• Journal of Korean Society of Water and Wastewater
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• v.25 no.3
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• pp.307-312
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• 2011

The potential of algal-bacterial culture was investigated for advanced treatment of animal wastewater. Fed-batch experiments were carried out to examine treatability of nitrogen and phosphorus in different microbial consortium: Chlorella vulgaris, activated sludge, three microalgae strains (Scenedesmus, Microcystis, Chlorella) and Bacillus consortium, and three microalgae strains and sludge consortium. Single culture of C. vugaris showed the better efficiency for nitrogen removal but was not good at organic matter and phosphorus removal compared with activated sludge. Three microalgae and Bacillus consortium was best culture among the culture and consortium for pollutants removal tested in this experiment. Effect of $CO_2$ addition was studied by using three microalgae and Bacillus consortium. $CO_2$ addition enhanced T-P removal efficiency up to 60%. However, removal efficiencies of T-N and ammonia nitrogen reduced on the contrary.

• Korean Journal of Poultry Science
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• v.40 no.3
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• pp.271-276
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• 2013

A study was conducted to investigate the effect of dietary supplementation of feedstuff of Chlorella (Chlorella vulgaris) to replace of antibiotic in the diets of broiler chickens. A total of 720 1-d-old straight run broiler chicks (Ross ${\times}$ Ross) was randomly assigned into six treatments with four replicate pens (30 birds/replicate pen) for 5-wk. A corn-soy bean meal basal diet was formulated, the treatment groups were negative group (NC, antibiotic-free diet) and 0.1% virginiamycin in as antibiotic growth promoters (PC), 1.0% fresh liquid Chlorella (T1), 1.0% dried Chlorella powder (T2), 1.0% commercial Chlorella product and 1.0% (T3) and commercial Chlorella product 0.5% (T4) were added to the basal diet to form six dietary treatments. No significant differences were found among the treatments for feed intake and feed conversion of broiler chickens during the whole experimental period, but the BW gain was significantly higher (P<0.05) in commercial Chlorella product supplemental groups than the control group (NC and PC groups). Dietary supplementation of Chlorella significantly (P<0.05) increased the plasma IgA, IgM and IgG concentration of chicks compared to NC and PC groups. Supplemental AGPs and commercial chlorella product did not affect the E. coli and Salmonella concentration in the intestinal microflora of broiler chicks; however, the population of Lactobacillus was significantly increased (P<0.05) when birds were fed commercial Chlorella product groups. It is concluded that commercial Chlorella product supplementation could be used as an alternative of antibiotics to promote growth and immune response by increasing the production of lactic acid bacteria in the intestinal microflora of broiler chickens.

• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.3
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• pp.236-245
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• 2016

Because microalgae represent high growth rate than terrestrial plants, and it can accumulate significant lipid and carbohydrate content, and other bioactive compounds such as carotenoid and polyphenol in their body, it has been considered as one of the promising resources in bio-energy, and other industries. Although many studies has been performed about the microalgae-derived biochemical accumulation under various abiotic conditions such as different temperatures, salinities and light intensities, the studies about simultaneous effect of those parameters has rarely been performed. Therefore, this study focused on evaluation of simultaneous effect of different salinity (10, 30, 50 psu) and temperatures (20, 25, $30^{\circ}C$) on the changes of biomass, lipid, starch and photosynthetic pigment accumulation. As results, the highest growth rate was achieved at $30^{\circ}C$ and 30 psu in the both algal cultures, and the photosynthetic pigment, chlorophyll a and total carotenoid content, were increased in a temperature-dependent manner. The accumulation of lipid and starch contents exhibited different aspects under different combinations of temperature and salinity. From the results, it is suggested that the changes of microalgal lipid and starch accumulation under different salinities may be affected by the different temperatures.

