• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.3
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• pp.359-367
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• 1999

For investigating the activities of algicidal bacteria, the variations of algicidal bacterial population and chlorophyll-a were checked weekly in Chindong Bay, Korea from May to July, 1998. For identifying their killing characteristics, three strains were selected from the isolated algicidal bacteria. The density of algicidal bacteria kept changing in the range of $6.0\times10^1$ to $6.4\times10^5$ cell $\ell^{-1}$. The density flux of algicidal bacteria coincided with that of chlorophyll-a by a week of lag time. Three algicidal bacteria isolated from field strains, H519S5-4, H605S5-15 and H605S5-22, were investigated in nine microalgal species, Heterosigma akashiwo, Chattonella sp. (Raphidlphyceae), Gymnodinium catenatum, Gyrodinium impudicum, Cochlodinium polyklikoides (Dinophyceae), Chaetoceros sp., Coscinodiscus granii, Ditylum brightwellii, Thalassiosira rotula (Bacillariophyceae). Strain H605S5-22 showed a wide algicidal activities over nine microlgae, strain H605S5-15 over H. akashiwo, G. catenatum, T. rotula, Chattonella sp. and strain H519S5-4 over H. akashiwo, Chattonella sp., Chaetoceros sp., G. catenatum. The activities of the three strains were detected by the secretion of algicidal substances. Therefore, it is suggested that the activities of algicidal bacteria have a significant influence over the population dynamics of phytoplankton and get involved with the sharp decrease in red tides in the coastal area.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.6
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• pp.605-610
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• 2001

We investigated the effects of salinity on an estuarine cladoceran, Diaphanosoma celebensis as a substitute live food organism of Artemia, which has been used for the next step of rotifer, in the course of seedling production of marine fishes, Culture density for growth, possibility of stable culture (RPGI: Relative Population Growth Index) and food feeding activity (RCN: Remaining Cell Numbers) of D. celebensis were investigated at intervals of 5 ppt under 10 steps of different saline conditions from 0 to 45 ppt. According to the results, the experimental group, which was regarded as the best condition of culture density for growth, stable culture possibility (RPGI) and good food feeding activity (RCN), was salinity culture condition of 20 put, and relative stable culture conditions were observed under saline concentrations $15\~35\;ppt$. Their functions under culture conditions of lower or higher salinities than 20 ppt appeared remarkably to be decreased, while all individuals were dead under the saline condition of 0 ppt after the beginning of the experiment. From these results of this study, it is assumed that estuarine cladoceran, D. celebensis can be cultured easily as a substitute live food organism of Artemia in the course of seedling production of marine fishes.

• The Plant Pathology Journal
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• v.30 no.3
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• pp.288-298
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• 2014

We examined the efficacy of a bacterium for biocontrol of the root-knot nematode (RKN) Meloidogyne hapla in carrot (Daucus carota subsp. sativus) and tomato (Solanum lycopersicum). Among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments with isolate C1-7, which was identified as Bacillus cereus based on cultural and morphological characteristics, the Biolog program, and 16S rRNA sequencing analyses. The population density and the nematicidal activity of B. cereus C1-7 remained high until the end of culture in brain heart infusion broth, suggesting that it may have sustainable biocontrol potential. In pot experiments, the biocontrol efficacy of B. cereus C1-7 was high, showing complete inhibition of root gall or egg mass formation by RKN in carrot and tomato plants, and subsequently reducing RKN damage and suppressing nematode population growth, respectively. Light microscopy of RKN-infected carrot root tissues treated with C1-7 showed reduced formation of gall cells and fully developed giant cells, while extensive gall cells and fully mature giant cells with prominent cell wall ingrowths formed in the untreated control plants infected with RKNs. These histopathological characteristics may be the result of residual or systemic biocontrol activity of the bacterium, which may coincide with the biocontrol efficacies of nematodes in pots. These results suggest that B. cereus C1-7 can be used as a biocontrol agent for M. hapla.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1661-1677
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• 2006

Rhizobium and related genera are soil bacteria with great metabolic plasticity. These microorganisms survive in many different environments and are capable of eliciting the formation of nitrogen-fixing nodules on legumes. The successful establishment of symbiosis is precisely regulated and requires a series of signal exchanges between the two partners. Quorum sensing (QS) is a prevalent form of population density-dependent gene regulation. Recently, increasing evidence indicates that rhizobial quorum sensing provides a pervasive regulatory network, which plays a more generalized role in the physiological activity of free-living rhizobia, as well as during symbiosis. Several rhizobia utilize multiple, overlapping quorum sensing systems to regulate diverse properties, including conjugal transfer and copy number control of plasmids, exopolysaccharide biosynthesis, rhizosphere-related functions, and cell growth. Genomic and proteomic analyses have begun to reveal the wide range of functions under quorum-sensing control.

