Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of Cytokinin on Cell Wall Regeneration and Cell Division of Soybean Protoplasts

대두 Protoplast의 세포벽 합성과 세포분열에 대한 Cytokinin의 영향

  • Yoo, Ki-Jung (Department of Agricultural Chemistry, Cheju National University) ;
  • Kim, Hyoeng-Ok (Department of Agricultural Chemistry, Cheju National University) ;
  • Park, Chang-Kyu (Department of Agricultural Chemistry, Seoul National University) ;
  • Kim, Chang-Oh (Department of Agricultural Chemistry, Cheju National University)
  • Published : 1987.12.30
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Abstract

Effects of benzyladenine (BA) on viability, cell wall regeneration and division of soybean (Glycine max, Var. Acme) protoplasts isolated from suspension cells of cotyledonary callus were investigated. The uptake of BA by the protoplasts was also studied. BA increased protoplast viability, and promoted cell wall regeneration and cell division. The level of BA in protoplasts was increased to a maximum at about 20 hour incubation and 2/3 of the total amount of BA accumulated in protoplast was absorbed within 6 hours.

대두(Glycine max var. Acme)의 자엽조직에서 유래된 callus의 부유배양세포로부터 protoplast를 분리하고, 이들 protoplast의 viability, 세포벽재생 및 세포분열에 대한 benzyladenine(BA)의 영향과 protoplast에 의한 BA의 흡수특성을 조사하였다. Protoplast의 viability는 BA처리에 의하여 증가되었고, 세포벽재생과 세포분열 그리고 callue의 생장도 BA처리에 의하여 촉진되었다. Protoplast에 의하여 흡수된 BA의 양은 BA 처리후 약 20시간에 최대에 이르렀고, 이중에서 약 2/3가 6시간 이내에 흡수되었다.

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