• The Korean Journal of Pesticide Science
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• v.12 no.1
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• pp.82-87
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• 2008

Algae are vital in the primary production of the aquatic ecosystem, having been considered as good indicators of the bioactivity of pesticides. Algae have short life cycle, respond quickly to environmental change and their diversity and density can indicate the quality of their habitat. The purpose of the study was to determine the growth inhibition effects of butachlor (Tech. 93.4%) and $K_2Cr_2O_7$ (Tech. 99.5%) in Selenastrum capriconutum, Scenedesmus subspicatus, Chlorella vulgaris and Nitzschia palea during and exposure period of 72 hours. The toxicological responses of S. capriconutum, S. subspicatus, C. vulgaris and N. Palea to butachlor, expressed in individual $ErC_{50}$ values were 0.0022, 0.019, 8.67 and $4.94\;mg\;L^{-1}$, respectively. NOEC values were 0.0008, 0.0016, 5.34 and $2.92\;mg\;L^{-1}$, respectively. S. capriconutum was more sensitive than the other algae species. The toxicological responses of S. capriconutum, S. subspicatus, C. vulgaris and N. palea to $K_2Cr_2O_7$ expressed as $ErC_{50}$ values were 0.91, 0.78, 0.85 and $0.57\;mg\;L^{-1}$, respectively. NOEC values were 0.2, 0.2, 0.2 and $0.18\;mg\;L^{-1}$, respectively. Growth inhibition of S. capriconutum, S. subspicatus, C. vulgaris and N. palea from PEC of butachlor were 100, 75, 0 and 0%, respectively.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.900-908
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• 2016

The effects of algal inoculation on chemical oxygen demand (COD) and total nitrogen (TN) removal, and indigenous bacterial dynamics were investigated in municipal wastewater. Experiments were conducted with municipal wastewater inoculated with either Chlorella vulgaris AG10032, Selenastrum gracile UTEX 325, or Scenedesmus quadricauda AG 10308. C. vulgaris and S. gracile as fast growing algae in municipal wastewater, performed high COD and TN removal in contrast to Sc. quadricauda. The indigenous bacterial dynamics revealed by 16S rRNA gene amplification showed different bacterial shifts in response to different algal inoculations. The dominant bacterial genera of either algal case were characterized as heterotrophic nitrifying bacteria. Our results suggest that selection of indigenous bacteria that symbiotically interact with algal species is important for better performance of wastewater treatment.

• Korean Journal of Environmental Biology
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• v.20 no.3
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• pp.256-262
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• 2002

This research investigated experimentally on the population growth in the aquatic microcosm with the wastewater of plating factory. The purpose of this study was to evaluate the effect of culture conditions of the characteristic growth pattern of the examined species. Population of the system is consists of three organisms; Chlorella vulgaris as a producer, Cyclidium glaucoma as a consumer and Pseudomonas putida as a decomposer. The different growth patterns of each population are followed by surfactant type; Especially C. glaucoma was sensitive, Ch. uvlgaris was maintained population size stably even at high level of surfactant and p. putida was not significantly affected. After treatment of waste water from plating factory, it began to be affected at 1.0% solution treatment to Ch. vulgaris which the cell number was decreased prominently after 2 days, and C. glaucoma was disappeared at 2.5% solution treatment. P. putida was showed increasing pattern according to treatment concentration, at 2.5% solution and population size grew double. The result from current microcosm study indicates that this model system can be applied to environmental assessment method for various pollutants.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.6
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• pp.920-926
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• 2010

Marine alga, Chlorella vulgaris, was extracted by chloroform-methanol (2:1, v/v) solvents for lipid extraction at $35^{\circ}C$ for five hours (HCM-35) and its process was compared with conventional lipid extraction condition such as chloroform-methanol (2:1, v/v) at $65^{\circ}C$ for one hour (CM-65). This low temperature extraction process showed that 80% of total lipid was extracted and its residues contained relatively unchanged amounts of intact proteins and other minerals as well as amino acid profiles. Interestingly enough, the weight fraction of carbohydrate in the residues slightly increased due to less denaturation at low process temperature. The biological activities of the residues such as cytotoxicity and immune cell growth activation were not much changed after being extracted. The sensory evaluation were found to be very favorable for being used as a food additive and/or food supplement. This result could also help to maintain the economic feasibility of utilizing marine resources in food and other relevant industries.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.2
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• pp.315-322
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• 2016

