• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.37 no.5 s.113
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• pp.25-34
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• 2005

The chemical composition of the differentiating xylem of Populus deltoides M. was investigated and compared with that of sapwood. The cell wall polysaccharides were extracted sequentially from adifferentiating xylem and fractionated with gel chromatography. The sugar composition of each fraction was analyzed with G..C and H.P.L.C. The cell wall of the differentiating xylem is rich with the pectin substance and hemicellulose compared with that of sapwood. The water-extracted polysaccharides from the differentiating xylem were composed mainly of xylose and glucose residues. The sugar composition of some of the fractions in the gel filtration of purified $H_{2}O$ polysaccharide suggest that xyloglucan was extracted with $H_{2}O$ from differentiating xylem. Also, we can supposed that the purified $H_{2}O$ polysaccharide might be xyloglucan from the spectrometric data(IR and NMR) of purified $H_{2}O$ polysaccharide.

• BioChip Journal
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• v.12 no.4
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• pp.287-293
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• 2018

Bacillus cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBD-MNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and $10^3CFU\;per\;mL$ in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.

• Journal of the Korean Wood Science and Technology
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• v.48 no.6
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• pp.780-790
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• 2020

In this study, the cell-wall microstructure and anti-swelling effectiveness (ASE) of heat-treated larch wood were evaluated and the correlation between them was analyzed. For this purpose, some larch lumbers were heat-treated for 12, 18, and 24 hours at temperatures of 190℃ and 220℃. By observing the scanning electron microscopy cross-sectional image of the heat-treated larch, it was confirmed that the shape of heat-treated wood cell changed, the cut-section of the wood cell wall was rough, and the intercellular space has become wide as the intercellular bonds had broken because of heat-treatment. In addition, the evaluation of the swelling for each treatment condition revealed that, as the heat-treatment temperature and duration increased, the amount of absorbed water and swelling decreased and the ASE increased. The decrease in the amount of absorbed water is thought to be affected by the chemical change in the cell wall by heat-treatment. On the contrary, the decrease in the swelling and the increase in the ASE are thought to be due to a combination of chemical changes and physical changes such as structural changes in the cell wall.

• Applied Biological Chemistry
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• v.30 no.4
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• pp.300-304
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• 1987

Effects of benzyladenine (BA) on viability, cell wall regeneration and division of soybean (Glycine max, Var. Acme) protoplasts isolated from suspension cells of cotyledonary callus were investigated. The uptake of BA by the protoplasts was also studied. BA increased protoplast viability, and promoted cell wall regeneration and cell division. The level of BA in protoplasts was increased to a maximum at about 20 hour incubation and 2/3 of the total amount of BA accumulated in protoplast was absorbed within 6 hours.

• Journal of the Korean Wood Science and Technology
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• v.34 no.5
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• pp.1-10
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• 2006

The objective of this study was to elucidate the microscopic patterns of decay caused by brown-rot fungi of Tyromyces palustri and Gloeophyllum trabeum in Korean red pine (Pinus densiflora) and radiata pine (Pinus radiata) woods through light and electron microscopies. The ultrastructural changes of cell walls attacked by the two brown-rot fungi were compared in this respect. Macroscopically, radiata pine showed more ring and radial checks than Korean red pine. Microscopically, with the progress of decay, spiral checks associated with cross-field pits and bore holes in the cell wall were more remarkably numerous in the radiata pine than in the Korean red pine. In the radiata pine, G. trabeum produced more spiral checks in the cell wall than T. palustris. In the advanced stages of decay by G. trabeum, the erosions of ray cell walls were identified both in the Korean red pine and radiata pine but $S_3$ layers of tracheid walls were eroded only in the Korean red pine.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.72-78
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• 2001

It is possible ot prepare protoplasts of the zygomycete fungus, Phycomyces blakesleeanus, by digesting the cell wall of spore germlings with commercially available chitinase and chitosanase. However, the cells without any cell walls immediately form large aggregates, and thus, it is difficult to isolate the individually separated protoplasts. Inherent problem with the formation of aggregates in preparing protoplasts could be solved by the use of bovine serum albumin (BSA). As a result, we were able to prepare a large number of single protoplsts quickly and easily. We took time-lapse photomicrographs of the formation of protoplasts, and found that there were certain regions of the cell wall of spore germlings that were sensitive to chitinase and chitosanase, although the cell wall of the original spores is known to be insensitive to these enzymes. There are two kinds of cell walls on a spore germling; one with a bound wheat germ agglutinin (WGA), and the other a bound concanavalin A (ConA). Furthermore, only cells with walls which had bound WGA were able to regenerate, while those with walls with bound ConA were not able to regenerate.

