• 한국식품과학회지
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• 제30권4호
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• pp.855-861
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• 1998

${\kappa}$-카라기난과 키토산을 혼합하여 제조한 혼합 필름의 인장강도, 신장률, 수증기 투과도를 측정하였다. ${\kappa}$-카라기난과 키토산 고분자의 분자량은 light-scattering instrument를 이용하여 측정하였으며, ${\kappa}$-카라기난과 키토산의 분자량은 $5.1{\times}10^5,{\;}1.71{\times}10^5$로 각기 측정되었다. ${\kappa}$-카라기난과 키토산 필름의 인장강도는 각각 30.2 MPa, 3.2 MPa로 나타났으며, 혼합 필름은 21.0 MPa을 나타내었다. 혼합 필름의 인장강도는 환원제의 첨가$(1{\sim}3%)$에 영향을 받지 않는 것으로 나타났으며, 신장률은 각각의 단일 고분자 필름에 비해서 낮은것으로 나타났다. 혼합 필름의 수증기 투과도는 키토산 free 필름과 ${\kappa}$-카라기난 free 필름의 수증기투과도에 비하여 $1.4{\sim}2.5$배 낮은 것으로 나타났다. ${\kappa}$-카라기난과 키토산 혼합 필름은 ${\kappa}$-카라기난의 황산기$(SO_3^-)$와 키토산의 아미노기$(NH_3^+)$가 정전기적 결합에 의하여 형성되는 것으로 예상된다.

• 한국환경보건학회지
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• 제24권3호
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• pp.48-53
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• 1998

Chitin is known as biodegradable natural polymer. But, in spite of various application of chitin from waste marine sources, commercial use of chitin has been limited due to highly resistance to chemicals and the absense of proper solvents. We made various viscosity of chitosan from chitin by change of Mima's method through the deacetylation which is various condition of NaOH concentration, reaction time and temperature. Also, Polyvinyl alcohol/chitosan blend films were prepared by different solution blends containing the ratio of 5, 10, 15 and 20% chitosan and low, medium, high molecular weight of chitosan to find a more useful biodegradable polymer. Thermal and mechanical properties of PVA/chitosan blend films such as DSC, impact strength, tensile strength and morphological changes by SEM were determined. The 10-15% PVA/chitosan(low, medium) blend films were similar to PVA. Also, PVA/chitosan blend films at the laboratory soil test(Pot Test) were completely degraded in month with four kinds of soils by microorganisms.

• 한국식품위생안전성학회지
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• 제14권1호
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• pp.90-96
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• 1999

Biodegradable films were prepared by solution blending method in the weight ratio of Chitosan and Algin for the purpose of useful bioimplants. The possibility of bioimplants, which prepared from natural polymers as a skin substitute and food wrapping materials were evaluated by measuring the biodegradability. these biodegradable films were inserted in the back of rats and their biodegradability was investigated by hematological change as a function of time. Rats study showed that low-Chitosan induced increments of monocyte and basophil after 48 hours of implantation. And medium-Chitosan showed increase of lymphocyte and decreased neutrophil counts after 48 hours of implantation. Low, medium Chitosan showed high hemoglobin contents, medium and high Chitosan showed high hematocrit value after 48 hours of implantation. As a result, medium, high-Chitosan induced potential incompatibility in the tissue after 48 hours, but there was little effects to the skin inflammation. The values of biodegradable films, which prepared from natural polymers measured in this study were some satisfiable results at short period with those of ideal skin bioimplants and artificial skin.

• 약학회지
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• 제33권6호
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• pp.324-332
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• 1989

Drug release from sulfanilamide granules coated with a polyelectrlyte complex of sodium tripolyphosphate and chitosan was studied. The coating film thickness increased with increasing concentration of chitosan in the coating solution and the drug release rates of the coated granules were significantly reduced comparing with those of the uncoated granules. $T_{50%}$ of the uncoated granules was 6 minutes, but those of the granules coated with chitosan-sodium tripolyphosphate from 0.5, 0.7, and 0.9% (w/v) chitosan-HCl solution were 27, 135, and 180 minutes, respectively in distilled water. In dissolution medium at pH 6.8, $T_{50%}$ of the uncoated granules was 4 minutes, but those of the granules coated with chitosan-sodium tripolyphosphate from 0.5, 0.7, and 0.9(w/v)% chitosan-HCl solution, were 32, 135, and 160 minutes, respectively.

