• Korean Journal of Microbiology
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• v.42 no.3
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• pp.195-198
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• 2006

Lethal toxin is a critical virulence factor of anthrax. It is composed two protein: protective antigen (PA) and lethal factor (LF). PA binds to specific cell surface receptors and, forms a membrane channel that mediates entry of LF into the cell. LF is a zinc-dependent metalloprotease, which cleaves MKKs [MAPK (mitogen-activated protein kinase) kinases] at peptide bonds very close to their N-termini. In this study, we suggest application of cell-based assays in the early phase of drug discovery, with a particular focus on the use of yeast cells. We constructed MEK1 expression system in yeast to determine LF activity and approached cell-based assay system to screen inhibitors, in which the results covering the construction of LF-substrate in yeast expression vector, expression, and LF-mediated proteolysis of substrate were described. These results could provided the basic steps in design of cell-based assay system with the high efficiency, rapidly and easy way to screening of inhibitors.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.11 s.114
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• pp.1050-1057
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• 2006

This paper proposes a new predistortion linearizer with amplitude modulator and PFG(Polar Function Generator) using second order harmonic signals. This linearizer consists of PFG that combine with in-phase and quadrature-phase of second harmonic signals and amplitude modulator in main path. The predistorted third order intermodulation distortion(IMD3) signals that are generated by amplitude modulator with fundamental and PFG signals, improve a amplifier nonlinear characteristics. The proposed linearizer and amplifier have been manufactured and tested to operate in cellular base-station transmitting band$(869\sim894MHz)$. The test results show that IMD3 can be removed by more than 22.5 dB in case of CW 2-tone signals ${\Delta}f=1$ MHz, and the adjacent channel power ratio(ACPR) also can be improved by more than 8.4 dB for CDMA IS-95 1FA signals.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.41 no.4
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• pp.293-301
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• 2017

A Sub-kWe small-scale experimental test loop was manufactured to investigate characteristics of the supercritical carbon dioxide power cycle. A high-speed turbo-generator was also designed and manufactured. The designed rotational speed of this turbo-generator was 200,000 rpm. Because of the low expansion ratio through the turbine and low mass flowrate, the rotational speed of the turbo-generator was high. Therefore, it was difficult to select the rotating parts and design the turbine wheel, axial force balance and rotor dynamics in the lab-scale experimental test loop. Using only one channel of the nozzle, the partial admission method was adapted to reduce the rotational speed of the rotor. This was the world's first approach to the supercritical carbon dioxide turbo-generator. A cold-run test using nitrogen gas under an atmospheric condition was conducted to observe the effect of the partial admission nozzle on the rotor dynamics. The vibration level of the rotor was obtained using a gap sensor, and the results showed that the effect of the partial admission nozzle on the rotor dynamics was allowable.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.2
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• pp.345-352
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• 2017

In this paper, a unified potentiostat which can measure the current of both $O_2$-based and $H_2O_2$-based blood glucose sensors with low supply voltage of 1.0V has been designed and verified by simulations and measurements. Potentiostat is composed of low-voltage operational transconductance amplifier, cascode current mirrors and mode-selection circuits. It can measure currents of blood glucose chemical reactions occurred by $O_2$ or $H_2O_2$. The body of PMOS input differentional stage of the operational transconductance amplifier is forward-biased to reduce the threshold voltage for low supply voltage operation. Also, cascode current mirror is used to reduce current measurement error generated by channel length modulation effects. The proposed low-voltage potentiostat is designed and simulated using Cadence SPECTRE and fabricated in Magnachip 0.18um CMOS technology with chip size of $110{\mu}m{\times}60{\mu}m$. The measurement results show that consumption current is maximum $46{\mu}A$ at supply voltage of 1.0V. Using the persian potassium($K_3Fe(CN)_6$) equivalent to glucose, the operation of the fabricated potentiostat was confirmed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37C no.12
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• pp.1185-1194
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• 2012

