• Title/Summary/Keyword: cellulose-degradation

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Impact of Three Categories of Supplements on In Sacco Ruminal Degradation of Urea-Treated and Untreated Straw Substrates

  • Srinivas, Bandla;Krishnamoorthy, U.;Jash, Soumitra
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.2
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    • pp.195-204
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    • 2002
  • The objective of this study was to examine the impact of three categories of supplements on intake and diet induced difference on degradation of straw substrates. Sixteen crossbred cattle fitted with rumen cannula were randomly divided into 4 equal groups. Animals were fed on wheat straw ad libitum without any supplement except mineral mixture (control; $T_1$) or supplemented with concentrate mixture (CS; $T_2$) or green Lucerne (GLS; $T_3$) or urea-molasses block lick (ULS; $T_4$). Total dry matter intake in $T_2$, $T_3$ and $T_4$ was increased by 70, 54 and 49%, respectively compared to $T_1$ which was only 1.55 kg/100 kg B.Wt. Other than control animals, straw intake was less on $T_3$ than $T_2$ or $T_4$. In Sacco degradation of untreated and urea treated wheat or paddy straw in different treatments indicated that the supplements had a significant (p<0.01) impact on rapidly soluble (A) and insoluble but potentially degradable (B) fractions of straw. Urea treatment increased fraction-A but, provision of supplement improved fraction-B also. Effective degradation (ED) of OM was better on $T_2$. Rate of degradation (C) of OM and CWC was dependent on diet and type of straw but hemicellulose and cellulose were related to latter factor only. ED of cell wall carbohydrates (CWC) was similar in $T_2$ and $T_4$ but higher than $T_3$. CS was more effective in improving the degradation of both untreated and urea treated straw while ULS was effective on the former only. CS had more impact on superior quality straw while contrary was true with ULS. Although GLS improved intake and degradability of untreated and urea treated straws, its bulkiness affected the straw intake compared to other supplements.

The Effect of Saturated Fatty Acids on Cellulose Digestion by the Rumen Anaerobic Fungus, Neocallimatix frontalis C5-1

  • Ha, J.K.;Lee, S.S.;Gao, Z.;Kim, C.-H.;Kim, S.W.;Ko, Jong Y.;Cheng, K.-J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.7
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    • pp.941-946
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    • 2001
  • The effects of various concentrations of saturated fatty acids (SFA; caprylic, capric and stearic acids) on the growth of the anaerobic fungus, Neocallimastix frontalis C5-1 isolated from the rumen of a Korean native goat were investigated. At higher concentrations of fatty acids (0.1%, w/v), the addition of SFA strongly decreased filter paper (FP) cellulose digestion and polysaccharide-degrading enzyme activity. The sensitivity of the rumen anaerobic fungus to the added fatty acids increased in the following order: caprylic ($C_{8:0}$)>capric($C_{10:0}$)>stearic($C_{18:0}$) acid, although stearic acid had no significant (p<0.05) inhibitory effects at any of the concentrations tested. However, the addition of SFA at lower concentrations (0.01 and 0.001% levels), did not inhibit FP cellulose degradation and enzyme activity. Furthermore, although these parameters were slightly stimulated by the addition of SFA, they were not statistically different from control values. This is the first report examining the effects of fatty acids on anaerobic gut fungi. We found that the lower levels of fatty acids used in this experiment were able to stimulate the growth and specific enzyme activities of rumen anaerobic fungi, whereas the higher levels of fatty acids were inhibitory with respect to fungal cellulolysis.

Effects of LCFA on the Gas Production, Cellulose Digestion and Cellulase Activities by the Rumen Anaerobic Fungus, Neocallimastix frontalis RE1

