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Proteomic Analysis of Protein Expression Patterns Associated with Astaxanthin Accumulation by Green Alga Haematococcus pluvialis (Chlorophyceae) Under High Light Stress  

Kim Jeong-Dong (Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University)
Lee Woo-Sung (Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University)
Kim Beob-Min (Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University)
Lee Choul-Gyun (Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.8, 2006 , pp. 1222-1228 More about this Journal
Abstract
Two kinds of Haematococcus pluvialis cells (green vegetative cells cultivated under optimal cell culture conditions and red cyst cells maintained under high light stress conditions to induce astaxanthin production) were used to investigate the protein expression profiles by two-dimensional electrophoresis, image analysis, and peptide mass fingerprinting. The cellular accumulation of astaxanthin was evident after exposure to high light intensity and reached the maximum cellular level after 78 h of high light stress. In a 2-D electrophoresis analysis, 22 proteins were upregulated over 2-fold in the red cyst cells when compared with the green vegetative cells and selected for further analysis by chemically assisted fragmentation (CAF)-MALDI-TOF sequencing to identify the protein functions. Among 22 different spots, several key enzymes specific to the carotenoid pathway, including isopentenyl pyrophosphate isomerase (IPP) and lycopene $\beta$-cyclase, appeared in H. pluvialis after exposure to high light intensity. Therefore, IPP and lycopene $\beta$-cyclase would appear to be involved with carotenoid accumulation in the cytoplasm, as these peptides were preferentially upregulated by high light intensity preceding an increase in carotenoid, and only these forms were detected in the red cyst cells.
Keywords
Astaxanthin; high light intensity; isopentenyl pyrophosphate isomerase; lycopene ${\beta}$-cyclase;
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Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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1 Sun, Z., F. X. Cunningham, and E. Gantt. 1998. Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte. Proc. Natl. Acad. Sci. USA 95: 11482-11488
2 Yabe, N., T. Takahashi, and Y. Komeda. 1994. Analysis of tissue-specific expression of Arabidopsis thaliana Hsp9O-family gene Hsp81. Plant Cell Physiol. 35: 1207-1219   DOI
3 Young, A. J. and G. Britton. 1993. Carotenoids in pigmentprotein complexes, pp. 72-95. In: Carotenoids in Photosynthesis. Chapman and Hall, London
4 Zlotnik, I., A. Sukenik, and Z. Dubinsky. 1993. Physiological and photosynthetic changes during the formation of red aplanospores in the Chlorophyte Haematococcus pluvialis. J. Phycol. 29: 463-469   DOI   ScienceOn
5 Carpita, N., M. Tierney, and M. Campbell. 2001. Molecular biology of the plant cell wall: Searching for the genes that define structure, architecture and dynamics. Plant Mol. BioI. 47: 1-5   DOI   ScienceOn
6 Dumford, D. G., J. A. Deane, S. Tan, G. I. McFadden, E. Gantt, and B. R. Green. 1999. A phylogenetic assessment of the eukaryotic light-harvesting antenna proteins, with implications for plastid evolution. J. Mol. Evol. 48: 59-68   DOI
7 Kobayashi, M., T. Kakizono, and S. Nagai. 1993. Enhanced carotenoid biosynthesis by oxidative stress in acetate-induced cyst cells of a green unicellular alga, Haematococcus pluvialis. Appl. Environ. Microbiol. 59: 867-873
8 Perrin, R., C. Wilkerson, and K. Keegstra. 2001. Golgi enzymes that synthesize plant cell wall polysaccharides: Finding and evaluating candidates in the genomic era. Plant Mol. Biol. 47: 115-130   DOI   ScienceOn
9 Rhee, S. J., C.-Y. J. Lee, M.-R. Kim, and C.-H. Lee. 2004. Potential antioxidant peptides in rice wine. J. Microbiol. Biotechnol. 14: 715 - 721   과학기술학회마을
10 Ritchie, R. J. 1998. Bioenergetics of membrane transport in Synechococcus R-2 (Anacystis nidulans, S. leopoliensis) PCC7942. Can. J. Bot. 76: 1127-1145   DOI
11 Suh, I. S. and S. B. Lee. 2001. Cultivation of a cyanobacterium in an internally radiating air-lift photobioreactor. J. Appl. Phycol. 13: 381-388   DOI   ScienceOn
12 Altendorf, K., A. Siebers, and W. Epstein. 1992. The Kdp ATPase of Escherichia coli. Ann. N. Y. Acad. Sci. 671: 228-243   DOI
13 Kim, J.-H., S.-W Kim, C.-W Kim, and H.-I. Chang. 2005. Therapeutic effect of astaxanthin isolated from Xanthophyllomyces dendorhous mutant against naproxeninduced gastric antral ulceration in rats. J. Microbiol. Biotehnol. 15: 633-639   과학기술학회마을
14 Heide, H., H. M. Kalisz, and H. Follmann. 2004. The oxygen evolving enhancer protein I (OEE) of photosystem II in green algae exhibits thioredoxin activity. J. Plant Physiol. 161: 139-149   DOI   ScienceOn
15 Teramoto, H., T. Ono, and J. Minagawa. 2001. Identification of Lhcb gene family encoding the light-harvesting chlorophyllalb proteins of photosystem II in Chlamydomonas reinhardtii. Plant Cell Physiol. 42: 849-856   DOI   ScienceOn
