• Parasites, Hosts and Diseases
• /
• v.50 no.4
• /
• pp.361-364
• /
• 2012

The mature cyst of Acanthamoeba is highly resistant to various antibiotics and therapeutic agents. Cyst wall of Acanthamoeba are composed of cellulose, acid-resistant proteins, lipids, and unidentified materials. Because cellulose is one of the primary components of the inner cyst wall, cellulose synthesis is essential to the process of cyst formation in Acanthamoeba. In this study, we hypothesized the key and short-step process in synthesis of cellulose from glycogen in encysting Acanthamoeba castellanii, and confirmed it by comparing the expression pattern of enzymes involving glycogenolysis and cellulose synthesis. The genes of 3 enzymes, glycogen phosphorylase, UDP-glucose pyrophosphorylase, and cellulose synthase, which are involved in the cellulose synthesis, were expressed high at the 1st and 2nd day of encystation. However, the phosphoglucomutase that facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate expressed low during encystation. This report identified the short-cut pathway of cellulose synthesis required for construction of the cyst wall during the encystation process in Acanthamoeba. This study provides important information to understand cyst wall formation in encysting Acanthamoeba.

• Textile Coloration and Finishing
• /
• v.5 no.3
• /
• pp.221-228
• /
• 1993

Cellulose acetate and methyl cellulose were synthesized from waste cellulose in order to make waste knit on value added highly. Crystal waste cellulose by oxidation using $HIO_4$ and then acetylation was decrystallized. A degee of crystallinity was measured by X-ray diffraction and the structure was identified by FT-IR spetroscopy, respectively. Cellulose acetate was prepared from the reaction of decrystallized cellulose with acetic acid, cone-$H_{2}SO_{4}$ and acetic anhydride. Also, structure identification by FT-IR and a degree of crystallinity by X-ray diffraction were performed. DS of the synthesized cellulose acetate was 2.8 and viscosity average molecular weight was 238,000. Also, methyl cellulose was synthesized by treating cellulose acetate with NaOH and iodomethane. DS of the synthesized methyl cellulose was 3.0. Glucose unit with three hydroxy groups was all substituted by methoxyl groups. It was identified by FT-IR spectroscopy. Also, the thermal properties of the synthesized methyl cellulose were examined by DSC. It shewed two shewed melting peaks at 22$0^{\circ}C$ and 24$0^{\circ}C$ in the 2nd scan. It proved that DS=3.0 of methyl cellulose was a thermotropic liquid crystal.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
• /
• v.16 no.1
• /
• pp.19-25
• /
• 2010

It is becoming increasingly important to utilize alternative raw materials for plastic industries other than petrochemical-based plastics. Cellulose derivatives were the bases of the original synthetic plastics. Cellulose and its derivatives, especially the widely used cellulose acetate (CA), constitute one of the main classes of raw materials for production on artificial fibers, films, plastics etc. CA is one of the well known polymers produced from naturally available plant substance. Many researches have focused on the isolation of cellulose from the plant cells to use them as cellulose derivatives and composite materials. Chemical and mechanical treatments provide changes on the molecular structures influencing the fundamental properties of these naturally abundant polymer. The aim of this review article is to review biodegradation, synthesis, formation, and utilization of cellulose esters, especially di-acetate, used in packaging related researches.

• Journal of Plant Biology
• /
• v.29 no.4
• /
• pp.263-284
• /
• 1986

Effects of putrescine, spermidine and spermine on the activity of D-glucose-6-phosphate cyclohydrolase in the Daucus carota L. protoplast cultured for 4 days and effects of polyamines on the incorporation of D-[u-14C]-glucose treated to protoplasts in culture-medium were investigated. The activity of D-glucose-6-phosphate cyclohydrolase was increased by polyamines and among them spermine was the most effective. Polyamiens increased protein synthesis and this due to the increasing effect of the polyamines on the synthesis of glycoprotein which is one of cell wall components. The synthesis of cell polysaccharides, such sa pectic substances, hemicelluloses and cellulose was increased by polyamines, which stimulated synthesis of pectin substances, and hemicellulose more greatly than that of cellulose, and spermidine was the most effective. In the light of the above results it seems that the polyamines increase cell wall regeneration by the stimulation of enzyme activities which synthesize cell wall components.

• Analytical Science and Technology
• /
• v.10 no.4
• /
• pp.291-299
• /
• 1997

2,2'-iminodibenzoic acid-cellulose was prepared by reacting 2,2'-iminodibenzoic acid salt with cellulose-Cl obtained by chlorination of cellulose-OH which is the major component of sawdust. The adsorptivity of Pb(II) and Cu(II) was studied using the synthetic chelating adsorbent. The adsorption amounts of those ions increased with increasing pH and the optimum adsorption time of metal ion was about 1hr. The adsorptivity of Pb(II) was larger than that of Cu(II).