• Ocean and Polar Research
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• v.34 no.1
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• pp.65-71
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• 2012

Gambierdiscus toxicus, Adachi et. Fukuyo, is a benthic ciguatoxin-producing armored dinoflagellate, often attached to macroalgae. This organism is the primary causative agent of ciguatera fish poisoning which occurs in tropical and subtropical regions. However, regardless of the fact that the population of Gambierdiscus spp. has expanded to such temperate areas from sub-trophic and trophic areas, monitoring of G. toxicus has been lacking in the Korean coastal waters of temperate areas. This study was performed at the uninhabited Baekdo Islands off the southern coast of Korea and at Seopsom Island in the vicinity of Seogwipo, Jeju Province during April and May, 2011. Cell densities of Gambierdiscus spp. on macroalgae at Baekdo and Jeju Island ranged from zero to 56.4 cells $g^{-1}$. Maximum density was recorded on the brown alga Cladophora japonica at St. 3 of Jeju Island. In particular, the cell densities of Gambierdiscus spp. were influenced by the substrate characteristics of macroalgae. In the future, the continuous monitoring of toxic benthic dinoflagellate is necessary to predict and prevent ciguatera poisoning in Korean coastal waters.

• Korean Journal of Environmental Biology
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• v.18 no.1
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• pp.95-104
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• 2000

In order to elucidate chatracteristics of water quality and seasonal dynamics of phytoplankton community in Kyungpo lake, the weekly variation of algal population and 11 environmental factors were investigated from March 1998 to February 1999. A total 121 species of phytoplankton belonged to 57 genera, 4 genera 7 species of the Euglenophyceae, 1 genera 2 species of the Chrysophyceae, 2 genera 3 species of the Cryptophyceae, 1 genera 1 species of the Dinophyceae, 6 genera 9 species of the Cyanophyceae, 28 genera 78 species of the Bacillario-phyceae and 15 genera 21 species of the Chlorophyceae, was identified. Cryptomonas sp., Chamydomonas sp., Nitzschia frustulum, Oscillatoria sp., Lyngbya sp, and Dictyosphaerium pulchellum occurred successively as dominant phytoplankton species from March 1998 to February 1999. Density of phytoplankton cell number showed the peak in early April when Chlamydomoas sp. was dominated. The diversity index of phytoplankton community showed low values in the dominating period of Cryptomonas sp. and Chlamydomonas sp. The concentration of chlorophyll a fluctuated between 1.09 and 107.7$\mu\textrm{g}$ㆍ1$^{-1}$. Water temperature, salinity, DO, conduc tivity and SS were ranged 0.5-27.8$^{\circ}C$, 2.8-25.2 ppt, 4.62-19.21 mgO$_2$ㆍ1$^{-1}$, 5.33-34.76 mS/cm and 0.017-0.184 mgㆍ1$^{-1}$, respectively. Annual means of Secchi disc transparency was 36cm and thai of pH value was 8.5. The concentrations of PO$_4$-P was ranged 0.0012-0.077 mgㆍ1$^{-1}$ , and showed high value from August to October. The concentrations of NO$_2$-N, NO$_3$-N and NH$_4$-N was ranged 0.0011-0.1 mgㆍ1$^{-1}$, 0.074-3.33mgㆍ1$^{-1}$ and 0.0024-0.259 mgㆍ1$^{-1}$, respectively. Among environmental factors, water temperature was negatively correlated with DO, salinity and conductivity, DO was positively correlated with salinity and conductivity. Chlorophyll a, one of the indicies of eutropication in lake, was negatively correlated with NH$_4$-N. The cell density of Cryptomonas sp., which was the most dominant species in this lake, was negatively correlated with transparency. [Kyoungpo lake, Phytoplankton, Cryptomonas sp. , Chlamydomonas sp., Seasonal variation].

• Journal of Ginseng Research
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• v.12 no.2
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• pp.104-113
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• 1988

Effect of the purified ginsenoside $-Rb_1$ and $-Rb_2$ on LDL receptor biosynthesis of CHO cell cultured in a high cholesterol medium was investigated . Cholesterol uptake by CHO cell cultured in a medium containing various amounts of cholesterol was traced and found that the cholesterol uptake was proportional to the concentration of cholesterol in the medium, and the population of LDL receptors were proportionally decreased as the increasing cholesterol level in the cell. However, when the CHO cells were cultured in the medium containing ginsenosides, no significant decrease of LDL receptor population occured. The biosynthesis of protein and RNA of the above cells was higher than that of CHO cells cultured in the absence of the ginsenosides, suggesting that the ginsenosides might stimulate LDL receptor bio-synthesis. It was also observed that the ginsenosides stimulated the biosynthesis of estradiol and progesterone from cholesterol in the CHO cell. From the above results, it seemed that the ginsenosides lowers the cholesterol level by stimulating the cholesterol metablism including steroid hormone biosynthesis, resulting in the lowering of inhibitory action of cholesterol on LDL receptor biosynthesis.

