• Molecular & Cellular Toxicology
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• 제4권2호
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• pp.132-137
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• 2008

Differential gene expression profiling was carried out in the hepatic tissue of medaka fish, Oryzias latipes, after exposure to an organophosphorus pesticide (OPP), Iprobenfos (IBP), a widely used pesticide in agri- and fish-culture, using a medaka cDNA micro array. Twenty six kinds of differentially expressed candidate genes, with 15 and 11 induced and repressed in their gene expressions, respectively, were associated with cytoskeleton (3.8%), development (7.7%), immune (7.7%), metabolism (30.8%), nucleic acid/protein binding (42.3%) and reproduction (7.7%). Of these genes, changes at the transcription level of five were re-evaluated by real-time quantitative PCR (qRT-PCR). Considering the known function of authentic genes, the effects of IBP on the biological activity and pathological aspects in medaka fish were discussed. The identified genes could be used as molecular biomarkers for biological responses to OPPs contamination in an aquatic environment.

• BMB Reports
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• 제32권5호
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• pp.456-460
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• 1999

The promoter of the HSV-1 VP16 gene contains binding sites for the cellular transcription factors such as USF, CTF, and Sp1, each of which affects basal level expression of the VP16 gene. Transcription of the VP16 gene was induced by viral immediate-early proteins, ICP0 and ICP4, in a synergistic manner but repressed by ICP22. To gain further insight into the role of ICP0 in the expression of the VP16 gene during virus infection, several mutants with deletions in each of their transcriptional regulatory elements were generated. According to transient gene expression assays of these mutants using the CAT gene as a reporter, the USF and CTF binding sites were necessary for efficient induction of the promoter in the presence of transfected ICP0 or during virus infection, whereas the Sp1 binding site had little effect on ICP0-mediated VP16 expression. These results indicate that the immediate early proteins of HSV-1 regulate expression of the VP16 gene during virus infection by modulating the activities of cellular transcription factors such as USF and CTF.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1222-1228
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• 2006

Two kinds of Haematococcus pluvialis cells (green vegetative cells cultivated under optimal cell culture conditions and red cyst cells maintained under high light stress conditions to induce astaxanthin production) were used to investigate the protein expression profiles by two-dimensional electrophoresis, image analysis, and peptide mass fingerprinting. The cellular accumulation of astaxanthin was evident after exposure to high light intensity and reached the maximum cellular level after 78 h of high light stress. In a 2-D electrophoresis analysis, 22 proteins were upregulated over 2-fold in the red cyst cells when compared with the green vegetative cells and selected for further analysis by chemically assisted fragmentation (CAF)-MALDI-TOF sequencing to identify the protein functions. Among 22 different spots, several key enzymes specific to the carotenoid pathway, including isopentenyl pyrophosphate isomerase (IPP) and lycopene $\beta$-cyclase, appeared in H. pluvialis after exposure to high light intensity. Therefore, IPP and lycopene $\beta$-cyclase would appear to be involved with carotenoid accumulation in the cytoplasm, as these peptides were preferentially upregulated by high light intensity preceding an increase in carotenoid, and only these forms were detected in the red cyst cells.

• 여성건강간호학회지
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• 제10권2호
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• pp.144-149
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• 2004

Purpose: The purpose of this study was to evaluate whether the effect of Intenet diabetes education varied by gender. Method: An experimental group assessed pre- and post intervention was used to assess the effectiveness of diabetes education by nurses. Forty patients separated into two groups by gender partook in the study. The goal of the intervention was to keep blood glucose concentrations close to normal range. The intervention was applied weekly for 3 months. Participants were requested to input their blood glucose level, diet, and exercise diary everyday at http://www.biodang.com by cellular phone or wire Internet. The researcher sent optimal recommendations to each patient using the short message service of cellular phones and wire Internet. All medication adjustments were communicated to the subjects' doctors. The plasma glucose levels, serum lipids, and care satisfaction were measured before and after the intervention. Result: Glycosylated hemoglobin (HbA1c) decreased 1.6% and 0.8% in male and female patients respectively after 3 months of education. Total cholesterol decreased 37.2mg/dl in male patients but increased 80.5mg/dl in female patients. Conclusion: These findings indicated that Internet diabetes education could improve HbA1c and total cholesterol in male patients.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1198-1203
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• 2007

Conidial adhesion and appressorium formation of Magnaporthe oryzae on the rice surface are important early events in the infection process. As an initiative step to understand the mechanisms underlying these cellular processes at a biochemical level, the effect of a human fibronectin antibody (HFA) and RGD peptides on conidial adhesion and appressorium formation was evaluated. HFA inhibited conidial adhesion and appressorium formation in a dosage-dependent manner. RGD peptides also inhibited these cellular events. Conidial adhesion and appressorium formation inhibited by RGD peptides were restored by chemicals involved in the cyclic AMP-dependent signaling pathway. These results suggest that extracellular matrix proteins might be involved in conidial adhesion and appressorium formation through integrin-like receptor mediation and modulation of cAMP-dependent signaling in the cells.

