• 동의생리병리학회지
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• 제21권5호
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• pp.1341-1345
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• 2007

There are four kinds of formulas for purging the liver to cure its sthenic syndrome based on the types of preparation formulas : Sagan-tang, Saganhwan, Saseem-san and Saganeum. Another formula called Sacheonghwan, Sacheong-tang and Sacheong-san is to purge the green colour of liver. There are 38 kinds of Sagan-tang, 2 kinds of Saganhwan, 29 kinds of Sagan-san, 5 kinds of Saganeum, 4 kinds of Sacheonghwan, 3 kinds of Sacheong-tang and 1 kind of Sacheong-san. Combination of herbal medicines, carried out in formulas for purging the liver, consists of various kinds depending on medical scientists' personal experience in medical treatment without any general principles, which makes it difficult to apply it to clinical use. The objectives of this study lie in theoretical establishment of Sagan-tang for curing the sthenic syndrome of liver through analyzing the component medicines and combination principles of Hyeongok's Sagan-tang, and furthermore, maximizing the clinical use of Sagan-tang. This study analyzed the component medicines and combination principles of Hyeongok's Sagan-tang based on the theory for properties and tastes of herbal medicines from the ${\ulcorner}$Yellow Emperor's Canon of Internal Medicine${\lrcorner}$, the theory for principal herbal medicine, assistant herbal medicine, adjuvant herbal medicine, dispatcher herbal medicine, and the five elements doctrine. Hyeongok's Sagan-tang is an odd prescription, composed of 7 kinds of ingredients : No.1 Radix Paeoniae (2don;7.5 g), No.2 Fructus Chaenomelis (1don;3.75 g), No.3 Radix Rehmanniae (1don), No.4 Folium Phyllostachydis Henonis (1don), No.5 Radix Bupleuri (1don), No.6 Radix Scutellariae (1don), and No.7 Radix Glycyrrhizae (1don). There are three methods for curing the sthenic syndrome of liver according to the five elements doctrine : purging the liver, purging the heart and invigorating the lung. In the case of taste purgation, two herbal medicines with sour taste, Radix Paeoniae and Fructus Chaenomelis, are combined into the principal and assistant herbal medicine, respectively. For property purgation, two herbal medicines with the cool property, Radix Bupleuri and Radix Scutellariae, are combined into adjuvant herbal medicines. Both sweet and cold herbal medicines, Radix Rehmanniae and Folium Phyllostachydis Henonis, are combined into adjuvant herbal medicines. Sour herbal medicines, Radix Paeoniae and Fructus Chaenomelis, were combined to invigorate the lung. Cool herbal medicines, Radix Bupleuri and Radix Scutellariae, were combined to invigorate the lung and to purge the liver. In addition, Radix Glycyrrhizae are combined as dispatcher herbal medicine, harmonizing all the herbal medicines composing the formula. First, to cure the sthenic syndrome of the liver, the methods of purging the liver and the heart, and invigorating the lung should be used according to the five elements doctrine. Secondly, herbal medicines appropriate for those treatment methods should be chosen according to the theory for properties and tastes of herbal medicine and thirdly, the combination of those herbal medicines should be carried out according to the theory for principal herbal medicine, assistant herbal medicine, adjuvant herbal medicine, dispatcher herbal medicine. As a good example, Hyeongok's Sagan-tang is combined according to the above theories.

• 대한한의정보학회지
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• 제11권1호
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• pp.26-47
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• 2005

