Journal of Microbiology and Biotechnology

  • 제9권3호
  • /
  • Pages.340-345
  • /
  • 1999
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Novel SSF Process for Ethanol Production from Microcrystalline Cellulose Using the $\delta$-Integrated Recombinant Yeast, Saccharomyces cerevisiae L2612$\delta$GC

  • Cho, Kwang-Myung (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Institute for Molecular Biology and Genetics) ;
  • Yoo, Young-Je (Interdisciplinary Program for Biohemical Engineering and Biotechnology, Institute for Molecular Biology and Genetics, School of Chemical Engineering, Seoul National University)
  • 발행 : 1999.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A novel simultaneous saccharification and fermentation (SSF) process from the microcrystalline cellulose to ethanol was developed by using $\delta$-integrated recombinant cellulolytic Saccharomyces cerevisiae L2612$L2612\deltaGC$, which can utilize cellulose as carbon and energy sources. The optimum amount of enzymes needed for the efficient conversion of cellulose to ethanol at $30^{\circ}C$ was determined with commercial cellulolytic enzymes. By fed-batch cultivation, the heterologous cellulolytic enzymes were accumulated up to 42.67% of the total cellulase and 29% of the $\beta$-glucosidase needed for the efficient SSF process. When this $\delta$-integrated recombinant yeast was applied to the successive SSF step for ethanol production, 20.35 g/l of ethanol was produced after 12 h from 50 g/l of microcrystalline cellulose. By using this novel SSF process, a considerable amount of commercial enzymes was reduced.

키워드

참고문헌

  1. Yeast v.11 Induced expression of bacterial β-glucosidase activity in Saccharomyces Adam, A. C.;M. Rubio-Texeira;J. Polaina
  2. Yeast v.5 Secretion and glycosylation of Clostridium thermocellum endoglucanase A encoded by the celA gene in Saccharomyces cerevisiae Benitez, J.;A. Silva;R. Vazquez;M. D. Noa;C. P. Hollenberg
  3. Appl. Biochem. Biotechnol. v.28/29 Selection of thermotolerant yeasts for simultaneous saccharification and fermentation of cellulose to ethanol Ballesteros, I.;M. Ballesteros;A. Cabanas;J. Carrasco;C. Martin;M.J. Negro;F. Saez;R. Saez
  4. Biotechnol. Lett. v.19 Simultaneous saccharification and fermentation of straw to ethanol using thermotolerant yeast strain Kluyveromyces marxianus imb3 Boyle, M.;N. Barron;A. P. McHale
  5. Enzyme Microb. Technol. v.25 δ-Integration of endo/exo-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol Cho, K.M.;Y.J. Yoo;H.S. Kang
  6. Enzyme Microb. Technol. v.11 Direct fermentation of cellulose to ethanol by Fusarium oxysporum Christakopoulos, P.;B. J. Macris;D. Kekos
  7. Biotechnol. Bioeng. v.44 Saccharification and fermentation of sugar cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway Doran, J. B.;H. C. Aldrich;L. O. Ingram
  8. Enzyme Microb. Technol. v.17 Simultaneous saccharification and fermentation of steam-pretreated willow Eklund,R.;G, Zacchi
  9. US Patent No.3,990,994 Manufacture of alcohol from cellulosic materials using plural ferments Gaus, W. F.;S. Suzuki;M. Takagi
  10. Bioconversion of Forest and Agricultural Residues Simultaneous saccharification and fermentation of cellulosic substrates to ethanol Grohmann, K.;J. N. Sadler (ed.)
  11. J. Ferment. Technol. v.66 Ethanol production in siumltaneous saccharification and fermentation of cellulose with temperature profiling Huang, S. Y.;J. C. Chen
  12. J. Microbiol. Biotechnol. v.8 Invertase production by fed-batch fermentations of recombinant Saccharomyces cerevisiae Koo, J. H.;S. Y. Kim;Y. C. Park;N. S. Han;J. Seo
  13. Appl. Environ. Microbiol. v.63 Cloning of cellobiose phosphoenolpyruvate dependent phosphotransferase gene:Functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides Lai, X.;F.C. Davis;R.B. Hespell;L.O. Ingram
  14. Appl. Environ. Microbiol. v.55 Fermentation of cellulosic substrates in batch and continuous culture by Clostridium thermocellum Lynd, L. R.;H. E. Grethlein;R. H. Wolkin
  15. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller, G. L.
  16. Biotechnol. Bioeng. v.49 Simultaneous sacchrification and extractive fermentation of cellulosic substrates Moritz, J. W.;S. J. B. Duff
  17. J. Microbiol. Biotechnol. v.8 Development of cellobiose-utilizing recombinant yeast for ethanol production from cellulose bydrolyzate Pack, S. P.;K. M. Cho;H. S. Kang;Y. J. Yoo
  18. Appl. Biochem. Biotechnol. v.51/52 Limiting factors in the simultaneous saccharification and fermentation process for conversion of cellulosic biomass to fuel ethanol Philippidis, G. P.;T. K. Smith
  19. Biotechnol. Bioeng. v.41 Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process Philippidis, G. P.;T. K. Smith;C. E. Wyman
  20. Appl. Biochem. Biotechnol. v.17 Themotolerant yeasts for simultaneous saccharification and fermentation of cellulose to ethanol Spindler, D.D.;C.E. Wyman;A. Mohagheghi;K. Grohmann
  21. Biotechnol. Bioeng. v.34 Evaluation of thermotolerant yeast in controlled simultaneous saccharification and fermentations of cellulose to ethanol Spindler, D. D.;C. E. Wyman;K. Grohmann
  22. Appl. Biochem. Biotechnol. v.28/29 The simultaneous saccharification and fermentation of pretreated woody crops to ethanol Spindler, D.D.;C.E. Wyman;K. Grohmann
  23. Enzyme Microb. Technol. v.17 Direct conversion of cellulosic material to ethanol by the intergenic fusant Trichoderma reesei QM9414/Saccharomyces cerevisiae NCIM 3288 Srinivas, D.;K. J. Rao;K. Theodora;T. Panda
  24. Proceedings Bioconversion Symposium A method for production of alcohol directly from cellulose using cellulase and yeast Takagi, M.;S. Abe;S. Suzuki;G. H. Emert;N. Yata
  25. Bioresour. Technol. v.51 A techno-economical comparison of three processes for the production of ethanol from pine von Sivers, M.;G. Zacchi
  26. Biotechnol. Bioeng. v.49 G418 selection and stability of cloned genes integrated at the chromosomal δ-sequences of Saccharomyces cerevisiae Wang, X.;Z. Wang;N. A. Da Silva
  27. Appl. Environ. Microbiol. v.58 Ethanol production from cellobiose, amorphous cellulose and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum Wood, B. E.;L. O. Ingram
  28. Methods Enzymol. v.160 Methods for measuring cellulase activities Wood, T.M.;K.M. Bhat
  29. Bioresour. Technol. v.50 Ethanol production from lignocellulosic biomass:Technology, economics, and opportunities Wyman, C.E.