• 제목/요약/키워드: cell wall thickness

검색결과 121건 처리시간 0.038초

마이크로 PIV를 이용한 미세튜브 내부 조류 혈액유동에 관한 실험적 연구 (Experimental Investigation on Flow Characteristics of Chicken Blood in a Micro Tube Using a Micro-PIV Technique)

  • 여창섭;지호성;이상준
    • 대한기계학회논문집B
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    • 제30권11호
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    • pp.1027-1034
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    • 2006
  • In order to investigate flow characteristics of chicken blood in a micro tube of 100$\mu$m in diameter, in-vitro experiments were carried out using a micro-PIV technique. The micro-PIV system consists of a microscope, 2-head Nd:YAG laser, 12 bit cooled CCD camera and a delay generator. Chicken blood with 40% hematocrit was supplied into a micro tube using a syringe pump. The blood flow shows clearly the cell free layer near the tube wall and its thickness is increased with increasing the flow speed. The hemorheological characteristics of chicken blood, including shear rate and shear stress were estimated from the PIV velocity field data obtained. Since the aggregation index of chicken blood is less than 50% of human blood, non-Newtonian flow characteristics of chicken blood are smaller than those of human blood. As the flow rate increases, the degree of flatness in the velocity profile at the center region is decreased and the parabola-shaped shear stress distribution becomes to have a linear profile. Under the same flow rate, chicken blood shows higher shear stress, compared with human blood.

표면에 다수의 구멍을 뚫고 차단 주파수 영역의 선택이 가능한 풍압저감형 방음벽 (Wind Pressure Reducing Soundproof Wall Which Has Many Holes on the Surface and Selectable Stop-Frequency Ranges)

  • 김상훈;이돈출
    • 대한토목학회논문집
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    • 제36권2호
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    • pp.191-196
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    • 2016
  • 파동의 에돌이와 공명식 머플러 원리를 이용하여 통풍형 방음을 구현하고 그 이론을 설명하였다. 이 방음기술은 차단하고자 하는 주파수영역의 선택이 가능하다. 방음벽을 이중으로 만들고 그 사이를 비워, 방음벽의 구멍을 통해 들어온 음파가 내부에서 공진하여 소멸되도록 하였다. 두께 8cm인 공진통마다 직경 5cm의 공기구멍을 2개씩 뚫고 실험한 결과 넓은 주파수영역대서 평균 25dB정도의 차음효과가 있었다. 일본 컴넥스사의 산란에 의한 투과손실만 있는 경우와의 차음효과를 비교한 결과 같은 통풍능력인 경우에 적어도 10dB이상 차음효과가 높았다. 또한 컴넥스사의 기술은 차음주파수 영역을 선택할 수 없다는 한계가 있었다.

Structural Differentiation of the Connective Stalk in Spirodela polyrhiza (L.) Schleiden

  • Kim, InSun
    • Applied Microscopy
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    • 제46권2호
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    • pp.83-88
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    • 2016
  • Structural differentiation of the connective stalk in giant duckweed, Spirodela polyrhiza, was examined to reveal the anatomical and ultrastructural characteristics within reduced shoot. The study focuses primarily on structural features of the connective stalk (CT), which connect offspring to their mother fronds. Photoautotrophic offspring fronds remained connected by stalks to mother fronds in the reproductive pockets until separation. The CT originated from the meristematic region of the abaxial frond and joined the fronds laterally with two abscission layers. The most notable features of the CT were polymorphic mitochondria, random occurrences of fibrillar structures in intercellular spaces, and great variability in cell wall thickness. Vascular tissues in CTs were highly reduced, demonstrating only a central vascular strand. Grana with 2 to 4 thylakoids and starch grains were found in the chloroplasts. A chlorophyll assay indicated high chlorophyll concentrations in daughter fronds and low concentrations in CTs. The frond and CT, while physically connected to each other, functioned independently. Despite great reduction in S. polyrhiza, the CT has proven to be very efficient for separating offspring from the mother frond, which lends to its capacity for rapid vegetative reproduction. The ultrastructural aspects of CTs in S. polyrhiza were characterized for the first time in this study.