In this dissertation, I will focus on the channel entry, the effect, and the treatment throughout books of oriental medicine from ancient to modern in order to classify the medicines of the liver as main or supplementary organ. The results are as follows: 1. The kinds of the medicines of working of the liver(本臟) were 29, which were Amydae carapax(鱉甲), Chrysanthemi Flos(菊花), Cassiae Torae Semen(草決明), Plantaginis Semen(車前子), Scirpi Seu Sparganii(三稜), Ulmi Pasta Semen(蕪荑), Cinnamomi Cortex(肉桂), Fraxini Cortex(秦皮), Chaenomelis Fructus(木果), Corii Asini Colla(阿膠), Chuanxiong Rhizoma(川芎), Adenophorae Radix(沙蔘), Coicis Semen(薏苡仁), Acanthopanacis Cortex(五加皮), Zizyphi Spinosae Semen(酸棗仁), Picrorrhizae Rhizoma(胡黃連), Gentianae Radix(草龍膽), Citri Reticulatae Virdie Pericarpium(靑皮), Paeoniae Alba Radix(白芍藥), Paeoniae Rubra Radix(赤芍藥), Bupleuri Radix(柴胡), Peucedani Radix(前胡), Naturalis Indigo(靑黛), Citrus unshiu(橘葉), Rhinocerotis Cornu(犀角), Aucklandiae Radix(木香), Polygonati Odorati Rhizoma, Farfarae Flos(款冬花), Evodiae Fructus(吳茱萸), Citri Reticulatae Pericarpium(陳皮) . 2. The kinds of the medicines of working of other viscera(他臟) were 7, which were Astragaliadix(黃耆), Ginseng Radix(人蔘), pinelliae Rhizoma(半夏), Myristicae Semen, Euryales Semen, Arecae Semen, Piperis Longi Fructus. 3. Medicines, effected on the heart functioned through any other viscera are as follows: Arecae Semen works to treat Spleen Gi Entering the Liver(脾氣入肝), Piperis Longi Fructus, pinelliae Rhizoma(半夏), Euryales Semen and Myristicae Semen operate to treat Spleen Cold Entering the Liver(脾冷入肝), Astragali Radix(黃耆) and Ginseng Radix(人蔘) work to treat Spleen Vacuity Entering the Liver(脾虛入肝). In the study of concerning the medicines effected on the liver, It is considered that it dedicated to development of the medicines related to the disease of the liver and making efficient use of the medicines.

• 한국식품과학회지
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• 제25권5호
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• pp.530-534
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• 1993

전통기호음료성분의 기능특성을 조사하기 위한 연구의 일환으로 결명자, 들깨, 대추, 모과, 오미자, 오갈피 및 생강 추출물을 여러가지 획분으로 분획하여 아질산염 소거작용을 조사한 결과, 본 실험에 사용한 시료는 모두 아질산염 소거작용이 있었으며, 수용성 획분을 에탄올 가용성 획분이 에탄올 침전획분으로 분획하였을 때, 결명자, 들깨, 오갈피, 생강의 경우에는 에탄올 가용성 획분이 에탄올 침전획분보다 아질사염 소거작용이 뛰어났으며 대추, 모과, 오미자의 경우에는 그 반대의 경향을 나타내었다. 시료 추출물 중에 함유되어 있는 환원성 물질의 관여도 클 것으로 생각되어 전자공여작용을 조사해 본 결과, 기호음료 원료에서 추출한 수용성 획분의 전자공여 작용은 오갈피>들깨>모과>생강>대추>오미자>결명자의 순으로 나타났으며, 에탄올 침전획분과 에탄올 가용성 획분의 전자공여효과도 아질산염 소거효과와 경향이 일치하였다.

• 사상체질의학회지
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• 제15권3호
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• pp.216-220
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• 2003

We treated a Taiyangin(太陽人) patient with Yulkyuk(?膈) disease. He was diagnosed as acute cerebellar infarction, hypertension and trigeminal neuralgia(rule out). His chief complaint was nausea, dysphagia, trigeminal neuralgia, anorexia, and numbness of upper and lower extremity. He was orally administrated with Mihudeung Sikjang Tan(??藤植腸湯) additions. Mihudeung Sikjang Tang(??藤植腸湯) additions was Fructus Chaenomelis(木瓜), Grape root(葡萄根), Buckwheat(蕎麥), Semen pruni(櫻桃肉) etc. His chief complaint was improved by oral administration of Mihudeung Sikjang Tan(??藤植腸湯) additions, and we do report this clinical case study.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.386.1-386.1
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• 2002

The H2O and 50% extracts of herbal medicines(HM) combinations which were consisted of Acanthopanacis Cortex. Phragmitis Rhizoma. Chaenomelis Fructus. Pruni pseudocerasi Semen and rice bran were prepared and administered orally before 40% ethanol administration in the males S.D rats. The 50% ethanol extract of HM (HM50E) showed blood alcohol decreasing activity and was fractionated again into HM50El and E2 by Sephadex LH-20 gel column chromatography. HM50E2 showed more effective blood ethanol decreasing activity than HM50El. (omitted)

• 동의생리병리학회지
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• 제19권3호
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• pp.710-715
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• 2005