16 Cunningham, F. X., B. Pogson, Z. R. Sun, K. A McDonald, D. DellaPenna, and E. Gantt. 1996. Functional analysis ofthe beta and epsilon Iycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8: 1613 -1626   DOI   ScienceOn
17 Huang, F., I. Parmryd, F. Nilsson, A. L. Persson, H. B. Pakrasi, B. Andersson, and B. Norling. 2002. Proteomics of Synechocystis sp. strain PCC 6803. Identification of plasma membrane proteins. Mol. Cell. Proteomics 1: 956-966   DOI
18 Gassel, M. and K. Altendorf. 2001. Analysis of KdpC of the $K^+$-transporting KdpFABC complex of Escherichia coli. Eur. J. Biochem. 268: 1772-1781   DOI   ScienceOn
19 Tokutsu, R., H. Teramoto, Y. Takahashi, T. A. Ono, and J. Minagawa. 2004. The light-harvesting complex of photo system I in Chlamydomonas reinhardtii: Protein composition, gene structures and phylogenie implications. Plant Cell Physiol. 45: 138-145   DOI   ScienceOn
20 Cunningham, F. X., Z. R. Sun, D. Chamovitz, J. Hirschberg, and E. Gantt. 1994. Molecular-structure and enzymatic function of Iycopene cyclase from the cyanobacterium Synechococcus sp. strain PCC7942. Plant Cell 6: 1107-1121   DOI   ScienceOn
21 Choi, S.-L., I. S. Suh, and C.-G Lee. 2003. Lumostatic operation of bubble column photobioreactors for Haematococcus pluvialis cultures using a specific light uptake rate as a control parameter. Enzyme Microb. Technol. 33: 403-409   DOI   ScienceOn
22 Cassab, G. I. 1998. Plant cell wall proteins. Annu. Rev. Plant Physiol. Plant Mol. BioI. 49: 281-309   DOI
23 Wang, S. B., Q. Hu, M. Sommerfeld, and F. Chen. 2004. Cell wall proteomics of the green alga Haematococcus pluvialis (Chlorophyceae). Proteomics 4: 692-708   DOI   ScienceOn
24 Cunningham, F. X. and E. Gantt. 1998. Genes and enzymes of carotenoid biosynthesis in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49: 557 -583   DOI
25 Sanchez-Fernandez, R., T. G. E. Davies, J. O. D. Coleman, and P. A. Rea. 2001. The Arabidopsis thaliana ABC protein superfamily, a complete inventory. J. Biol. Chem. 276: 30231-30244   DOI   ScienceOn
26 Waffenschmidt, S. J., P. Woessner, K. Beer, and U. W. Goodenough. 1993. Isodityrosine cross-linking mediates insolubilization of cell walls in Chlamydomonas. Plant Cell 5: 809-820   DOI   ScienceOn
27 Weiss, D. and U. Lutz-Meindl. 1999. Heat response in the green alga Micrasterias denticulata (Desmidiaceae): Immunodetection and localization of BiP and heat shock protein 70. Nova Hedwigia 69: 217 -228
28 Takahashi, Y. and U. Tokumoto. 2002. A third bacterial system for the assembly of iron-sulfur clusters with homologs in archaea and plastids. J. Biol. Chern. 277: 28380-28383   DOI   ScienceOn
29 Florencio, J. A, C. R. Soccol, L. F. Furlanetto, T. M. B. Bonfim, N. Krieger, M. Baron, and J. D. Fontana. 1998. A factorial approach for a sugarcane juice-based low cost culture medium: Increasing the astaxanthin production by the red yeast Phaffia rhodozyma. Bioprocess Eng. 19: 161-164
30 Borowitzka, M. A, J. M. Huisman, and A. Osborn. 1991. Culture of the astaxanthin-producing green alga Haematococcus pluvialis. J. Appl. Phycol. 3: 295-304   DOI
31 Reiter, W. D. 2002. Biosynthesis and properties of the plant cell wall. Cur. Opin. Plant Biol. 5: 536-542   DOI   ScienceOn
32 Kang, M.-J., S.-H. Yoon, Y-M. Lee, S.-H. Lee, J.-E. Kim, K.-H. Jung, Y-C. Shin, and S.-W Kim. 2005. Enhancement of Iycopene production in Escherichia coli by optimization of the Iycopene synthetic pathway. J. Microbiol. Biotechnol. 15: 880-886   과학기술학회마을
33 Tjahjono, A. E., Y. Hayama, T. Kakizono, Y. Terada, and N. Nishio. 1994. Hyper-accumulation of astaxanthin in a green-alga Haematococcus pluvialis at elevated-temperatures. Biotechnol. Lett. 16: 133-138   DOI
34 Wang, S. B., F. Chen, M. Sommerfeld, and Q. Hu. 2004. Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae). Planta 220: 17-29   DOI
35 Tan, S., F. X. Cunningham, M. Youmans, B. Grabowski, Z. Sun, and E. Gantt. 1995. Cytochrome f loss in astaxanthinaccumulating red cells of Haematococcus pluvialis (Chlorophyceae): Comparison of photosynthetic activity, photosynthetic enzymes, and thylakoid membrane polypeptides in red and green cells. J. Phycol. 31: 897 -905   DOI
36 Rosenfeld, J., K. Sloan Brown, and A. L. George. 1992. Ingel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis. Anal. Biochem. 203: 173-179   DOI   ScienceOn
37 Eom, H., S. Park, C.-G. Lee, and E. Jin. 2005. Gene expression profiling eukaryotic microalga, Haematococcus pluvialis. J. Microbiol. Biotechnol. 15: 1060-1066   과학기술학회마을
38 Kim, S.-H., J. R. Shinkle, and S. J. Roux. 1989. Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings. Proc. Natl. Acad. Sci. USA 86: 9866-9870
39 Steinbrenner, J. and H. Linden. 2003. Light induction of carotenoid biosynthesis genes in the green alga Haematococcus pluvialis: Regulation by photosynthetic redox control. Plant Mol. Biol. 52: 343-356   DOI   ScienceOn