건지에서 자란 잣나무(Pinus koraiensis S. et Z.)의 이기목부 및 형성층구조의 변화 (Structure of Secondary Xylem and Cambial Initials in Pinus koraiensis S. et Z. Grown in Arid Soil)

  • 임동옥
    • Journal of Plant Biology
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    • 제36권1호
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    • pp.35-42
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    • 1993
  • This study deals with the effect of dwarf growth for the wood and the structural changes of cambium in Pinus koraiensis subjected to water stress. The length and wall thickness of tracheary elements in dwarf trees was shorter and thicker than that in normal trees. The redial width and cell number of the annual rings are narrower and smaller in dwarf trees than those in normal trees. In serial tangential sections, the frequency of anticlinal division is low and loss of cambial initials is small in dwarf trees. The length of cambial initials in water deficit, trees are slightly shorter than that in normal trees, and the instructive growth of cambial initials during differentiation of xylem mother cells is inactive in dwarf trees. Thus, it is interpreted that the shortening of tracheary elements in dwarf trees is due to the fact that the length of cambial initials are shortened and the intrusive growth of those during differentiation of xylem mother cells is inactive. The structural variations in the wood of naturally occurring dwarf trees are similar to those of trees subjected to artificial water stress. Therefore, it is suggested that the variation of xylem element in dwarf trees are related to water conditions.

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$AgInS_2$ 단결정 박막 성장과 광센서 특성 (Growth and Photosensor Properties for $AgInS_2$ Single Crystal Thin Film)

  • 홍광준;백승남
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2006년도 하계학술대회 논문집 Vol.7
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    • pp.134-135
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    • 2006
  • $AgInS_2$ single crystal thin filmsl was deposited on throughly etched semi-insulator GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were $680^{\circ}C$ and $410^{\circ}C$ respectively, and the thickness of the single crystal thin films is $6{\mu}m$. From the photocurrent spectrum by illumination of perpendicular light on the c-axis of the $AgInS_2$ single crystal thin film, we have found that the values of spin orbit coupling ${\Delta}So$ and the crystal field splitting ${\Delta}Cr$ were 0.0098 eV and 0.15 eV at 10 K, respectively. In order to explore the applicability as a photoconductive cell, we measured the sensitivity ($\gamma$), the ratio of photocurrent to darkcurrent (pc/dc), maximum allowable power dissipation (MAPD), spectral response and response time. The result indicated that the samples annealed in S vapour the photoconductive characteristics are best. Therefore we obtained the sensitivity of 0.98, the value of pc/dc of $1.02{\times}10^6$, the MAPD of 312 mW, and the rise and decay time of 10.4ms and 10.8ms respectively.

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Protective Effect of Right Ventricular Mitochondrial Damage by Cyclosporine A in Monocrotaline-induced Pulmonary Hypertension

  • Lee, Dong Seok;Jung, Yong Wook
    • Korean Circulation Journal
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    • 제48권12호
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    • pp.1135-1144
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    • 2018
  • Background and Objectives: Mitochondria play a key role in the pathophysiology of heart failure and mitochondrial permeability transition pore (MPTP) play a critical role in cell death and a critical target for cardioprotection. The aim of this study was to evaluate the protective effects of cyclosporine A (CsA), one of MPTP blockers, and morphological changes of mitochondria and MPTP related proteins in monocrotaline (MCT) induced pulmonary arterial hypertension (PAH). Methods: Eight weeks old Sprague-Dawley rats were randomized to control, MCT (60 mg/kg) and MCT plus CsA (10 mg/kg/day) treatment groups. Four weeks later, right ventricular hypertrophy (RVH) and morphological changes of right ventricle (RV) were done. Western blot and reverse transcription polymerase chain reaction (RT-PCR) for MPTP related protein were performed. Results: In electron microscopy, CsA treatment prevented MCT-induced mitochondrial disruption of RV. RVH was significantly increased in MCT group compared to that of the controls but RVH was more increased with CsA treatment. Thickened medial wall thickness of pulmonary arteriole in PAH was not changed after CsA treatment. In western blot, caspase-3 was significantly increased in MCT group, and was attenuated in CsA treatment. There were no significant differences in voltage-dependent anion channel, adenine nucleotide translocator 1 and cyclophilin D expression in western blot and RT-PCR between the 3 groups. Conclusions: CsA reduces MCT induced RV mitochondrial damage. Although, MPTP blocking does not reverse pulmonary pathology, it may reduce RV dysfunction in PAH. The results suggest that it could serve as an adjunctive therapy to PAH treatment.