• International Journal of Oral Biology
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• 제37권4호
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• pp.175-180
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• 2012

Skin-derived precursor cells (SKPs) are multipotent, sphere-forming and embryonic neural crest-related precursor cells that can be isolated from dermis. It is known that the properties of porcine SKPs can be enhanced by leukemia inhibitory factor (LIF) which is an essential factor for the generation of embryonic stem cells in mice. In our present study, to enhance or maintain the properties of murine SKPs, LIF was added to the culture medium. SKPs were treated with 1,000 IU LIF for 72 hours after passage 3. Quantitative real time RT-PCR was then performed to quantify the expression of the pluripotent stem cell specific genes Oct4, Nanog, Klf4 and c-Myc, and the neural crest specific genes Snai2 and Ngfr. The results show that the expression of Oct4 is increased in murine SKPs by LIF treatment whereas the level of Ngfr is decreased under these conditions. Interestingly, LIF treatment reduced Nanog expression which is also important for cell proliferation in adult stem cells and for osteogenic induction in mesenchymal stem cells. These findings implicate LIF in the maintenance of stemness in SKPs through the suppression of lineage differentiation and in part through the control of cell proliferation.

• 한국통신학회논문지
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• 제27권1B호
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• pp.13-23
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• 2002

이동 호스트는 무선 통신망의 낮은 대역폭과 호스트들의 이동성, 부족한 저장장치와 배터리 수명 등으로 인하여 결함 발생 가능성이 높다. 이동 호스트의 결함에 효율적으로 대처하기 위한 결함 허용 기법에 관한 연구가 많이 진행되어 왔다. 셀룰라 네트워크에서는 이동 호스트이외에도 이동 호스트를 연결시키는 기지국은 보다 높은 수준의 가용도를 요구하므로 기지국의 결함에 대한 연구도 필수적이다. 본 논문에서는 이동 호스트 결함 복구를 위한 체크포인팅 기법을 기반으로 기지국 결함 복구를 위한 체크포인팅 이중화 기법을 제안하고 성능을 분석하였다. 또한 이동 호스트의 결함 복구를 위해 체크포인트가 존재하는 기지국의 복구를 기다리는 방법과 다른 기지국의 체크포인트를 이용하는 방법의 성능을 비교 분석하였다.

• Molecules and Cells
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• 제40권12호
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• pp.897-905
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• 2017

Cellular protein homeostasis is maintained by two major degradation pathways, namely the ubiquitin-proteasome system (UPS) and autophagy. Until recently, the UPS and autophagy were considered to be largely independent systems targeting proteins for degradation in the proteasome and lysosome, respectively. However, the identification of crucial roles of molecular players such as ubiquitin and p62 in both of these pathways as well as the observation that blocking the UPS affects autophagy flux and vice versa has generated interest in studying crosstalk between these pathways. Here, we critically review the current understanding of how the UPS and autophagy execute coordinated protein degradation at the molecular level, and shed light on our recent findings indicating an important role of an autophagy-associated transmembrane protein EI24 as a bridging molecule between the UPS and autophagy that functions by regulating the degradation of several E3 ligases with Really Interesting New Gene (RING)-domains.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회/대한산업공학회 2003년도 춘계공동학술대회
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• pp.441-447
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• 2003

Recently there is a growing Interest In mobile cellula r network providing multimedia service. However, the link bandwidth of mobile cellular network is not sufficient enough to provide satisfactory services to use rs. To overcome this problem, an adaptive framework has been proposed in this study, we propose a new method of estimating DPR(degradation period ratio) in an adaptive multimedia network where the bandwidth of ongoing call can be dynamically adjusted during its lifetime. DPR is a QoS(quality of service) parameter which represents the ratio of allocated bandwidth below a pre-defined target to the whole service time of a call. We improve estimation method of DPR using DTMC(discrete time Markov chain) model. We also calculate mean degradation period and degradation probability more precisely than in existing studies. Under Threshold CAC(call admission control) algorithm, we present analytically how to guarantee QoS to users and illustrate the method by numerical examples. The proposed method is expected to be used as one of CAC schemes in guaranteeing predefined QoS level of DPR

• 대한의생명과학회지
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• 제24권1호
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• pp.50-54
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• 2018

N-nitroso-N-methylurea (NMU) and N-nitroso-N-ethylurea (NEU), direct alkylating chemical mutagens and carcinogens, are shown to be the upregulators of cellular $NF-{\kappa}B$, regulating various genes that mediate tumorigenesis and carcinogenesis. Nitric oxide (NO), a toxic reactive radical gas, has been known to induce programmed cell death or apoptosis in various cells. Therefore, the assessment of NO production was examined to elucidate the possible contribution of NO release to the chemical carcinogenic potency of NMU and NEU in human skin cells. NMU and NEU did not alter the NO production, but they caused a significant downregulation of the NO generation on lipopolysaccharide (LPS)-induced NO production at concentrations ranging from $2{\sim}5{\mu}M$. The degree of downregulation of NO by NMU and NEU decreased up to 15% and 20%, respectively, compared to the control. These results demonstrate that the LPS-inducible keratinocytes NO synthase is involved in modulating carcinogenic potency by NMU and NEU, and the regulation of the cellular $NF-{\kappa}B$ activity by NMU and NEU is negatively correlated with the level of LPS-induced NO production in human skin cells. The findings of this study suggest the hypothesis that NMU and NEU-induced carcinogenesis may be associated with the downregulation of NO production, and the inducible NO may play an important role in NMU and NEU-induced carcinogenicity in human epidermal keratinocytes.