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
• /
• 2011.10a
• /
• pp.133-141
• /
• 2011

• Journal of the Korean Wood Science and Technology
• /
• v.52 no.5
• /
• pp.504-523
• /
• 2024

Timber industry waste is being examined for cellulose manufacturing to give important benefits. The study's goal is to investigate the properties of cellulose powder derived from sawdust waste after it has been reinforced with ferrous-ferric oxide nanoparticles (Fe₃O₄-NPs). Sawdust cellulose powder was produced from Sengon (Albizia chinensis) wood waste in this study. The crushed sawdust waste is handled with alkalization and bleaching. Cellulose powder is then reinforced with Fe₃O₄-NPs at 10 wt.%, 20 wt.%, and 30 wt.%. The magnetic cellulose powder was analysed by X-ray diffraction, Fourier Transform Infrared, scanning electron microscopy morphology, magnetic vibrating sample magnetometer, Brunauer-Emmett teller, and adsorption tests for Methylene Blue and Congo Red dyes. Structure study identifies sawdust as cellulose 1β, with peaks at 14°, 16°, and 22° diffraction angles. The addition of Fe₃O₄-NPs reduces the crystalline index of sawdust cellulose powder from 68.50% to 63.38%, and functional group bond analysis revealed many peak shifts indicating a change in the chemical bonds of magnetic cellulose powder. Incorporating Fe₃O₄-NPs into sawdust cellulose powder confers magnetic and superparamagnetic properties to the sawdust cellulose. Similarly, the surface texture of magnetic cellulose seems rougher as the surface area increases. These parameters imply a 31.8% increase in Congo Red adsorption, using adsorption kinetics based on the pseudo-first-order model.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2014.02a
• /
• pp.315.1-315.1
• /
• 2014

The use of cellulose papers has recently attracted much attention in various device applications owing to their natural advantageous properties of earth's abundance, bio-friendly, large-scale production, and flexibility. Conventional metal oxides with novel structures of nanorods, nanospindles, nanowires and nanobelts are being developed for emerging electronic and chemical sensing applications. In this work, both ZnO (n-type) nanorod arrays (NRAs) and CuO (p-type) nanospindles (NSs) were synthesized on cellulose papers and the p-n junction property was investigated using the electrode of indium tin oxide coated polyethylene terephthalate film. To synthesize ZnO and CuO nanostructures on cellulose paper, a simple and facile hydrothermal method was utilized. First, the CuO NSs were synthesized on cellulose paper by a simple soaking process, yielding the well adhered CuO NSs on cellulose paper. After that, the ZnO NRAs were grown on CuO NSs/cellulose paper via a facile hydrothermal route. The as-grown ZnO/CuO NSs on cellulose paper exhibited good crystalline and optical properties. The fabricated p-n junction device showed the I-V characteristics with a rectifying behaviour.

• Journal of Microbiology and Biotechnology
• /
• v.9 no.6
• /
• pp.704-708
• /
• 1999

In addition to catalyzing the synthesis of glucan from sucrose as a primary reaction, glucansucrase also catalyzes the transfer of glucose from sucrose to other carbohydrates that are present or are added to the reaction digest. Using dextransucrase and altemansucrase, prepared from Leuconostoc mesenteroides B-742CBM and B-1355C, respectively, we modified the bacterial cellulose in Acetobacter xylinum ATCC10821 culture, and then produced a characteristic cellulose that is soluble and has a new structure. There were also some partially modified insoluble cellulose and oligosaccharides in the modification culture. After methylation and following acid hydrolysis of both the soluble and insoluble glucans, there were ($1{\rightarrow}4$) as well as ($1{\rightarrow}6$) and ($1{\rightarrow}3$) glycosidic linkages in the soluble glucan.

• Horticultural Science & Technology
• /
• v.28 no.6
• /
• pp.1039-1050
• /
• 2010

Carrot is one of the useful crops used abundantly in cooking in Western as well as Asia regions such as China and Korea. However, seed coats have hairs which should be removed to increase germination rate. Furthermore, because of seed hairs, farmers face several additional losses, such as time consumption, manpower, capital and so on, for seed handling. To prevent these problems, study of gene related hair formation using short-hair seed lines is required. We analyzed genes related to hair formation from seed through expressed sequenced tag (EST) profiling, based on the fact that the development of carrot seed hair is related to cellulose synthesis pathway in secondary cell wall synthesis stage. To study the gene expression related to hair formation of the carrot seed, a cDNA library was constructed by using the early maturation stage of the short-hair line (659-1) and hairy seed line (677-14). In short-hair (659-1) and hairy seed (677-14) lines, results from of EST profiling through BLASTX search analysis using the NCBI database showed that 172 and 224 unigenes had significant homology with known protein sequences, whereas 233 and 192 unigenes were not, respectively. All ESTs were grouped into 16 categories according to their putative functions. Twenty nine unigenes among all ESTs were considered to be genes regulating seed hair development from cellulose synthesis pathway during secondary cell wall synthesis stage; in results, 14 unigenes related to seed hair development were found only in hairy seed line.