Anatomical Structures and Fiber Quality of Four Lesser-Used Wood Species Grown in Indonesia

  • MARBUN, Sari Delviana;WAHYUDI, Imam;SURYANA, Jajang;NAWAWI, Deded Sarip
    • Journal of the Korean Wood Science and Technology
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    • 제47권5호
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    • pp.617-632
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    • 2019
  • This study aimed to investigate the anatomical structure and fiber quality of four lesser-used wood species namely Benuang (O. sumatrana), Duabanga (D. moluccana), Pisang Merah (H. hellwigii), and Terap (A. odoratissimus). This study evaluated its suitability for raw material in pulp and paper manufacturing. The anatomical structure was observed macro- and microscopically. Macroscopic structures were observed directly to the wood samples, while microscopic characteristics were observed through microtome specimens. Fiber dimension was measured through macerated specimens and fiber quality was analyzed following the Rachman and Siagian's method. Results showed that these four timber species have similarity in the indistinct growth ring, diffuse porous in a radial pattern, rounded solitary vessel outline, 1 to 3 cells of ray width, deposits within the rays, fiber length, and cell wall thickness. Differences were found on vessel diameter, vessel grouping, vessel frequency, tyloses existence, type of axial parenchyma, and ray height. Based on fiber length and its derived values, the wood fibers of all species studied are suitable for pulp and paper manufacturing. They belong to the II quality class. The produced pulp and paper would have good quality, especially in tensile, folding, and tear strength. To promote their utilization, silviculture aspect of these four species has to be well understood.

The Interkingdom Interaction with Staphylococcus Influences the Antifungal Susceptibility of the Cutaneous Fungus Malassezia

  • Juan Yang;Sungmin Park;Hyun Ju Kim;Sang Jun Lee;Won Hee Jung
    • Journal of Microbiology and Biotechnology
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    • 제33권2호
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    • pp.180-187
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    • 2023
  • The skin is a dynamic ecosystem on which diverse microbes reside. The interkingdom interaction between microbial species in the skin microbiota is thought to influence the health and disease of the skin although the roles of the intra- and interkingdom interactions remain to be elucidated. In this context, the interactions between Malassezia and Staphylococcus, the most dominant microorganisms in the skin microbiota, have gained attention. This study investigated how the interaction between Malassezia and Staphylococcus affected the antifungal susceptibility of the fungus to the azole antifungal drug ketoconazole. The susceptibility was significantly decreased when Malassezia was co-cultured with Staphylococcus. We found that acidification of the environment by organic acids produced by Staphylococcus influenced the decrease of the ketoconazole susceptibility of M. restricta in the co-culturing condition. Furthermore, our data demonstrated that the significant increased ergosterol content and cell membrane and wall thickness of the M. restricta cells grown in the acidic environment may be the main cause of the altered azole susceptibility of the fungus. Overall, our study suggests that the interaction between Malassezia and Staphylococcus influences the antifungal susceptibility of the fungus and that pH has a critical role in the polymicrobial interaction in the skin environment.