This study determined the optimum microalgae species and concentration for growth of small rotifer Proales similis, in community cultures by feeding it several different foods. Four single trials (Tetraselmis suecica, TET; Isochrysis galbana, ISO; Nannochloropsis oculata, NAN; freshwater Chlorella vulgaris, CHL) were conducted at 5 psu and $25^{\circ}C$. The maximum density and specific growth rate (SRG) were highest for rotifer fed CHL reaching 1,075 individuals (inds.)/mL and 0.83 respectively. By contrast, other foods of ISO, NAN and TET resulted in poor growth rates and maximum density. In the freshwater C. vulgaris experiments examining a range of 0.05 to $8.8mg/10^4rotifers$, the growth of rotifer tended to decrease with the amount of supplement. The maximum density and SGR of females was highest at 910 inds./mL and 0.55 respectively, at $0.05mg/10^4rotifers$. These results suggest that the best microalgae species for the culture of P. similis is freshwater C. vulgaris and the optimum concentration is $0.05mg/10^4rotifers$.

• Korean Journal of Ecology and Environment
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• v.42 no.1
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• pp.67-74
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• 2009

To study the influence of the rotifer Brachionus calyciflorus on harmful algal bloom suppression, we focused on assessing the rotifer's abilities using several prey species : Microcystis aeruginosa, Synechocystis sp., Chlorella vulgaris and Coelastrum sp. of the warm-weather species and the cold-weather centric diatom Stephanodiscus hantzchii. Grazing effects and growth rates of rotifers B. calyciflorus were 94.5% and $1.29d^{-1}$, respectively, for Synechocystis sp., 87.4% and $0.60d^{-1}$, respectively, for M. aeruginosa, 95.2% and $0.65d^{-1}$, respectively, for C. $vulgaris^{TM}$, 78.6% and $0.45d^{-1}$, respectively, for C. vulgaris UTEX., 86.5% and $0.99d^{-1}$, respectively, for Coelastrum sp., and 82.6% and $0.40d^{-1}$, respectively, for S. hantzchii. Of these, although the growth of Synechocystis and Coelastrum was effectively suppressed by rotifer grazing, efficient suppression effects on Stephanodiscus blooms were unexpected. The present study revealed that reproduction of B. calyciflorus was greatly influenced by its food types in the initial stages and the efficiencies of bio-agents as sole food sources vary depending on the target algae and the agent.

• Journal of Korean Society on Water Environment
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• v.39 no.2
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• pp.175-180
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• 2023

The aim of this study was to compare the fouling characteristics of Extracellular polymeric substances (EPS) secreted by Chlorella vulgaris with the case of Bacterial-MBR (BMBR), Microalgal-MBR (MMBR) for advanced wastewater treatment using the Laboratory scale, in order to suggest a method to minimize fouling in MMBR by identifying the effects of amounts and compositions of EPS secreted by C. vulgaris and bacteria in the activated sludge on fouling. Contrary to expectations, fouling occurred relatively severely in the MMBR from the beginning of the operation than in the BMBR. Reasons for such a fouling pattern were considered to be the effect of C-EPS, which accumulates on the membrane surface of MMBR 30 times more than that on the membrane surface of activated sludge (BMBR). In this respect, according to the results of this experiment and a comparative review of several previous studies, it was confirmed that unlike activated sludge, in which the ratio of P-EPS was relatively higher than that of C-EPS, in case of C. vulgaris, the ratio of C-EPS to P-EPS was relatively higher than that in case of activated sludge. This was presumed to be the main cause of the significant fouling phenomenon in MMBR. However, an increase in TMP with increasing C-EPS concentration was not observed.