
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• 2018

The purpose of this study was to investigate the effects of light intensity on the initial growth response of three woody plants for indoor landscaping; Ardisia pusilla, Clusia rosea and Fatsia japonica. The plants were planted in 10cm pots, the light intensities used were of four levels-15, 30, 60, $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD-and light irradiation time was set to 12/12 (day/night). Growth responses including plant height, leaf length, leaf width, chlorophyll fluorescence (Fv/Fm), SPAD and Hunter values were measured at 4-week intervals, and shoot weight and root weight of fresh and dry plants were measured after completion of the experiment. Fatsia japonica tended to show greater leaf length and leaf width as light intensity became greater, while other plants did not show any significant differences at different light intensities. The Fv/Fm value of the Ardisia pusilla was found to be stressed at 60 and $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, while the Fv/Fm values were within normal range with other plants or at other light intensity levels to show no stress. Only Clusia rosea showed significantly different SPAD values at $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and there was no significant SPAD value difference found with other plants or at other light intensity levels. While Hunter values of the Ardisia pusilla did not show any significant differences at any light intensity levels, Clusia rosea and Fatsia japonica showed specificity in L, a and b values at 60 and $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. Ardisia pusilla showed a big stem growth at $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and Clusia rosea showed a steady growth at 60 and $120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$.