APPLICATION OF TIME-OF-FLIGHT NEAR INFRARED SPECTROSCOPY TO WOOD

  • Tsuchikawa, Satoru;Tsutsumi, Shigeaki
    • 한국근적외분광분석학회:학술대회논문집
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    • 한국근적외분광분석학회 2001년도 NIR-2001
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    • pp.1182-1182
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    • 2001
  • In this study, the newly constructed optical measurement system, which was mainly composed of a parametric tunable laser and a near infrared photoelectric multiplier, was introduced to clarify the optical characteristics of wood as discontinuous body with anisotropic cellular structure from the viewpoint of the time-of-flight near infrared spectroscopy (TOF-NIRS). The combined effects of the cellular structure of wood sample, the wavelength of the laser beam λ, and the detection position of transmitted light on the time resolved profiles were investigated in detail. The variation of the attenuance of peak maxima At, the time delay of peak maxima Δt and the variation of full width at half maximum Δw were strongly dependent on the feature of cellular structure of a sample and the wavelength of the laser beam. The substantial optical path length became about 30 to 35 times as long as sample thickness except the absorption band of water. Δt ${\times}$ Δw representing the light scattering condition increased exponentially with the sample thickness or the distance between the irradiation point and the end of sample. Around the λ=900-950 nm, there may be considerable light scattering in the lumen of tracheid, which is multiple specular reflection and easy to propagate along the length of wood fiber. Such tendency was remarkable for soft wood with the aggregate of thin layers of cell walls. When we apply TOF-NIRS to the cellular structural materials like wood, it is very important to give attention to the difference in the light scattering within cell wall and the multiple specular-like reflections between cell walls. We tried to express the characteristics of the time resolved profile on the basis of the optical parameters for light propagation determined by the previous studies, which were absorption coefficient K and scattering coefficient S from Kubelka-Munk theory and n from nth power cosine model of radiant intensity. The wavelength dependency of the product of K/S and n, which expressed the light-absorbing and -scattering condition and the degree of anisotropy, respectively, was similar to that of the time delay of peak maxima Δt. The variation of the time resolved profile is governed by the combination of these parameters. So, we can easily find the set of parameters for light propagation synthetically from Δt.

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배추 조직감 측정 방법의 최적화와 품종 간 물성특성비교 (Optimizing a Method for Measuring Firmness of Chinese Cabbage (Brassica rapa) and Comparing Textural Characteristics among Cultivars)

  • 정준혁;이영섭;김종기
    • 원예과학기술지
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    • 제30권6호
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    • pp.700-708
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    • 2012
  • 배추의 조직감 측정 방법을 확립하기 위하여 4종류의 탐침을 사용하여 '노랑봄' 배추의 엽서별 중륵의 경도를 비교 측정하였다. 이중에서 volodkevich bite jaws 탐침으로부터 엽서와 경도의 상관이 가장 높은 유의성이 나타났다($r^2=0.85$). 배추 중륵의 조직감은 단위두께당 조직파쇄에 소요되는 힘으로 나타냈다. 배추의 작형과 생태형을 고려하여 16 품종의 엽서별 중륵 부위의 경도를 측정하였다. 중륵의 두께 분포는 외엽부 수개의 잎에서 증가하다가 내엽으로 갈수록 감소하였으며, 중륵의 평균두께는 'CR-싱싱'이 7.74mm로 가장 얇고, '노랑여름' 배추는 9.28mm로 가장 두꺼웠다. 잎두께의 변이는 '치힐리'가 23.6%로 가장 높았으며, '불암' 배추는 5.8%로 가장 적었다. 중륵의 조직감은 모든 품종에서 외엽에서 일시적으로 감소하다가 내엽으로 갈수록 증가하는 2차 함수적인 관계를 보였고, 결정계수는 16품종 중 13품종에서 0.75 이상이었다. 'CR-그린'은 0.23으로 가장 낮았고, '치힐리' 배추는 0.96으로 적합성이 가장 높았다. 수확한 배추의 조직감을 신속하게 평가하기 위하여는 제10번 또는 제11번 잎의 중륵의 경도를 측정하는 방법을 제시하였으며, 16품종의 평균결정계수는 0.81이었다. 중륵의 건물중과 경도는 정의 상관($r=0.70^{**}$)을 보였고, 세포벽 함량과 경도와의 상관은 다소 낮았지만 유의성이 있었다($r=0.58^*$). 본 연구에서 얻어진 배추의 조직감 측정기술은 원료배추와 김치의 조직감의 관계를 구명하거나 조직감이 우수한 배추품종을 육성하는 데 활용할 수 있다고 사료되었다.