Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effect of Dietary Chlorella Supplementation on Growth Performance, Immune Response, and Intestinal Micro Flora Concentration of Broiler Chickens

육계 사료 내 클로렐라의 첨가·급여가 생산성, 장내미생물 및 면역력에 미치는 영향

  • Kang, Hwan Ku (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Choi, Hee Chul (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Kim, Dong Woon (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Hwangbo, Jong (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Na, Jae Cheon (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Bang, Han Tae (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Kim, Dong Wook (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Kim, Min Ji (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Mushtaq, M.M.H. (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Parvin, Rana (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Kim, Ji Hyuk (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science)
  • 강환구 (농촌진흥청 국립축산과학원 가금과) ;
  • 최희철 (농촌진흥청 국립축산과학원 가금과) ;
  • 김동운 (농촌진흥청 국립축산과학원 가금과) ;
  • 황보종 (농촌진흥청 국립축산과학원 가금과) ;
  • 나재천 (농촌진흥청 국립축산과학원 가금과) ;
  • 방한태 (농촌진흥청 국립축산과학원 가금과) ;
  • 김동욱 (농촌진흥청 국립축산과학원 가금과) ;
  • 김민지 (농촌진흥청 국립축산과학원 가금과) ;
  • 하룬 무스타크 (농촌진흥청 국립축산과학원 가금과) ;
  • 라나 파빈 (농촌진흥청 국립축산과학원 가금과) ;
  • 김지혁 (농촌진흥청 국립축산과학원 가금과)
  • Received : 2013.09.03
  • Accepted : 2013.09.24
  • Published : 2013.09.30
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Abstract

A study was conducted to investigate the effect of dietary supplementation of feedstuff of Chlorella (Chlorella vulgaris) to replace of antibiotic in the diets of broiler chickens. A total of 720 1-d-old straight run broiler chicks (Ross ${\times}$ Ross) was randomly assigned into six treatments with four replicate pens (30 birds/replicate pen) for 5-wk. A corn-soy bean meal basal diet was formulated, the treatment groups were negative group (NC, antibiotic-free diet) and 0.1% virginiamycin in as antibiotic growth promoters (PC), 1.0% fresh liquid Chlorella (T1), 1.0% dried Chlorella powder (T2), 1.0% commercial Chlorella product and 1.0% (T3) and commercial Chlorella product 0.5% (T4) were added to the basal diet to form six dietary treatments. No significant differences were found among the treatments for feed intake and feed conversion of broiler chickens during the whole experimental period, but the BW gain was significantly higher (P<0.05) in commercial Chlorella product supplemental groups than the control group (NC and PC groups). Dietary supplementation of Chlorella significantly (P<0.05) increased the plasma IgA, IgM and IgG concentration of chicks compared to NC and PC groups. Supplemental AGPs and commercial chlorella product did not affect the E. coli and Salmonella concentration in the intestinal microflora of broiler chicks; however, the population of Lactobacillus was significantly increased (P<0.05) when birds were fed commercial Chlorella product groups. It is concluded that commercial Chlorella product supplementation could be used as an alternative of antibiotics to promote growth and immune response by increasing the production of lactic acid bacteria in the intestinal microflora of broiler chickens.

본 실험은 액상 클로렐라, 분말 클로렐라 및 클로렐라 사료 첨가제 0.5%, 1.0%를 육계 사료 내 첨가 급여하였을 때 생산성, 혈액 성상 및 장내 미생물에 미치는 영향을 알아보고자 시험을 실시하였다. 육계(Ross) 초생추 총 720수를 공시하여 6처리 4반복, 반복 당 30수씩 공시하여 5주간 시험을 실시하였다. 처리구로는 항생제를 첨가하지 않은 처리구(NC, negative control), 항생제 처리구(PC, positive control), 액상 클로렐라 1.0% 첨가구 (T1), 분말 클로렐라 1.0% 첨가구 (T2), 클로렐라 사료 첨가제 1.0% 첨가구 (T3) 그리고 클로렐라 0.5%(T4)로 나누어 실험하였다. 종료 시 체중은 무항생제 처리구(NC, negative control)에서 1,635 g 항생제 처리구(positive control) 1,655 g, 액상 클로렐라 첨가구 1,654 g, 분말 클로렐라 첨가구 1,653 g 그리고 클로렐라 사료 첨가제 1.0% 및 0.5%에서 각각 1,672 g, 1,670 g으로 나타나, 클로렐라 사료 첨가제 처리구에서 무항생제 처리구와 비교 시 평균 2% 이상 유의적으로 높게 나타났다(P<0.05). 장내 미생물 조사에서는 클로렐라 사료 첨가제 첨가구에서 유산균수는 각각 7.65 CFU/g, 7.49 CFU/g으로 나타나 유의적으로 증가하였으나 E. coli 및 Samonella 함량은 차이가 없었다. 혈액 내 IgG, IgA 그리고 IgM 역시 클로렐라 함유 사료 첨가제에서 유의적으로 개선되었다(P<0.05). 결과적으로 육계사료 내 클로렐라 함유 사료첨가제의 첨가 급여는 장내 미생물 중 유산균 함량과 혈액 면역 물질을 유의적으로 증가시킴으로써 육계 생산성을 개선시키는 것으로 나타났다. 하지만 국내 외적으로 가축 사료 내 클로렐라를 활용한 연구가 많지 않기 때문에, 앞으로 보다 진보적인 연구가 필요할 것으로 사료된다.

Keywords

References

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