• 한국동물위생학회지
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• 제15권1호
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• pp.7-16
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• 1992

In order to detection of the Intestinal parasites, 503 fecal samples were taken from mongorel-and pad-dogs in Chonbuk province. The prevalence and identification of intestinal parasites were determined by the fecal examinations using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows 1. Fifty-nine percent (297 dogs) from 503 fecal samples were detected eggs. In seasonal detection rate of eggs, Summer was 30.3%, Autumn 26.4%, Winter 22.3% and Spring 21.0%, in order, 2. A total of 20 kinds of eggs were isolated from feces, and it was identified 75.7% as Nematoda(320 dogs), 5.6% as Cestoda(24 dogs) and 1.4% as Trematoda(6 dogs), and 17.2% as Protozoa(73 dogs). The isolates were identified as Ancylostoma caninum (30.4%, 153 dogs), Isospora spp. (14.3%, 72 dogs), Toxocara canis(11.1%, 56 dogs), Toxascaris leonina(5.8%, 29 dogs) , Uncineria stenocephala or Physaloptera spp. (5.4%, 27 dogs), Trichuris vulpis(2.4%, 12 dogs) and the others, single or in combination. 3. In mixed infection such as single, double, triple and quadraple was 63.6%, 31.7%, 3.4% and 1.3%, respectively.

• Parasites, Hosts and Diseases
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• 제58권4호
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• pp.457-460
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• 2020

The Pacific tapeworm Adenocephalus pacifcus (syn. Diphyllobothrium pacificum) is a causative agent of diphyllobothriosis occurred in Pacific coast of South America, mainly in Peru. Source of infections are traditional meal from raw or undercooked marine fish such as "cebiche". We confirmed 3 new cases, one including scolex and the other two headless. A strobila 46 cm long without scolex was discharged from an 8-year-old boy before treatment. Specimens were confirmed morphologically by presence of tegumental protuberances on proglottids and small sized eggs. Partial sequence of cytochrome c oxidase subunit 1 gene was congruent with A. pacificus sequences.

• Parasites, Hosts and Diseases
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• 제54권3호
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• pp.363-368
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• 2016

We describe here the mass death of predatory carp, Chanodichthys erythropterus, in Korea induced by plerocercoid larvae of Ligula intestinalis as a result of host manipulation. The carcasses of fish with ligulid larvae were first found in the river-edge areas of Chilgok-bo in Nakdong-gang (River), Korea at early February 2016. This ecological phenomena also occurred in the adjacent areas of 3 dams of Nakdong-gang, i.e., Gangjeong-bo, Dalseong-bo, and Hapcheon-Changnyeong-bo. Total 1,173 fish carcasses were collected from the 4 regions. To examine the cause of death, we captured 10 wondering carp in the river-edge areas of Hapcheon-Changnyeong-bo with a landing net. They were 24.0-28.5 cm in length and 147-257 g in weight, and had 2-11 plerocercoid larvae in the abdominal cavity. Their digestive organs were slender and empty, and reproductive organs were not observed at all. The plerocercoid larvae occupied almost all spaces of the abdominal cavity under the air bladders. The proportion of larvae per fish was 14.6-32.1% of body weight. The larvae were ivory-white, 21.5-63.0 cm long, and 6.0-13.8 g in weight. We suggest that the preference for the river-edge in infected fish during winter is a modified behavioral response by host manipulation of the tapeworm larvae. The life cycle of this tapeworm seems to be successfully continued as the infected fish can be easily eaten by avian definitive hosts.

• Parasites, Hosts and Diseases
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• 제56권1호
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• pp.87-91
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• 2018

Morphological and molecular characteristics of spirometrid tapeworms, Spirometra decipiens, were studied, which were recovered from a heavily infected stray cat road-killed in Eumseong-gun, Chungcheongbuk-do (Province), the Republic of Korea (=Korea). A total of 134 scolices and many broken immature and mature proglottids of Spirometra tapeworms were collected from the small intestine of the cat. Morphological observations were based on 116 specimens. The scolex was 22.8-32.6 mm (27.4 mm in average) in length and small spoon-shape with 2 distinct bothria. The uterus was coiled 3-4 times, the end of the uterus was ball-shaped, and the vaginal aperture shaped as a crescent moon was closer to the cirrus aperture than to the uterine aperture. PCR amplification and direct sequencing of the cox1 target fragment (377 bp in length and corresponding to positions 769-1,146 bp of the cox1 gene) were performed using total genomic DNA extracted from 134 specimens. The cox1 sequences (377 bp) of the specimens showed 99.0% similarity to the reference sequence of S. decipiens and 89.3% similarity to the reference sequence of S. erinaceieuropaei. In the present study, we report a stray cat heavily infected with S. decipiens identified by mitochondrial cox1 sequence analysis and morphological examinations of the adult worms.

