• 한국식품조리과학회지
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• 제13권1호
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• pp.56-63
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• 1997

천연물에 존재하는 항균성 물질을 이용하여 천연식품보존제를 개발하고자 한약재 및 민간요법으로 사용되어온 우리나라 전통 향신료인 산초의 항균성을 검색한 결과 클로로포름 분획 에서 B. subtilis, E. coli, L.plantarum 및 S. aureus 등 4종의 균주에 대해 모두 l000 $\mu\textrm{g}$/$m\ell$에서 가장 큰 저해능을 보였으며, 에틸아세테이트 분획 또한 B. sutilis와 E. coli에 대해서 각각 1000 $\mu\textrm{g}$/$m\ell$과 500 $\mu\textrm{g}$/$m\ell$에서 성장이 억제되었다. 가장 큰 활성을 보인 클로로포름층을 다시 분획하여 4그룹으로 나누어 항균성을 검색한 결과 3번 분획에서 4균주에 대해 750 $\mu\textrm{g}$/$m\ell$에서 성장저해 효과를 보였다. chloroform 2차 분획에서 활성이 높게 나타난 subfraction No. 2는 IR, NMR, 그리고 Gchfs를 이용하여 분석한 결과 hexadecanoic acid로 동정되었다.

• 생약학회지
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• 제23권4호
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• pp.264-267
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• 1992

The cytotoxic activity of medicinal plants was screened using A549 human lung cancer cell line. Plant materials were extracted with 80% methanol and fractionated to chloroform and water layers. Each methanol, chloroform, and water extract of thirty-two medicinal plants was tested for cytotoxic activity in A549 cell culture system and the cell viability was measured by SRB assay.

• Natural Product Sciences
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• 제12권1호
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• pp.24-28
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• 2006

The analgesic and the antipyretic activity of the methanol, chloroform, hexane and acetonitrile extracts of the aerial parts of Stachys schimperi Vatke were investigated in mice. The nociceptive response was tested using acetic acid-induced writhing and tail flick method; while hypothermia affect was examined via yeast-induced fever test. The chloroform extract at 500 mg and hexane and acetonitrile extracts at 250 mg produced significant analgesic and antipyretic activity.

• 한국식품과학회지
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• 제31권3호
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• pp.855-863
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• 1999

일반적으로 많이 사용하고 있는 한약재 중 항산화효과가 있는 대상을 선발하였고 그 중 효과가 우수한 건칠을 대상으로 항산화물질을 분리, 효과를 측정하였다. 건칠(Rhus verniciflua STOKES)은 옻나무과(Anacardiaceae)에 속하는 옻나무(Rhus verniciflua STOKES)의 수액으로 한방에서는 구충, 복통, 통경, 변비의 목적으로 사용되어왔다. 옻나무 에탄올추출물은 돈지와 팜유를 기질로 Rancimat test에서 항산화효과가 인정되었으며, 용매의 극성을 각각 달리하여 분획하였을때 chloroform 층에서 항산화 활성이 가장 높았다. 이 분획물을 다시 phenolic compound를 중심으로 용매 분획한 후, 활성이 높은 free phenolic acid부분을 silica gelcolumn chromatography하여 RCF-11 분획하였으며, 이 분획물을 돈지, 팜유 및 대두유에 각각 200 ppm 수준으로 첨가 하였을 때 AI (antioxidant index: 추출물을 넣은 유지의 유도기간을 기질유지의 유도기간으로 나눈 값)는 각각 5.57, 1.53 및 1.54를 나타내었다. 이것은 같은 농도에서의 ${\delta}-tocopherol$, BHT, BHA 보다 동물성유지인 돈지에서는 4배, 식물성유인 팜유와 콩기름에서는 1.5배 정도 효과가 우수하였다. RCF-11에 항산화작용이 알려진 ascorbic acid, citric acid, ${\delta}-tocopherol$ 200 ppm을 첨가한 후 분획물의 농도를 달리해가며 동물성유지와 식물성유지에 적용해보았다. 식물성유에서는 ascorbic acid를 첨가했을 때 단독으로 사용했을 때보다 AI가 1.5배 정도 상승효과가 있었으며, 동물성유에서는 ${\delta}-tocopherol$를 첨가했을 때 단독으로 사용했을 때보다 AI가 2배 정도 상승하였다. Rancimat을 통해 항산화 활성이 확인 된 옻나무 75% ethanol 추출물의 free phenolic acid 성분은 TLC, UV spectra를 통해 본 결과 flavanone이나 flavone계 화합물로 추정되며, 앞으로 그 원인물질에 대한 구조동정이 요구된다.

• 한국식품위생안전성학회지
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• 제10권3호
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• pp.181-187
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• 1995

Water extract, and methanol extract, its chloroform and hexane fractions, and estragole from Agastache rugosa O. Kuntze were tested to find the inhibition effect on the growth of several microorganisms. The organisms used were Escherichia coli ATCC 1129, Staphylococcus aureus IAM 1011, Vibrio parahaemolyticus WP, Bacillus subtilis ATCC6633, Aspergillus oryzae KFCC 890, Aspergillus niger KCCM 11240. Water and methanol extracts at the concentration of 0.5%, and chloroform and hexane fraction at the concentration of 0.05% inhibited the growth of microorganisms from 1/5 to 2/3 of the control group. Estragole identified from the hexane faction as a major component, its authentic compound completely inhibited the growth of Vibrio parahaemolyticus completely at the concentration of 0.03%, and the other bacteria were at 0.05%.