This research was to investigate inhibitory activity of Sasang medicines on Obesity. 56 kind of herb medicines to powder, abstract add 100 times methanol. Examine inhibitory effect against lipase activity in vitro. It was compared Xenical(Orlistat) with 56 kind of herb medicines. Inhibitory effect against lipase activity of Xenical as a standard was 93.3%. It is Inhibitory effect against lipase activity of Taeyangin herb medicine. (Fructus Pruni Tomentosae 77.7% Cortex Acanthopanacis73.5%, Fructus Chaenomelis73.1%, rice bran on a mallet head68.1%, Semen Fagopyri 48.1%, Radix Vitis 31.5%) It is Inhibitory effect against lipase activity of Soyangin herb medicine. (Radix Saposhnikoviae 91.5%, Semen Plantaginis 90.4%, Semen Trichosanthis 89.2%, Herba Schizonepetae 85.7%, Radix Euphorbiae Kansui 76.1%, Rhizoma Anemarrhenae 76.0%, Cortex Phellodendri 75.1%, Herba Menthae 74.8%, Radix Angelicae Pubescentis 61.2%, Rhizoma Alismatis 62.6%, Poria 60.9%, Rhizoma Notopterygii 22.5%, Radix Peucedani 18.2%, Caulis Akebiae 17.7%) It is Inhibitory effect against lipase activity of Taeeumin herb medicine. (Herba Spirodelae 91.4%, Radix Polygalae 88.3%, Arillus Longanae 84.3%, Radix Platycodi 81.7%, Semen Zizyphi Spinosae 80.2%, Spina Gleditsiae 79.7%, Herba Ephedrae 75.3%, Semen Raphani 73.1%, Rhizoma Cimicifugae 73.0%, Rhizoma Acori Graminei 71.8%, Flos Chrysanthemi71.0%, Radix et Rhizoma Rhei 68.4%, Vermiculus Holotrichia 59.9%, Radix Puerariae 55.3%, Fructus Schizandrae 53.4% Semen Coicis 50.6%, Semen Biotae 47.5%, Semen Nelumbinis 46.7%, Radix Angelicae Dahuricae 45.1%, Semen Castaneae 44.4%, Rhizoma Ligustici 34.5%, Tuber Liriopis 16.4%, Radix Scutellariae4.2%) It is Inhibitory effect against lipase activity of Soeumin herb medicine. (Pericarpium Arecae89.9%, Rhizoma Alpiniae Officinarum 89.5%, Radix Polygoni Multiflori81.2%, Rhizoma Cyperi 79.0%, Cortex Magnoliae 72.8%, Radix Aucklandiae 72.0%, Rhizoma Zingiberis 71.9%, Pericarpium Citri Reticulatae Viride 71.3%, Radix Cynanchi Wilfordi 57.7% Rhizoma Pinelliae 36.7%, Fructus Alpiniae Oxyphyllae 35.8% Fructus Aurantii Immaturus 17.5%, Pericarpium Citri 16.1%) Therefore, Radix Saposhnikoviae(91.5) and Herba Spirodelae(91.4%) were the most effective medicice of 56 kind of medicines.

• 대한한의학방제학회지
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• 제16권1호
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• pp.117-130
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• 2008

Korean traditional medicine has been used for the treatment of the various diseases based on both oriental medicinal theory and clinical trials. Thus, the prescriptions of Korean traditional medicine would be useful for the development of new therapeutics. This research focuses on the fundamental study in Korean traditional prescriptions for the development of new hepatoprotective agents. We found two prescriptions. Injinho-Tang and Osumogwa-Tang, showed the significant DPPH free radical scavenging and hepatoprotective effect, respectively. It is well-known that free radical scavenging effect is related to the prevention of various pathological events including liver injury. This paper deals with hepatoprotective effects on tacrine-induced cytotoxicity in Hep G2 cells, free radicals scavenging on both DPPH and superoxide of above two prescriptions. Hot water extract of Injinho-Tang did not show the significant hepatoprotective effect on tacrine-induced cytotoxicity in Hep G2 cells, however, it shows the significant scavenging effects for both DPPH and superoxide radicals. On the other hand, all of the hot water extracts of constituent herbal drugs in Injinho-Tang exhibited the promising protective effect on tacrine-induced cytotoxicity in Hep G2 cells. Of these, water extract of Rhei Rhizoma showed the most prominent effect on tacrine-induced cytotoxicity in Hep G2 cells. Bioassay-guided fractionation of Rhei Rhizoma extract has furnished four compounds, and their chemical structures have been identified by comparison of their spectral data with those of literature as chrysophanol (1), emodin (2), 3,5-dihydroxy-4'- methoxystilbene (3), and rhapontigenin (4), respectively. Among the isolated compounds, compounds 2-4 revealed the significant hepatoprotective effect in vitro when their $EC_{50}$ values compare with that of silybin, as a positive control. It also exhibited that emodin possessed the most hepatoprotective effect among these active compounds. In case of Osumogwa-Tang, its hot water extract showed the moderate protective effect on tacrine-induced cytotoxicity in Hep G2 cells. Hot water extract of Chaenomelis Fructus, one of the constituent herbal drug of this prescription, exhibited the significant hepatoprotective effect with $EC_{50}$ value of $7.8{\pm}0.1\;{\mu}g/ml$, however, it showed strong cytotoxicity in Hep G2 cells above the concentration of $25\;{\mu}g/ml$. It was revealed that both hot water extract of Evodiae Fructus and its butanol soluble fraction showed the moderate hepatoprotective effect but concentration-dependent activity in Hep G2 assay system. Two quinolone alkaloids, evocarpine and dihydroevocarpine, also tested for their hepatoprotective effects on tacrine-induced cytotoxicity in Hep G2 cells, however, these two compounds derived from the Evodiae Fructus as the major constituents did not show in vitro hepatoprotective effect. From these results, it would be necessary to further isolation of its hepatoprotective compounds from the butanol soluble fraction of the hot water extract of Evodiae Fructus.

