• 대한의생명과학회지
• /
• 제14권3호
• /
• pp.179-186
• /
• 2008

Matrix metalloproteinases (MMPs), also designated matrixins, hydrolyze components of the extracellular matrix. These proteinases playa central role in many biological processes, such as embryogenesis, normal tissue remodeling, wound healing, and angiogenesis, and in diseases such as atheroma, arthritis, cancer, and tissue ulceration. In previous data, disruption of the actin cytoskeleton by cytochalasin D (CD) inhibited NO-induced apoptosis, dedifferentiation, cyclooxygenase (COX)-2 expression, and prostaglandin $E_2$ production in chondrocytes cultured on plastic or during cartilage explants culture. In this study, we investigated the effects of the actin cytoskeleton architecture on MMP-2 expression and dedifferentiation by CD in rabbit articular chondrocytes. Rabbit articular chondrocytes were prepared from cartilage slices of 2-weeks-old New Zealand white rabbits by enzymatic digestion. CD was used as a disruptor of actin cytoskeleton. In this experiments measuring CD dose response, primary chondrocytes were treated with various concentrations of CD for 24h. The actin disruption was determined by immunostaining. MMP-2 expression levels were determined by immunoblot analysis and Reverse transcriptase-Polymerase chain reaction (RT-PCR) and MMP-2 activity was determined by gelatin zymography. We found that cell morphological change and up-regulation of MMP-2 expression by CD as determined via immunostaining, gelatin zymography and immunoblotting. Moreover, CD induced MMP-2 transcription was detected by RT-PCR. Also, CD-induced type II collagen expression was inhibited by MMP-2 inhibitor I treatment. Our results indicate that CD up-regulated MMP-2 activation causes dedifferentiation of articular chondrocyte.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권10호
• /
• pp.1508-1513
• /
• 2006

Myostatin (MSTN) and insulin-like growth factors (IGFs) are a known inhibitor and stimulators of proliferation and differentiation of muscle cells, respectively. The present study was performed to investigate the relationship of MSTN-induced growth inhibition to expression of the IGF system components and myogenin, a muscle cell-specific transcription factor, in rat L6 myoblasts. The L6 cells transfected with a green fluorescent protein-MSTN plasmid expression construct had a 47% less cell number than mock-transfected cells after 3-d serum-free culture, accompanied by delayed differentiation which was suggested by inhibited aggregation of cells. Moreover, cells transfected with the expression construct had decreased expression of IGF-II and myogenin genes, but not IGF-I or its receptor genes, as examined by reverse transcription-polymerase chain reaction. The reduced mitosis of the L6 cells transfected with the MSTN-expression construct increased following an addition of either IGF-I or IGF-II to the culture medium, but not to the level of mock-transfected cells. By contrast, myogenin gene expression in these cells increased after the addition of either IGF to the level of mock-transfected cells. Collectively, these results suggest that the inhibitory effect of MSTN on L6 cell proliferation and differentiation is likely to be partly mediated by serially suppressed expression of IGF-II and myogenin genes, not IGF-I gene.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권9호
• /
• pp.1516-1524
• /
• 2018

Objective: Defensins are a large family of antimicrobial peptides and components of the innate immune system that invoke an immediate immune response against harmful pathogens. Defensins are classified into alpha-, beta-, and theta-defensins. Avian species only possess beta-defensins (AvBDs), and approximately 14 AvBDs (AvBD1-AvBD14) have been identified in chickens to date. Although substantial information is available on the conservation and phylogenetics, limited information is available on the expression and regulation of AvBD8 in chicken immune tissues and cells. Methods: We examined AvBD8 protein expression in immune tissues of White Leghorn chickens (WL) by immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (RT-qPCR). In addition, we examined AvBD8 expression in chicken T-, B-, macrophage-, and fibroblast-cell lines and its regulation in these cells after lipopolysaccharide (LPS) treatment by immunocytochemistry and RT-qPCR. Results: Our results showed that chicken AvBD8 protein was strongly expressed in the WL intestine and in macrophages. AvBD8 gene expression was highly upregulated in macrophages treated with different LPS concentrations compared with that in T- and B-cell lines in a time-independent manner. Moreover, chicken AvBD8 strongly interacted with other AvBDs and with other antimicrobial peptides as determined by bioinformatics. Conclusion: Our study provides the expression and regulation of chicken AvBD8 protein in immune tissues and cells, which play crucial role in the innate immunity.

