• Bulletin of the Korean Chemical Society
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• 제30권1호
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• pp.137-144
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• 2009

In this study, quantitative and pattern recognition analyses for the quality evaluation of Herba Epimedii using HPLC was developed. For quantitative analysis, five major bioactive constituents, hyperin, epimedin A, epimedin B, epimedin C, and icariin were determined. Analysis was carried out on Capcell pak $C_{18}$ column ($250{\time}4.6$ mm, 5 ${\mu}m$) with a mobile phase of mixture of acetonitrile and 0.1% formic acid, using UV detection at 270 nm. The linear behavior was observed over the investigated concentration range (2-50 ${\mu}g/mL;\;r_2\;>$ 0.99) for all analytes. The intraand inter-day precisions were lower than 4.3% (as a relative standard deviation, RSD) and accuracies between 95.1% and 104.4%. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of one reference sample. The RSD of intra- and inter-day variation of relative retention time (RRT) and relative peak area (RPA) of the 12 selected common peaks were below 0.8% and 4.7%, respectively. The developed methods were applied to analysis of twenty Herba Epimedii extract samples. Contents of hyperin, epimedin A, epimedin B, epimedin C, and icariin were calculated to be 0$\sim$0.79, 0.69$\sim$1.91, 0.93$\sim$9.58, 0.65$\sim$3.05, and 2.43$\sim$11.8 mg/g dried plant. Principal component analysis (PCA) showed that most samples were clustered together with the reference samples but several apart from the main cluster in the PC score plot, indicating differences in overall chemical composition between two clusters. The present study suggests that quantitative determination of marker compounds combined with pattern-recognition method can provide a comprehensive approach for the quality assessment of herbal medicines.

• KSBB Journal
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• 제21권1호
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• pp.59-67
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• 2006

본 연구에서는 2차원 형광스펙트럼의 PCA 분석을 통하여 발효 공정을 모니터링하고 PCR과 PLS과 같은 다변량 분석기법을 이용하여 공정을 모델링하였다. 재조합 대장균 E. coli 와 효모 S.cerevisiae의 발효 공정 중에 얻어진 많은 양의 2차원 형광스펙트럼 자료는 우선 PCA를 통해 축소된다. 그리고 PCA에서 주성분점수와 적재 산점도는 발효 공정의 정성적 경향을 묘사하기 위해 사용되었다. 또한, PCR과 PLS는 2차원 형광스펙트럼의 분석을 위해 사용되었으며 PLS모델이 보정과 예측 능력에서 PCR모델보다 조금 더 우수한 성능을 나타냈다. 따라서 2차원 형광스펙트럼 자료를 이용하여 생물공정을 모델링 하고자 할 때는 PCR 방법보다는 PLS 방법을 사용하는 것이 유리할 것이다.

• 한국초지조사료학회지
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• 제21권2호
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• pp.67-74
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• 2001

본 시험은 봄철 속정건조에 의한 건조효과와 건초품질을 구명하여 양질의 건초를 조제코자, 알팔파(Vernal)과 연맥(Swan)을 공시하여 건조방법(건조제, conditioning, 대구조)을 달리하여 1997년 봄 수원 축산기술연구소에서 수행되었다. 수확시기는 6월 3일로 얄팔파는 개화초기, 연액은 춤수기였으며, 화학제인 건조제는 $KCO_2$ 2%를 수확직선 처리 하였고, 물리적인 conditioning은 모델 GMR 2800 trail-type mower conditioner를 사용하였다. 포장건조후 각형 곤포를 조제하였으며, 건조의 외관평가와 사료가치는 2개월간 보관저장후 분석.평가하였고, 시기별 식물체의 수분 함량은 포장상태에서 양파망을 이용 조사하였다. 건조방법별 건조속도는 conditioning구에서 우수 하였고, 전반적으로 건조제 처리효과는 낮았다. conditioning 처리시 건초조제 가능일수는 3일 정도로 건조제 처리구나 대조구에 비해 1일 이상 포장 건조기간 단축효과가 있었다. 건초의 건물손실은 알팔파와 연맥 모두 conditioning구에서 적었으며. 달관평가에서도 conditioning구에서 가장 우수하였다. 건초의 화학성분 평가에서 사료가치는 수확당시에 비해 크게 낮았는데, 이는 건조기간중 강우(7.8mm)와 저장기간중 가용성 당류의 손실에 의한 것으로 추정되며 대조구에 비해 conditioning구에서 ADF와 NDF 함량이 다소 낮고 건물소화율은 약간 높은 경향을 보였다. 이상의 결과로서 알팔파와 본 연맥의 건초조제 시 포장 건조기간 단축과 품질향상을 위해서는 mower condition$\varepsilon$r 사용이 가장 바람직하였으며, 건조제 처리효과는 없었고, 전반적으로 알팔파 건초의 품질이 본 연맥에 비해 우수하였다.