  • Lee, S.S.;Ha, J.K.;Cheng, K.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.8
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    • pp.1110-1117
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    • 2001
  • Responses of the rumen fungus, Neocallimastix frontalis RE1, to long chain fatty acid (LCFA) were evaluated by measuring gas production, filter paper (FP) cellulose digestion and polysaccharidase enzyme activities. LCFA (stearic acid, $C_{18:0}$; oleic acid, $C_{18:1}$; linoleic acid, $C_{18:2}$ and linolenic acid, $C_{18:3}$) were emulsitied by ultrasonication under anaerobic condition, and added to the medium. When N frontalis RE1 was grown in culture with stearic, oleic and linoleic acid, the cumulative gas production, gas pool size, FP cellulose digestion and enzymes activities significantly (p<0.05) increased at some incubation times(especially, exponential phases of fungal growth, 48~120 h of incubation) relative to that for control cultures. However, the addition of linolenic acid strongly inhibited all of the investigated parameters up to 120 h incubation, but not after 168 and 216 h of incubation. These results indicated that stearic, oleic and linoleic acids tended to have great stimulatory effects on fungal cellulolysis, whereas linolenic acid caused a significant (p<0.05) inhibitory effects on the cellulolysis by the rumen fungus. These results are the first report of the effect of LCFAs on the ruminal fungi. Further research is needed to identify the mode of action of LCFAs on fungal strains and to verify whether or not ruminal fungi have ability to hydrate unsaturated LCFAs to saturated FAs. There was high correlation between cumulative in vitro gas production and fungal growth (94.78%), FP cellulose degradation (96.34%), CMCase activity(90.86%) or xylanase activity (87.67%). Thus measuring of cumulative gas production could be a useful tool for evaluating fungal growth and/or enzyme production by ruminal fungi.

Effect of Biological and Liquid Hot Water Pretreatments on Ethanol Yield from Mengkuang (Pandanus artocarpus Griff)

  • Yanti, Hikma;Syafii, Wasrin;Wistara, Nyoman J;Febrianto, Fauzi;Kim, Nam Hun
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.2
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    • pp.145-162
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    • 2019
  • This study aimed to increase the sugar and ethanol yield from the mengkuang plant biomass through biological and liquid hot water (LHW) pretreatment and their combination. The results showed that biological pretreatments with 5% inoculum of the fungus Trametes versicolor resulted in the highest alpha cellulose content incubated for 30 days, and 10% inoculum resulted in the lowest lignin content. LHW pretreatment decreased the hemicellulose content of pulps from 10.17% to 9.99%. T. versicolor altered the structure of the mengkuang pulp by degrading the lignin and lignocellulose matrix. The resulting delignification and cellulose degradation facilitate the hydrolysis of cellulose into sugars. The alpha cellulose content after biological-LHW pretreatment was higher (78.99%) compared to LHW-biological pretreatment (76.85%). Scanning electron microscopy analysis showed that biological-LHW combinated treatment degrades the cell wall structures. The ethanol yield for biological-LHW pretreated sample was observed 43.86% (13.11 g/L ethanol by weight of the substrate, which is much higher than that of LHW-biological pretreatment (34.02%; 9.097 g/L). The highest reducing sugar content about 45.10% was observed with a resulting ethanol content of 15.5 g/L at LHW pretreatment temperature of $180^{\circ}C$ for 30 min.

Factors Affecting on the biodegradation of cellulose fibers

  • Park, Chung-Hee;Kang, Yeon-Kyung;Im, Seung-Soon
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.10a
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    • pp.15-16
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    • 2003
  • When textiles are buried in soil, soil resident microorganisms take part in degradation of textile material, which is called biodegradation, and the biodegradability is often used as a standard for the environment-friendliness of textile products.(omitted)

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A Study on the Mechanical Properties of Shrink-Resistant Wool Fabric Treated with Enzyme (효소에 의한 방축가공 양모직물의 물성 변화에 관한 연구)

  • 박미라;김환철;박병기
    • Textile Coloration and Finishing
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    • v.13 no.3
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    • pp.155-164
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    • 2001
  • Textile wet-processing industry usually five rise to environmental pollution problems caused by using chemical substance. The objective of this study is to apply enzymes for wool and reduce the environmental problems. Three commercial protein degradation enzymes and a cellulose degradation enzyme as a reference were treated to prevent the shrinkage of wool fabric on laundering. Shrink resistant effects used change with the kinds of enzyme, the amount of enzyme, assistant chemicals, and the pre-treatment condition of wool fabric. When pre-treated with corona before enzyme treatment under ultrasonic condition, the weight loss was increased and strength was decreased and elongation was increased. Both corona pre-treatment and the addition of $Na_2SO_4$ also decreased shrinkage of wool fabrics on laundering. The existence of assistant chemicals increased alkali solubility of wool fabrics.