• Parasites, Hosts and Diseases
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• 제54권6호
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• pp.777-786
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• 2016

Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.

• 한국동물위생학회지
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• 제30권1호
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• pp.175-181
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• 2007

Parasites cause great economic loss in livestock in Korea, and can be categorized as either or indirect losses. In order to monitor the parasites from October 2005 to January 2006, 401 samples were randomly collected from cattle farms and examined the prevalence of gastro-intestinal (GI) parasites by using the sucrose flotation method and formalin-ether centrifugation method. The overall infection rate of GI parasites was about 43.4%. Of these parasites, nematodes were identified as Capillaria bovis (5.5%), Strongyloides papillosus (2.5%), Trichuris discolor (1.2%). Cestoda was observed only one species, Moniezia benedeni (2.2%). The rate of Eimeria spp was 39.2%. A total of five species of Eimeria was identified; among the species identified as above E bovis and E zuernii appeared with relatively higher infection rates. The combined infection of parasites was 35.2% in single species, 29.0% in double and 2.0% in triple infections. No cases of clinical symptoms were observed in this survey. The EPG levels of each species of parasites were very low in all infected cases.

• Parasites, Hosts and Diseases
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• 제22권1호
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• pp.85-95
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• 1984

한국 시장에서 얻은 명태의 장내 기생 조충류 조사의 일환으로 이 연구를 실시하였다. 명태의 장에서 얻은 각종 충체에 대하여 형태학적 계측과 박절표본을 작성한 소견을 가지고 Schmidt와 Yamaguti의 검색표에 의거하여 속과 종을 동정한 바 다음과 같은 결과를 얻었다. 1. 본 실험에서 국내에 미기록된 신종으로 Eucestoda 아강 Caryophyllidae목 Caryophyllaeidae과 Lytocesinae아과 Khawa속과 Eucestoda아강 Trypanorhyncha목 Acystidea아목 Paranybeliniidae과 Pseudonybelinia속과, Eucestoda아강 Trypanorhyncha목 Aeystidea아목 Tentaculariidae과 Nybelinia속과 Tentacularia속 그리고 Eucestoda아강 Trypanorhyncha목 Cystidae아목 Lacistorhynchidae과 Eulacistorhynchus속과 이들의 종을 확정하였다. 2. Khawia sinesis의 특징은 두절에 소포가 없으며 넓고 펑평하나 가장자리가 들쑥날쑥한 점과 목이 짧고 체부와 두부사이가 명화하지 않은 점이었다. 3. Eulncistorhynchus chiloscyllius의 특징은 두절이 꽤 긴 편이며 가장자리가 들쑥날쑥하지 않다. 구문낭도 길다. 수축근은 남의 기저부에 부착되어 있다. 흡구는 둘로 난형 혹은 원형이며 후단에, 절흔이 없다. 촉모는 길 갈고리들이 변형되어 있으며 이중의 chainette가 있다. 체절은 acraspedote하고 성숙체질은 체폭보다 길이가 더 길다. 4. Tentacularia coryphaenea의 특징은 두절이 들쑥 날쑥하지 않고 원주형이다. 흡구는 분리되었고 후단 유리 연에는 작은 가시가 있다. 두절에서 나온 4개의 수축성 촉모에는 갈고리들이 나선형으로 배열되어 있다. 촉모를 둘러싼 초는 꼬여있지 않다. 근낭은 긴 타원형이다. 5. Pseudonybelinia odontacantha의 특징은 두절이 들쑥날쑥하지 않고 촉모는 계구의 전쳔부에 가까이 부착되어 있다. 갈고리는 좌측으로 부터 나선상으로 배열되어 있다. 근낭은 폭보다 길이가 3배정도 긴다. 6. Nykelinia lingu구곤의 특징은 두절이 짧고 들쑥 날쑥하지 않다 흡구는 4개로 분리되어 있으며 각기 자유판을 갖고 있다. 촉모는 원통상으로 단단한 갈고리로 무장되어 있다.