• 한국식품영양과학회지
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• 제33권7호
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• pp.1224-1229
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• 2004

PHB/chitosan 필름의 수분 투과도는 PHB 필름께서 280.25 g/m$^2$ㆍ24 hr로 가장 높았고 chitosan의 함량이 증파할수록 수분 투과도는 낮아지는 경향을 보였다. PEG가 첨가된 PHB/chitosan 필름에서도 PHB-P 필름의 수분 투과도가 294.5 g/m$^2$ㆍ24 hr로 가장 높았고, chitosan의 함량이 증가할수록 수분 투과도는 낮아졌다. 산소 투과도의 경우 PHB 필름이 0.026${\times}$$10^{-10}$$cm^3$(STP) cm/$cm^2$ㆍsecㆍcmHg로 가장 높았으며 chitosan의 첨가 비율이 증가할수록 산소 투과도는 낮아졌다. 또한 가소제인 PEG가 첨가되지 않은 필름이 PEG가 첨가된 필름보다 산소 투과도가 더 낮았다. 유지 투과도에서는 PHB와 PHB-P를 제외한 필름들이 24시간동안 유지론 통과시키지 않아 유지 차단성이 우수한 것으로 나타났다. 필름의 생분해도 측정시 필름을 첨가하지 않은 대조군에 비해 필름이 첨가된 실험군의 산소 소모량이 높아 필름이 생분해되고 있음을 확인할 수 있었으며 필름의 PHB의 비율이 높을수록 분해도가 높은 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제26권5호
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• pp.953-958
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• 2016

Chitosan-based film-forming gel is regarded as a promising vehicle for topical delivery of antimicrobial agents to skin wounds, since it protects from microbial infection and the cationic polymer itself possesses antibacterial activity. In this study, possible synergistic interaction against common skin pathogens between the cationic polymer and tyrothricin (TRC), a cyclic polypeptide antibiotic, was investigated, by determining the concentration to inhibit 90% of bacterial isolates (MIC). The addition of the polysaccharide to TRC dramatically reduced the MIC values of TRC by 1/33 and 1/4 against both methicillin-resistant and methicillin-susceptible Staphylococcus aureus, respectively. The synergism of TRC and chitosan combination against both strains was demonstrated by the checkerboard method, with a fractional inhibitory concentration index below 0.5. Moreover, co-treatment of TRC and chitosan exhibited antibacterial activity against Pseudomonas aeruginosa, due to the antibacterial activity of chitosan, whereas TRC itself did not inhibit the gram-negative bacterial growth. These findings suggested that the use of chitosan-based film for topical delivery of TRC could be an alternative to improve TRC antimicrobial activity against strains that are abundant in skin wounds.

• 폴리머
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• 제33권5호
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• pp.509-513
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• 2009

키토산/피브로인 블렌드의 구조와 물성에 미치는 브롬화 리튬의 효과를 고찰하기 위하여, 브롬화 리튬을 함유한 용액으로 성형한 키토산/피브로인 블렌드 필름과 성형 시 브롬화 리튬을 제거한 블렌드 필름의 구조적 특성을 조사하였다. 키토산/피브로인 블렌드는 용해된 브롬/리튬이온과 더불어 복합체를 형성하였고, 0.6 mol/L의 LiBr 농도에서 X-선 회절에 의한 복합체의 결정성을 보였으며, 이온 농도가 증가할수록 결정성은 감소되었다. 복합체 용액으로부터 고화 시중화 및 삼투작용으로 브롬화 리튬을 제거한 블렌드 필름의 결정은 키토산의 수화형 결정상을 형성하였으며, 브롬화 리튬을 처리하지 않은 것에 비하여 결정성이 크게 증가하였다. 복합체에 의한 블렌드 필름은 자중의 수십 배의 물을 흡수하여 수화겔을 형성하였다.

• 한국축산식품학회지
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• 제36권3호
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• pp.377-388
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• 2016

Objective of the present study is to investigate the effect of coated edible films with chitosan solutions enriched with essential oil (EO) on the chemical, microbial and sensory properties of Kashar cheese during ripening time. Generally, no differences were found in total aerobic mesophilic bacteria, streptococci and lactoccocci counts among cheeses but these microorganism counts increased during 60 and 90 d storage especially in C1 (uncoated sample) as compared with coated samples. Antimicrobial effectiveness of the films against moulds was measured on 30, 60, and 90 d of storage. In addition of fish EO into chitosan edible films samples were showed to affect significantly decreased the moulds (p<0.05) as 1.15 Log CFU/g in C4 (with fish oil (1% w/v) fortified chitosan film) on the 90^th d, while in C1 as 3.89 Log CFU/g on the 90^th d of ripening. Compared to other cheese samples, C2 (coated with chitosan film) and C4 coated cheese samples revealed higher levels of water-soluble nitrogen and ripening index at the end of storage. C2 coated cheese samples were preferred more by the panellists while C4 coated cheese samples received the lowest scores.