This paper proposes a new terrestrial 3DTV broadcasting standard specification based on synchronization with non-real-time contents in order to overcome quality limitations of the current 3DTV services that are arisen from the limited bandwidth of the legacy broadcasting channel. In the services using the proposed specification, one view sequence of a stereoscopic video content is delivered as a non-real-time content in idle time, and the other view sequence is transmitted in real time broadcasting signal, thereafter two sequences are synchronized in a receiver for display. Thus, it is possible to provide higher-quality stereoscopic video content services than the current 3DTV services. In order to realize these services, a new mechanism is required which enables synchronization between the data that are from different transmission media and time. Therefore, this paper suggests a solution by multiplexing the synchronization signals of non-real-time contents into broadcasting signals with real-time streams together. This solution can provide a accurate synchronization mechanism by minimum updates of legacy broadcasting systems while maintaining compatibility with legacy services.

• Journal of Korean Society of Disaster and Security
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• v.11 no.1
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• pp.31-38
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• 2018

The 99.1% of small dam and most of the levees in Korea are soil dam which can be constructed with lower cost and less effort compared with ones made of concrete. However, they are so vulnerable to overflow. Sudden collapses of these strucrues lead to increase flow rate rapidly, which may cause catastrophic problems in downstream regions. In this study, the experimental study on the collapse delay effect of riprap that was laid on slope of soil levee was carried out. A prismatic rectangular open channel was used and three different sizes of the riprap were installed on slope of a scaled earth dam. A new formula for the collapse time of the levee with the installation of riprap was presented, using the previous researches and the dimensional analysis. In this process, an unsteady flow condition was considered to derive the deviation time of the riprap. And additional experiments were conducted to understand the effect of reinforcement of riprap, and it was found that the reinforcement of riprap was more effective than twice sizing of intial riprap. If the collapse time is delayed, EAP (Emergency Action Plan) and forecasting can greatly reduce the degree of flood damage. Also, it will be meaningful that the results of this study are used for river design.

• Journal of Korea Water Resources Association
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• v.44 no.1
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• pp.65-72
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• 2011

Natural streams meander, forming pools at the outer part of bend and riffles at the crossing. Pools are deep at a lower flow velocity, and riffles are shallow at a higher flow velocity. Attentions are being paid to pool-riffle sequences in meandering streams because pool-riffle sequences tend to increase biodiversity of the stream ecosystem. This study investigates the characteristics of distribution of bed sediment particles in the upstream reach of the Gap Stream, which is a tributary of the Geum River in Korea. The upstream part of the Gap Stream, the study reach, is a gravel-bed stream, showing a pool and three riffles due to meandering. The reach also includes pools at the upstream and downstream parts of the weir. The characteristics of bed sediment particles sampled at the wetland and in the side channel are studied, revealing that the median particle diameter in the riffle is about four times larger than that in the pool. In addition, flow simulations are carried out for ordinary discharge and design flood, and such parameters important to sediment transport as velocity, shear stress, dimensionless shear stress (or Shields number), and dimensionless shear velocity are provided to see the mobility of sediment particles in the pool-riffle sequence.

• Tunnel and Underground Space
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• v.20 no.2
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• pp.82-91
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• 2010

Grouting technology is one of the ground improvement methods used in water controlling and reinforcement of rock mass in underground structure construction. It is necessarily required to find out the characteristics of grout flow through discontinuities in a rock mass for an adequate grout design and performance assessment. Laminar flow is not always applicable in simulating a grout flow in a rock mass, since the rock joints usually have apertures at a micro-scale and the flow through these joints is affected by the joint roughness and the velocity profile of the flow changes partially near the roughness. Thus, the influence of joint roughness and aperture on the grout flow in rough rock joint was numerically investigated in this study. The commercial computational fluid dynamics code, FLUENT, was applied for this purpose. The computed results by embedded Herschel-Bulkley model and VOF (volume of fluid) model, which are applicable to simulate grout flow in a narrow rock joint that is filled with air and water, were well compared with that of analytical results and previously published laboratory test for the verification. The injection pressure required to keep constant injection rate of grout was calculated in a variety of Joint Roughness Coefficient (JRC) and aperture conditions, and the effect of joint roughness and aperture on grout flow were quantified.

