• Title/Summary/Keyword: chemical binding

Search Result 1,354, Processing Time 0.022 seconds

Syntheses of Silica Gel Bound Hemin, Biliverdin, and Bilirubin

  • Park, Yong-Tae;David A. Lightner
    • Bulletin of the Korean Chemical Society
    • /
    • v.6 no.2
    • /
    • pp.112-115
    • /
    • 1985

  • 3-Aminopropylsilated silica gel bound hemin, biliverdin, and bilirubin were synthesized by reacting 3-aminopropyl silated silica gel with hemin, biliverdin and bilirubin respectively. The aspects of binding of bilirubin to amino group of 3-aminopropylsilated silica gel were studied using the above synthetic silica immobilized hemin, biliverdin and bilirubin, and oxodipyrromethene.

  • https://doi.org/10.5012/bkcs.1985.6.2.112 인용 PDF

Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex

  • Hwang, Inkyu;Kim, Kwangmi;Choi, Sojin;Lomunova, Maria
    • Molecules and Cells
    • /
    • v.40 no.1
    • /
    • pp.24-36
    • /
    • 2017

  • The stability of peptide-MHC complex (pMHC) is an important factor to shape the fate of peptide-specific T cell immune response, but how it influences on T cell activation process is poorly understood. To better understand that, we investigated various T cell activation events driven by $L^d$ MHCI loaded with graded concentrations of P2Ca and QL9 peptides, respectively, with 2C TCR Tg T cells; the binding strength of P2Ca for $L^d$ is measurably weaker than that of QL9, but either peptides in the context of $L^d$ interact with 2C TCR with a similar strength. When their concentrations required for early T cell activation events, which occur within several minutes to an hour, were concerned, $EC_{50}s$ of QL9 were about 100 folds lower than those of P2Ca, which was expected from their association constants for $L^d$. When $EC_{50}s$ for late activation events, which takes over several hours to occur, were concerned, the differences grew even larger (> 300 folds), suggesting that, due to weak binding, $L^d/P2Ca$ dissociate from each other more easily to lose its antigenicity in a short time. Accordingly, fixation of $L^d/P2Ca$ with paraformaldehyde resulted in a significant improvement in its immunogenicity. These results imply that binding strength of a peptide for a MHC is a critical factor to determine the duration of pMHC-mediated T cell activation and thus the attainment of productive T cell activation. It is also suggested that paraformaldehyde fixation should be an effective tool to ameliorate the immunogenicity of pMHC with a poor stability.

  • https://doi.org/10.14348/molcells.2017.2218 인용 PDF KSCI