• 대한한의학회지
• /
• 제28권2호통권70호
• /
• pp.54-65
• /
• 2007

Objectives : In the present study, the author investigated whether Naenghyo-hwan(NHH) significantly affect mucin secretion from airway epithelial cells. Methods : Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of NHH to assess the effect of the agent on 3H-mucin secretion. Total elutionprofiles of control spent media and treatment sample through Sepharose CL-4B column were analysed. Effect of NHH on contractility of isolated tracheal smooth muscle was investigated. Also, effect of the agent on MUC5AC gene expression in cultured NCI-H292cells was investigated. Possible cytotoxicities of the agent were assessed by measuring both lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells. Results : NHH significantly increased mucin secretion from cultured HTSE cells, with significant cytotoxicity. NHH chiefly affected the 'mucin' secretion. NHH inhibited ACh-induced contraction of isolated tracheal smooth muscle. NHH disturbed both the extraction of total RNA from NCI-H292 cells and polymerase chain reaction, nonspecifically. Therefore, in this experiment, theeffect of NHH on the expression levels of MUC 5AC gene in cultured NCI-H292 cells could not be elucidated. Conclusions : The author suggests that the effect of NHH with their components should be further investigated and it is valuable to find, from oriental medical prescriptions, novel agents which might regulate mucin secretion from airway epithelial cells.

• 디지털산업정보학회논문지
• /
• 제12권1호
• /
• pp.1-12
• /
• 2016

The current economic crisis is making new demands on manufacturing industry, in particular, in terms of the flexibility and efficiency of production processes. This requires production and administrative processes to be meshed with each other by means of IT systems to optimise the use and capacity utilisation of machines and lines but also to be able to respond rapidly to wrong developments in production and thus to minimise adverse impacts on the business. The future scenario of the "smart factory" represents the zenith of this development. The factory can be modified and expanded at will, combines all components from different manufacturers and enables them to take on context-related tasks autonomously. Integrated user interfaces will still be required at most for basic functionalities. The complex control operations will run wirelessly and ad hoc via mobile terminals such as PDAs or smartphones. The comnination of IoT, and Big Data optimisation is bringing about huge opportunities. these processes are not just limited to manufacturing, anywhere a supply chain environment exists can benefit from information provided by linked devices and access to big data to inform their decision support. Building a smart factory with smart assets at its core means reaching those desired new levels of productivity and efficiency. It means smart products that leverage advanced traceability, connectivity and intelligence. For businesses, it means being able to address the talent crunch through more autonomous. In a Smart Factory, machinery and equipment will have the ability to improve processes through self-optimization and autonomous decision-making.

• 대한수의학회지
• /
• 제40권1호
• /
• pp.86-93
• /
• 2000

Paratuberculosis (Johne's disease), a chronic enteritis produced by Mycobacterium paratuberculosis, affects a large proportion of ruminants in all continents and causes important economic losses. The identification of well-characterized and species-specific components of M paratuberculosis would provide the means to improve the specificity and sensitivity of immunodiagnostic assays for Johne's disease. The aims of this study were to express the recombinant C-terminal of 34kDa protein (rC34P) of M paratuberculosis in E coli and to investigate the effectiveness of this protein in detecting antibodies to the native protein in sera from paratuberculosis infected cattle. The C-terminal of the gene encoding the 34kDa protein was amplified by polymerase chain reaction from the chromosomal DNA of M paratuberculosis (ATCC 19698) and cloned into vector pGEX-4T-2. Then, cloned plasmid was transformed into E coli DH5${\alpha}$ and the rC34P was overexpressed. The rC34P was purified by affinity chromatography and gel filtration. The rC34P was examined antigenicity by Western blot. The rC34P was reactive with culture positive bovine serum and hyperimmune rabbit anti-M paratuberculosis serum but was not reactive with culture negative bovine serum and tuberculin positive bovine serum in Western blot. In conclusion, the rC34P produced in this study is expected as a useful candidate for antigen in serological diagnosis of Johne's disease.

• 한국구조물진단유지관리공학회 논문집
• /
• 제16권5호
• /
• pp.29-39
• /
• 2012

상관관계가 높은 복합열화의 완벽한 개별예측모델의 개발은 매우 어려운 문제로, 본 논문에서는 현수교 시스템의 미래열화와 유지 예산을 예측하기 위하여, 10년간의 유지 데이터가 주어진 매개변수(파손지표와 사용성)의 사후 확률 밀도함수를 찾기 위해 베이지언 추론을 적용하였다. 마르코프 연쇄 몬테카를로법을 이용하여 매개변수의 사후 분포를 조사하였다. 감소한 사용성의 모의위험예측은 사전분포와 연간유지 업무에서 업데이트한 데이터의 가능성에 따라 작성한 사후 분포이다. 기존의 선형 예측 모델과 비교하면, 제안된 2차 모델은 교량부품의 사용성, 위험요소, 그리고 유지 예산의 측정 데이터에 대하여 매우 개선된 수렴성과 근접성을 제공한다. 따라서 제안된 2차 추계학적 회귀 모델을 기반으로 복잡한 사회간접설비의 미래 성능과 유지관리예산을 예측하고 제어할 수 있는 기회를 제공할 것으로 기대한다.

• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 2000년도 Proceedings of 2000 KSAM International Symposium and Spring Meeting
• /
• pp.150-156
• /
• 2000

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. The 30 fatty acids which were identified or characterized are classified. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Cluster 3 and 4-a strains were containing Q-8, these strains are accommodated in the Proteobacteria gamma and beta subdivision. The chemotaxonomic profiles of the cluster 4-a strains showed good agreement with those of the genus Burkholderia. Cluster 3 was characterized by the presence of branched-chain fatty acids, iso-C15:0, iso-C17:1, and iso-C17:0 as the major components. These chemotaxonomy suggested the close relationship of the isolates with Xathomonas/Sterotrophomonas group. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Sterotrophomonas, but were not included in these genera. These strains were even further away from core Xanthomonas, and clearly were seen to branch outside the cluster formed by the Sterotrophomonas maltophilia. MH256 and MA828 16S rDNA sequence was different enough to put new genus on a separate branch. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium.

• 스마트미디어저널
• /
• 제1권2호
• /
• pp.12-22
• /
• 2012

최근 비디오 감시, 로봇 시각 휴대폰 등 무수히 많은 카메라가 생활 속에 파고들면서 휴먼 동작 인식은 컴퓨터 시각 분야의 새로운 붐을 일으키고 있다. 자체로 그다지 흥미 있는 동작은 아니지만 걸음걸이 또는 보행은 가장 보편적으로 많이 관찰되는, 의심할 여지없이 사람의 대표적인 동작이다. 그리 오래되지 않은 과거에 보행자 인식의 관점에서 반짝 연구가 있었지만 관심의 길이가 짧은 만큼 보행 동작에 관한 체계적인 분석과 이해 없이 이루어졌었다. 본 연구에서는 일련의 점진적인 모델을 이용하여 보행 동작의 구조를 체계적으로 분석하고자 한다. 입력 영상 신호의 다양한 변형과 불완전성을 극복할 수 있는 동적 베이스망 기반의 보행자 모델과 보행 모델을 제시한다. 그리고 이변량 폰 미제스 분포의 조건부 밀도 함수를 기반으로 마르코프 체인의 이산 상태 공간을 연속 공간으로 확장하는 방법을 제안한다. 제안된 모형화 프레임워크를 이용한 일련의 시험, 분석에서 보행자를 91.67% 인식하며 보행 동작을 보행 방향과 보행 자세의 두 가지 독립적인 성분으로 분리 해석할 수 있었다.

• 한국유통학회지:유통연구
• /
• 제13권3호
• /
• pp.127-154
• /
• 2008

본 논문은 고급화된 대형마트 즉 '한국형 할인점'이라는 독특한 업태 컨셉에 보다 적합한 서비스 품질 측정 적도를 연구하는데 그 목적이 있다. 이를 위해 반복적 FGI와 대형 할인점 방문 소비자 604명을 대상으로 정량적 리서치를 실시하였다. 연구 결과 '한국형 할인점'의 서비스 품질은 본원적 혜택, 프로모션, 인적상호작용, 물리적 측면, 정책, 부가적 편의성의 총 6개 차원, 24개 항목으로 이루어진 측정모형이 도출되었다. 기존의 소매 서비스 품질의 대표적 측정 모형인 RSQS 측정모형과 비교해 보면 3가지 자원(인적상호작용, 물리적 측면, 정책)은 공통적이며, 나머지 차원에서 문제해결은 인적상호작용으로 통합되었으며, 신뢰성은 단일차원성이 결여되어 제거되었다. 특히 본원적 혜택은 할인점의 업태특성을 대표하는 성과품질로서 도출되었으며, 정책 자원은 '재방문 의도'에 가장 큰 영향을 미치는 중요한 서비스 품질 자원으로 확인되었다. 본 연구에서 수정, 보완된 측정도구는 한국형 할인점을 이용하는 고객의 최신 트랜드를 반영하여 업버전 하였다는 것이 그 특징이다. 그 결과 향후 소매 시장 포화상황에서 치열한 서비스 경쟁을 벌여야하는 한국의 대형마트와 기타 소매업체에 전략적 시사점을 주고 있다.