• The Korean Journal of Ecology
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• 제14권3호
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• pp.345-360
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• 1991

Benthic macroinvertebrates were collecte in the middle reaches of the paenae stream, a tributary of th naktong river in korea, in four seasons from August 1989. To may 1990, during the study period, 125 species, 81 genera and 41 families were collected. Overall, the most dominant species was lepidostoma sp. 1 followed by heptagenia kihada, neoperla quadrata, diamesa sp. 1, ect.. the community was most diverse in autumn, However the number of collectd individuals was highest in winter. Species of chironomids including diamesa sp. 1 and mayflies including paraleptophlebia chocorata were collected exceptionally in a large number in winter. Seasonal occurrence of funtional groups such as collectors, shredders and scrapers was related with the availadlity of food sourses in the stream. The abundance of predators was relatively stable in four seasons with 17% on average. Diversity, tbi and chandler's biotic score(bs) in the paenae strem were 3.75, 14.5, and 100 on average, respectively. Based on biological and chemical indices the paenae stream was clean in the range of xeno- and oligo- saprobity.

• 농촌계획
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• 제9권2호
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• pp.1-6
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• 2003

This study was performed to investigate the degree of pollution of sediments of stream and reservoir in rural area. A series of field investigations were carried out for Kyongki-do area and chemical analysis were performed for sediment samples. It was found that some samples were heavily polluted with phenol and TPH and gave off a malodor. Soil Pollution Scores(SPSs) was determined for sediment samples. Some samples were classified to Soil Pollution Class(SPC) 2 and 3. For recycling and disposal of dredged sediments from stream and reservoirs, these polluted sediments should be carefully considered. In the environmental improvement operations of rural area, the degree of pollution of sediments of stream and reservoir are carefully investigated and suitable counterplan must be established.

• 통합자연과학논문집
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• 제9권1호
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• pp.41-61
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• 2016

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality worldwide. Phosphoinositide 3-kinases (PI3Ks) play important role in Non-Small Cell Lung Cancer. PI3Ks constitute a lipid kinase family which modulates the function of numerous substrates involved in the regulation of cell survival, cell cycle progression and cellular growth. Herein, we describe the ligand based pharmacophore combined with molecular docking studies methods to identify new potent PI3K inhibitors. Several pharmacophore models were generated and validated by Guner-Henry scoring Method. The best models were utilized as 3D pharmacophore query to screen against ZINC database (Chemical and Natural) and the retrieved hits were further validated by fitness score, Lipinski's rule of five. Finally four compounds were found to have good potential and they may act as novel lead compounds for PI3K inhibitor designing.

• 열처리공학회지
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• 제34권5호
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• pp.211-217
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• 2021

To predict mechanical properties of secondary hardening martensitic steels, a machine learning ensemble model was established. Based on ANN(Artificial Neural Network) architecture, some kinds of methods was considered to optimize the model. In particular, interaction features, which can reflect interactions between chemical compositions and processing conditions of real alloy system, was considered by means of feature engineering, and then K-Fold cross validation coupled with bagging ensemble were investigated to reduce R2_score and a factor indicating average learning errors owing to biased experimental database.

