• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1604-1609
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• 2008

A series of in vitro and in vivo experiments were conducted to investigate the effects of the mixture of Tween 80 and cellulolytic enzymes (xylanase and cellulase) on total tract nutrient digestibility and rumen cellulolytic bacterial adhesion rates in Holstein steers. Ground timothy hay sprayed with various levels of Tween 80 and cellulolytic enzymes was used as substrates in an in vitro experiment to find out the best combinations for DM degradation. The application level of 2.5% (v/w) Tween 80 and the combination of 5 U xylanase and 2.5 U cellulase per gram of ground timothy hay (DM basis) resulted in the highest in vitro dry matter degradation rate (p<0.05). Feeding the same timothy hay to Holstein steers also improved in vivo nutrient (DM, CP, CF, NDF and ADF) digesibilities compared to non-treated hay (p<0.05). Moreover, Tween 80 and enzyme combination treatment increased total ruminal VFA and concentrations of propionic acid and isovaleric acid with decreased acetate to propionate ratio (p<0.001). However, adhesion rates of Fibrobacter succinogenes and Ruminococcus flavefaciens determined by Real Time PCR were not influenced by the treatment while that of Ruminococcus albus was decreased (p<0.05). The present results indicate that a mixture of Tween 80 and cellulolytic enzymes can improve rumen environment and feed digestibility with variable influence on cellulolytic bacterial adhesion on feed.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.23.1-23.1
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• 2008

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.2
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• pp.451-454
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• 2006

This paper presents an overview of ONP deinking efficiency with cellulolytic enzymes and synthetic collector in alkaline pH. Deinking is a series of unit operations designed to detach ink from cellulose fibers and separate the dispersed ink from the pulp slurry. Deinking chemicals are process aids that enable expensive mill equipment used in these unit operations to operate more efficiently - often much more efficiently. We propose the blended deinking agent with cellulolutic enzymes and synthetic collector in deinking pulp of conventional alkaline method. The deinking efficiency of old news print in alkaline pH was enhanced with enzyme treatments. The brightness of deinked pulp was increased with less residual ink particles and yield of enzymatic deinked pulp was improved compared to the deinked pulp of conventional alkaline method.

• Journal of Microbiology and Biotechnology
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• 제31권5호
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• pp.740-746
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• 2021

Efficient cellulolytic enzyme production is important for the development of lignocellulose-degrading enzyme mixtures. However, purification of cellulases from their native hosts is time- and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.449-452
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• 2002

Trichoderma sp. FJ1의 cellulase 생산을 위한 배지조성의 검토에서, 질소원으로서 0.1% bacto peptone을 사용하였을 때 생산성이 향상되었으며, 순수 상업용기질인 avicel과 섬유소 폐기물인 볏짚의 혼합배양에서 높은 효소생산성이 얻어졌다. 경제적인 효소생산성을 위한 기질로 섬유소 폐기물의 농도와 혼합비를 검토한 결과, 1%농도에서 볏짚과 펄프를 50:50으로 사용했을시 CMCase, xylanase, ${\beta}-glucosidase$, avicelase는 24.3, 38.7, 1.5, 0.6 U/ml가 얻어졌다. 이러한 효소생산성은 타 보고서의 결과보다 동등이상의 우위를 보여주고 있으며 섬유소 폐기물의 생물학적 당화기술에 크게 기여하리라 사료된다.

• Current Research on Agriculture and Life Sciences
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• 제22권
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• pp.49-56
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• 2004

Cellulolytic enzymes were prepared from alkaline resistant microorganisms which were newly screened from calcic soil. Characteristics of enzymes and enzymatic deinking efficiency of wastepaper were investigated. The results were summarized as fellows: 1. The recovery rate of crude enzyme was 93.7% in Bio-B and 57.4% in Bio-F. 2. The protein content in crude enzymes was lowest and the thermal stability of crude enzymes was highest in Bio-F. 3. The brightness gain of Bio-F deinked pulp was best in ONP and Bio-B deinked pulp was best in MOW. 4. The reject yield was increased with enzymatic deinking flotation process. 5. The residual ink area of paper was increased with enzymatic deinking and large size of ink particles were remained in paper.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.1
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• pp.123-128
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• 2006