• 생약학회지
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• 제25권1호
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• pp.47-50
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• 1994

For the biological survey, effects of Ailanthi Cortex Radicis, the root bark of Ailanthus altissima(Simaroubaceae) on epoxide hydrolyzing enzymes were checked. The methanolic extract and its chloroform fraction were shown to activate the liver metabolizing enzyme system including epoxide hydrolase system which was monitored by activities of transaminase, lactate dehydrogenase, alkaline phosphatase and epoxide hydrolase system in bromobenzene treated rats. But they showed no effect on glutathione S-transferase activity.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.131-131
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• 1998

In the course of screening for new natural compounds having antioxidant activity from microbials, mushrooms as well as plants. We have isolated novel sesquiterpenoid and officinalic acid from methanolic extracts of Stereum ostrea. The dried fruit body( 450 g) of Stereum ostrea was extracted with methanol, then methanolic extract was partitioned between water and hexane, chloroform, and ethyl acetate, subsequently. The chloroform extract was subjected on silica gel, Sephadex LH-20 and RP-18 column chromatography, successively. And two compounds were isolated and identified as new triterpenoids and officinalic acid using $^1$H - NMR, $\^$13/C - NMR as well as Mass spectra.

• 생명과학회지
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• 제9권3호
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• pp.248-252
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• 1999

Effects of the ultrasonic treatment on plant represents antifeeding activity and chitin synthesis inhibition activity against diamondback moth (Plutella xylostella L.) by a leaf-dipping method at a concentration of 5,000ppm. Methanol extracts of leaves of the ultrasonic treatment on plant was sequentially partitioned with n-hexane, chloroform, ethlyacetate, butanol and water fractions, and bioassayed. Antifeeding activities were observed in n-hexane, chloroform and ethlyacetate fractions. N-hexane fraction has 94.6% of strongest antifeeding activity against P. xylostella. From the n-hexane sequentially partitioned showed H6 and H8 fractions of antifeeding activities against P. xylostella. And chitin synthesis inhibition activity against P. xylostella was obtained the methaol, n-hexane and water fractions.

• 생약학회지
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• 제31권1호
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• pp.72-76
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• 2000

This study was carried out to evaluate cytotoxic effects of Ajuga multiflora Bunge extracts on murine leukemia tumor $(P388D_1)$ cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The comparison of $IC_{50}$ values of Ajuga multiflora Bunge extracts in L1210 and $P388D_1$ cell lines showed that their susceptibility to these extracts decreased in the following order: Adriamycin>methanol extract>chloroform extract>ethyl acetate extract>hexane extract>water extract by the MTT assay. In order to develop an antimicrobial agent, dried Ajuga multiflora Bunge was extracted with several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentration (MIC) of the extract against microorganisms were also examined. Antimicrobial activities of amocla and ketoconazole as references were compared to those of extracts of $H_2O$, n-hexane, chloroform, ethyl acetate and methanol. The antimicrobial activity of all extracts from the sample had growth inhibition activity against gram-negative bacteria, gram-positive bacteria and fungi $(MIC>200\;{\mu}g/ml)$. These results suggest that the methanol soluble extract of Ajuga multiflora Bunge may be a valuable choice for the studies on the treatment of murine leukemia tumor cell lines.

• 한국미생물·생명공학회지
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• 제24권1호
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• pp.105-110
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• 1996

Mugwort has been known as a traditional substitutive foodstuff and as showing a physiologically beneficial function to a human being. Therefore, effect of mugwort extract in terms of mutagenicity and desmutagenicity was investigated to berify its function. Ethanol extract from mugwort did not exhibit any mutagenicity. On the contrary, inhibitory effects of the ethanol extract were observed on mutagenicity induced by aflatoxin $B_{1}(AFB_1)$, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole(Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b]indole(Trp-P-2) and 2-nitroflourene(2NF) using Salmonella typhimurium reversion assay. On direct-acting mutagen(2NF, 3${\mu}$g/plate), ethanol extract showed a slight inhibitory effect of 19.7~22.9%, however on indirect-acting mutagen such as AFB1(2${\mu}$g/plate), Trp-P-1(1${\mu}$g/plate) and Trp-P-2(1${\mu}$g/plate), we observed higher inhibitory effect of 47.9~61.2%, 64.1~70.7%, 67.4~78.7%, respectively. Step-wise fractionation of the ethanol extract was done by using hexane, chloroform, ethyl acetate and water to obtain effective fraction. Among them, hexane, chloroform, and ethyl acetate fractions showed high inhibition of 63.0~80.0%, 77.5~82.1%, and 68.5~83.1%, respectively on the mutagenicity of $AFB_1$ in Sal. typhimurium TA98. Consequently, these results indicated that mugwort extract contains some compound(s) which may show desmutagenicity.