• 생태와환경
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• 제49권3호
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• pp.215-227
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• 2016

담수생태계에서 어류기생충의 종류와 감염경로는 다양하고, 중형 또는 고등동물을 숙주로 하여 생활환을 이어가기 때문에 매우 복잡하다. 그중에서 어류의 복강에 기생하는 충류를 대상으로 수행되었다. 본 연구는 2007년 2월부터 10월까지 섬강에서 생긴 피라미의 기생충에 관한 민원을 해결하고자 생태계의 감염특성을 조사하였다. 섬강에서 복강기생충이 관찰된 시기에 감천, 대포천, 양산천 및 율하천도 동일하게 조사하였다. 저서성대형무척추동물은 수서곤충류와 패류가 각각 90.4%, 7.7%로써 주종을 이루었다. 수서곤충류는 하루살이류 (46.8%)와 날도래류 (29.8%)가 대부분을 차지하였고, 패류는 복족류였다. 어류는 잉어과와 피라미 (Zacco platypus)가 각각 85.7%, 48.8%로써 가장 풍부하였다. 물새 조류는 흰뺨검둥오리 (Anas poecilorhyncha)와 쇠백로 (Egretta garzetta)가 높은 밀도로 우점하였다. 어류기생충은 복강에 주로 기생하는 편형동물문 조충류 Ligula intestinalis로써 분류 동정되었다. 10월에 섬강과 양산천에서 우점 서식한 피라미로부터 각각 1개체, 15개체 관찰되었다. 복강기생충의 감염률은 1.4~15.8% 범위였다. 수서생태계의 조사결과로부터 기생충-숙주 관계의 감염 생활사를 고찰하였다. 본 연구결과는 국내의 육수학적 관점에서 복강기생충의 생태를 이해하는 데 유용한 기초자료로써 활용되기를 기대한다.

• 대한의생명과학회지
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• 제11권2호
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• pp.153-163
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• 2005

To clarify the species validity of the genus Spirometra, the biological characteristics of Spirometra erinacei and S. mansonoides by developmental stages were compared. Their experimental life cycles were maintained under the same laboratory conditions, and the biological characteristics were experimentally observed in vivo and in vitro conditions. Eggs of S. erinacei and S. mansonoides were $59.6\pm35.6{\mu}m\;and\;61.4\pm35.8{\mu}m$ in each average size. Both of them became fully matured and hatched in 8 days after incubation at $29^{\circ}C$. The coracidium of S. erinacei was $43.6\times35.8{\mu}m$ in average size, and retained a oncosphere of $39.3\times31.0{\mu}m$. That of S. mansonoides was $43.0\times36.3{\mu}m$ in average size, and retained a oncosphere of $38.3\times30.8{\mu}m$. Procercoids of S. erinacei were somewhat larger than those of S. mansonoides. Both species of procercoids older than 7 days in cyclops had minute spines at the anterior end, calcium corpuscles in the parenchyme and a cercomer at the posterior end. The procercoids older than 4 days in cyclops were infective to tadpoles. The procercoids older than 8 days revealed the infectivity to mice. Plerocercoids of S. erinacei were somewhat lager than those of S. mansonoides when they were compared by age of worms in tadpoles. Both species of plerocercoids older than 5 days were infective to mice. Among 138 plerocercoids of S. erinacei recovered from the experimental mice, $55(39.9\%)$ were detected in the neck portion, $35 (25.4\%)$ in the back portion, $25(18.1\%)$ in the anterior legs, and $23 (16.7\%)$ were found in the abdomen. In case of S. mansonoides plerocercoids, $42.0\%$ were found in the neck portion, $23.8\%$ in the back portion, $14.4\%$ in the abdomen, $13.3\%$ in the anterior legs, and $6.1\%$ were found in the posterior legs. From the above results, it was confirmed that the biological characteristics of S. erinacei and S. mansonoides are almost same when their life cycles are mainteined under the same laboratory condition. Accordingly, these findings suggest that S. erinacei and S. mansonoides may be the same species.

• 한국임상수의학회지
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• 제32권3호
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• pp.239-242
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• 2015

오징어에 기생하는 Nybelinia surmenicola의 충미충 감염상을 조사하고 광학 및 주사전자현미경을 적용하여 종을 동정 하였다. 대전 어물전에서 시판하는 총 200 마리의 오징어를 검사하여 54 마리 (27%)에서 N. surmenicola의 충미충을 검출하였다. 양성 오징어의 충미충 출현빈도는 2.28이였다. 충체의 길이와 폭은 $6.1-11.1(8.05){\times}2.0-2.2(2.1)mm$이였고 흡구는 $2.4-2.8{\times}0.62-0.69(2.65{\times}0.64)mm$로 계측되었다. 문 (tentacule)은 돌출되어 있으며 사선으로 16개의 갈고리가 무장되어 있었다. 문초(tentacle sheaths)는 bulb 가까이에서 1회 꼬여 있었다. 흡구 가장자리에 사상의 microtriches가 존재하였다. 4개의 거대한 흡구는 가장자리에서 사상모양의 microtriches에 의해 분리되었다. 본 논문은 한반도 연안 오징어에서 N. surmenicola 충미충 검출의 최초 보고이다.