• Genes and Genomics
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• v.40 no.12
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• pp.1339-1349
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• 2018

Cerebral palsy (CP) is a non-progressive neurological disease, of which susceptibility is linked to genetic and environmental risk factors. More and more studies have shown that CP might be caused by multiple genetic factors, similar to other neurodevelopmental disorders. Due to the high genetic heterogeneity of CP, we focused on investigating related molecular pathways. Ten children with CP were collected for whole-exome sequencing by next-generation sequencing (NGS) technology. Customized processes were used to identify potential pathogenic pathways and variants. Three pathways (axon guidance, transmission across chemical synapses, protein-protein interactions at synapses) with twenty-three genes were identified to be highly correlated with CP. This study showed that the three pathways associated with CP might be the molecular mechanism of pathogenesis. These findings could provide useful clues for developing pathway-based pharmacotherapies. Further studies are required to confirm potential roles for these pathways in the pathogenesis of CP.

• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1213-1227
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• 2023

Fetal growth restriction (FGR) is a prevalent obstetric condition. This study aimed to investigate the role of Toll-like receptor 9 (TLR9) in regulating the inflammatory response and gut microbiota structure in FGR. An FGR animal model was established in rats, and ODN1668 and hydroxychloroquine (HCQ) were administered. Changes in gut microbiota structure were assessed using 16S rRNA sequencing, and fecal microbiota transplantation (FMT) was conducted. HTR-8/Svneo cells were treated with ODN1668 and HCQ to evaluate cell growth. Histopathological analysis was performed, and relative factor levels were measured. The results showed that FGR rats exhibited elevated levels of TLR9 and myeloid differentiating primary response gene 88 (MyD88). In vitro experiments demonstrated that TLR9 inhibited trophoblast cell proliferation and invasion. TLR9 upregulated lipopolysaccharide (LPS), LPS-binding protein (LBP), interleukin (IL)-1β and tumor necrosis factor (TNF)-α while downregulating IL-10. TLR9 activated the TARF3-TBK1-IRF3 signaling pathway. In vivo experiments showed HCQ reduced inflammation in FGR rats, and the relative cytokine expression followed a similar trend to that observed in vitro. TLR9 stimulated neutrophil activation. HCQ in FGR rats resulted in changes in the abundance of Eubacterium_coprostanoligenes_group at the family level and the abundance of Eubacterium_coprostanoligenes_group and Bacteroides at the genus level. TLR9 and associated inflammatory factors were correlated with Bacteroides, Prevotella, Streptococcus, and Prevotellaceae_Ga6A1_group. FMT from FGR rats interfered with the therapeutic effects of HCQ. In conclusion, our findings suggest that TLR9 regulates the inflammatory response and gut microbiota structure in FGR, providing new insights into the pathogenesis of FGR and suggesting potential therapeutic interventions.

• Child Health Nursing Research
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• v.30 no.3
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• pp.187-198
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• 2024

Purpose: This study aimed to implement a child abuse prevention program and evaluate its effectiveness based on the Nursing Model of Resilience and Coping Skills Training Model for unmarried mothers during pregnancy and puerperium. Methods: This study had a prospective single-case, AB design with four repeated self-questionnaire measures and three observational measures. Seven unmarried mothers were provided with 10 sessions child abuse prevention program through individual visits from 32 to 34 weeks of pregnancy to six weeks after childbirth. The questionnaire was composed related to resilience, maternal stress, maternal attitude, parent-child interaction, child abuse potential. The observation was measured by video recording (total 16 times) the interaction of parent-child during feeding and analyzing it by three experts. Data were analyzed by Wilcoxon signed-rank test and Friedman's test. Results: Maternal attitude and parent-child interaction were statistically significantly improved after intervention compared to before intervention. However, maternal stress decreased after intervention compared to before intervention, but it was not statistically significant. Additionally, resilience and child abuse potential were not statistically significant. This program is partially effective in preventing child abuse by promoting parenting attitudes and parent-child interactions. Conclusion: This study focused on individual resilience and applied systematic intervention as coping skills training to prevent child abuse. This study is meaningful in that interventions were conducted through individual visits to unmarried mothers at high risk of child abuse, and the program was applied, including pregnancy and postpartum periods, to prevent child abuse early.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.6
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• pp.3648-3657
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• 2014

This study aims to enhance child care teachers' ability to offer health care by developing an educational program for children's health care and safety. This study was carried out using a one-group pretest-posttest design based on a survey of 400 child care teachers in 3 cities. The educational program for child care teachers consisted of three parts: child health observations, disease management for child and emergency care for children. The educational program was conducted 3 times for 3 hours, totaling 9 hours. The educational program showed better grades of child care teachers in child health observation, emergency care for children, and disease management for children. The finding indicates that more child care teachers will adopt a health care education program to improve the quality of child care service.

• Child Health Nursing Research
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• v.22 no.4
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• pp.265-278
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• 2016

Purpose: The aim of this study was to conduct a systematic review and to describe the characteristics of child abuse screening instruments. Methods: Articles regarding the development of a child abuse screening instrument were investigated using the systematic review method. A literature search using the keywords "child and abuse or maltreatment and instrument or screening tool" in English, and "child," "abuse," and "instrument" in Korean, was conducted of material published in PubMed, PsycINFO, CINAHL, SCOPUS, ERIC, and RISS. Database and bibliographic searches, and quality appraisal using the Quality Assessment of Diagnostic Accuracy Studies tool that included systemic reviews, yielded 17 records. Results: Key elementary child abuse screening instruments were developed for physical, psychiatric, affective and sexual and child neglect assessment. The instruments' target populations were children at home and in institutions. The reviewed instruments had the advantage of diagnosing past, concurrent, and indirectly, potential child abuse. Conclusion: The results of this study demonstrate that child abuse screening instruments are available for screening and for assessment of abused children in various circumstances. This review of child abuse screening instruments offers evidence for the acceptable use of optimal psychometric tools for child abuse assessment and provides guidelines for child health nursing practice.