Enzymatic deinking method can avoids the alkaline environment as usual required in chemical deinking, which consequently cuts chemical costs and reduced the white water pollution. The electrostatic wastepaper was dinked with commercial cellulolytic enzymes and surfactant in neutral pH and the effectiveness of deinking and the physical properties of deinked pulp were evaluated. The disintegrating efficiency of the electrostatic wastepaper in neutral pH was enhanced with enzyme treatments. Although the freeness of deinked pulp with enzymes was higher than that of deinked pulp with chemical deinking agents, the brightness of the enzymatic deinked pulp was slightly lower than that of the chemical deinked pulp. But, by additions of nonionic surfactants, the brightness of deinked pulp was increased with less residual ink particles and mechanical properties of enzymatic deinked pulp was improved compared to the deinked pulp of conventional alkaline method.

• 펄프종이기술
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• 제39권5호
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• pp.12-19
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• 2007

Enzymatic deinking method can avoids the alkaline environment as usual required in chemical deinking, which consequently cuts chemical costs and reduced the white water pollution. The electrostatic wastepaper was dinked with commercial cellulolytic enzymes and surfactant in neutral pH and the effectiveness of deinking and the physical properties of deinked pulp were evaluated. The disintegrating efficiency of the electrostatic wastepaper in neutral pH was enhanced with enzyme treatments. Although the freeness of deinked pulp with enzymes was higher than that of deinked pulp with chemical de inking agents, the brightness of the enzymatic deinked pulp was slightly lower than that of the chemical deinked pulp. But, by additions of nonionic surfactants, the brightness of deinked pulp was increased with less residual ink particles and mechanical properties of enzymatic deinked pulp was improved compared to the deinked pulp of conventional alkaline method.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.340-345
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• 1999

A novel simultaneous saccharification and fermentation (SSF) process from the microcrystalline cellulose to ethanol was developed by using $\delta$-integrated recombinant cellulolytic Saccharomyces cerevisiae L2612$L2612\deltaGC$, which can utilize cellulose as carbon and energy sources. The optimum amount of enzymes needed for the efficient conversion of cellulose to ethanol at $30^{\circ}C$ was determined with commercial cellulolytic enzymes. By fed-batch cultivation, the heterologous cellulolytic enzymes were accumulated up to 42.67% of the total cellulase and 29% of the $\beta$-glucosidase needed for the efficient SSF process. When this $\delta$-integrated recombinant yeast was applied to the successive SSF step for ethanol production, 20.35 g/l of ethanol was produced after 12 h from 50 g/l of microcrystalline cellulose. By using this novel SSF process, a considerable amount of commercial enzymes was reduced.

• 한국균학회지
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• 제27권2호통권89호
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• pp.94-99
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• 1999

섬유소자원을 이용하기 위하여 S. rugosoannulata를 합성배지에서 배양하여 cellulase 생산 최적 배양조건을 검토한 결과는 다음과 같다. S. rugosoannulata에 의한 cellulase 생산은 세 효소 모두 $40^{\circ}C$가 최적온도이었고, Avicelase와 ${\beta}-glucosidase$는 pH 5.0, CMCase는 pH 4.0이 최적조건 이었다. 탄소원은 CMCase와 ${\beta}-glucosidase$는 xylose를 Avicelase는 maltose를 탄소원으로 했을 때 최고의 생산을 나타냈으며, xylose의 최적농도는 CMCase는 1.0%, Avicelase는 0.8%, ${\beta}-glucosidase$는 1.1%이었다. 질소원은 무기질소원으로 $NH_4Cl$ 첨가시 최고의 생산성을 나타냈으며, 최적농도는 CMCase는 0.3을 Avicelase와 ${\beta}-glucosidase$는 0.4%이었으며, 유기질소원으로는 malt extarct 첨가시 높은 생산성을 나타냈으며, 최적농도는 CMCase와 Avicelase는 1.0%, ${\beta}-glucosidase$는 1.3%이었다. 무기염류로는 $CoCl_2$ 첨가시 최고의 생산성을 나타냈으며, 최적농도는 세 효소 모두 0.35%이었다.