• Journal of Korea Multimedia Society
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• v.6 no.2
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• pp.340-351
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• 2003

We introduce our experiences of the design and implementation of the Page Together system that has expanded hyperlink metaphor to utilize human resources in the web. This system supports that a user connects with others in the web, communicates through video/audio channel, navigates same web pages simultaneously and cooperates some work on Internet. For these functions, it comprises Collaborative Browsing Module (CBM), Multimedia Conferencing Module(MCM) Data Conferencing Module(I)CM) and Multi Protocol Interface(MPI). We adopted three standard protocols, IEC, H.323 and T.120 for each nodule and it allows developers to use them easily. We also defined MPI to synchronize information of session among modules. Each module exchanges information each other in session creating process and session terminating process. After a session is created once, each module works independently as its won protocol. Interferences among modules are reduced as minimizing to exchange information. We also introduce a web site that provides web board service based on the Page Together system. A user may post a notice with a link to himself/herself on our web board. After then, if someone read that notice and has any question about it, he or she can try to connect to the writer as clicking the link in that notice and communicate each other. This service site shows that our system can be applied to diverse internet services such as distance teaming and distance conference.

• Journal of Distribution Science
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• v.14 no.4
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• pp.55-66
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• 2016

Purpose - This article aims to explore the characteristics of disaster risk distribution information in China. Also, this research attempts to analyze the findings of risk communication using case study in chronological order in terms of social amplification of risk. To achieve the purpose, the paper reviews the trends and issues of risk communication in China, with an emphasis on examining earthquakes by a chronological approach. In these regards, we hope that some relevant findings from this empirical study with cases will be able to enhance national risk communication and provide implications in Korea as well. Research design, data, and methodology - The conceptual framework of this study is theoretically based on the risk amplification model, which describes signals about risk transmitted and processed by individuals and social groups. The social amplification of risk also reflects the interactions of social groups about disaster-related risk issues, which are potential amplifiers or attenuators of communication signals. The key concept of social amplification implies that the risks pertaining to natural disasters interact with social, psychological, institutional, and cultural processes in ways that can affect public perceptions of risk. SMCRE Model is methodologically employed to examine risk communication history of China with the focus on natural disaster. Four earthquakes are selected to figure out the chronological characteristics of risk communication since 1970s. He bei Tang Shan earthquake is selected as an example disaster before 1990's, while the earthquake in Yun Nan Jiang is explored for the case study of 1990's. The earthquake in Si Chuan Wen Chuan is also examined as a example disaster of 2000's. The recent earthquake in Si Chuan Ya An Lu Shan is selected as a case of 2010s. Results - SMCRE model in this case study is operationally defined as a methodology and applied to the four earthquakes occurred in China. SMCRE model describes the exchange of risk information and is also applied to all forms of communication between stake holders. Each factor of risk communication includes source, message, channel, receiver and effect. It is notable that a big progress has been made on disaster risk communication in China for the past 40 years. We also found that highly developed information technology has enabled Chinese society to better cope with natural disaster, leading to enhanced disaster risk communication. It is mainly found from case study that the disaster risk communication of China has been involved with political situation, which derived from the change of government for the past 40 years. Conclusion - From this historical research, it can be inferred that the policies and politics of Chinese leaders have had a more critical role to play in the process of source of risk communication than those of any other countries. The results of this paper also support that the effective risk communication involves not only the improved reliability of local government as a key factor of disaster risk communication, but also is accompanied by international cooperation for substantial collaboration with stake holders.