• Proceedings of the IEEK Conference
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• 2000.06e
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• pp.77-80
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• 2000

This paper describes a new method to decide the margin for the sustain voltage of AC PDPs based on the wall-charge distribution. We model the discharge cell and measure the wall-charge when sustain pulses are applied to the AC PDP. The measured wall-charge distribution informs us of the voltage forming the maximum wall-charge which should be chosen as the sustain voltage.

• Microbiology and Biotechnology Letters
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• v.27 no.1
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• pp.8-14
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• 1999

This study was developed to evaluate the growth inhibition effects of cell wall components of Enterococcus faecalis 2B4-1 obtained from feces of neonates against tumor cell lines. Polysaccharide fraction (PS) shown sensitive growth inhibition effect in the cell wall components was isolated and characterized. In growth inhibition effects, residue fractin of whole cell was shown sensitive level of percent survival about 30% when administrated at ehe concentration of 100${\mu}$g/ml, and that was more effective than that of supernatant fraction against the tumor cell lines, SNU-1, 3LL, FARROW and HEC-1-B. Sensitive growth inhibition effects against SNU-1, FARROW and HEC-1-B were performed by whole cell (WC) fraction from Ent. faecalis 2B4-1. Cytoplasm fractin (CP) of WC was shown non-inhibition effect, however, the other part of WC, precipitate of disrupted cell (PD), was sensitive against the tumor cell line mentioned above. Followed by separation to peptidoglycan fraction (PG) and polysaccharide fraction (PS) were all sensitive which the latter was shown more sensitive percent survival than the former. Composed sugars of polysaccharide fraction were determined to D-glucose, L-rhamnose and D-glucosamine, and the rate fo composition was calculated to about 1:1:1 by the data of elemental analysis, IR, TLC and HPLC.

• Journal of Chest Surgery
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• v.25 no.7
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• pp.707-710
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• 1992

Percutaneous needle aspiration has been widely used in the diagnosis of pulmonary lesions, because it is a fairly simple procedure with good diagnostic accuracy and low complication rate. Among its complications, the spread of malignant cells along the needle tract is rare but serious one. We report a case of chest wall implantation of lung cancer after the percutaneous fine needle aspiration biopsy. A 57-year-old man had undergone a right upper lobectomy for squamous cell carcinoma [T2N0M0] of the lung, 3 months after the operation, a growing mass, located far from the previous thoracotomy incision, developed on the right anterior chest wall where the diagnostic thin needle biopsy had been performed before the lobectomy. A wide excision of the chest wall mass was performed, and permanent histology showed squamous cell carcinoma as noted before.

• Journal of the Korean Society of Industry Convergence
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• v.19 no.3
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• pp.109-124
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• 2016

The objective of this paper is to find the density, stiffness, and strength of truss-wall diamond corrugation model combined with pinwheel truss inside space. The truss-wall diamond corrugation (TDC) model is defined as a unit cell coming from solid-wall diamond corrugation (SDC) model. Pinwheel truss-wall diamond corrugation (P-TDC) model is made by TDC connected with pinwheel structure inside of the space. Derived ideal solutions of P-TDC is based on truss-wall and pinwheel truss model at first. And then it is compared with Gibson-Ashby's ideal solution. To validate the ideal solutions of the P-TDC, ABAQUS software is used to predict the density, strength, and stiffness, and then each of them are compared to the ideal solution of Gibson-Ashby with a log-log scale. Applied material property is stainless steel 304 because of having cost effectiveness. Applied parameters for P-TDC are 1 thru 5 mm diameter within fixed opening width as 4mm. In conclusion, the relative Young's modulus and relative yield strength of the P-TDC unit model is reasonable matched to the ideal expectations of the Gibson-Ashby's theory. In nearby future, P-TDC model is hoped to be applied to make sandwich core structure by advanced technologies such as 3D printing skills.