• Korean Journal of Health Education and Promotion
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• v.28 no.1
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• pp.115-130
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• 2011

Objective: This study aimed to introduce Health Impact Assessment using the case of a HIA on 2008 Dream Start Project in Cheongju. Methods: We followed the typical procedure of HIA recommended by the Merseyside Guidelines on HIA. In scoping, the steering committee decided 5 key domains of child health to be assessed: prenatal care, vaccination, nutrition, access to health care, and child abuse and also the methods of collecting the evidence. The HIA appraisal team collected information from various sources including literature, community survey, and focus group interviews. The HIA appraisal team also synthesized the collected information in terms of the nature of health impacts and equity and made recommendations accordingly. Results: Positive impacts were expected in prenatal care, vaccination, and nutrition, while negative impacts were expected in access to health care. The impact of Dream Start on child abuse was uncertain. Several recommendations were made and submitted to the Dream Start team for their consideration. About 2 years later, we found many of them were implemented in 2009 Dream Start project. Conclusion: HIA was found to be applicable and effective to make decision makers in the welfare sector consider health in their work.

• Child Health Nursing Research
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• v.23 no.4
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• pp.479-493
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• 2017

Purpose: This study was done to conduct a meta-analysis of articles describing randomized controlled trials which showed substantiated effects for home-based child abuse prevention programs for all parents. Methods: The articles regarding child abuse prevention programs for parents were investigated using the systematic review method. A literature search was conducted in PubMed, CINAHL, PsycINFO, Cochrane central library, Eric, and RISS on material published. Article searches and quality appraisal through RoB (Risk of Bias) yielded 14 records. Results: The total random effect size was 0.344 (Parents outcome, 0.333; Child outcome, 0.310; Environment, 0.275). The home based intervention for parents was effective for prevention of potential child abuse. Conclusion: This study supports the effectiveness of health care professional directing child abuse prevention programs for all parents whose children are in the early years of life including during the period of pregnancy.

• Research in Community and Public Health Nursing
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• v.15 no.1
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• pp.76-83
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• 2004

Purpose: This study was aimed at investigating the relationship between retention of the maternal child health handbook, awareness of DPT additional immunization and DPT additional immunization, and to provide basic information to enhance the DPT additional immunization rate. Method: The study subjects were 312 women whose children were between four and six years old, and residing in six provinces of Gyungsangnam do. Data was collected with a 10 item questionnaire by interviews from July 1st to 30th, 2003, and analyzed with descriptive statistics and X2 test at a significance level of 0.05, by two tailed test. Results: The awareness of DPT additional immunization was significantly higher in the women who retained the maternal child health handbook, than their counterparts. The rate of DPT additional immunization was significantly higher in the women who were aware of the DPT additional immunization or who retained the maternal child health handbook, than their counterpart. Conclusion: The DPT additional immunization rate was related to retention of the maternal child health handbook and awareness of DPT additional immunization. Therefore it is recommended that measures be developed and taken to increase the retention rate of the maternal child health handbook and awareness of DPT additional immunization, to enhance the rate of DPT additional immunization.

• Korean Parent-Child Health Journal
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• v.13 no.1
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• pp.44-53
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• 2010

Purpose: This study was conducted to identify the educational needs of married immigrant women perceived by their home visiting educator. Methods: Data were collected using in-depth interviews and structured questionnaires during July 1st to 28th, 2009. 5 HVEs participated in focus group interview, and 27 HVEs in the questionnaire survey. Data were analyzed by content analysis and descriptive statistics using SPSS/WIN program. Results: Educational needs of Married Immigrant Women were conjugal and family relationship, child rearing and child health care, health management, sex education, and self-identification. There is no proper teaching materials about child rearing for HVEs. The educational needs of HVEs were physical and cognitive characteristics of child development, developmental screening skill and play according to the each developmental stages of the multi-cultural families. Conclusion: Family relationship, child rearing and child health care, must be stressed in educational program for Married Immigrant Women. To maximize the effectiveness of support for multi-cultural families, it must be developed a systematic educational program and teaching materials according to family developmental stages.

• Child Health Nursing Research
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• v.12 no.1
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• pp.5-14
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• 2006

Purpose: The purpose of study was to describe and evaluate the educational status regarding clinical practicum for child health nursing to facilitate student's clinical compliance for the bachelor's degree in Korea. Methods: The study was a descriptive study and included data from 40 institutions among the 53 university nursing programs in Korea(75.5% response rate). Data were collected using mailed semi structured questionnaires and content analysis was done. Results: Findings show that most institutions have common learning objectives for the clinical practicum; neonatal care, high risk infant care, hospitalized child care, and advanced nursing practice. The mode for theoretical credits in child health nursing was 5 to 6 and 3 to 4 credits for clinical practice. The practice settings were prepared to provide diverse experiences, including childcare centers, and community centers with various learning activities. Evaluation for learning outcomes included faculty and instructors. It was pointed out that updating evaluation based on student and faculty feedback is important for a comprehensive practicum evaluation. Conclusions: Findings suggest that there is a -need for a generalized curriculum for clinical practicum and for the expanding role of advanced nursing practice-, a need for diverse clinical settings for practice, and effective guidance and learning activities. It is significantly noted that the attitude and teaching methodologies of clinical instructor's are highly important to effective clinical learning outcomes.