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Effect of several carbohydrates on lignin degradation by pleurotus ostreatus (Pleurotus ostreatus에 의한 리그닌 분해에 미치는 수종 탄수화물의 영향)

  • 김규중;맹진수;강사욱;하영칠;홍순우
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.323-328
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    • 1986
  • To clarify the effects of several carbohydrates on the biodegradation of lignin by Pleurotus ostreatus. The strain was cultured on the media formulated with lignin and carbohydrates such as cellulose, xylan, collobiose, glucose and xylose, which was added individually. The culture mixtures grown 36 days were filtered and then estimated the degree of lignin biodegradation. It was found that the growth of P. ostreatus was stimulated and the depoly-merization was also increased by the addition of carbohydrates. When the carbohydrates were not added, polymerization was apparent in stead of depolymerization. In the case of glucose as an added carbohydrate, the content of lignin by the nitrosolignin method was greatly (about 7.4 times) decreased than control which contains lignin as a carbon source. The peak of lignin at 280nm in UV spectra was decreased about 27% after 27 days of culture. As results, it was assumed that lignin biodegradation was correlated to the carbohydrates and especially glucose was very significant role in lignin degradation.

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COMPARATIVE STUDY OF FIBER CHARACTERISTICS OF RICE AND WHEAT STRAWS

  • Zhiliang, T.;Huiping, C.;Tingxian, X.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.1
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    • pp.51-56
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    • 1996
  • In this Experiment three wether male Matou goats (♂), all fitted with permanent rumen fistulae, were used to study the rumen degradabilities (incubation time 48h) of dry matter (DM), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent ligin (ADL), cellulose (CEL) and hemicellulose (HC) and their contents of wheat and rice straws were determined for the different morphological fractions and segments. The results showed that the variation of different fractions for wheat and rice straw is the true resources of their nutritive value variation and the cell wall contents of wheat and rice straw are also obviously different. The rumen degradabilities of different straw fibre are significantly different (p<0.01), mean while the effect of straw variety is also significant (p<0.05). The aim was to set up a foundation for studying the rumen degradation of the roughage resources.

Collaborative Research Program to Investigate the Degradation Mechanisms on paper and Conservation Techniques between Korea and France (종이 손상과 보존 기술에 대한 한·불 교류연구)

  • Chung, Yong-jae;Choi, Jung-eun;Lee, Kyu-shik
    • 보존과학연구
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    • s.28
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    • pp.149-168
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    • 2007
  • This project aims to develop a partnership between France and Korea; the field of paper conservation with two mains objectives. The first object is to study the possibilities of implementing the use of traditional Korean paper in conservation workshops in France and Europe. The second one is to establish a long term collaborative research program to investigate the degradation mechanisms of ancient papers and particularly early waxed manuscripts founded in Korea and France which are now brittle and fallen into parts. This problem deserves an investigation using analytical tools such as SEC/MALS, GC/MS to understand the interactions between wax and cellulose during the ageing process and to recommend proper conservation treatment. A research plan could be establish for both institution in order to facilities an exchange of visiting scholars (scientist, post-doc) to implement and share knowledge and for carrying out the research.

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Effect of Feed Monochloramination on Performance of RO Membranes (NH2Cl 사용으로 인한 RO 막의 성능 향상)

  • Hong, Seungkwan;Reiss, Robert;Taylor, James S.;Beverly, Sharon
    • Journal of Korean Society of Water and Wastewater
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    • v.19 no.3
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    • pp.312-317
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    • 2005
  • The 15 month pilot study was performed to investigate the effect of pretreatment by monochloramine ($NH_2Cl$) on the performance of RO membranes made of cellulose acetate (CA) and polyamide (PA). Both RO membranes experienced severe biological fouling without any pretreatment during the treatment of highly organic surface water in Florida, USA. Feed monochloramination at 5 mg/L significantly minimized productivity loss by effective control of biofouling. The CA membrane did not show any structural damages by monochloramine, while the PA membrane suffered from a gradual loss of membrane integrity by chlorine oxidation, which was characterized as an increase in productivity and a decrease in selectivity. The degradation of PA membrane increased with increasing monochloramine dose.