• KSBB Journal
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• 제15권4호
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• pp.337-341
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• 2000

The decanter is very useful to harvest biomass from plant cell cultures in large-scale process. It is very important to obtain high yield and low moisture content in recovered biomass so as to minimize solvent usage in subsequent extraction steps. Effluent clarity was also affected by the differential speed although this affect was more dramatic at higher flow rates than at lower flow rates. Moisure content was largely unaffected by flow rate. A decrease in moisture content was evident as differential speed decreased.

• KSBB Journal
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• 제13권6호
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• pp.644-648
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• 1998

In the production of a low cost bacterial insecticide, it is important to produce a high spore concentration using low price substrates. Experiments were carried out to investigate the effects of the addition of mineral salts and glucose, and of dissolved oxygen concentration on the cell growth and spore formation of Bacillus thuringiensis var aizawai using a cheap wheat and soybean meal in the batch culture. The maximum viable cell number was 1.2${\times}$109 CFU/mL at 12 hr culture and spore yield was 54.2% at 74 hr culture using an industrial medium containing 20 g/L wheat meal and 30 g/L soybean meal under 1.0 vvm aeration and 200 rpm agitation. The cell growth and the spore formation were not enhanced by the addition of mineral salts in industrial medium, whereas th addition of 10g/L glucose decreased the cell growth and spore formation. We could obtain a maximum viable cell number of 2.2${\times}$109 CFU/mL and spore number of 1.9${\times}$109 CFU/mL at the dissolved oxygen concentration of 60% of saturation. The spore concentration was enhanced approximately by 2 times as compared to the dissolved oxygen concentration of 50%. In the bench-scale culture, the maximum viable cell and spore number were 2.5${\times}$109 CFU/mL, and 2.2${\times}$109 CFU/mL, respectively under 1.0 vvm aeration and 400 rpm agitation. The spore yield was 88% based on the maximum viable cell number. As a result, it was confirmed that the production of high spore concentration could be obtained by a bench-scale culture using an industrial medium.

• 자원환경지질
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• 제47권6호
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• pp.635-643
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• 2014

현재 우리나라의 지하수 개발가능량은 10년빈도 갈수시 함양량으로 결정되며 일정면적 이상의 수문학적 유역 또는 행정구역별로 넓게 구분되어 있어 소규모 유역의 지하수 영향평가에 적용하기가 어려운 면이 있다. 이에 본 연구에서는 셀기반의 지하수 개발가능량을 추정하는 새로운 기법을 경주지역에 대해 제시하였다. 이를 위해 지표수-지하수 통합 수문해석 모형인 SWAT-MODFLOW를 이용하여 셀기반 지하수 함양량을 산정하였다. 지하수 개발가능량을 결정하는 기준은 10년빈도 갈수시 강수량에 함양계수를 곱하여 추정하는 기존방식을 준용했다. 격자별 개발가능량을 산정한 후에는 목적에 따라 경주지역에서 143개로 세분화된 소유역별, 읍면리등 행정구역별로 개발가능량을 제시할 수 있었다. 지하수 이용량과 개발가능량을 연계하여 분석한 결과 경주시 전체 이용율은 평균 24%의 비교적 양호한 이용률을 보였으나 지역적으로는 7.1~108.8%까지 편차가 큰 이용현황을 보이는 것을 쉽게 확인할 수 있었다. 또한 임의의 시군구에서 지역별 추가 개발가능량도 평가할 수 있어 그 활용성이 기대된다.

• 원예과학기술지
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• 제18권1호
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• pp.9-13
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• 2000

플러그묘를 이용하여 불량 기상환경을 회피하며 양파 수급의 안정을 위해 중부지방에서의 양파 생산 가능성을 검토하였다. 정식시기와 육묘일수 실험의 경우 '패총황'을 공시하여 3월 15일과 4월 15일에 162공 트레이에 30, 60, 90일간 육묘후 정식하였으며 적정 육묘 용기 선택은 cell 크기별로 '패총황'을 90일간 육묘해서 3월 15일에 정식하였고 춘파재배시 적품종을 선발코자 '패총황'외 조 중 만생계 6품종을 공시하여 90일간 육묘후 3월 15일에 정식하였다. 플러그묘의 정식시기에 따른 육묘일수는 3월 15일 정식한 60일 묘가 107.2ton/ha로 가장 수량이 많았는데 정식시기에 따라서는 수량에는 유의차가 없고 육묘일수에 따라 차이를 보였다. 육묘용기로는 128공에서 125.0ton/ha로 수량이 가장 많았으며 400공의 경우 미성숙 상태로 수확이 되어 적합치 않은 것으로 사료되며 육묘시 단위 면적당 육묘본수를 고려한다면 200공이나 288공까지도 가능할 것으로 보인다. 품종에 따른 수량은 중생종인 '천주황'이 130.5ton/ha로 가장 많았으나 중부지방의 기후적 여건을 감안할 경우 다소 수량이 적지만 숙기가 빨라 6월 중순 이전에 수확할 수 있는 '용봉황' 등의 조생종 계통이 유리할 것을 사료된다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2004년도 Asia Display / IMID 04
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• pp.895-898
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• 2004

We propose configurations for a transflective in-plane switching (IPS) cell using muti-domain structures. Usually, the cell configurations for a transflective liquid crystal(LC) cell have a complicated structure, because retardation change of transmissive part and reflective part are not same. The transflective LC cell should have two configurations for each part, such as a multi-cell gap structure. With the ion-beam alignment and the horizontal switching LC cell, a simple structure for a transflective LC cell is proposed. The configuration only adopts one cell gap structure, which may help the enhancement of a yield. Their original optical properties in conventional transmissive and reflective type IPS liquid crystal dispaly(LCD) are kept, it shows the wide-viewing angle and the good wavelength dispersion characteristics.

