• Journal of Microbiology and Biotechnology
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• v.32 no.3
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• pp.307-316
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• 2022

Yeasts play an important role in spontaneous fermentation of traditional alcoholic beverages. Our previous study revealed that a mixed-consortia of both Saccharomyces and non-Saccharomyces yeasts were responsible for fermentation of khadi, a popular, non-standardized traditional beverage with an immense potential for commercialization in Botswana. Functional characterization of isolated fermenting yeasts from mixed consortia is an indispensable step towards the selection of potential starter cultures for commercialization of khadi. In this study, we report the characterization of 13 khadi isolates for the presence of brewing-relevant phenotypes such as their fermentative capacity, ability to utilize a range of carbon sources and their ability to withstand brewing-associated stresses, as a principal step towards selection of starter cultures. Khadi isolates such as Saccharomyces cerevisiae, Saccharomycodes ludwigii and Candida ethanolica showed good brewing credentials but Lachancea fermentati emerged as the isolate with the best brewing attributes with a potential as a starter culture. However, we were then prompted to investigate the potential of L. fermentati to influence the fruity aromatic flavor, characteristic of khadi. The aroma components of 18 khadi samples were extracted using headspace solid phase micro-extraction (HS-SPME) and identified using a GC-MS. We detected esters as the majority of volatile compounds in khadi, typical of the aromatic signature of both khadi and L. fermentati associated fermentations. This work shows that L. fermentati has potential for commercial production of khadi.

• Proceedings of the Korean Fiber Society Conference
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• 1999.04a
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• pp.353-356
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• 1999

• Proceedings of the Korean Ceranic Society Conference
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• 2004.04b
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• pp.39.3-39.3
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• 2004

• Proceedings of the Korea Water Resources Association Conference
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• 1996.05a
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• pp.318-324
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• 1996

• Proceedings of the Membrane Society of Korea Conference
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• 1999.04a
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• pp.45-48
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• 1999

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2001.04a
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• pp.102-103
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• 2001

• Proceedings of the Korean Ceranic Society Conference
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• 2002.10a
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• pp.132-132
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• 2002

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.789-790
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• 2005

• Proceedings of the PSK Conference
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• 2000.10a
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• pp.259.2-259.2
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• 2000

• Proceedings of the KIEE Conference
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• 1997.11a
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• pp.245-249
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• 1997

A feasibility study on the characterization of incipient insulator failure for distribution fault prediction is presented. In this study, real distribution data was collected and analyzed to isolate incipient failure signatures or parameters which were expected to show distinct behaviors before and after failure incident. Several signal analysis methods were applied to isolate the parameters and a new strategy of analysis, the event-date concept, was also applied to find a relationship between non-harmonic and high frequency signal activities and imminent insulator failures.