• KSBB Journal
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• 제18권4호
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• pp.282-288
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• 2003

본 연구에서는 항염증 및 항산화 활성을 갖는 유용한 물질 을 검색하기 위해 40여 가지 생약재를 에탄올 추출하여 H HAase 억제 및 라디칼 소거 활성을 측정하였다 .. HAase 억제 활성을 측정한 결과, I mg/mL의 농도에서 황기, 두충, 오미 자, 황금, 오가피, 목과, 목단피, 산사가 50% 이상의 저해활 성을 나타내었고, 이들에 대하여 n-hexane, chloroform, ethyl a acetate, water로 순차적으로 용매분획하여 활성을 측정한 결 과, 모든 시료의 ethyl acetate와 water 분획에서 높은 활성을 보였다 항산화 활성은 DPPH 라디칼에 대한 전자공여능과 F Fenton 반응에 의해 발생한 hydroxyl radical에 대한 소거능으 로 평가하였다 .. DPPH 라디칼에 대한 전자공여능은 복분자, 목단피, 백작약, 차전자, 마가목 에탄올 추출물이 I mg{mL 놓도에서 90% 이상으로 대조군으로 이용한 BHA와 Q - -tocopherol과 유사한 활성을 나타내었다 .. 2-deoxyribose 산화 법에 따른 hydroxyl 라디칼 소거활성에서 대부분의 에탄올 추출물 시료에서 모두 높은 활성을 나타내었다. 활성올 갖는 시료에 대하여 지질과산화 억제 활성을 측정한 결과 linoleic a acid와 phosphatidylcholine liposome 기질 모두에 대하여 오미 자 추출물은 가장 높은 억제 활성을 나타내었다.

• 대한본초학회지
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• 제30권3호
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• pp.19-24
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• 2015

Objectives : HPL-1, a novel herbal medicine which is composed of five herbs such as Kalopanacis Cortex, Chaenomelis Fructus, Raphani Semen, Atractylodis Rhizoma and Pulvis Aconiti Tuberis Purificatum, was developed for treatment of osteoarthritis. This study is aimed to develop analytical method for consistent quality control of HPL-1 and validate chromatographic method. Methods : Chromatographic analysis was performed using ultra-high performance liquid chromatography - diode array detector (UHPLC-DAD) equipped with RP-amide column, column oven, and auto sampler. Marker compounds [protocatechuic acid, chlorogenic acid, liriodendrin, 3,5-dicaffeoylquinic acid, ${\beta}$-D-(3-O-sinapoyl)-fructofuranosyl-$\alpha$-D-(6-O-sinapoyl)glucopyranoside and benzoylmesaconine] were separated by step gradient elution of acetonitrile and 0.1% phosphoric acid/water. The method validation was evaluated by quantitative validation parameters of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) according to KFDA guideline.Results : An optimized method for six marker compounds in HPL-1 was established by UHPLC-DAD. The correlation coefficient (R²) with each calibration curve was greater than 0.99. The LOD and LOQ were within the range of 0.008-0.090 and $0.023-0.274{\mu}g/mL$, respectively. The relative standard deviation (RSD) of intra- and inter-day variability were less than 4.0%. The result of recovery test was range from 93.3-106.3% with RSD < 4.0%.Conclusions : These results suggest that the quantitative UHPLC method is precise, accurate, effective for quality evaluation of HPL-1. The method may also contribute to improve quality of crude drug preparations used for treatment of various diseases.