• 한국수산과학회지
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• 제25권1호
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• pp.51-57
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• 1992

폐기되고 있는 꽂게껍질로 만든 키토산필름의 포장에 의한 반염건전갱이제품의 제조 및 저장중품질유지에 미치는 영향에 대해 실험하였다. 대조제품과 키토산필름포장제품의 수분함량은 각각 $60.6\%,\;65.0\%$로서 키토산필름포장제품의 수분함량이 $4.4\%$ 높았다. 저장중 pH, 휘발성염기질소 및 아미노질소의 증가폭은 키토산필름포장제품이 적었다. 생균수는 저장초기에 약간 감소하다가 그 이후로 계속 증가하여 대조제품은 14일째에 $8.0\times10^5/g$이었으나 키토산필름포장제품은 저장 20일째에도 $7.0\times10^5/g$이었다. TMAO는 저장중 감소하고 TMA는 증가하는 경향이었고 그 증감의 폭은 키토산필름포장제품이 적었다. TBA값과 과산화물값은 저장중 증가하다가 저장 14일 이후로 감소하였고 증가폭은 키토산필름포장제품이 적었다. 관능검사결과 저장 14일째에 대조제품은 키토산필름포장제품에 비해 현저히 품질이 떨어졌고 키토산필름포장제품은 저장 14일째에도 식용가능하였다. 이화학적 및 관능적평가로 미루어 보아 키토산필름포장 제품은 대조제품에 비해 품질유지면에서 우수하다는 결론을 얻었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.46-46
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• 2019

The experiments were performed in the Jinan (elevation: 300 meters above sea level), Jeollabuk-do. Seedlings (n = 63 per $3.3m^2$) of ginseng cultivar (Cheonpung, Yeonpung) were planted on April 10, 2015. Shading material of plastic film house was blue-white film. Before the Planting seedling, silicate (3 kg/10 a) or chitosan (40 kg/10 a) was fertilized and foliar sprayed on the leaves 1000 times dilution solution once a month from May to September every year. The growth results of 5-year old ginseng surveyed in 2018 are as follows. The average air temperature in the plastic film house was the highest at $26.6^{\circ}C$ and $26.5^{\circ}C$ in July and August, respectively, and the highest temperature was $40.5^{\circ}C$ in July. The maximum daily temperature of $35^{\circ}C$ or more was 30 days, with the average soil temperature being $24.9^{\circ}C$ in August. The chemical properties of the test soil are as follows. pH was 6.4~6.9 level and EC was 0.35~0.46 dS/m. The organic matter content was 33.5~41.4 g/kg, and available-P content was 251.9~306.8 mg/kg. Exchangeable cations contents, such as K, Ca and Mg were all the appropriate ranges. The soil microbial density surveyed by the dilution plate method was 10~50 times higher than that of control (Non-treatment) and actinomycete density was 3~6 times higher. Pathogens of the genus Fusarium by Metagenome analysis decreased 91.3% and 68.2% respectively in the foliar sprayed of chitosan and soluble-silicate. The light intensity (PAR) in the blue-white film plastic film house gradually increased until July and then decereased, with the average of light intensity in March-October was $120.3umol/m^2/s$. The growth of aerial parts such as plant height and stem length was better than non-sprayed group in silicate or chitosan treatments and Yeonpung cultivar was superior to the Cheonpung cultivar. The SPAD value was higher in Yeonpung cultivar foliar sprayed with soluble-silicate. The growth of underground parts such as root length and taproot length were better in chitosan and soluble-silicate treatment than control, especially in Yeonpung cultivar foliar sprayed with chitosan was good in taproot length and taproot diameter, and fresh weight of root was 60.1 g. Ginsenoside contents were 24.9 mg/g and 22.4 mg/g, in the Cheonpung cultivar foliar sprayed with soluble-silicate or chitosan respectively, 28% and 15% higher than control (19.5 mg/g). The incidence of disease by Alteraria panax and Botrytis cinerea was 3~9% and 4~9%, respectively. High temperature damage rate was 3~5%.