• 한국미생물·생명공학회지
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• 제17권6호
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• pp.624-628
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• 1989

A study on the citric acid production using Saccharomycopsis lipolytica (NRRL Y7576) was carried out in shake-flasks, air-lift and membrane recycle bioreactors. The cells entrapped in Ca-alginate beads were used in shake-flasks and air-lift reactor. Repeated batch fermentation in shake-flasks was successfully performed for 34 days and resulted in a yield of 54%. Increased yield (63%) was obtained in the air-lift reactor operation using nitrogen deficient medium (NDM). In the membrane recycle bioreactor operation, the maximal dry cell mass concentration was 39 g/1 at a dilution rate of 0.02 h$^{-1}$ and the yield with NDM was higher than that with growth medium. In addition, the yield and volumetric productivity with pure oxygen supply were greatly improved compared with those with air supply.

• Applied Science and Convergence Technology
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• 제24권5호
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• pp.196-202
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• 2015

Human blood consists of 55% of plasma and 45% of blood cells such as white blood cell (WBC) and red blood cell (RBC). In plasma, there are many kinds of promising biomarkers, which can be used for the diagnosis of various diseases and biological analysis. For diagnostic tools such as a lab-on-a-chip (LOC), blood plasma separation is a fundamental step for accomplishing a high performance in the detection of a disease. Highly efficient separators can increase the sensitivity and selectivity of biosensors and reduce diagnostic time. In order to achieve a higher yield in blood plasma separation, we propose a novel fluid wing structure that is optimized by COMSOL simulations by varying the fluidic channel width and the angle of the bifurcation. The fluid wing structure is inspired by the inertial particle separator system in helicopters where sand particles are prevented from following the air flow to an engine. The structure is ameliorated in order to satisfy biological and fluidic requirements at the micro scale to achieve high plasma yield and separation efficiency. In this study, we fabricated the fluid wing structure for the efficient microfluidic blood plasma separation. The high plasma yield of 67% is achieved with a channel width of $20{\mu}m$ in the fabricated fluidic chip and the result was not affected by the angle of the bifurcation.

• 펄프종이기술
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• 제34권4호
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• pp.22-29
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• 2002

This research was conducted to investigate the morphological characteristics of fine fibers produced during beating process of high yield pulp treated with ozone and the distribution of lignin in the produced fine fibers. Thermomechanical(TMP) pulp and chemithermomechanical(CTMP) pulp of spruce and CTMP of white birch were beaten to reach 200$m\ell$ CSF, and then the fine fibers were observed using ultraviolet microscope. The fine fibers produced from TMP and CTMP of spruce using treated with ozone for 15 minutes were fragments of fiber surfaces or cell corners, and most of them contained lignin. However, lignin was not observed in the fibers after 15 minutes of ozone treatment. The fine fibers produced from CTMP of white birch were broken pieces or fragments of fiber surfaces or cell corners. The lignin was observed in the fibers until 5min of ozone treatment but no lignin was observed after 5 minutes of ozone treatment. Different morphological characteristics of TMP and CTMP explained both the different morphological characteristics and the distribution of lignin observed in the fine fibers produced from the beating process of TMP and CTMP treated with ozone.

• 한국미생물·생명공학회지
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• 제24권2호
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• pp.149-154
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• 1996

Effect of glucose addition to xylose medium on xylitol production was investigated by using Candida parapsilosis ATCC 21019 mutant. With increasing the ratio of glucose to xylose in total amount of 50 g/l as glucose and/or xylose, xylitol production was decreased but ethanol and glycerol production were increased. Ethanol and glycerol concentration were maxmum in 10 g/l of xylose and 40 g/l of glucose medium as 21.5 g/l and 3.6 g/l, respecti- vely. No xylitol was formed in glucose medium without xylose because xylitol could be not produced from glucose. With increasing the ratio of glucose to xylose, the activity of xylose reductase which converted xylose to xylitol were decreased. The activities of xylitol dehydrogeiiase which converted xylitol to xylulose and then cell materials were found to be constant regardless of the ratio of glucose to xylose. This results indicated that glucose addition to xylose medium on cell growth was not affected. In order to prevent the inhibitory effect of glucose on xylitol production, glucose in a fermentor was fed with low concentration and then ethanol and glycerol was critically decreased and the xylitol yield from xylose of the culture with glucose feeding was recovered the almost same as that with only 50 g/l of xylose. However, the xylitol yield from total sugars (xylose and glucose) was decreased and glucose was not contributed to xylitol production. Therefore, the fermentation at high concentration of xylose without glucose was carried out. A final xylitol concentration of 242 g/l which corresponding 80.7% of xylitol yield was obtained from 300 g/l of xylose for 273 hours.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권5호
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• pp.269-278
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• 2003

Gibberellic acid (GA$_3$) is a commercially important plant growth hormone, which is gaining much more attention all over the world due to its effective use in agriculture and brewing industry. Industrially it is produced by submerged fermentation technique using Ascomycetous fungus Gibberella fujikuroi. Solid state and immobilized cell fermentation techniques had also been developed as an alternative to obtain higher yield of GA$_3$. This review summarizes the problems of GA$_3$ fermentation such as production of co-secondary metabolites along with GA$_3$, substrate inhibition and degradation of GA$_3$ to biologically inert compound gibberellenic acid, which limits the yield of GA$_3$ in the fermentation medium. These problems can be overcome by various bioprocessing strategies e.g. two - stage and fed batch cultivation processes. Further research on bioreactor operation strategies such as continuous and / or extractive fermentation with or without cell recycle / retention system need to be investigated for improvement in yield and productivity. Down stream processing for GA$_3$ isolation is also a challenge and procedures available for the same have been critically evaluated.