• Animal cells and systems
• /
• 제11권2호
• /
• pp.227-234
• /
• 2007

Spermatogenesis, the reproductive cycle, and the size at first sexual maturity in male Mactra chinensis were investigated by cytological and histological observations. The spermatozoon exhibits a primitive type morphology and is similar to those of other bivalves in that it contains a short midpiece with four mitochondria surrounding the centrioles. The morphologies of the sperm nucleus type and the acrosome shape of this species are cylindrical and modified cap-like, respectively. The spermatozoon is approximately $40-45\;{\mu}m$ in length including the sperm nucleus (about $1.46\;{\mu}m$), acrosome (about $1.20\;{\mu}m$) and tail flagellum. The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. The spawning period of this species lasts from June to September, and the main spawning occurs in July and August, when the seawater temperature is greater than $20^{\circ}C$. The percentage of individual male clams at first sexual maturity was 56.5% for those whose shell lengths were 35.1-40.0 mm, and 100% for over 45.1 mm. Accordingly, harvesting clams <35.1 mm in shell length could potentially cause a drastic reduction in recruitment, and a measure indicating a prohibitory fishing size should be taken for adequate fisheries management.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2005년도 추계학술대회 논문집
• /
• pp.535-538
• /
• 2005

The purpose of this work is to study a process organization on space cognition by visio-auditory stimulation. We develop the system of visuo-auditory stimulation and Humans responses measurement to observe the relationship between the sensory and the motor system fur the localization of visual and auditory target direction in the space. The experiments is performed in a soundproof chamber, 2163 red, green and yellow LED(Luminescent Diode, Brightness: $20cd/m^2$ 1 degree apart each other)arrayed in front of half-circle panel were used and 57 Speaker(5 degree apart each other) arrayed in the hidden of half-circle panel. Physiological parameters such as EOG (Electro-Oculography), head movement and their synergic control are measured by BIOPAC system and Optotrak Certus. This result shows that the response latency time of the perception motion in the center is laster than the periphery of panel. These results can be used in the study of characterizing the spatial cognition.

• 공학교육연구
• /
• 제25권5호
• /
• pp.33-43
• /
• 2022

This study started from a critical review of the current problem of ethics education in engineering colleges, how it has become stereotyped and perfunctory and hence, is failing to properly foster engineers with a sense of responsibility. Existing engineering ethics education occupies a very limited role and weight in the periphery of the engineering curriculum, unable to hold a central position. This situation is of grave concern, especially when the leadership of engineers with social and professional responsibility is of great importance. Therefore, this study explored new directions and strategies for engineering ethics education while reviewing domestic and foreign studies dealing with the current status and problems of engineering ethics education. First, this study discussed the need for and direction of shifting to ethical engineering education instead of ethics education centered on liberal arts courses. Second, this study presented specific practical strategies to construct the ethical engineering curriculum. For example, it is necessary to establish the ethical engineering curriculum with an education linking ethics with human development goals, ethics education using Korea's current issues and agendas, micro- and macro-level analysis related to engineering, and leadership education.

• 대한기계학회논문집B
• /
• 제23권10호
• /
• pp.1310-1319
• /
• 1999

High-pressure swirl injectors have usually been employed in Gasoline direct injection engines due to their spray characteristics and the feasibility of their control. Thus the microscopic characteristics of high-pressure swirl spray were investigated by PDA. The correlation between axial and radial velocities and the correlation between droplet size and axial velocity were examined with different axial and radial positions. Two dimensional droplet velocity and its number distribution with size-classified droplets were illustrated. The mean droplet velocity and its SMD were also analyzed at the center of spray, the position having maximum mean axial velocity, and the spray periphery using time dividing method. Finally, the structure of high-pressure swirl spray was presented with the size distribution and velocity profile of droplets.

• 한국수산과학회지
• /
• 제28권6호
• /
• pp.736-752
• /
• 1995

Fine structural changes of germ cell during the gametogenesis of Korean rockfish, Sebastes schlegeli sampled in west coast of Korea were investigated from September 1993 to August 1994. In a layer of microvilli of oocyte with active yolk duplication, many pinocytotic vesicles containing protein granules regarded as yolk precursors were observed. The multivesicular bodies were formed by gathered mitochondria. They are participated in formation of the primary yolk globules homogeneously filled with high dense particles and enclosed within a limiting membrane. The precursors of yolk globule appeared to be formed by modification of mitochondria and they developed into the primary yolk globules with participation of large and dense pinocytotic vesicles. Yolk globules in mature oocyte were consisted of three components: the crystalline type main body, the superficial layer with dense and fine granules, and the limiting membrane. Steroid hormone secreting cells were recognized in the interstitial cells of growing testis. Numerous endoplasmic reticula and large mitochondria with well developed tubular cristae appeared in their cytoplasms. The axoneme in the tail flagellum of spermatozoon consisted of nine pairs of microtubules at the periphery and one pair at the center, and they were covered with doublet microtubules.

