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http://dx.doi.org/10.12925/jkocs.2020.37.2.153

Antioxidant Effect of Arthrospira platensis Extract and Effect on UVB-induced Free Radical Production  

Kim, Min Seung (Mokwon University, College of Sciences & Technology, Division of Chemistry & Cosmetics Engineering)
Yang, Jae-Chan (Mokwon University, College of Sciences & Technology, Division of Chemistry & Cosmetics Engineering)
Kim, Bo-Ae (Mokwon University, College of Sciences & Technology, Division of Chemistry & Cosmetics Engineering)
Publication Information
Journal of the Korean Applied Science and Technology / v.37, no.2, 2020 , pp. 153-161 More about this Journal
Abstract
Arthrospira platensis is the oldest marine microalgae on the planet, is said to contain most of the nutrients needed by the human body. It's components are reported to contain a large amount of various substances such as phycocyanin, chlorophyll and β-carotene, and are known to have an aging and whitening effect. In this study, UVB-induced reactive oxygen species reduction efficacy and antioxidant activity of spirulina purified water extract were investigated. effect was confirmed by measuring DPPH radical scavenging activity, FRAP reducing power and ABTS+ radical scavenging activity of 0.05, 0.10, 0.50 1.0 mg/mL of spirulina purified water extract. The coagulation rate, hatching rate and heart rate toxicity were measured by treating spirulina purified water extract with 0.05, 0.10, 0.50 mg/mL concentration using Zebrafish, an alternative experimental animal model. UVB-induced ROS measurement was treated with spirulina extract at 0.05, 0.10, 0.50 mg/mL concentration, and then stained with DCFH-DA to confirm the inhibitory effect of ROS. As a result of measuring antioxidant effect, DPPH, FRAP and ABTS+ showed concentration-dependent antioxidant effects in comparison with ascorbic acid. and measuring the coagulation rate, hatching rate, and heart rate using Zebrafish, an alternative experimental animal, it was confirmed that there was no toxicity in 0.05 and 0.10 mg/mL except 0.5 mg/mL compared to the control group. The ROS scavenging activity of UVB-induced zebrafish showed higher ROS reduction than the positive control. The results of this study suggest that spirulina and purified water extracts are valuable for UV and skin protection cosmetics.
Keywords
Spirulina; Zebrafish; toxicity; Ultarviolet B (UVB); ROS;
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Times Cited By KSCI : 4  (Citation Analysis)
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