• 한국환경과학회지
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• 제27권11호
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• pp.1105-1115
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• 2018

The aim of this study was to collect crucial data for the improvement of water quality and ecosystem conservation by analyzing water samples, sediments, benthic macroinvertebrates, and fish in the Seosan reservoir. The average values of water quality parameters from 2001 to 2016 were a chemical oxygen demand of 10 mg/L, total nitrogen of 1.22 mg/L, and total phosphorus of 0.074 mg/L. Cadmium was detected in the range of 0.531-0.748 mg/kg in the reservoir sediment. Fish belonging to 6 families and 9 species were identified in the reservoir. The dominant species were Carassius auratus and Micropterus salmoides. Benthic macroinvertebrates belonging to 22 families and 28 species were identified. The ecological score of the benthic macroinvertebrate community was 15 inside the reservoir (St. 2). Micropterus salmoides, an invasive alien species, was determined to be the subdominant fish species based on the number of captures, and the presence of the invasive species, Sicyos angulatus L. and Paspalum distichum L. was confirmed among the land flora.

• Asian Journal of Atmospheric Environment
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• 제8권1호
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• pp.15-24
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• 2014

The capital city of Himalayan Country Nepal, Kathmandu Valley is surrounded by consecutive high mountains, which limits the air distribution and mixing effects significantly. It in turn generates steady air flow pattern over a year except in monsoon season. The air shed in the Valley is easily trapped by the surrounded mountains and the inversion layer formulated as the cap. The $PM_{10}$ concentration was noticeably higher than the standard level (120 ${\mu}g/m^3$) in urban and suburban area of Kathmandu valley for all seasons except monsoon period. The Valley area experiences similar wind patterns (W, WWS, and S) for a year but the Easterly wind prevails only during the monsoon period. There was low and calm wind blows during the winter season. Because of this air flow structure, the air emission from various sources is accumulated within the valley air, high level of air pollution is frequently recorded with other air polluted cities over the world. In this Valley area, brick kilns are recognized as the major air pollution source followed by vehicles. Mostly Bull Trench Kiln (BKT), Hoffman Kiln and Vertical Shaft Brick Kiln (VSBK) are in operation for brick firing in Kathmandu valley where the fuels such as crushed coal, saw dust, and natural gas are used for processing bricks in this study. Tool for the Reduction and Assessment of Chemical and Other Environmental Impacts (TRACI) was used for screening and quantifying the potential impacts of air emission from firing fuels. The total Environmental Performance Score (EPS) was estimated and the EPS of coal was approximately 2.5 times higher than those of natural gas and saw dust. It is concluded that the crushed coal has more negative impact to the environment and human health than other fuel sources. Concerning the human health and environment point of view, alternative environment friendly firing fuel need to be used for brick industry in the kiln and the air pollution control devices also need to be applied for minimizing the air emissions from the kilns.

• 한국식품조리과학회지
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• 제10권2호
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• pp.126-130
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• 1994

본 연구의 목적은 현대 사회가 급격히 발전하면서 fast-foods의 이용률이 증가하므로, fast-foods 단백질의 질적, 양적 평가를 시도하고저 하였다. 연구방법으로는 시중에서 판매되고 있는 fast-foods 19가지를 구입 하여 중량을 측정한 후 동결 건조시켜 Kjeldahl법, 아미노산 자동 분석기를 이용하여 단백질, 필수아미노산 함량을 분석하였다. 이와같이 분석된 필수아미노산 함랑들을 FAO/WHO(1973) provisional pattern와 whole e일 Pattern을 기준하여 아미노산가, 화학가를 산출한 후 제 1제한 아미노산을 산정, 비교하였다. 본 연구의 결과는 다음과 같다. 1. 조사 fast-foods중 조단백 분석시 double cheese burger, 꼬치국수, pork cutlet 등에서 함량이 높게 나타났다. 2. Fast-foods의 필수아미노산 분석시 선정된 제 1제한 아미노산은 FAO/WHO(1973) provisional pattern 및 whole e99 Pattern(1972) 기준시 모두 함유황 아미노산이 었다. 3. 2-3가지의 fast-foods를 배합하여 섭취하는 경우 단백질 함량 및 제 1제한 아미노산가를 상승시킬 수 있었다. 본 연구결과 시판 fast-foods들의 섭취시 단백질, 필수아미노산 뿐 아니라 특히, fast-foods 섭취시 부족되기 쉬운 영 양소 등을 포함한 타 영 앙소들도 고려한 종합적인 섭취 pattern을 제시하고 이를 기초로 하여 청소년들이 이해하기 쉽고, 실생활에 응용시킬 수 있는 영양 교육자료 제작이 이루어져야겠다.