• Fisheries and Aquatic Sciences
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• v.3 no.1
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• pp.26-32
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• 2000

Monospecific red tide by a toxic dinoflagellate belonging to the genus Alexandrium occurred at Chindong Bay in the southern coast of Korea and continued from April 6th to 15th in 1997. The ratio of its cell number to total phytoplankton cell number was much higher than $95\%$. This organism was identified as Alexandrium tamarense, although slight morphological differences were found comparing to the original and successive descriptions of the species. We found neither anterior nor posterior attachment pores in these cells of the bloom population. The occurrence of red tide caused by A. tamarense was first reported in Korea. Its plate formula is Po, Pc, 4', 6"c, 8s, 5"' and 2"". Thecal plates are thin with pore-like ornamentation. In those plates, the anterior part of the first apical plate (1') is narrower and its posterior end has sometimes a block-like accessory, but this variation was considered within the range of the morphological variability of this taxon. The cell density during the red tide exhibited a wide range of variation by the depth of water column, ranging from $2\times10^6$ cells$l^{-1}$ to $5\times10^6$ cells·$l^{-1}$. Water temperature varied from 11.8 to $12.3^{\circ}C$. Toxicity of A. tamarense during red tide was measured as $8.8\times10^5$. $MU\;\cdot\;cell^{-1}$ by mouse bioassay.

• Nutrition Research and Practice
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• v.2 no.4
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• pp.289-294
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• 2008

Pre-B-cell leukemia transcription factor 1 (PBX1), which is located on chromosome 1q23, was recently reported to be associated with type 2 diabetes mellitus. We examined whether single nucleotide polymorphisms (SNPs) of the PBX1 gene are associated with overweight/obesity in a Korean population. We genotyped 66 SNPs in the PBX1 gene and investigated their association with clinical phenotypes found in 214 overweight/obese subjects and 160 control subjects using the Affymetrix Targeted Genotyping chip array. Seven SNPs (g.+75l86C>T, g.+78350C>A, g.+80646C>T, g.+138004C>T, g.+185219G>A, g.+191272A>C, and g.+265317T>A) were associated with the risk of obesity in three models (codominant, dominant, and recessive) (P=0.007-0.05). Haplotype 1 (CAC) and 3 (TAC) of block 3 and haplotype 2 (GGAAT) of block 10 were also strongly associated with the risk of obesity. In the control group, subjects that had homozygote for the major allele for both g.+185219G>A and g.+191272A>C showed lower high density lipoprotein-cholesterol (HDL-C) level compared to those possessing the minor allele, suggesting that the association between the homozygote for the major allele for both g.+185219G>A and g.+191272A>C and HDL-C is attributable to the increased risk of obesity. This study suggests that the PBX1 gene is a possible risk factor in overweight/obese patients.

• Journal of Environmental Science International
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• v.19 no.8
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• pp.951-960
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• 2010

Sediment works as a resource for electric cells. This paper was designed in order to verify how sediment cells work with anodic material such as metal and carbon fiber. As known quite well, sediment under sea, rivers or streams provides a furbished environment for generating electrons via some electron transfer mechanism within specific microbial population or corrosive oxidation on the metal surfaces in the presence of oxygen or water molecules. We experimented with one type of sediment cell using different anodic material so as to attain prolonged, maximum electric power. Iron, Zinc, aluminum, copper, zinc/copper, and graphite felt were tested for anodes. Also, combined type of anodes-metal embedded in the graphite fiber matrix-was experimented for better performances. The results show that the combined type of anodes exhibited sustainable electricity production for ca. 600 h with max. $0.57\;W/m^2$ Al/Graphite. Meanwhile, graphite-only electrodes produced max. $0.11\;W/m^2$ along with quite stationary electric output, and for a zinc electrode, in which the electricity generated was not stable with time, therefore resulting in relatively sharp drop in that after 100 h or so, the maximum power density was $0.64\;W/m^2$. It was observed that the corrosive reaction rates in the metal electrodes might be varied, so that strength and stability in the electric performances(voltage and current density) could be affected by them. In addition to that, COD(chemical oxygen demand) of the sediment of the cell system was reduced by 17.5~36.7% in 600 h, which implied that the organic matter in the sediment would be partially converted into non-COD substances, that is, would suggest a way for decontamination of the aged, anaerobic sediment as well. The pH reduction for all electrodes could be a sign of organic acid production due to complicated chemical changes in the sediment.