• Design & Manufacturing
• /
• 제15권3호
• /
• pp.26-31
• /
• 2021

In this study, laser-induced periodic surface structure (LIPSS) was fabricated on Ni, Si, and GaAs samples using a flat-top beam with a uniform energy distribution that was fabricated using a Gaussian femtosecond laser with a mechanical slit and tube lens. Unlike the Gaussian beam, the flat-top beam has a uniform beam profile, therefore the center and the periphery of the fabricated LIPSS have similar line periodicity. In addition, LIPSS was obtained not only in metals but also in metalloids and metals and metalloid compounds by using the narrow pulse width characteristic of a femtosecond laser.

• 한국패류학회지
• /
• 제22권2호
• /
• pp.115-124
• /
• 2006

Gonad index, spermatogenesis and the reproductive cycle of Meretrix petechialis were investigated by cytological, histological observations. Monthly changes in the gonad index coincides the gonadal development. The morphology of the spermatozoon had a primitive type and is similar to that of other bivalves having a short mid-piece with five to six mitochondria surrounding the centrioles. The morphology of the sperm nucleus type and the acrosome shape of this species were cylindrical type and cap-like shape, respectively. The spermatozoon was approximately 40-45 ${\mu}m$ in length including the sperm nucleus length (about 1.50 ${\mu}m$), acrosome length (0.60 ${\mu}m$) and tail flagellum. The axoneme of the tail flagellum consisted of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail showed 9 + 2 microtubular arrangement. The spawning period was from June to September and the main spawning occurred from July to August when seawater temperatures were higher than $20^{\circ}C$. The reproductive cycle of this species could be categorized into five successive stages: early active stage (February to March), late active stage (February to May), ripe stage (April to July), partially spawned stage (June to September), and spent/inactive stage (September to February).

• 한국발생생물학회지:발생과생식
• /
• 제20권3호
• /
• pp.247-254
• /
• 2016

Cytological changes of the epithelial cells according to the developmenatal phases of the seminal vesicle related to the spermatogenic stages in the testicular lobules during spermagenesis in male Neptunea (Barbitonia) cumingii (Gastropoda: Buccinidae) were investigated monthly by electron microscopical and histological observations. N. (B) cumingii is dioecious, and an internal fertilization species. The male genital organ is located near the tentacles. The spermatozoon is approximatley $50{\mu}m$ in length. The axoneme of the tail flagellum consists of nine pairs of microtubles at the periphery and one pair at the center. The process of germ cell development during spermatogenesis can be divided into five succesive stages: (1) spermatogonia, (2) primary spermatocytes, (3) secondary spermatocytes, (4) spermatids, and (5) spermatozoa. A considerable amount of spermatozoa make their appearance in the testicular lobules (or acini) and some of them are tranported from the testis towards the seminal vesicles until late July. In this study, the developmental phases of the epithelial cells of the seminal vesicles of N. (B.) cumingii could be classified into four phases: (1) S-I phase (resting), (2) S-IIphase (early accumulating), (3) S-III phase (accumulating), and (4) S-IV phase (spent). However, in case of N. (B.) arthritica cumingii, the developmental phases of the seminal vesicle were devided into three phases: (1) resting, (2) accumulating and (3) spent. Granular bodies in the inner layer of the seminal vesicles are involved in resorption of digestion of residual spermatozoa.

• BMB Reports
• /
• 제32권1호
• /
• pp.92-97
• /
• 1999

Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.

• ALGAE
• /
• 제22권2호
• /
• pp.95-106
• /
• 2007

Blooms of Chattonella species are normally during summer in inland seas with high nutrients from the land and inflowing water. These blooms cause mass fish kills worldwide. We isolated a Chattonella strain from the south coast of Korea and identified it as C. antiqua. It is known that the morphological changes of phytoplankton correspond to the diurnal vertical migrations that follow an intrinsic biological clock and a nutrient acquisition mechanism during the day and night. In electron micrographs, C. antiqua clearly showed a radial distribution of lipid bodies in subcellular regions and plastids composed in which thylakoid layers were perpendicular to the surface. A single pyrenoid was present in each plastid and it was found at the end of the plastid towards the center of the cell. Throughout the day, plastids of C. antiqua cells appeared as an expanded net-like recticulum. During the night, however, the plastids changed their shape and contracted toward the cell periphery. The electron density of pyrenoids was increased in cells harvested during the night.