• 대한한의학방제학회지
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• 제16권2호
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• pp.91-100
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• 2008

Background and Objectives : There are three kinds of formulas for purging the kidney to cure its sthenic syndrome based on the types of preparation formulas : Sashintang, Sashinhwan and Sashinsan. There are 4 kinds of Sashintang, 1 kind of Sashinhwan and 1 kind of Sashinsan. Combination of herbal medicines, carried out in formulas for purging the kidney, consists of various kinds depending on medical scientists' personal experience in medical treatment without any general principles, which makes it difficult to apply it to clinical use. The objectives of this study lie in theoretical establishment of Pyeongshintang for curing the sthenic syndrome of kidney through analyzing the component medicines and combination principles of Hyeongok's Pyeongshintang, and furthermore, maximizing the clinical use of Pyeongshintang. Methods : This study analyzed the component medicines and combination principles of Hyeongok's Pyeongshintang based on the theory for properties and tastes of herbal medicines from the "Yellow Emperor's Canon of Internal Medicine", the theory for principal herbal medicine, assistant herbal medicine, adjuvant herbal medicine, dispatcher herbal medicine, and the five elements doctrine. Hyeongok's Pyeongshintang is an odd prescription, composed of 7 kinds of ingredients : No.1 Rhizoma Alismatis (2don;7.5g), No.2 Poria (1don;3.75g), No.3 Radix Paeoniae Alba (1don), No.4 Fructus Chaenomelis (1don), No.5 Polyporus (1don), No.6 Cortex Cinnamomi (1don), and No.7 Radix Glycyrrhizae (1don) Results : There are three methods for curing the sthenic syndrome of kidney according to the five elements doctrine : purging the kidney, purging the liver and invigorating the spleen. First, if you suffer from the sthenic syndrome of the kidney, you need to purge your kidney. There are two available methods, including taste and property purgation according to the theory for properties and tastes of herbal medicines. They each imply the salty taste and the hot property purge the kidney. In the case of taste purgation, two herbal medicines with salty taste, Rhizoma Alismatis and Poria, are combined into the principal and assistant herbal medicine, respectively. For property purgation, two herbal medicines with the hot property, Polyporus and Cortex Cinnamomi, are combined into adjuvant herbal medicines. Secondly, if you suffer from the sthenic syndrome of the kidney, you need to purge your liver which is child in the mother-child relationship in inter-promotion among the five elements. There are two methods to purge the liver, including taste and property purgation according to the theory for properties and tastes of herbal medicines. They each mean the sour taste and the cool property purge the liver. Therefore, it is important to use sour herbal medicines for taste purgation and cool ones for property purgation. Both sour and cool herbal medicines, Radix Paeoniae Alba and Fructus Chaenomelis, are combined into adjuvant herbal medicines. Lastly, if you suffer from the sthenic syndrome of the kidney, you need to invigorate your spleen which is an element being surpassed in the relationship between the elements surpassed and ones not surpassed in inter-restraint among the five elements. There are two methods to invigorate the spleen, which include taste and property invigoration according to the theory for properties and tastes of herbal medicines. Taste invigoration means to invigorate the spleen with sweet taste and property invigoration to invigorate the spleen with warm property. Therefore, it is important to use sweet herbal medicines for taste invigoration and warm ones for property invigoration. Both sweet and warm herbal medicines, Polyporus and Cortex Cinnamomi, were combined to invigorate the spleen and purge the kidney. In addition, Radix Glycyrrhizae are combined as dispatcher herbal medicine, harmonizing all the herbal medicines composing the formula. Conclusions : First, to cure the sthenic syndrome of the kidney, the methods of purging the kidney and the liver, and invigorating the spleen should be used according to the five elements doctrine. Secondly, herbal medicines appropriate for those treatment methods should be chosen according to the theory for properties and tastes of herbal medicine and thirdly, the combination of those herbal medicines should be carried out according to the theory for principal herbal medicine, assistant herbal medicine, adjuvant herbal medicine, dispatcher herbal medicine. As a good example, Hyeongok's Pyeongshintang is combined according to the above theories. In conclusion, this formula was created by applying to the theory for properties and tastes of herbal medicines.